張大偉(中科院天津工業生物技術研究所研究員)

張大偉(中科院天津工業生物技術研究所研究員)

張大偉，中科院天津工業生物技術研究所，研究員，博士生導師。主要研究方向是蛋白質合成細胞工廠及工業微生物菌種改造升級。

基本介紹

  • 中文名：張大偉
  • 國籍：中國
  • 民族：漢族
  • 畢業院校：北京化工大學
  • 職業：科研工作者
  • 主要成就：中國產學研合作創新獎 （2018）
  • 職稱：研究員
人物簡介,研究方向,代表論著,承擔科研項目情況,獲獎及榮譽,人物訪談,

人物簡介

2007年獲北京化工大學生物化工專業博士學位
2007年至2008年，美國威斯康星大學密爾沃基分校，博士院棄乘駝後研究
2008年至凳樂2012年，美國加州理工學院，博士承槓簽後研究
2012年，中科院天津工業生物技術研究所，研究員，博士府元煉生導師

研究方向

1. 微生歸諒拒芝物代謝與工業菌種創建：主要利用微生物為細胞工廠，採用分子遺傳，合成生物技術，生物感測技術，組學技術，高通量篩選等方法，合成維生素，棕鞏民胺基酸，及高附加值化合物。
2. 蛋白表達系統與蛋白分泌機制：建立蛋白表達平台，包括大腸桿菌，枯草芽孢桿菌，酵母菌表達系統，實現蛋白質胞內表達或分泌表達，為酶製劑和醫藥蛋白表達提供平台，並開發新型表達系統。基礎研究方面主要研祝棗察究蛋白分泌機制，蛋白分泌新途徑，構建新型分泌系統等。

