基本介紹
- 中文名：生物核磁共振實驗室
- 外文名：BioNMRLab
基本信息,成員簡介,施蘊渝,吳季輝,張志勇,龔慶國,阮科,研究方向,表觀遺傳結構生物學研究,非編碼RNA的結構生物學研究,生物大分子核磁共振方法研究,基於片段的小分子化合物篩選,畢業生去向,代表性論文,2019,2018,2017,2016,2015,2014,2013,2012,2011,2010,2009,2008,2007,2006,2005,2001,
基本信息
BioNMRLab隸屬於中國科學技術大學生命科學學院和合肥微尺度物質科學國家實驗室，地址位於安徽省合肥市蜀山區黃山路445號，中國科學技術大學生命科學院。實驗室現有（截至2019年9月4日）中國科學院院士1名，正教授（不含院士）3名，副教授2名，講師、管理員、工程師、實驗員等6名，博士後3名，博士、碩士研究生30餘名。實驗室成立近30年間，培養了十餘名教授、二十餘名副教授、上百名碩士博士生等科研界人才，以及眾多行政領域、商業領域人才。
成員簡介
施蘊渝
研究方向：蛋白質分子設計及藥物設計有關的基礎理論和方法學的研究，包括酶與底物，藥物與靶標分子結合自由能的計算，蛋白質穩定性的計算機模擬，蛋白質分子的隨機動力學模擬，蛋白質靜電相互作用研究，以及酶作用機理的計算機模擬。生物大分子蛋白質及核酸的晶體學、核磁共振波譜學結構與功能機理研究。
吳季輝
研究方向：蛋白質和核酸的核磁共振研究及生物核磁共振波譜學方法學研究，特別是蛋白質的結構解析及結構和功能關係，以及蛋白質-蛋白質，蛋白質-核酸，蛋白質-配基相互作用,結構基因組學研究中適於高通量結構測定以及代謝組學中的核磁共振方法學研究。
張志勇
研究方向：多尺度模擬方法和實驗技術（例如核磁共振和小角度X射線散射等）相結合研究生物大分子的結構與功能。
龔慶國
阮科
研究方向
實驗室主要套用核磁共振波譜學(NMR)綜合X-射線晶體學衍射，冷凍電鏡，生物分子生物學和細胞生物學等方法來研究生物大分子結構和動力學,蛋白質-蛋白質相互作用,蛋白質-DNA/RNA相互作用和蛋白質-配體相互作用, 來解析生命過程中重要的生物大分子及其複合物的功能。
表觀遺傳結構生物學研究
非編碼RNA的結構生物學研究
從轉運RNA（tRNA）和核糖體RNA（rRNA）到snoRNA、microRNA、siRNA、snRNA、exRNA、piRNA、和lncRNA等，非編碼RNA是一類不會翻譯成蛋白質但卻具有重要生物學功能的RNA分子，在體內廣泛存在。最新的測序研究證實，人類基因組中約有98%的部分是不編碼蛋白的基因，而只一點與其他物種相比時有著鮮明的區別，一定程度上解釋了為什麼哺乳動物遠比其他物種複雜。
然而儘管非編碼RNA已被發現在包括基因表達調控和剪下等細胞內過程中，以及癌症等疾病的發生髮展中扮演重要角色，大量非編碼RNA的精確的功能意義以及它們的結構功能關係仍然很大程度上未知。目前的研究發現，RNA分子在結構和功能上存在很大的靈活性。另外，由於RNA分子帶有強負電，不易於結晶，使得使用X-射線晶體衍射方法對其進行結構研究變得困難。因此使用包括溶液核磁共振、小角散射、和冷凍電鏡等在內的其他結構生物學手段研究RNA結構變得極為重要。其中核磁共振在研究非編碼RNA這類結構上存在高度靈活性的生物分子時具有一定的優勢。
實驗室目前主要使用核磁共振和X-射線晶體衍射方法研究大腸桿菌里的非編碼RNA以及它們與RNA分子伴侶蛋白Hfq複合物的結構。我們希望通過這些研究能進一步揭示非編碼RNA在應激條件下調控下游mRNA翻譯表達的分子機制。
生物大分子核磁共振方法研究
X-射線晶體學，核磁共振波譜學和冷凍電鏡是目前解析高分辨生物大分子三維結構的主要方法。其它方法，包括X-射線小角散射，電子順磁共振（EPR），PCS和順磁弛豫增強（PRE）等方法提供了重要的補充信息.我們套用和發展上述多種結構解析方法，使用多種解析度下的實驗室數據和分子動力學建模來研究生物大分子在多種時間尺度下的結構和動力學性質。
基於片段的小分子化合物篩選
靶標蛋白-蛋白相互作用體系被譽為藥物發現的明珠。選擇性的阻滯特定蛋白-蛋白相互作用極大地擴充了現有的藥物靶點。蛋白-蛋白相互作用的熱點區域相比於經典的激酶口袋，更為狹窄舒緩，因此發現高親和力、高選擇性的小分子抑制劑面臨著巨大的挑戰。2000年以來興起的基於片段的先導化合物發現在新型的靶點研究上成果豐碩，目前已有一個藥物上市，數十種藥物進入臨床。我們建立了國內首批高度自動化的核磁片段篩選平台，針對和腫瘤增殖和遷移密切關聯的表觀遺傳靶點和小GTP酶體系，發現了這類靶點的弱結合的苗頭化合物，並在結構指導下拼裝為類似先導化合物的化學探針。探索這類探針在細胞內阻滯相應的蛋白-蛋白互作，從而抑制腫瘤增殖和遷移的分子機制。
畢業生去向
| 序號 | 姓名 | 畢業論文題目 | 畢業時間 | 畢業去向 | 所獲學位 |
|---|---|---|---|---|---|
84 | 鮑洪宇 | 人源蛋白質ARAP3選擇性識別RhoA的結構基礎以及擬南芥蛋白質APUM23特異性識別18S核糖體RNA的結構基礎 | 2017 | 南方科技大學 | 博士 |
83 | 江依洋 | 肺炎鏈球菌核糖體23S RNA 甲基轉移酶RlmCD結構與功能研究 | 2017 | 中國科學技術大學 | 博士 |
82 | 彭俊輝 | 生物大分子的整合結構模擬新方法的研究 | 2017 | 香港科技大學 | 博士 |
81 | 孫愛愛 | 人源PRC2複合物的游離組分AEBP2和PHF1的結構與功能研究 | 2017 | 浙江大學 | 博士 |
80 | 汪娜 | SWI/SNF中bromodomain的小分子抑制劑的鑑定和複合物結構研究 | 2017 | 南方科技大學 | 博士 |
79 | 徐玲 | 大腸桿菌二聚體DEAD-box RNA解旋酶結構與功能的研究 | 2017 | 博士 | |
78 | 陳昌寧 | 稀疏採樣與FLYA算法結合進行蛋白質核磁譜圖的快速指認 | 2016 | 碩士 | |
77 | 董俊 | 計算機模擬研究表皮生長因子受體的pH調控及CR4/5-TRBD複合物的結合過程 | 2016 | 上海京頤科技 | 碩士 |
76 | 高佳 | LARG/RhoA複合物及BRM蛋白的小分子抑制劑結構與功能研究 | 2016 | 合肥物質科學院醫學物理與技術中心 | 博士 |
75 | 呂宇 | 鐵離子飢餓下小RNA分子RyhB對大腸桿菌細胞生理和代謝影響的研究 | 2016 | 博士 | |
74 | 馬榮聲 | 利用核磁共振觀測激發態的贗接觸位移 | 2016 | 中國科學技術大學 | 博士 |
73 | 文彬 | 結合實驗和計算的方法來闡述 柔性蛋白質的結構和動力學 | 2016 | 博士 | |
72 | 吳鵬志 | 大腸桿菌非編碼小RNA DsrA核磁結構與功能研究 | 2016 | 博士 | |
71 | 徐莉 | 人源CBP Bromodomain 與乙醯化組蛋白H3K56的結構及功能研究 | 2016 | 北京大學 | 碩士 |
70 | 朱姍姍 | 人源UBF 蛋白質結構與功能研究 | 2016 | 碩士 | |
69 | 鮑雲 | 人源PHF6 extended PHD1的結構和功能研究 | 2015 | 中科院植物逆境生物學研究中心 | 碩士 |
