囊泡轉運活體動態分析及其定向轉運分子機制的研究

科研成果

序號	標題	類型	作者
1	Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation in Picea meyeri Pollen Tube Growth	期刊論文	Baluska, Frantisek\|Chen, Tong\|Lin, Jinxing\|Chen, Yanmei\|Li, Xiaojuan\|Huang, Mei\|Samaj, Jozef\|Zheng, Maozhong\|Wu, Xiaoqin\|
2	Probing and tracking organelles in living plant cells	期刊論文	Wang XH\|Zheng MZ\|von Wangenheim D\|Ji WQ\|Lin JX\|Samaj J\|Chen T\|
3	Calmodulin Binds to Extracellular Sites on the Plasma Membrane of Plant Cells and Elicits a Rise in Intracellular Calcium Concentration	期刊論文	Liu, Peng\|Sun, Daye\|Lin, Jinxing\|Lucas, William J.\|Fang, Xiaohong\|Zheng, Maozhong\|Chen, Bo\|Cui, Sujuan\|Wang, Yuqing\|Liu, Chun-Ming\|Wang, Qinli\|
4	Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis	期刊論文	Jin, Jing Bo\|Huang, Shanjin\|Song, Xiufen\|Zhu, Yingfang\|Li, Ruili\|Wang, Qinli\|Lin, Jinxing\|Wang, Yuqing\|Liu, Chun-Ming\|
5	Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation	期刊論文	Wang, X.\|Li, X\|He, Q.\|Lin, J.\|Luu, D. T.\|Li, R.\|Maurel, C.\|Yang, Y.\|Fang, X.\|
6	Actin Turnover Is Required for Myosin-Dependent Mitochondrial Movements in Arabidopsis Root Hairs	期刊論文	Logan, David C.\|Chen, Tong\|Baluska, Frantisek\|Mueller, Jens\|Wang, Qinli\|Samaj, Jozef\|Wang, Feng\|Beck, Martina\|Zheng, Maozhong\|Wang, Xiaohua\|Wang, Yuqing\|Lin, Jinxing\|
7	Disruption of actin filaments induces mitochondrial Ca2+ release to the cytoplasm and [Ca2+](c) changes in Arabidopsis root hairs	期刊論文	Wang, Yuqing\|Zhu, Yingfang\|Ling, Yu\|Baluska, Frantisek\|Zhang, Haiyan\|Samaj, Jozef\|Liu, Peng\|Wang, Qinli\|Lin, Jinxing\|
8	隱失波螢光顯微鏡及其在植物細胞生物學中的套用	期刊論文	王欽麗\|王曉華\|XUE Yi-qun\|FAN Lu-sheng\|林金星\|WANG Qin-li\|薛軼群\|LIN Jin-xing\|WANG Xiao-hua\|范路生\|
9	Nitric oxide modulates the influx of extracellular Ca2+ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes	期刊論文	Lin, Jinxing\|Zheng, Maozhong\|Samaj, Jozef\|Chen, Tong\|Liu, Peng\|Zhang, Chunyang\|Hao, Huaiqing\|Baluska, Frantisek\|Wang, Yuhua\|
10	Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana	期刊論文	Wan, Yinglang\|Ill, William M. Ash\|Fan, Lusheng\|Lin, Jinxing\|Kim, Myung K.\|Hao, Huaiqin\|
11	量子點標記及其在植物細胞生物學中的套用	期刊論文	李曉娟\|王欽麗\|蘇月\|林金星\|
12	Lipid microdomain polarization is required for NADPH oxidase-dependent ROS signaling in Picea meyeri pollen tube tip growth	期刊論文	Lin, Jin-Xing\|Li, Rui-Li\|Zhang, Liang\|Niehaus, Karsten\|Wang, Qin-Li\|Liu, Peng\|Baluska, Frantisek\|Samaj, Jozef\|
13	Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are Required for Cell Plate Formation	期刊論文	Zhang, Haiyan\|Hao, Huaiqing\|Lin, Jinxing\|Jin, Jing Bo\|Liu, Peng\|Zhang, Liang\|

項目摘要

囊泡轉運過程在細胞的增殖和發育過程中具有十分重要的生物學意義,該方面的研究是國家自然科學基金委生命科學部十一五學科發展戰略和優先資助領域。本項目將在我們過去工作的基礎上，擬進一步利用植物單細胞體系- - 花粉管為實驗系統，針對過去研究存在的主要問題和不足, 著重開展三方面的研究：(1)囊泡轉運的活體動態分析；（2）細胞骨架極性動態組裝和馬達蛋白對囊泡定向轉運的影響；（3）囊泡定向轉運的分子機制研究。在上述研究中擬採用多種新方法和新技術，包括隱失波螢光顯微術、轉盤式掃描共聚焦顯微術、基因體外定點突變技術、RNAi 技術、光激活螢光蛋白標記技術等。藉助這些新技術和新方法，不僅可以更加深入地對花粉管囊泡轉運進行活體動態觀測及相關參數分析，揭示細胞骨架極性動態組裝和馬達蛋白在囊泡定向轉運過程中的作用，而且有助於闡明囊泡是如何實現定向轉運，為進一步探討囊泡轉運的生物學機制提供依據。

囊泡轉運活體動態分析及其定向轉運分子機制的研究

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科研成果

項目摘要

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