代表論著

  1. Yali Cui, Huina Dong, Yuanyuan Ma*, Dawei Zhang*.Strategies for Applying Nonhomologous End Joining-Mediated Genome Editing in Prokaryotes. ACS Synthetic Biology. 2019: September 17
  2. Wu J,Liu Y,Zhao S,Sun J,Jin Z*,Zhang D*. Application of the Dynamic Regulation to Increase L-Phenylalanine Production inEscherichia coli. J Microbiol Biotechnol.2019 May 27. doi: 10.4014/jmb.1901.01058
  3. Jiadi Li, Xinli Li Yuanming Gai, Yumei Sun*, Dawei Zhang*. Evolution of E. coli Phytase for Increased Thermostability Guided by Rational Parameters. Journal of Microbiology and Biotechnology. 2019 DOI:10.4014/jmb.1811.11017
  4. Bai D, Ding D, Li J, Cong L*,Zhang D*. Pinpointing the L-phenylalanine binding sites of TyrR using biosensors and computer-aided simulation. Biotechnol Lett. 2019 Jan 24.
  5. Fu G,Liu J,Li J,Zhu BW,Zhang D*. Systematic screening of optimal signal peptides for secretory production of heterologous proteins in Bacillus subtilis. J Agric Food Chem.2018 Nov 22.
  6. Dandan Li, Gang Fu, Ran Tu, Zhaoxia Jin* and Dawei Zhang*. High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components. Microb Cell Fact (2019) 18:17
  7. Huan Fang, Dong Li, Jie Kang, Pingtao Jiang, Jibin Sun, Dawei Zhang*. Metabolic engineering ofEscherichia coli for de novo biosynthesis of vitamin B12. Nature Communications. (2018) volume9, Articlenumber:4917(Breakthrough)
  8. Cai Y , Xia M, Dong H, Qian Y, Zhang T, Zhu B, Wu J, Zhang D*. Engineering a vitamin B12 high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti.
BMC Biotechnol. 2018 May 11;18(1):27. doi: 10.1186/s12896-018-0441-2.
  1. Yuanming Gai, Jingqi Chen, Shibin Zhang, Beiwei Zhu andDawei Zhang*. Property Improvement of α-Amylase from Bacillus stearothermophilus by Deletion of Amino Acid Residues Arginine 179 and Glycine 180. Food Technology & Biotechnology. 2018. January 22, doi: 10.17113/ftb.56.01.18.5448.
  2. Yuanye Chen, Yongfei Liu, Dongqin Ding, Lina Cong*, Dawei Zhang*. Rational design and analysis of an Escherichia coli strain for high，efficiency tryptophan production. Journal of Industrial Microbiology & Biotechnology. 2018. February 4 doi.org/10.1007 /s10295 -018-2020-x.
  3. Liu Y, Xu Y, Ding D, Wen J, Zhu B, Zhang D*. Genetic engineering of Escherichia coli to improve L-phenylalanine production. BMC Biotechnol. 2018 Jan 30;18(1):5. doi: 10.1186/ s12896-018-0418-1.
  4. Yiran Xu, Yongfei Liu, Feiran Li, Guoqiang Cao, Ping Zheng, Jibin Sun, Jianping Wen and Dawei Zhang*. Identification of a new gene yecC involved in threonine export in Escherichia coli. FEMS Microbiology Letters. 2017Sep 15;364(17). doi: 10.1093/femsle/fnx174
  5. Jie Yue, Gang Fu, Dawei Zhang*, Jianping Wen. A new maltose-inducible high-performance heterologous expression system in Bacillus Subtilis. Biotechnol Lett.2017 Aug;39(8): 1237-1244. doi: 10.1007/s10529-017-2357-7.
  6. Song, Yafeng; Fu, Gang; Dong, Huina; Li, Jianjun*; Du, Yuguang ; Zhang, Dawei*. High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization. Journal of Agricultural and Food Chemistry 2017 Mar 29;65(12): 2540-2548, DOI:10.1021/acs.jafc.6b05528.
  7. Liu, Yongfei; Zhuang, Yinyin; Ding, Dongqin; Xu, Yiran; Sun, Jibin; Zhang, Dawei*. Biosensor-based evolution and elucidation of a biosynthetic pathway in Escherichia coli. ACS Synthetic Biology. 2017 May 19;6(5):837-848. doi: 10.1021/acssynbio.6b00328.
  8. Jingqi Chen; Zhaoxia Jin; Yuanming Gai; Jibin Sun; Dawei Zhang*. A food-grade expression system for D-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase- encoding selection marker. Bioresources and Bioprocessing. 2017;4(1):9. DOI: 10.1186/s40643-017-0139-7.
  9. Zhao L, Chen J, Sun J,Zhang D*, Multimer recognition and secretion by the non-classical secretion pathway in Bacillus subtilis. Scientific Reports.2017 Mar 9; 7:44023. doi: 10.1038/srep44023.
