《生物技術概論》是2012年科學出版社出版的圖書，作者是王武。
基本介紹
- 中文名：生物技術概論
- 作者：王武
- 出版時間：2012年8月
- 出版社：科學出版社
- ISBN：9787030352392
內容簡介,圖書目錄,
內容簡介
本書分為基因克隆、細胞工程、酶技術、工業發酵四個部分，共21章，分別介紹了上述四大生物技術領域的基礎知識、技術關鍵以及發展前沿與動態，並對有關技術的正負面影響提出思辨。
圖書目錄
Preface
Part I Gene Cloning
Chapter 1 Introduction to Gene Cloning
1.1 Brief Overview
1.2 Historical Events
1.3 Significance and Alertness
Chapter 2 Gene and Genome
2.1 Discovery of Hereditary Factor
2.2 Basic Knowledge of Gene
2.3 Sequence Signals Involved with Gene Expression
2.4 About Genomic and Metagenomic Studies
Chapter 3 Basic Techniques for Experiments with DNA
3.1 Enzymatic Tools for Gene Manipulation
3.2 Oligo-nucleotide Tools
3.3 DNA Purification
3.4 Electrophoresis
3.5 In Vitro Synthesis of DNA
3.6 DNA Sequencing
3.7 Macromolecular Hybridization and Blotting
3.8 PCR Techniques
Chapter 4 Cloning Procedure
4.1 Basic Procedure of Cloning
4.2 Selection of Cloning Vectors
4.3 Preparation of Donor DNA
4.4 Joining of Donor and Vector by Ligation
4.5 Selection Host Cell
4.6 Introduction of Recombinant DNA into Host Cells
4.7 Selection,Screening and Sub-cloning
4.8 Derivative Technology Based on Cloning
Chapter 5 Application and Impacts
5.1 Initiation of Recombinant DNA Industry
5.2 General Aspects of Application
5.3 Contribution and Impact
Part II Cell Engineering
Chapter 6 Introduction on Cell Engineering
6.1 Brief Overview
6.2 Essentials of Cell Engineering
6.3 Progress Clues
Chapter 7 Cell Fusion and Hybridization
7.1 General Aspects
7.2 Protoplast Technique
7.3 Basic Methods of Cell Fusion
7.4 Fusant Screening
7.5 Application of Cell Fusion
Chapter 8 Plant Tissue and Cell Culture
8.1 Plant Tissue Culture System
8.2 Gene Transfer to Plants
8.3 Artificial Seeds and Virus-free Plants
Chapter 9 A nimal Tissue and Cell Culture
9.1 Establishment of Animal Tissue Culture System
9.2 Gene Transfer to Animal Cell
9.3 Animal Hybrids and Hybridoma
9.4 Approaches of Stem Cell Research
Chapter 10 Application Examples from Cell Engineering
10.1 Bioproducts from Cell Engineering
10.2 Breeding of New Agronomic Varieties
10.3 Transgenic Animals as Models for Medical Research
Part III Enzyme Technology
Chapter 11 Fundamental Nature of Enzyme
11.1 Definition,Basic Nature and Classification of Enzyme
11.2 Determination of Enzyme Activities
11.3 Factors Affecting Enzyme Activity
11.4 Resources of Enzyme
11.5 Uses of Enzyme
Chapter 12 Enzyme Biosynt hesi sand Products
12.1 Bio-resources of Enzyme
12.2 About Enzyme Synthesis
12.3 Enzyme Fermentation and Products
Chapter 13 Enzyme Recov ery & Purification
13.1 Pretreatment of Crude Enzyme Sample
13.2 Removing of Impurities and Sample Concentration
13.3 Chromatographic Separation
13.4 Polishing of Enzyme
13.5 Rational Formulation of Final Enzyme Products
Chapter 14 Enzymatic Conversion
14.1 Enzymatic Conversion
14.2 Immobilization of Enzyme and Cells
14.3 Enzymatic conversion in non-aqueous Phase
Chapter 15 New Approaches of Enzyme Technology
15.1 Modification on Enzymes
15.2 Enzyme Carriers for Immobilization
15.3 Enzyme Inhibitors
15.4 Ribozyme(Non-protein Enzymes)
Part IV Industrial Fermentation
Chapter 16 Principles of Fermentation
16.1 Fermentation Principles
16.2 Fermentation Products and Application
16.3 Benefits from the Fermentation Industry
Chapter 17 Industrial Microorganisms
17.1 Types and Characters of Industrial Microorganisms
17.2 Breeding of Industrial Strains
17.3 Industrial Microbial Growth and Culture Media
17.4 Inoculation of Industrial Strains
17.5 Preservation of Industrial Strains
Chapter 18 Microbial Metabolism and Fermentation Kinetics
18.1 Metabolism Related to Basic Pathway
18.2 Metabolic Regulation and Control
18.3 Stoichiometry of Fermentation Process
18.4 Fermentation Kinetics
Chapter 19 Bioreactors and Auxiliary Equipments
19.1 Structure and Function of Bioreactors
19.2 Auxiliary Equipment
19.3 Probes and Sensors
Chapter 20 Fermentation Down-Stream Technology
20.1 General Aspects
20.2 Harvest and Pretreatment
20.3 Isolation and Purification
20.4 Product Polishing
20.5 Waste Treatment and Cleaner Production
Chapter 21 Progress in Fermentation Engineering
21.1 Approaches of Up-stream Technology
21.2 Process Optima
21.3 Development of Down-stream Technology
Appendix
Terms Translation
Related Nobel Laureates
Further Reading
後記