68 | 陳旭文 | 人源TRIM66的PHD-Bromo串聯模組的結構與功能研究 | 2015 | 碩士 | |
67 | 李賽 | Galectin-8C-CRD識別NDP52368-381的結構與功能研究及核小體的核磁方法初步研究 | 2015 | 美國Rockefeller大學 | 博士 |
66 | 劉鐘華 | Structure and Function of the PHF6 extended PHD2 domain&PHF6 and RBBP4 interaction | 2015 | 美國Case western reserve University | 博士 |
65 | 王軍成 | 人源BS69 蛋白質Bromo-ZnF-PWWP 模組的結構與功能研究 | 2015 | 美國Memorial Sloan-Kettering Cancer Center | 博士 |
64 | 王麗君 | 大腸桿菌RNA分子伴侶Hfq以及RNA解旋酶CsdA的結構與功能研究 | 2015 | 上海蛋白質中心 | 博士 |
63 | 余曦 | 腫瘤細胞中Jhdm1b代謝調控功能的系統生物學研究 | 2015 | 博士 | |
62 | 張泳輝 | 計算機模擬整合X射線小角散射實驗數據研究蛋白質的溶液構象 | 2015 | 香港城市大學 | 碩士 |
61 | 鮑小玲 | 核磁共振研究hMog1和Ran蛋白質複合物的結構與功能 | 2014 | 中國科學技術大學 | 博士 |
60 | 劉恆 | 人的Mog1/Ran複合物在有絲分裂中的功能研究及Mog1/Ran/RanBP1 複合物結構和功能的初步研究 | 2014 | 美國University of Pittsburgh | 博士 |
59 | 邵振華 | 組蛋白磷酸化與tRNA甲基化相關蛋白的結構與功能研究 | 2014 | 美國University of Texas Southwestern Medical Center | 博士 |
58 | 王崇元 | 布魯斯錐蟲精氨酸甲基轉移酶6和7的結構與酶活研究 | 2014 | 美國Memorial Sloan-Kettering Cancer Center | 博士 |
57 | 趙德彪 | 人源蛋白CAP的串聯SH3結構域與細胞骨架蛋白vinculin相互作用的結構生物學研究 | 2014 | 美國Mayo Clinic | 博士 |
56 | 鄒楊 | 人ECT2蛋白N端結構域的結構以及RNA核磁方法的研究 | 2014 | GE公司 | 博士 |
55 | 唐雅珺 | 酵母氧化應激蛋白質Grx8的核磁結構和酶催化機制研究 | 2014 | 中國科學技術大學 | 博士 |
54 | 車影 | 人源賴氨酸去甲基化酶KDM5B的結構與功能研究 | 2013 | 碩士 | |
53 | 龔德順 | 人源 RBM25蛋白的 PWI結構域及其鄰近鹼性區域的結構與功能研究 | 2013 | 清華大學 | 博士 |
52 | 李福東 | 人源 NSD 家族蛋白質的 PHD5-C5HCH模組的結構與功能研究 | 2013 | 中國科學技術大學 | 博士 |
51 | 何超 | 裂殖酵母蛋白Stc1及人源NSD3的PHD5-C5HCH結構域的結構與功能研究 | 2012 | 安徽大學 | 博士 |
50 | 劉凱先 | 核磁方法研究人源 Mog1-Ran的結合界面 | 2012 | 美國University of Maryland | 碩士 |
49 | 劉磊 | 人源 BRPF2 第二個 PHD 鋅指結構域的結構和功能和人源組蛋白乙醯轉移酶 MOZ 的活性研究 | 2012 | 諾華公司 | 博士 |
48 | 聞捷 | 快速四維NMR技術在天然無序蛋白質主鏈化學位移指認和甲基-甲基NOESY實驗中的套用 | 2012 | 美國Washington University in St. Louis | 博士 |
47 | 張紹傑 | Vt結構域與LD motif的相互作用，人的Spt6串聯SH2結構以及Skip與PPIL1對剪下的影響 | 2012 | 博士 | |
46 | 鄧忠牧 | 人BAF155 SWIRM結構域和BAF47 SNF5結構域的結構功能和相互作用研究 | 2011 | 碩士 | |
45 | 紀鵬 | 人類 ZO-1 蛋白第二個 PDZ 結構域的溶液結構與功能 | 2011 | 美國Vertafore軟體公司 | 博士 |
44 | 李鑫鑫 | 釀酒酵母蛋白Rtt107的C端串聯BRCT結構域的結構和功能研究 | 2011 | Simcere製藥公司 | 博士 |
43 | 秦蘇 | 人BRPF2和BS69蛋白的幾個結構域的結構與功能研究 | 2011 | 南方科技大學 | 博士 |
42 | 邱宇 | 酵母賴氨酸甲基轉移酶Set9調控蛋白Pdp1的PWWP結構域的結構生物學研究 | 2011 | 美國St. Jude Children's Research Hospital/美國Sanofi製藥公司 | 博士 |
41 | 芮斌 | 大腸桿菌JM101在氧化應激狀態下代謝流量分配的內在機制 | 2011 | 安徽農業大學 | 博士 |
40 | 宋震偉 | RBM5的RRM2結構功能研究HP1和Borealin相互作用的核磁研究 | 2011 | 美國University of Missouri | 博士 |
39 | 湯衡 | ARAP3、SmD-SmD2、SPF30蛋白質結構的研究 | 2011 | 合肥安為康醫學檢驗所 | 碩士 |
38 | 王維維 | 大腸桿菌RNA分子伴侶Hfq的結構以及與RNA相互作用的研究 | 2011 | 美國Rockefeller大學 | 博士 |
37 | 黃小娟 | 剪下因子 FBP21 雙 WW 結構域的溶液結構和生物學功能研究 | 2010 | 美國Cornell大學 | 博士 |
36 | 黃鴻達 | 組蛋白分子伴侶 Rtt106 以及人的Brd2 蛋白結構與生物學功能研究 | 2010 | 美國Memorial Sloan-Kettering Cancer Center/南方科技大學 | 博士 |
35 | 劉建平 | 轉錄因子 Spt6 的串聯 SH2 結構域識別 RNA 聚合酶 II C-端結構域磷酸化修飾的結構生物學研究 | 2010 | 上海植物生理研究所 | 博士 |
34 | 路國偉 | RGS12 PDZ domain和MEK2之間pH依賴相互作用的分子機理研究 | 2010 | 博士 | |
33 | 申鐵 | 13C代謝通量分析平台的建立和改良 | 2010 | 貴州師範大學 | 博士 |
32 | 吳勃 | 人源蛋白Vav2和Arap3的結構與功能研究 | 2010 | 合肥物質科學院強磁場中心 | 博士 |
31 | 吳芳明 | 釀酒酵母蛋白Prp20p以及人源鋅指蛋白DESR1的結構與功能研究 | 2010 | 合肥物質科學院強磁場中心 | 博士 |
30 | 吳清林 | UBXD2 UBX 結構域結構與功能研究和核磁共振方法研究樹枝形分子與生物表面活性劑相互作用及基於樹枝形分子的藥物篩選 | 2010 | 美國Duke University | 博士 |