  10. Huan Fang; Jie Kang; Dawei Zhang*. Microbial production of vitamin B12: A review and future perspectives. Microbial Cell Factories. 2017 Jan 30;16(1):15. doi: 10.1186/ s12934- 017-0631-y. (Highly accessed)
  11. Ding D,Liu Y,Xu Y,Zheng P,Li H,Zhang D*,Sun J. Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro. Scientific Reports.2016 Aug 25;6:32208. doi: 10.1038/srep32208
  12. Chen J,Zhu Y,Fu G,Song Y,Jin Z,Sun Y*,Zhang D*. High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis. J Ind Microbiol Biotechnol.2016 Nov;43(11):1577-1591
  13. Song Y, Nikoloff JM, Fu G, Chen J, Li Q, Xie N, Zheng P, Sun J,Zhang D*. Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications. PLoS One. 2016 Jul 5;11(7):e0158447. doi: 10.1371/journal. pone.0158447
  14. JingqiChen, LiuqunZhao, GangFu, WenjuanZhou, YuanxiaSun, PingZheng, JibinSun and DaweiZhang*. A novel strategy for protein production using non-classical secretion pathway inBacillus subtilis. Microbial Cell Factories. 2016 Apr 28;15:69. DOI:10.1186/s12934-016 -0469-8.
  15. Huina Dong, Sha Li, Huan Fang, Miaomiao Xia, Ping Zheng*, Dawei Zhang*, Jibin Sun. A Newly Isolated and Identified Vitamin B12 Producing Strain—Sinorhizobium meliloti 320. Bioprocess and Biosystems Engineering. 2016 Oct;39(10):1527-37. DOI：10.1007/s00449-016-1628-3.
  16. HuanFang,HuinaDong, TaoCai,PingZheng,HaixingLi,DaweiZhang*and JibinSun. Invitrooptimizationofenzymesinvolvedinprecorrin-2synthesisusingresponsesurfacemethodology. PloSOne. 2016DOI：10.1371/journal.pone.0151149.
  17. Jingqi Chen,Gang Fu,Yuanming Gai,Ping Zheng,Dawei Zhang* andJianping Wen. Combinatorial Sec pathway analysis for improved heterologous protein secretion inBacillus subtilis: identification of bottlenecks by systematic gene overexpression. Microbial Cell Factories.2015,14:92. (Highly accessed)
  18. Ya’nan Liu, Qinggang Li, Ping Zheng*, Zhidan Zhang, Yongfei Liu, Cunmin Sun, Guoqiang Cao, Wenjuan Zhou, Xiaowei Wang, Dawei Zhang, Tongcun Zhang, Jibin Sun* and Yanhe Ma. Developing a high-throughput screening method forthreonine overproduction based onan artificial promoter Microbial Cell Factories.2015, (2015) 14:121
  19. Yanfei Zhang, Qinglong Meng, Hongwu Ma*, Yongfei Liu, Guoqiang Cao, Xiaoran Zhang, Ping_Zheng, Jibin Sun, Dawei Zhang, Wenxia Jiang, Yanhe Ma. Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis, Microbial Cell Factories. 2015. 14:92 .
  20. Jingqi Chen,Yuanming Gai,Gang Fu,Wenjuan Zhou,Dawei Zhang*, Jianping Wen. Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnol Lett.2015 Apr;37(4):899-906. doi: 10.1007/s10529-014-1755-3
  21. Yafeng Song, Jonas M. Nikoloff, Dawei Zhang*. Improving Protein Production on the Level of Regulation both of Expression and Secretion Pathways in Bacillus subtilis. J. Microbiol. Biotechnol. 2015. 25(7): 963~977.
  22. Huina Dong, Yongfei Liu, Xin Zu, Ning Li, Feiran Li, Dawei Zhang*.An enzymatic assay for high-throughput screening of cytidine-producing microbial strains. PLOS One. 2015 Mar 27;10(3):e0121612. doi: 10.1371/journal.pone.0121612
  23. Huina Dong, Xin Zu, Ping Zheng, Dawei Zhang*. Arapidenzymaticassay for high-throughput screeningofadenosine-producingstrains. Microbial Biotechnology. 2015 Mar;8(2):230-8. doi: 10.1111/1751-7915.12189.
  24. Yongfei Liu, Feiran Li,Xiaoran Zhang,Guoqiang Cao,Wenxia Jiang,Yuxiu Sun,Ping Zheng,Dawei Zhang*. A fast and sensitive coupled enzyme assay for the measurement of L-threonine and application to high-throughput screening of threonine-overproducing strains. Enzyme and Microbial Technology. 2014 Dec;67:1-7. doi: 10.1016/j.enzmictec.2014.08.008.
  25. Haifeng Zhang, Gang Fu, Dawei Zhang*. Cloning, characterization and production of a novel lysozyme by different expression hosts. J. Microbiol. Biotechnol.2014; 24(10): 1405~1412.
  26. Huina Dong, and Dawei Zhang*. Current development in genetic engineering strategies of Bacillus species, Microb Cell Fact, 2014, 13: 63. (Highly accessed)

