《梨自交不親和基因產物S-RNase介導花粉管程式性死亡的機制》是張紹鈴為項目負責人，南京農業大學為依託單位的面上項目。
基本介紹
- 中文名：梨自交不親和基因產物S-RNase介導花粉管程式性死亡的機制
- 項目類別：面上項目
- 項目負責人 ：張紹鈴
- 依託單位 ：南京農業大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Distribution and metabolism of ascorbic acid in pear fruits (Pyrus pyrifolia Nakai cv. Aikansui) | 期刊論文 | Huang WJ|Qing GH|Zhang HP|Wu J|Zhang SL| |
2 | 薔薇科果樹自交不親和性分子機制研究進展 | 期刊論文 | 張紹鈴|吳巨友|吳俊|齊永傑|高永彬|ZHANG Shao-ling,WU Ju-you,WU Jun,QI Yong-jie,GAO Y| |
3 | Molecular Determinants and Mechanisms of Gametophytic Self-Incompatibility in Fruit Trees of Rosaceae | 期刊論文 | Wu, Jun|Gu, Chao|Khan, M. Awais|Wu, Juyou|Gao, Yongbing|Wang, Chunlei|Korban, Schuyler S.|Zhang, Shaoling| |
4 | A method for isolating mitochondria from pear pollen tubes | 期刊論文 | Wang CL|Gao YB|Zhou HS|Zhang SL| |
5 | Identification of differentially expressed genes in a spontaneous mutant of 'Nanguoli' pear (Pyrus ussuriensis Maxim) with large fruit | 期刊論文 | Zhang SJ|Wu J|Chen H|Gu C|Tao ST|Wu JY|Zhang SL| |
6 | Potassium flux in the pollen tubes was essential in plant sexual reproduction | 期刊論文 | Wu JY|Jin C|Zhang SL| |
7 | Characterization of the S-RNase genomic DNA allele sequence in Prunus speciosa and P. pseudocerasus | 期刊論文 | Gu C|Wu J|Zhang SJ|Yang YN|Wu HQ|Tao ST|Zhang SL| |
8 | Low temperature inhibits pollen viability by alteration of actin cytoskeleton and regulation of pollen plasma membrane ion channels in Pyrus pyrifolia | 期刊論文 | Wu, Ju-You|Jin, Cong|Qu, Hai-Yong|Tao, Shu-Tian|Xu, Guo-Hua|Wu, Jun|Wu, Hua-Qing|Zhang, Shao-Ling| |
9 | 細胞鬆弛素B和鬼筆環肽對梨花粉管鈣離子濃度和尖端鈣離子通道的影響 | 期刊論文 | 劉珠琴|吳巨友|姜雪婷|徐國華|張紹鈴| |
10 | Reciprocal regulation of Ca2+-activated outward K+ channels of Pyrus pyrifolia pollen by heme and carbon monoxide | 期刊論文 | Wu, Ju-You|Qu, Hai-Yong|Xu, Guo-Hua|Wu, Jun|Shao-Ling Zhang| |
11 | Low temperature inhibits pollen tube growth by disruption of both tip-localized reactive oxygen species and endocytosis in Pyrus bretschneideri Rehd | 期刊論文 | Gao Yong-Bin|Wang Chun-Lei|Wu Ju-You|Zhou Hong-Sheng|Jiang Xue-Ting|Wu Jun|Zhang Shao-Ling| |
12 | Evaluation of candidate reference genes for real time quantitative PCR normalization in pear fruit | 期刊論文 | Wu T|Zhang RP|Gu C|Wu JY|Wan HJ|Zhang SJ|Zhang SL| |
13 | Fast loading ester fluorescent Ca2+ and pH indicators into pollen of Pyrus pyrifolia | 期刊論文 | Qu, Haiyong|Jiang, Xueting|Shi, Zebin|Liu, Lianmei|Zhang, Shaoling| |
14 | The activity of plasma membrane hyperpolarization-activated Ca2+ channels during pollen development of Pyrus pyrifolia | 期刊論文 | Wu, Juyou|Jin, Cong|Qu, Haiyong|Jiang, Xueting|Wu, Jun|Xu, Guohua|Zhang, Shaoling| |
15 | Apoplastic calmodulin promotes self-incompatibility pollen tube growth by enhancing calcium influx and reactive oxygen species concentration in pyrus pyrifolia | 期刊論文 | Jiang X, Gao Y, Zhou H, Chen J, Wu J, Zhang S| |
16 | Inheritance of hetero-diploid pollen S-haplotype in self-compatible tetraploid Chinese cherry (Prunus pseudocerasus Lindl) | 期刊論文 | Gu C|Liu QZ|Yang YN|Zhang SJ|Khan MA|Wu J|Zhang SL| |
17 | Molecular analysis of eight SFB alleles and a new SFB-like gene in Prunus pseudocerasus and Prunus speciosa | 期刊論文 | Gu C|Wu J|Zhang SJ|Yang YN|Wu HQ|Khan MA|Zhang SL|Liu QZ| |
18 | cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia | 期刊論文 | Wu, Juyou|Qu, Haiyong|Jin, Cong|Shang, Zhongling|Wu, Jun|Xu, Guohua|Gao, Yongbing|Zhang, Shaoling| |
19 | A method to isolate male gametic nuclei from pear pollen tubes | 期刊論文 | Gao, Y. B.|Wang, C. L.|Wu, J. Y.|Wu, J.|Zhou, H. S.|Zhang, S. L.| |
項目摘要
在對梨自交不親和性生理及分子機理研究的基礎上，綜合套用雷射細胞生物學及生物化學等的研究手段和技術，從花粉管骨架、超微結構及生理生化代謝等方面深入研究梨花柱S-RNase介導花粉管程式性死亡的機制。內容包括花柱S-RNase介導自花花粉管程式性死亡過程的花粉管骨架（微絲及微管）、細胞核、液泡、線粒體、高爾基體等超微結構變化特點，花粉管核DNA的凝聚與片段化特性，花柱S-RNase與花粉管凋亡誘導因子（AIF）、類Caspase、細胞色素C、VPE等的協同作用，花柱S-RNase介導的花粉管囊泡轉運動態（胞吞/胞吐）及分布特性等。將闡明花粉管感受花柱S-RNase的'毒害'而凋亡的'應答'反應特點，揭示配子體型自交不親和性反應中花柱S-RNase介導自花花粉管程式性死亡的細胞超微結構與生化代謝機制，完善薔薇科植物自交不親和性機理的理論體系，有重要學術理論和潛在套用價值。