29 | 洪靖君 | ScPDCD5 蛋白溶液結構、主鏈動力學及其功能研究 | 2009/td> | 美國NIH | 博士 |
28 | 劉志軍 | 液體核磁共振及生物光譜學方法研究hUBF HMG BOX-5 蛋白質體外摺疊,穩定性,及熱力學中間態系綜 | 2009 | 上海蛋白質中心 | 博士 |
27 | 程義雲 | 基於樹枝形分子的藥物運輸系統：從理論到套用 | 2008 | 華東師範大學 | 博士 |
26 | 胡琦 | MAPKs激酶及PB1結構域在信號傳遞中的作用 | 2008 | 美國Mayo Clinic | 博士 |
25 | 劉將新 | LARG PDZ 結構域在自由和複合物狀態下結構與動力學的研究 | 2008 | 中科院昆明植物所 | 博士 |
24 | 劉迎 | hBrd4 BD2和hMog1溶液結構與功能的研究 | 2008 | 博士 | |
23 | 陳泉 | NMR 方法對蛋白複合物結構的研究 | 2007 | 中國科學技術大學 | 博士 |
22 | 金先菊 | MICAL-1 CH 結構域的摺疊和結構穩定性的初步研究 | 2007 | 碩士 | |
21 | 牛曉剛 | 利用核磁共振方法研究蛋白質與小肽相互作用時的毫秒動力學行為 | 2007 | 北京大學/北京核磁共振中心 | 博士 |
20 | 孫紅賓 | 殘留的偶極耦合在生物大分子溶液結構研究中的套用及人的MICAL-1 蛋白的 Calponin Homology 結構域和 BRD7 蛋白的Bromodomain 結構域的結構與功能研究 | 2007 | 合肥物質科學院強磁場中心 | 博士 |
19 | 沈為群 | "人Brg1 Bromodomain結構域的溶液結構及其與乙醯化組蛋白的相互作用研究" | 2007 | 博士 | |
18 | 史喆 | 分子對接與核磁共振方法篩選人雙特異性磷酸酶VHR抑制劑的研究 | 2007 | 益方生物科技公司 | 博士 |
17 | 吳家文 | 人類ZO2蛋白質第二個PDZ結構域的溶液結構和功能的研究 | 2007 | 安徽中醫藥大學 | 博士 |
16 | 徐珺劼 | 類泛素修飾蛋白 Saccharomyces cerevisae Urm1 的溶液結構測定、進化意義分析及功能研究 | 2007 | 美國University of Texas Southwestern Medical Center | 博士 |
15 | 戴海明 | 人Coactosin Like Protein及MICAL_1的Calponin Homolgy結構域的結構與功能研究 | 2006 | 美國Mayo Clinic/合肥物質科學院醫學物理與技術中心 | 博士 |
14 | 丁虎生 | 人泛素類相關修飾蛋白質SUMO-3 C47S的溶液結構測定及其與SUMO化路徑中的綴合酶Ubc9的相互作用研究 | 2006 | 美國Mayo Clinic | 博士 |
13 | 李祥 | 人GOPC蛋白PDZ結構域溶液結構的測定及其與Neuroligin和Frizzled 8 C-末端多肽相互作用的研究 | 2006 | 美國 | 博士 |
12 | 王丹丹 | hUBF HMG box 5蛋白摺疊和結構穩定性的初步研究 | 2006 | 碩士 | |
11 | 孫建萍 | 酵母蛋白質Ktillp的溶液構象研究及人的蛋白質HSPC159的保守結構域的克隆、表達、純化和初步晶體學研究 | 2005 | 博士 | |
10 | 徐超 | 人的PPIL1 蛋白質溶液結構入功能研究和SH3BGRL3蛋白質的溶液結構及細胞功能研究 | 2005 | 中國科學技術大學 | 博士 |
9 | 姚波 | 蛋白CMS SH3結構域溶液結構的測定及其與c-Cbl C-末端多肽相互作用的研究 | 2005 | 美國University of Texas Southwestern Medical Center | 碩士 |
8 | 張學成 | 酸誘導變性態hUBF HMG Box1的初步研究 | 2005 | 安徽大學 | 博士 |
7 | 周賀鉞 | AF-6 PDZ結構域的溶液結構測定及其與Neurexin和Bcr C-末端多肽的相互作用研究 | 2005 | 博士 | |
6 | 蔡征 | 蠍毒素BmP02和人上游結合因子hUBF的HMG Box 1的溶液構象測定 | 2001 | 博士 | |
5 | 曾王勇 | 短鏈蠍毒素BmBKTx1、BmKX和BmK38的溶液構象測定 | 2004 | 博士 | |
4 | 張志勇 | Computer Simulations for Folding/Unfolding of Peptides and Large-scale Functional Motions of Proteins | 2003 | 中國科學技術大學 | 博士 |
3 | 賀建斌 | 有效地搜尋蛋白質構象 | 2003 | 碩士 | |
2 | 楊武林 | 人的上游結合因子hUBF中的兩個HMG box結構域：hUBF HMG box 1和hUBF HMG box 5的克隆、表達純化以及結構和功能的比較研究 | 2002 | 合肥物質科學院醫學物理與技術中心 | 博士 |
1 | 徐應琪 | 蠍毒素BmP02和人上游結合因子hUBF的HMG Box 1的溶液構象測定 | 2001 | 英國帝國理工大學 | 博士 |
代表性論文
2019
Guodong Xie*, Tommy V. Vo*, Gobi Thillainadesan, …, Yunyu Shi, Fudong Li# & Shiv I. S. Grewal#A conserved dimer interface connects ERH and YTH family proteins to promote gene silencingNature communications(2019) 10(1):251.[Full Text].
Lin Cheng, Fudong Li, Yiyang Jiang, Hailong Yu, Changlin Xie, Yunyu Shi#, Qingguo Gong#.Structural insights into a unique preference for 3′ terminal guanine of mirtron in Drosophila TUTase tailor.Nucleic Acids Rsearch.(2019) 47(1):495-508.[Full Text].
Mengqi Lv*, Yixiang Yao*, Fudong Li, Ling Xu, … ,Yunyu Shi, Yujie Fan#, Yajun Tang#.Structural insights reveal the specific recognition of roX RNA by the dsRNA-binding domains of the RNA helicase MLE and its indispensable role in dosage compensation in Drosophila.Nucleic Acids Rsearch.(2019) 47(6):3142-3457.[Full Text].