承擔科研項目情況

1. 國家重點研發計畫：高版本模式微生物底盤細胞
2. 自然基金面上項目：利用SRP抑制子挖掘蛋白轉運新途徑
3. 天津市傑出青年科學基金：通用蛋白表達系統構建與機制研究
4. 中科院科技網路服務STS項目：維生素生物製造
5. 企業重大橫向：維生素菌種改造技術

獲獎及榮譽

1. 國家重點研發計畫：高版本模式微生物底盤細胞
2. 自然基金面上項目：利用SRP抑制子挖掘蛋白轉運新途徑
3. 天津市傑出青年科學基金：通用蛋白表達系統構建與機制研究
4. 中科院科技網路服務STS項目：維生素生物製造
5. 企業重大橫向：維生素菌種改造技術

人物訪談

張大偉：排兵布陣，瞄準微生物“球門”
2018-02-12 來源： 科技日報 孫玉松
張大偉和團隊主要從事酶的蛋白表達和微生物菌種合成研究。在合成生物研究領域，酶的蛋白表達研究是項非常枯燥的工作。在研究過程中，需要不斷收集大量數據。
他的研究團隊有20多人，張大偉常用“足球理論”來管理團隊。進實驗室前，預先分工，布置任務；實驗時，每個環節都要落實到人；試驗後，總結、評估也有專人來落實……在張大偉看來，只有團隊緊密配合才能順利完成實驗。
BMC Biotechnol. 2018 May 11;18(1):27. doi: 10.1186/s12896-018-0441-2.
  1. Yuanming Gai, Jingqi Chen, Shibin Zhang, Beiwei Zhu andDawei Zhang*. Property Improvement of α-Amylase from Bacillus stearothermophilus by Deletion of Amino Acid Residues Arginine 179 and Glycine 180. Food Technology & Biotechnology. 2018. January 22, doi: 10.17113/ftb.56.01.18.5448.
  2. Yuanye Chen, Yongfei Liu, Dongqin Ding, Lina Cong*, Dawei Zhang*. Rational design and analysis of an Escherichia coli strain for high，efficiency tryptophan production. Journal of Industrial Microbiology & Biotechnology. 2018. February 4 doi.org/10.1007 /s10295 -018-2020-x.
  3. Liu Y, Xu Y, Ding D, Wen J, Zhu B, Zhang D*. Genetic engineering of Escherichia coli to improve L-phenylalanine production. BMC Biotechnol. 2018 Jan 30;18(1):5. doi: 10.1186/ s12896-018-0418-1.
  4. Yiran Xu, Yongfei Liu, Feiran Li, Guoqiang Cao, Ping Zheng, Jibin Sun, Jianping Wen and Dawei Zhang*. Identification of a new gene yecC involved in threonine export in Escherichia coli. FEMS Microbiology Letters. 2017Sep 15;364(17). doi: 10.1093/femsle/fnx174
  5. Jie Yue, Gang Fu, Dawei Zhang*, Jianping Wen. A new maltose-inducible high-performance heterologous expression system in Bacillus Subtilis. Biotechnol Lett.2017 Aug;39(8): 1237-1244. doi: 10.1007/s10529-017-2357-7.
  6. Song, Yafeng; Fu, Gang; Dong, Huina; Li, Jianjun*; Du, Yuguang ; Zhang, Dawei*. High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization. Journal of Agricultural and Food Chemistry 2017 Mar 29;65(12): 2540-2548, DOI:10.1021/acs.jafc.6b05528.
  7. Liu, Yongfei; Zhuang, Yinyin; Ding, Dongqin; Xu, Yiran; Sun, Jibin; Zhang, Dawei*. Biosensor-based evolution and elucidation of a biosynthetic pathway in Escherichia coli. ACS Synthetic Biology. 2017 May 19;6(5):837-848. doi: 10.1021/acssynbio.6b00328.
  8. Jingqi Chen; Zhaoxia Jin; Yuanming Gai; Jibin Sun; Dawei Zhang*. A food-grade expression system for D-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase- encoding selection marker. Bioresources and Bioprocessing. 2017;4(1):9. DOI: 10.1186/s40643-017-0139-7.
  9. Zhao L, Chen J, Sun J,Zhang D*, Multimer recognition and secretion by the non-classical secretion pathway in Bacillus subtilis. Scientific Reports.2017 Mar 9; 7:44023. doi: 10.1038/srep44023.
  10. Huan Fang; Jie Kang; Dawei Zhang*. Microbial production of vitamin B12: A review and future perspectives. Microbial Cell Factories. 2017 Jan 30;16(1):15. doi: 10.1186/ s12934- 017-0631-y. (Highly accessed)
  11. Ding D,Liu Y,Xu Y,Zheng P,Li H,Zhang D*,Sun J. Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro. Scientific Reports.2016 Aug 25;6:32208. doi: 10.1038/srep32208
  12. Chen J,Zhu Y,Fu G,Song Y,Jin Z,Sun Y*,Zhang D*. High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis. J Ind Microbiol Biotechnol.2016 Nov;43(11):1577-1591
  13. Song Y, Nikoloff JM, Fu G, Chen J, Li Q, Xie N, Zheng P, Sun J,Zhang D*. Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications. PLoS One. 2016 Jul 5;11(7):e0158447. doi: 10.1371/journal. pone.0158447
  14. JingqiChen, LiuqunZhao, GangFu, WenjuanZhou, YuanxiaSun, PingZheng, JibinSun and DaweiZhang*. A novel strategy for protein production using non-classical secretion pathway inBacillus subtilis. Microbial Cell Factories. 2016 Apr 28;15:69. DOI:10.1186/s12934-016 -0469-8.
  15. Huina Dong, Sha Li, Huan Fang, Miaomiao Xia, Ping Zheng*, Dawei Zhang*, Jibin Sun. A Newly Isolated and Identified Vitamin B12 Producing Strain—Sinorhizobium meliloti 320. Bioprocess and Biosystems Engineering. 2016 Oct;39(10):1527-37. DOI：10.1007/s00449-016-1628-3.
  16. HuanFang,HuinaDong, TaoCai,PingZheng,HaixingLi,DaweiZhang*and JibinSun. Invitrooptimizationofenzymesinvolvedinprecorrin-2synthesisusingresponsesurfacemethodology. PloSOne. 2016DOI：10.1371/journal.pone.0151149.
  17. Jingqi Chen,Gang Fu,Yuanming Gai,Ping Zheng,Dawei Zhang* andJianping Wen. Combinatorial Sec pathway analysis for improved heterologous protein secretion inBacillus subtilis: identification of bottlenecks by systematic gene overexpression. Microbial Cell Factories.2015,14:92. (Highly accessed)
  18. Ya’nan Liu, Qinggang Li, Ping Zheng*, Zhidan Zhang, Yongfei Liu, Cunmin Sun, Guoqiang Cao, Wenjuan Zhou, Xiaowei Wang, Dawei Zhang, Tongcun Zhang, Jibin Sun* and Yanhe Ma. Developing a high-throughput screening method forthreonine overproduction based onan artificial promoter Microbial Cell Factories.2015, (2015) 14:121
  19. Yanfei Zhang, Qinglong Meng, Hongwu Ma*, Yongfei Liu, Guoqiang Cao, Xiaoran Zhang, Ping_Zheng, Jibin Sun, Dawei Zhang, Wenxia Jiang, Yanhe Ma. Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis, Microbial Cell Factories. 2015. 14:92 .
  20. Jingqi Chen,Yuanming Gai,Gang Fu,Wenjuan Zhou,Dawei Zhang*, Jianping Wen. Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnol Lett.2015 Apr;37(4):899-906. doi: 10.1007/s10529-014-1755-3
  21. Yafeng Song, Jonas M. Nikoloff, Dawei Zhang*. Improving Protein Production on the Level of Regulation both of Expression and Secretion Pathways in Bacillus subtilis. J. Microbiol. Biotechnol. 2015. 25(7): 963~977.
  22. Huina Dong, Yongfei Liu, Xin Zu, Ning Li, Feiran Li, Dawei Zhang*.An enzymatic assay for high-throughput screening of cytidine-producing microbial strains. PLOS One. 2015 Mar 27;10(3):e0121612. doi: 10.1371/journal.pone.0121612
  23. Huina Dong, Xin Zu, Ping Zheng, Dawei Zhang*. Arapidenzymaticassay for high-throughput screeningofadenosine-producingstrains. Microbial Biotechnology. 2015 Mar;8(2):230-8. doi: 10.1111/1751-7915.12189.
  24. Yongfei Liu, Feiran Li,Xiaoran Zhang,Guoqiang Cao,Wenxia Jiang,Yuxiu Sun,Ping Zheng,Dawei Zhang*. A fast and sensitive coupled enzyme assay for the measurement of L-threonine and application to high-throughput screening of threonine-overproducing strains. Enzyme and Microbial Technology. 2014 Dec;67:1-7. doi: 10.1016/j.enzmictec.2014.08.008.
  25. Haifeng Zhang, Gang Fu, Dawei Zhang*. Cloning, characterization and production of a novel lysozyme by different expression hosts. J. Microbiol. Biotechnol.2014; 24(10): 1405~1412.
  26. Huina Dong, and Dawei Zhang*. Current development in genetic engineering strategies of Bacillus species, Microb Cell Fact, 2014, 13: 63. (Highly accessed)

