《基於超精細結構的集成微流晶片生物檢測新方法》是趙新生為項目負責人，北京大學為依託單位的青年科學基金項目。
基本介紹
- 中文名：基於超精細結構的集成微流晶片生物檢測新方法
- 項目類別：重點項目
- 依託單位：北京大學
- 項目負責人：趙新生
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 |
|---|---|---|
1 | Well positioned metallic nanostructures fabricated by nanotransfer edge printing | 期刊論文 |
2 | 鹽濃度對納米金顆粒表面DNA解鏈溫度的影響 | 期刊論文 |
3 | Domain Specific Folding Kinetics of Staphylococcal Nuclease Observed through Single-Molecule FRET in Microfluidic Mixer | 期刊論文 |
4 | Facile patterning of reduced graphene oxide film into microelectrode array for highly sensitive sensing | 期刊論文 |
5 | Processing matters: the in-situ fabrication of conducting-polymer microsensors enables ultra-low-limit gas detection | 期刊論文 |
6 | The self-assembly and patterning of thin polymer films on pyroelectric substrates driven by electrohydrodynamic instability | 期刊論文 |
7 | Measuring rapid enzymatic kinetics by electrochemical method in droplet-based microfluidic devices with pneumatic valves | 期刊論文 |
8 | 飛秒光學外差光學克爾效應和低頻拉曼光譜技術研究離子液體[bmim][PF6]的低頻光譜 | 期刊論文 |
9 | An Integrated Chip for Immunofluorescence and Its Application to Analyze Lysosomal Storage Disorders | 期刊論文 |
10 | Microfluidic etching ensure the flexible and all-solid-state supercapacitor based on sub-10-nm MnO2 nanoparticles | 期刊論文 |
11 | Kinetics and thermodynamics of DNA hybridization on gold nanoparticles | 期刊論文 |
12 | Cation and anion substitution effects on the ultrafast dynamics of interionic interaction in imidazolium based ionic liquids | 期刊論文 |
13 | 生物過程化學機制的光學單分子螢光探測 | 期刊論文 |
14 | Photophysical Properties of Atto655 Dye in the Presence of Guanosine and Tryptophan in Aqueous Solution | 期刊論文 |
15 | Kinetics and dynamics of DNA hybridization | 期刊論文 |
16 | Processing of graphen for electrochemical application: noncovalently functionalize graphene sheets with water-soluble electroactive methylene blue | 期刊論文 |
17 | Selectively discharge of electrostatics on electrets using a patterned hydrogel stamp | 期刊論文 |
18 | Self-organization of thin polymer films guided by electrostatic charges on the substrate | 期刊論文 |
19 | Patterning of electrostatic charge on electrets using hot microcontact printing | 期刊論文 |
20 | Genome-wide functional screening of miR-23b as a pleiotropic modulator suppressing cancer matastasis | 期刊論文 |
21 | fabrication of Au-based conducting polymer nanoarrays for high-performance sensor applications | 期刊論文 |
22 | Discretely Tunable Optofluidic Compound Microlenses | 期刊論文 |
23 | Interaction between Bacterial Outer Membrane Proteins and Periplasmic Quality Control Factors: A Kinetic Partitioning Mechanism | 期刊論文 |
24 | Facile fabrication of metallic nanostructures by tunable cracking and transfer printing | 期刊論文 |
25 | 1A-Adrenergic Receptor Induces Activation of Extracellular Signal-Regulated Kinase 1/2 through Endocytic Pathway | 期刊論文 |
26 | Base-by-base dynamics in DNA hybridization probed by fluorescence correlation spectroscopy | 期刊論文 |
27 | Quantitative detection of adenosine in urine using silver enhancement of aptamer-gold nanoparticle aggregation and progressive dilution | 期刊論文 |
28 | Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution | 期刊論文 |
29 | Photophysics and locations of IR125 and C152 in AOT reverse micelles | 期刊論文 |
30 | Fabrication of ultra-thin protein arrays on metallic nanostructures | 期刊論文 |
31 | Graphene as a conductive additive to enhance the high-rate capabilities of electrospun Li4Ti5O12 for lithium-ionbatteries | 期刊論文 |
32 | Aptamer biosensor for protein detection using gold nanoparticles | 期刊論文 |
33 | A Simple and Sensitive Biosensor Based on Silver Enhancement of Aptamer-Gold Nanoparticle Aggregation | 期刊論文 |
34 | 時間分辨拉曼光譜研究一氧化氮與肌紅蛋白的結合過程 | 期刊論文 |
35 | Ultrafast Photoinduced Electron Transfer between Tetramethylrhodamine and Guanosine in Aqueous Solution | 期刊論文 |
36 | Transfer printing of metal nanoparticles with controllable dimensions, placement and reproducible SERS effects | 期刊論文 |
37 | Thermo-chemically patterning of polymer film with tunable size reductions using metal-coated PDMS stamps | 期刊論文 |
38 | Probing Protein Functions via Optical Single Molecule Detection | 期刊論文 |
39 | Maximum Entropy Method for Analyses of Fluorescence Correlation Spectra of Oligonucleotide Intra-Chain Collision | 期刊論文 |
40 | Integrated Microfluidic Chips. | 期刊論文 |
41 | Fluorescence quenching of TMR by guanosine in oligonucleotides | 期刊論文 |
42 | Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides | 期刊論文 |
43 | Aggregation Behaviors of Tricarbocyanine Dye in water and in AOT Reverse Micelles | 期刊論文 |
44 | Micro/nano patterning of phenolic resin using hot microcontact printing | 期刊論文 |
45 | A Microfluidic Mixer for Single-Molecule Kinetics Experiments | 期刊論文 |
46 | Thinner is better: ultrathin conducting oligoaniline film for gas microsensors with ultralow detection limit | 期刊論文 |
47 | Temperature Dependence of Interaction between Double Stranded DNA and Cy3 or Cy5 | 期刊論文 |
48 | 中級物理化學 | 學術專著 |
49 | Structural Heterogeneity in the Collision Complex between Organic Dyes and Tryptophan in Aqueous Solution | 期刊論文 |
50 | A chip-to-chip nanoliter microfluidic dispenser | 期刊論文 |
51 | Subdomain-specific collapse of denatured staphylococcal nuclease revealed by single molecule FRET measurements | 期刊論文 |
項目摘要
為了實現高通量、高特異性和高靈敏度的生物分子檢測，我們提出發展一類集新型分子識別技術、超精細結構非傳統加工、集成微流晶片實驗室和現代光譜學與成像技術於一體的新方法。這一方法將電化學分子識別技術與現代雷射光譜學和紅外成像技術結合，兩種手段互相補充用於進一步提高識別的特異性和檢測的準確度。通過納米尺度的超精細結構製備與微米尺度的大規模集成微流晶片技術相結合，採取非傳統的微納加工方法，提高微流晶片的檢測通量和靈敏度，並實現操作和檢測的全自動化。結合集成晶片和光學成像等高通量手段和電化學、光譜學的高靈敏度檢測方法，在有限的空間和時間內對微量樣品進行更加全面的分析，突破傳統技術的在通量、精度上的局限和對樣品量的依賴。