Xiaodan Liu, Shengqi Shen, Pengzhi Wu, Fudong Li, … ,Xuebiao Yao#, Huafeng Zhang#, Yunyu Shi#.Structural insights into dimethylation of 12S rRNA by TFB1M: indispensable role in translation of mitochondrial genes and mitochondrial function.Nucleic Acids Rsearch.(2019) gkz505[Full Text].
Junhui Peng*, Chuang Yuan*, Rongsheng Ma, Zhiyong Zhang#.Backmapping from Multiresolution Coarse-Grained Models to Atomic Structures of Large Biomolecules by Restrained Molecular Dynamics Simulations Using Bayesian Inference.J. Chem. Theory Computation.(2019) 15(5):3344-3353.[Full Text].
2018
Yaqing Zhao, Guang Zhang, Chao He, Yide Mei, Yunyu Shi# and Fudong Li#.The 11th C2H2 zinc finger and an adjacent C-terminal arm are responsible for TZAP recognition of telomeric DNA.Cell Research(2018) 28:130-134.[Full Text].
Aiai Sun, Fudong Li, Zhonghua Liu, Yiyang Jiang, Jiahai Zhang, Jihui Wu, Yunyu Shi#.Structural and biochemical insights into human zinc finger protein AEBP2 reveals interactions with RBBP4.Protein Cell(2018) 9(8):738-742.[Full Text].
Yiyang Jiang*, Hailong Yu*, Fudong Li, Lin Cheng, Lingru Zhu, Yunyu Shi# , Qingguo Gong#.Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD.PloS Pathogens.(2018) 14(11):e1007379.[Full Text].
Difei Xu, Rongsheng Ma, Jiahai Zhang, ... , Yunyu Shi, Jihui Wu, Qiang Wu, Zhiyong Zhang#, Ke Ruan#.Dynamic Nature of CTCF Tandem 11 Zinc Fingers in Multivalent Recognition of DNA As Revealed by NMR Spectroscopy.J. Phys. Chem Lett.(2018) 9(14):4020-4028.[Full Text].
Difei Xu*, Bin Li*, Jia Gao, Zhijun Liu, Xiaogang, ... , Rongsheng Ma#, Daiwen Yang#, Ke Ruan#.Ligand Proton Pseudocontact Shifts Determined from Paramagnetic Relaxation Dispersion in the Limit of NMR Intermediate Exchange.J. Phys. Chem Lett.(2018) 9(12):3361-3367.[Full Text].
Xiaoling Bao, Heng Liu, Xing Liu, … , Jihui Wu#, Yunyu Shi#.Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation.J. Mol. Cell Biol.(2018) 10(1):18-32.[Full Text].
2017
Xiaoling Bao, Heng Liu, Xing Liu,..., Xuebiao Yao#, Jihui Wu#, Yunyu Shi#Mitosis-specific acetylation tunes Ran effector binding for chromosome segregationJ. Mol. Cell Biol.(2017)[Full Text].
Bin Wen, Weiwei Wang, Jiahai Zhang, Qingguo Gong, Yunyu Shi, Jihui Wu# and Zhiyong Zhang#.Structural and dynamic properties of the C-terminal region of the Escherichia coli RNA chaperone Hfq: integrative experimental and computational studies.Phys. Chem. Chem. Phys.(2017) 19:21152-21164.[Full Text].
Yiyang Jiang, Fudong Li, Jihui Wu, Yunyu Shi, Qingguo Gong#Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCDPlos One(2017)[Full Text].
Hongyu Bao, Na Wang, Chongyuan Wang,..., Jihui Wu#, Yunyu Shi#Structural basis for the specific recognition of 18S rRNA by APUM23Nucleic Acids Research(2017) 45(20):12005-12014[Full Text].
Lingna Yang, Chongyuan Wang, Fudong Li,...,Jihui Wu, Yunyu Shi, Qingguo Gong#The human RNA-binding protein and E3 ligase MEX-3C binds the MEX-3–recognition element (MRE) motif with high affinity.J. Biol. Chem.(2017) 292:16221-16234[Full Text].
Aiai Sun, Fudong Li, Zhonghua Liu, Yiyang Jiang, Jiahai Zhang, Jihui Wu#, Yunyu Shi#Structural and biochemical insights into human zinc finger protein AEBP2 reveals interactions with RBBP4Protein&Cell(2017).[Full Text].
Ling Xu, Lijun Wang, Junhui Peng,...,Jihui Wu, Yajun Tang#, Yunyu Shi#Insights into the Structure of Dimeric RNA Helicase CsdA and Indispensable Role of Its C-Terminal Regions.Structure(2017) 25(12):1795-1808[Full Text].
Pengzhi Wu, Xiaodan Liu, Lingna Yang, Yitong Sun, Qingguo Gong, Jihui Wu#, Yunyu Shi#The important conformational plasticity of DsrA sRNA for adapting multiple target regulation .Nucleic Acids Research(2017) 45(16):9625-9639[Full Text].
Mengqi Lv, Chongyuan Wang, Fudong Li, Junhui Peng, Qingguo Gong, Yunyu Shi, Yajun Tang#.Structural insights into the recognition of phosphorylated FUNDC1 by LC3B in mitophagy.Protein&Cell(2017) 8(25):25-38.[Full Text].
Li Xu*, Aimin Cheng*, Min Huang,..., Jihui Wu, Catherine C. L., Ke Ruan#.Structural insight into the recognition of acetylated histone H3K56ac mediated by the bromodomain of CREB-binding protein.The FEBS journal(2017) 284(20):3422-3436.[Full Text].
Jia Gao, E. Liang, Rongsheng Ma,..., Jihui Wu, Xuncheng Su, Wei He, Ke Ruan#Fluorine Pseudocontact Shifts Used for Characterizing the Protein–Ligand Interaction Mode in the Limit of NMR Intermediate ExchangeAngew. Chemie Int. Ed.(2017) 129(42):13162-13166[Full Text].
J Liu, F Li, H Bao, Y Jiang, S Zhang, R Ma, J Gao, J Wu, K Ruan#.The polar warhead of a TRIM24 bromodomain inhibitor rearranges a water‐mediated interaction network.The FEBS journal(2017) 284(7):1082-1095.[Full Text].
J Liu, J Gao, F Li, R Ma, Q Wei, A Wang, J Wu, K Ruan#.NMR characterization of weak interactions between RhoGDI2 and fragment screening hits.BBA-General Subjects(2017) 1861(1):3061-3070.[Full Text].
2016
Qingtao Wei, Jiajing Chen, Juan Mi, Jiahai Zhang, Ke Ruan# and Jihui Wu#.NMR Backbone Assignment of Large Proteins by Using (13) Cα -Only Triple-Resonance Experiments.Chemistry-A European Journal(2016) 22(28):9556-9564.[Full Text].
Na Wang, Fudong Li, Hongyu Bao, Jie Li, Jihui Wu# and Ke Ruan#.NMR Fragment Screening Hit Induces Plasticity of BRD7/9 Bromodomains.ChemBioChem(2016) 17(15):1456-1463.[Full Text].
Rongsheng Ma, Pengchao Wang, Jihui Wu and Ke Ruan#.Process of Fragment-Based Lead Discovery—A Perspective from NMR.Molecules(2016) 21:854.[Full Text].