承擔科研項目情況

1. 國家重點研發計畫：高版本模式微生物底盤細胞
2. 自然基金面上項目：利用SRP抑制子挖掘蛋白轉運新途徑
3. 天津市傑出青年科學基金：通用蛋白表達系統構建與機制研究
4. 中科院科技網路服務STS項目：維生素生物製造
5. 企業重大橫向：維生素菌種改造技術

獲獎及榮譽

1. 國家重點研發計畫：高版本模式微生物底盤細胞
2. 自然基金面上項目：利用SRP抑制子挖掘蛋白轉運新途徑
3. 天津市傑出青年科學基金：通用蛋白表達系統構建與機制研究
4. 中科院科技網路服務STS項目：維生素生物製造
5. 企業重大橫向：維生素菌種改造技術

人物訪談

張大偉：排兵布陣，瞄準微生物“球門”
2018-02-12 來源： 科技日報 孫玉松
張大偉和團隊主要從事酶的蛋白表達和微生物菌種合成研究。在合成生物研究領域，酶的蛋白表達研究是項非常枯燥的工作。在研究過程中，需要不斷收集大量數據。
他的研究團隊有20多人，張大偉常用“足球理論”來管理團隊。進實驗室前，預先分工，布置任務；實驗時，每個環節都要落實到人；試驗後，總結、評估也有專人來落實……在張大偉看來，只有團隊緊密配合才能順利完成實驗。

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