Rongsheng Ma, Q.F.Li, Aidong Wang, Jiahai Zhang, Zhijun Liu,Jihui Wu Xuncheng Su# and Ke Ruan#.Determination of pseudocontact shifts of low-populated excited states by NMR chemical exchange saturation transfer.Phys. Chem. Chem. Phys.(2016) 18:13794-13798.[Full Text].
Hongyu Bao, Fudong Li, Chongyuan Wang, Na Wang, Yiyang Jiang, Yajun Tang, Jihui Wu#, and Yunyu Shi#.Structural Basis for the Specific Recognition of RhoA by the Dual GTPase-activating Protein ARAP3.J. Biol. Chem.(2016) 291(32):16709-16719.[Full Text].
Junhui Peng, and Zhiyong Zhang#.Unraveling low-resolution structural data of large biomolecules by constructing atomic models with experiment-targeted parallel cascade selection simulations.Sci. Rep.(2016) 6:29360.[Full Text].
Jun Dong*, Yonghui Zhang*, Zhiyong Zhang#.pH dependence of ligand-induced human epidermal growth factor receptor activation investigated by molecular dynamics simulations.Journal of Molecular Modeling.(2016) 22(6):1-12.[Full Text].
Yun Bao, Zhonghua Liu, Jiahai Zhang, Jihui Wu#, and Yunyu Shi#.1H, 13C and 15N resonance assignments and secondary structure of the human PHF6-ePHD1 domain.Biomolecular NMR Assignment.(2016) 10(1):1-4.[Full Text].
Chongyuan Wang, Yuwei Zhu, Hongyu Bao, Yiyang Jiang, Chao Xu, Jihui Wu#, and Yunyu Shi#.A novel RNA-binding mode of the YTH domain reveals the mechanism for recognition of determinant of selective removal by Mmi1.Nucleic Acids Research(2016) 44(2):968-982.[Full Text].
2015
Yonghui Zhang, Bin Wen, Junhui Peng, Xiaobing Zuo, Qingguo Gong and Zhiyong Zhang#.Determining structural ensembles of flexible multi-domain proteins using small-angle X-ray scattering and molecular dynamics simulations.Protein&Cell(2015) 6(8):619-623.[Full Text].
Lijun Wang, Weiwei Wang, Fudong Li, Jiahai Zhang, Jihui Wu, Qingguo Gong# and Yunyu Shi#.Structural insights into the recognition of the internal A-rich linker from OxyS sRNA by Escherichia coli Hfq.Nucleic Acids Research(2015) 43(4):2400-2411.
Zhonghua Liu, Fudong Li, Beibei Zhang, Sai Li, Jihui Wu# and Yunyu Shi#.Structural Basis of Plant Homeodomain Finger 6 (PHF6) Recognition by Retinoblastoma Binding Protein 4 (RBBP4) Component of the Nucleosome Remodeling and Deacetylase (NuRD) Complex.Journal of Biological Chemistry(2015) 290:6630-6638.
2014
Fudong Li*, Debiao Zhao*, Jihui Wu# and Yunyu Shi#.Structure of the YTH domain of human YTHDF2 in complex with an m6A mononucleotide reveals an aromatic cage for m6A recognition.Cell Research(2014) 24(12):1490-1492.
Juncheng Wang, Su Qin, Fudong Li, Sai Li, Wei Zhang, Junhui Peng, Zhiyong Zhang, Qingguo Gong, Jihui Wu#, Yunyu Shi#.Crystal structure of human BS69 Bromo-ZnF-PWWP reveals its role in H3K36me3 nucleosome binding.Cell Research(2014) 24:890–893.
Bin Wen, Junhui Peng, Xiaobing Zuo, Qingguo Gong, Zhiyong Zhang#.Characterization of Protein Flexibility Using Small-Angle X-Ray Scattering and Amplified Collective Motion SimulationsBiophysical Journal(2014)4(19):956-964.[Full Text].
Zhenhua Shao*, Wei Yan*, Junhui Peng, Xiaobing Zuo, Yang Zou, Fudong Li, Deshun Gong, Rongsheng Ma, Jihui Wu, Yunyu Shi, Zhiyong Zhang, Maikun Teng,Xu Li#, and Qingguo Gong#.Crystal structure of tRNA m1G9 methyltransferase Trm10: insight into the catalytic mechanism and recognition of tRNA substrate.Nucleic Acids Research2014, Vol. 42, No. 1 509-525>.
Jia Gao,Rongsheng Ma,Wei Wang,Na Wang,Ryan Sasaki,David Snyderman,Jihui Wu,Ke Ruan.Automated NMR Fragment Based Screening Identified a Novel Interface Blocker to the LARG/RhoA Complex.PLOS ONE(2014)9(2): e88098.
Chongyuan Wang, Yuwei Zhu, Tamar B Caceres, Lei Liu, Junhui Peng, Junchen Wang, Jiajing Chen, Xuwen Chen, Zhiyong Zhang, Xiaobing Zuo, Qingguo Gong, Maikun Teng, Joan M Hevel, Jihui Wu#and Yunyu Shi#.Structural Determinants for the Strict Monomethylation Activity by Trypanosoma bruceiProtein Arginine Methyltransferase 7.Structure. 2014 May 6; 22(5): 756-768
Chongyuan Wang*,Yuwei Zhu*,Jiajia Chen,XuLi,Junhui Peng,Jiajing Chen,Yang Zou,Zhiyong Zhang,Hong Jin,Pengyuan Yang,Jihui Wu,Liwen Niu,Qingguo Gong,Maikun Teng#,Yunyu Shi#.Crystal structure of arginine methyltransferase 6 from Trypanosoma bruceiPLoS One2014 Feb 3; 9(2): e87267
Zhonghua Liu, Fudong Li, Ke Ruan, Jiahai Zhang, Yide Mei, Jihui Wu# and Yunyu Shi#.Structural and Functional Insights into the Human Borjeson-Forssman-Lehmann Syndrome-associated Protein PHF6.J Biol Chem, 2014, April 4; 289(14):10069-83
2013
Wang Weiwei*, Wang Lijun*, Wu Jihui, Gong Qingguo#, Shi Yunyu#.Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.Nucleic Acids Res. 2013 Jun 1; 41(11): 5938-48.
Chao He, Sreerekha S. Pillai*, Francesca Taglini*, Fudong Li, Ke Ruan, Jiahai Zhang, Jihui Wu, Yunyu Shi#, ElizabethH. Bayneb#.Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification.Proc Natl Acad Sci U S A. 2013 May 21;110(21):E1879-88.
Chao He*, Fudong Li*, Jiahai Zhang, Jihui Wu#, and Yunyu Shi#.The Methyltransferase NSD3 Has Chromatin-binding Motifs, PHD5-C5HCH, That Are Distinct from Other NSD (Nuclear Receptor SET Domain) Family Members in Their Histone H3 Recognition.J Biol Chem. 2013 Feb 15;288(7):4692-703.
Deshun Gong, Fan Yang, Fudong Li, Dandan Qian, Minhao Wu, Zhenhua Shao, Mian Wu, Jihui Wu#, Yunyu Shi#.Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region.Biochem J. 2013 February 15; 450(Pt 1): 85–94.
Sai Li*, M. P. Wandel*, Fudong Li, Zhonghua Liu, Chao He, Jihui Wu, Yunyu Shi#, F. Randow#.Sterical Hindrance Promotes Selectivity of the Autophagy Cargo Receptor NDP52 for the Danger Receptor Galectin-8 in Antibacterial Autophagy.Science Signal. 2013 Feb 5;6(261):ra9.
2012
Yu Qiu, Lei Liu, Chen Zhao, Chuanchun Han, Fudong Li, Jiahai Zhang, Yan Wang, Guohong Li, Yide Mei, Mian Wu, Jihui Wu#, Yunyu Shi#.Combinatorial readout of unmodified H3R2 and acetylated H3K14 by the tandem PHD finger of MOZ reveals a regulatory mechanism for HOXA9 transcription.Genes & Dev. 2012. 26: 1376-1391.
Zhenwei Song, Peiwen Wu, Peng Ji, Jiahai Zhang, Qingguo Gong, Jihui Wu#, Yunyu Shi#.Solution structure of the second RRM domain of RBM5 and its unusual binding characters for different RNA targets.Biochemistry.2012 Aug 21;51(33):6667-78.
Bo Wu, Fengsong Wang, Jiahai Zhang, Zhiyong Zhang, Liying Qin, Junhui Peng, Fudong Li, Jianping Liu, Guowei Lu, Qingguo Gong, Xuebiao Yao, Jihui Wu#, Yunyu Shi#.Identification and structural basis for a novel interaction between Vav2 and Arap3.J Struct Biol.2012 Oct;180(1):84-95.
Lei Liu, Su Qin,Jiahai Zhang,Peng Ji,Yunyu Shi#,Jihui Wu#. (2012)Solution structure of an atypical PHD finger in BRPF2 and its interaction with DNA.J Struct Biol.2012 Oct;180(1):165-73.
Jie Wen, Pei Zhou#, Jihui Wu#.Efficient acquisition of high-resolution 4-D diagonal-suppressed methyl–methyl NOESY for large proteins.J Magn Reson.2012 May;218:128-32.
Xinxin Li, Kaixian Liu, Fudong Li, Juncheng Wang, Hongda Huang, Jihui Wu#,Yunyu Shi#.Structure of the C-terminal tandem BRCT repeats of Rtt107 reveals a critical role in the interaction with γH2A during DNA damage repair.J Biol Chem.2012 Mar 16;287(12):9137-46.
Yu Qiu, Wen Zhang, Chen Zhao, Yan Wang, Weiwei Wang, Jiahai Zhang, Zhiyong Zhang, Guohong Li, Yunyu Shi, Xiaoming Tu#, Jihui Wu#.Solution structure of the Pdp1 PWWP domain reveals its unique binding sites for methylated H4K20 and DNA.Biochem J.2012 Mar 15;442(3):527-38.
Zhenhua Shao, Fudong Li, Shirley M.-H. Sy, Wei Yan, Zhiyong Zhang, Deshun Gong, Bin Wen, Michael S.Y. Huen, Qingguo Gong, Jihui Wu#, Yunyu Shi#.Specific recognition of phosphorylated tail of H2AX by the tandem BRCT domains of MCPH1 revealed by complex structure.J Struct Biol.2012 Feb;177(2):459-68.
2011
Weiwei Wang, Lijun Wang, Yang Zou, Jiahai Zhang, Qingguo Gong, Jihui Wu#, Yunyu Shi# (2011)Cooperation of Escherichia coli Hfq hexamers in DsrA binding.Genes & Development. 25(19):2106-2117.
Hu H, Liu Y, Wang M, Fang J, Huang H, Yang N, Li Y, Wang J, Yao X, Shi Y, Li G, Xu R# (2011)Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.Genes Dev. 25(9):901-906.
Su Qin, Lei Jin, Jiahai Zhang, Lei Liu, Peng Ji, Mian Wu, Jihui Wu and Yunyu Shi. (2011)Recognition of unmodified histone H3 by the first PHD finger of Bromodomain-PHD finger protein 2 provides insights into the regulation of histone acetyltransferases MOZ and MORF.Journal of Biological Chemistry. 286(42):36944-36955
Jianping Liu, Jiahai Zhang, Qingguo Gong, Peng Xiong, Hongda Huang, Bo Wu, Guowei Lu, Jihui Wu and Yunyu Shi. (2011)Solution structure of the tandem SH2 domains from Spt6 and their binding to the phosphorylated RNA polymerase II C-terminal domain.Journal of Biological Chemistry, 286(33):29218-29226
Jie Wen, Jihui Wu, and Pei Zhou. (2011)Sparsely sampled high-resolution 4-D experiments for efficient backbone resonance assignment of disordered proteins.Journal of Magnetic Resonance, 209(1): 94-100
Fangming Wu, Yiwei Liu, Zhiqiang Zhu, Hongda Huang, Bo Ding, Jihui Wu, and Yunyu Shi (2011)The 1.9 ? crystal structure of Prp20p from Saccharomyces cerevisiae and its binding properties to Gsp1p and histones.Journal of Structural Biology, 174(1):213-222
Peng Ji, Guang Yang, Jiahai Zhang, Jiawen Wu, Zhengjun Chen, Qingguo Gong, Jihui Wu, Yunyu Shi. (2011)Solution structure of the second PDZ domain of zonula occludens 1.Proteins: Structure, Function, and Bioinformatics,79(4):1342-1346
2010
Bin Rui, Tie Shen, Hong Zhou, Jianping Liu, Jiusheng Chen, Xiaosong Pan, Haiyan Liu, Jihui Wu, Haoran Zheng and Yunyu Shi. (2010)A systematic investigation of Escherichia coli central carbon metabolism in response to superoxide stress.BMC Systems Biology, 4: 122.
Yiwei Liu*, Hongda Huang*, Bo O. Zhou, Shan-Shan Wang, Yingxia Hu, Xu Li, Jianping Liu, Jianye Zang, Liwen Niu, Jihui Wu, Jin-Qiu Zhou, Maikun Teng#, and Yunyu Shi#. (2010)Structural analysis of Rtt106p reveals a DNA-binding role required for heterochromatin silencing.Journal of Biological Chemistry, 285(6):4251-62.
Xingsheng Wang, Shaojie Zhang, Jiahai Zhang, Xiaojuan Huang, Chao Xu, Weiwei Wang, Zhijun Liu, Jihui Wu, and Yunyu Shi. (2010)A large intrinsically disordered region in SKIP and its disorder-order transition induced by PPIL1 binding revealed by NMR.Journal of Biological Chemistry, 285(7):4951-63.
2009
Xiaojuan Huang, Monique Beullens, Jiahai Zhang, Yi Zhou, Emilia Nicolaescu, Bart Lesage, Qi Hu, Jihui Wu, Mathieu Bollen, and Yunyu Shi. (2009)Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21.Journal of Biological Chemistry, 284(37):25375-87.
Jingjun Hong , Jiahai Zhang , Zhijun Liu , Su Qin , Jihui Wu and Yunyu Shi. (2009)Solution Structure of S. cerevisiae PDCD5like protein and its promoting role in H2O2-induced apoptosis in yeast.Biochemistry, 48(29):6824-34.
Zhijun Liu, Jiahai Zhang, Xingsheng Wang, Yanwei Ding, Jihui Wu and Yunyu Shi. (2009)Temperature-induced partially unfolded state of hUBF HMG Box-5: Conformational and dynamic investigations of the Box-5 thermal intermediate ensemble.Proteins: Structure, Function, and Bioinformatics, 77(2):432-47.
Tie Shen, Weiqun Shen, Ying Xiong, Haiyan Liu, Haoran Zheng, Hong Zhou, Bin Rui, Jianping Liu, Jihui Wu, Yunyu Shi. (2009)Increasing the accuracy of mass isotopomer analysis through calibration curves constructed using biologically synthesized compounds.Journal of Mass Spectrometry, 44(7):1066-80.
2008
Ying Liu, Xiqing Wang, Jiahai Zhang, Hongda Huang, Bo Ding, Jihui Wu, and Yunyu Shi. (2008)Structural Basis and Binding Properties of the Second Bromodomain of Brd4 with Acetylated Histone Tails.Biochemistry, 47 (24): 6403–6417
Jiangxin Liu, Jiahai Zhang, Yinshan Yang, Hongda Huang, Weiqun Shen, Qi Hu, Xingsheng Wang, Jihui Wu, and Yunyu Shi. (2008)Conformational change upon ligand binding and dynamics of the PDZ domain from leukemia-associated Rho guanine nucleotide exchange factor.Protein Science, 17:1003-1014.
Fangming Wu, Jiahai Zhang, Jianping Sun, Hongda Huang, Peng Ji, Wangsheng Chu, Meijuan Yu, Feifei Yang, Ziyu Wu, Jihui Wu and Yunyu Shi. (2008)Solution structure of human DESR1, a CSL zinc-binding protein.Proteins: Structure, Function, and Bioinformatics, 71(1) 514-518.
2007
Xiaogang Niu, Quan Chen, Jiahai Zhang, Weiqun Shen, Yunyu Shi, and Jihui Wu. (2007)Interesting Structural and Dynamical Behaviors Exhibited by the AF-6 PDZ Domain upon Bcr Peptide Binding.Biochemistry, 46 (51):15042 -15053
Qi Hu, Weiqun Shen, Hongda Huang, Jiangxin Liu, Jiahai Zhang, Xiaojuan Huang, Jihui Wu, and Yunyu Shi. (2007)Insight into the Binding Properties of MEKK3 PB1 to MEK5 PB1 from Its Solution Structure.Biochemistry46(47):13478-89.
Hongda Huang , Jiahai Zhang , Weiqun Shen , Xingsheng Wang , Jiawen Wu , Jihui Wu and Yunyu Shi. (2007)Solution structure of the second bromodomain of Brd2 and its specific interaction with acetylated histone tails.BMC Structural Biology, 7:57.
Zhe Shi, Sartaj Tabassum, Wei Jiang, Jiahai Zhang, Suvigya Mathur, Jihui Wu and Yunyu Shi.(2007)Identification of a Potent Inhibitor of Human Dual-Specific Phosphatase, VHR, from Computer-Aided and NMR-Based Screening to Cellular Effects.ChemBioChem, 8(17):2092-2099.
Jiawen Wu, Yinshan Yang, Jiahai Zhang, Peng Ji, Wenjing Du, Peng Jiang, Dinghai Xie, Hongda Huang, Mian Wu, Guangzhao Zhang, Jihui Wu and Yunyu Shi. (2007)Domain-swapped dimerization of the second PDZ domain of ZO2 may provide a structural basis for the polymerization of claudins.Journal of Biological Chemistry, 282(49):35988-35999.
Xianju Jin, Jiahai Zhang, Haiming Dai, Hongbin Sun, Dandan Wang, Jihui Wu and Yunyu Shi.(2007)Investigation of the four cooperative unfolding units existing in the MICAL-1 CH domain.Biophysical Chemistry, 129, 269-278.
Quan Chen, Xiaogang Niu, Yingqi Xu, Jihui Wu, and Yunyu Shi. (2007)Solution structure and backbone dynamics of the AF-6 PDZ domain/Bcr peptide complex.Protein Science, 16, 1053-1062.
Hongbin Sun, Jiangxin Liu, Jiahai Zhang, Weiqun Shen, Hongda Huang, Chao Xu, Haiming Dai, Jihui Wu and Yunyu Shi. (2007)Solution structure of BRD7 bromodomain and its interaction with acetylated peptides from histone H3 and H4.Biochemical and Biophysical Research Communications358, 435-441.
Jiahai Zhang, Xiang Li, Bo Yao, Weiqun Shen, Hongbin Sun, Chao Xu, Jihui Wu and Yunyu Shi. (2007)Solution structure of the first SH3 domain of human vinexin and its interaction with vinculin peptides.Biochemical and Biophysical Research Communications357, 931-937.
Weiqun Shen, Chao Xu, Wei Huang, Jiahai Zhang, Justin E. Carlson, Xiaoming Tu, Jihui Wu, and Yunyu Shi.(2007)Solution Structure of Human Brg1 Bromodomain and Its Specific Binding to Acetylated Histone Tails.Biochemistry46, 2100-2110.
Dandan Wang, Jiahai Zhang, Xianju Jin, Jihui Wu, and Yunyu Shi. (2007)Investigation of the Structural Stability of hUBF HMG Box 5 by Native-State Hydrogen Exchange.Biochemistry46,1293 -1302.
Bo Yao, Jiahai Zhang, Haiming Dai, Jianping Sun, Yuanyuan Jiao, Yajun Tang, Jihui Wu and Yunyu Shi. (2007)Solution structure of the second SH3 domain of human CMS and a newly identified binding site at the C-terminus of c-Cbl.Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 1774, 35-43.
2006
Haiming Dai, Wei Huang, Jian Xu, Bo Yao, Shangmin Xiong, Husheng Ding, Yajun Tang, Haiyan Liu, Jihui Wu and Yunyu Shi. (2006)Binding model of human coactosin-like protein with filament actin revealed by mutagenesis.Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics1764, 1688-700.
Sun, H. B., Dai, H. M., Zhang, J. H., Jin, X. J., Xiong, S. M., Xu, J., Wu, J. H. and Shi, Y. Y. (2006)Solution structure of calponin homology domain of Human MICAL-1.Journal of Biomolecular Nmr36, 295-300.
Li, X., Zhang, J. H., Cao, Z. X., Wu, J. H. and Shi, Y. Y. (2006)Solution structure of GOPC PDZ domain and its interaction with the C-terminal motif of neuroligin.Protein Science15, 2149-2158.
Xu, J. J., Zhang, J. H., Wang, L., Zhou, J., Huang, H. D., Wu, J. H., Zhong, Y., and Shi, Y. Y. (2006)Solution structure of Urm1 and its implications for the origin of protein modifiers.Proceedings of the National Academy of Sciences of the United States of America103, 11625-11630.
Man, N., Cheng, Y. Y., Xu, T. W., Ding, Y., Wang, X. M., Li, Z. W., Chen, Z. C., Huang, G. Y., Shi, Y. Y., and Longping, W. (2006)Dendrimers as potential drug carriers. Part II. Prolonged delivery of ketoprofen by in vitro and in vivo studies.European Journal of Medicinal Chemistry41, 670-674.
Xu, C., Zhang, J. H., Huang, X. J., Sun, J. P., Xu, Y. Q., Tang, Y. J., Wu, J. H., Shi, Y. Y., Huang, Q. H., and Zhang, Q. H. (2006)Solution structure of human peptidyl prolyl isomerase-like protein 1 and insights into its interaction with SKIP.Journal of Biological Chemistry281, 15900-15908.
2005
Zhang, X. C., Zhang, J. H., Li, X., Xu, J. J., Huang, H. D., Chen, Q., Wu, J. H., and Shi, Y. Y. (2005)Compact molten globule-like state of hUBF HMG Box1 at extremely low pH.Biochimica Et Biophysica Acta-Proteins and Proteomics1748, 66-73.
Ding, H. S., Yang, Y. D., Zhang, J. H., Wu, J. H., Liu, H. Y., and Shi, Y. Y. (2005)Structural basis for SUMO-E2 interaction revealed by a complex model using docking approach in combination with NMR data.Proteins-Structure Function and Bioinformatics61, 1050-1058.
Sun, J. P., Zhang, J. H., Wu, F. M., Xu, C., Li, S. J., Zhao, W., Wu, Z. Y., Wu, J. H., Zhou, C. Z., and Shi, Y. Y. (2005)Solution structure of Kti11p from Saccharomyces cerevisiae reveals a novel zinc-binding module.Biochemistry44, 8801-8809.
Zhang, X. C., Xu, Y. Q., Zhang, J. H., Wu, J. H., and Shi, Y. Y. (2005)Structural and dynamic characterization of the acid-unfolded state of hUBF HMG box 1 provides clues for the early events in protein folding.Biochemistry44, 8117-8125.
Xu, C., Zheng, P. Z., Shen, S. H., Xu, Y. Q., Wei, L., Gao, H. J., Wang, S. N., Zhu, C. R., Tang, Y. J., Wu, J. H., Zhang, Q. H., and Shi, Y. Y. (2005)NMR structure and regulated expression in APL cell of human SH3BGRL3.Febs Letters579, 2788-2794.
Zhou, H. Y., Xu, Y. Q., Yang, Y. D., Huang, A. D., Wu, J. H., and Shi, Y. Y. (2005)Solution structure of AF-6 PDZ domain and its interaction with the C-terminal peptides from neurexin and Bcr.Journal of Biological Chemistry280, 13841-13847.
Xu, C., Xu, Y. Q., Tang, Y. J., Wu, J. H., Shi, Y. Y., Huang, Q. H., and Zhang, Q. H. (2005)Letter to the Editor: Backbone and side chain assignments of human peptidylprolyl isomerase like 1 (hPPIL1).Journal of Biomolecular Nmr31, 179-180.
Ding, H. S., Xu, Y. Q., Chen, Q., Dai, H. M., Tang, Y. J., Wu, J. H., and Shi, Y. Y. (2005)Solution structure of human SUMO-3 C47S and its binding surface for Ubc9.Biochemistry44, 2790-2799.
Wang, C. G., Cai, Z., Lu, W. Y., Wu, J. H., Xu, Y. Q., Shi, Y. Y., and Chi, C. W. (2005)A novel short-chain peptide BmKX from the chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination.Toxicon45, 309-319.
Dai, H. M., Wu, J. H., Xu, Y. Q., Tang, Y. J., Ding, H. S., and Shi, Y. Y. (2004)Letter to the Editor: H-1, C-13 and N-15 resonance assignments and the secondary structures of human Coactosin like protein (hCLP) D123N.Journal of Biomolecular Nmr29, 455-456.
Cai, Z., Xu, C. Q., Xu, Y. Q., Lu, W. Y., Chi, C. W., Shi, Y. Y., and Wu, J. H. (2004)Solution structure of BmBKTx1, a new BKCa1 channel blocker from the Chinese scorpion Buthus martensi Karsch.Biochemistry43, 3764-3771.
Xiao, Y. Z., Chen, Q., Hang, J., Shi, Y. Y., Xiao, Y. Z., Wu, J., Hong, Y. Z., and Wang, Y. P. (2004)Selective induction, purification and characterization of a laccase isozyme from the basidiomycete Trametes sp AH28-2.Mycologia96, 26-35.
Zhang, J. H., Du, D. Z., and Wu, J. H. (2003)Identification for the hydrogen and carbon spectra of cilnidipine by nuclear magnetic resonance.Chinese Journal of Analytical Chemistry31, 1044-1047.
Zhu, J., Zhu, Q. Q., Shi, Y. Y., and Liu, H. Y. (2003)How well can we predict native contacts in proteins based on decoy structures and their energies?Proteins-Structure Function and Genetics52, 598-608.
Zeng, W. Y., Wang, Y. H., Zhang, Y. C., Yang, W. L., and Shi, Y. Y. (2003)Functional significance of conserved glycine 127 in a human dual-specificity protein tyrosine phosphatase.Biochemistry-Moscow68, 634-638.
Wang, J. H., Zhang, Z. Y., Liu, H. Y., and Shi, Y. Y. (2003)Quasiequilibrium unfolding thermodynamics of a small protein studied by molecular dynamics simulation with an explicit water model.Physical Review E67.
Xiao, Y. Z., Tu, X. M., Wang, J., Zhang, M., Cheng, Q., Zeng, W. Y., and Shi, Y. Y. (2003)Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp strain AH28-2.Applied Microbiology and Biotechnology60, 700-707.
Wang, Y. H., Zeng, W. Y., and Shi, Y. Y. (2003)Mutation of arginines near the active site Cys(124) of human dual-specificity phosphatase and its effect on the enzymatic activity.Acta Biochimica Et Biophysica Sinica35, 149-153.
Yang, W. L., Xu, Y. Q., Wu, J. H., Zeng, W. Y., and Shi, Y. Y. (2003)Solution structure and DNA binding property of the fifth HMG box domain in comparison with the first HMG box domain in human upstream binding factor.Biochemistry42, 1930-1938.
2001
Xu, Y. Q., Yang, W. L. Wu, J. H. and Shi, Y. Y. (2002)Solution Structure of the First HMG Box Domain in Human Upstream Binding Factor.Biochemistry41, 5415-5420.
Tu, X. M., Wu, J. H., Xu, Y. Q., and Shi, Y. Y. (2001)H-1, C-13 and N-15 resonance assignments and secondary structure of ADR6 DNA-binding domain.Journal of Biomolecular Nmr21, 187-188.
Liu, S. S., Yin, C. S., Shi, Y. Y., Cai, S. X., and Li, Z. L. (2001)MEDV-13 for QSAR studies on the COX-2 inhibition by indomethacin amides and esters.Chinese Journal of Chemistry19, 751-756.
Han, J. G., and Shi, Y. Y. (2001)A computational study on geometries, electronic structures and ionization potentials of MSi15 (M = Cr, Mo, W) clusters by density functional method.Chemical Physics266, 33-40.
Zhang, Z. Y., Zhu, Y. J., and Shi, Y. Y. (2001)Molecular dynamics simulations of urea and thermal-induced denaturation of S-peptide analogue.Biophysical Chemistry89, 145-162.
Zeng, W. Y., Zhang, X. C., Tu, X. M., Guang, S. H., Xiao, Y. Z., and Shi, Y. Y. (2001)Expression and purification of the DNA-binding domain of the human transcription factor E2F1.Protein Expression and Purification21, 99-104.
