酶切位點

酶切位點（Restriction Enzyme cutting site）：DNA上一段鹼基的特定序列，限制性內切酶能夠識別出這個序列並在此將DNA序列切成兩段。

可能存在同尾酶，不同酶的識別序列不同，有的可能能識別多個酶切位點比如STY1識別序列有WW。

基本介紹

• 中文名：酶切位點
• 外文名：Restriction Enzyme cutting site
• 所屬類型：DNA上一段鹼基的特定序列
• 功能：將DNA序列切成兩段

保護鹼基表

不同內切酶對識別位點以外最少保護鹼基數目的要求（在本表中沒有列出的酶，則通常需在識別位點兩端至少加上6個保護鹼基，以確保酶切反應的進行。）

酶	寡核苷酸序列	鏈長	切割率%
			2 hr	20 hr
Acc I	GGTCGACC; CGGTCGACCG; CCGGTCGACCGG	8; 10; 12	0; 0; 0	0; 0; 0
Afl III	CACATGTG; CCACATGTGG; CCCACATGTGGG	8; 10; 12	0; >90; >90	0; >90; >90
Asc I	GGCGCGCC; AGGCGCGCCT; TTGGCGCGCCAA	8; 10; 12	>90; >90; >90	>90; >90; >90
Ava I	CCCCGGGG; CCCCCGGGGG; TCCCCCGGGGGA	8; 10; 12	50; >90; >90	>90; >90; >90
BamH I	CGGATCCG; CGGGATCCCG; CGCGGATCCGCG	8; 10; 12	10; >90; >90	25; >90; >90
Bgl II	CAGATCTG; GAAGATCTTC; GGAAGATCTTCC	8; 10; 12	0; 75; 25	0; >90; >90
BssH II	GGCGCGCC; AGGCGCGCCT; TTGGCGCGCCAA	8; 10; 12	0; 0; 50	0; 0; >90
BstE II	GGGT(A/T)ACCC	9	0	10
BstX I	AACTGCAGAACCAATGCATTGG; AAAACTGCAGCCAATGCATTGGAA; CTGCAGAACCAATGCATTGGATGCAT	22; 24; 27	0; 25; 25	0; 50; >90
Cla I	CATCGATG; GATCGATC; CCATCGATGG; CCCATCGATGGG	8; 8; 10; 12	0; 0; >90; 50	0; 0; >90; 50
EcoR I	GGAATTCC; CGGAATTCCG; CCGGAATTCCGG	8; 10; 12	>90; >90; >90	>90; >90; >90
Hae III	GGGGCCCC; AGCGGCCGCT; TTGCGGCCGCAA	8; 10; 12	>90; >90; >90	>90; >90; >90
Hind III	CAAGCTTG; CCAAGCTTGG; CCCAAGCTTGGG	8; 10; 12	0; 0; 10	0; 0; 75
Kpn I	GGGTACCC; GGGGTACCCC; CGGGGTACCCCG	8; 10; 12	0; >90; >90	0; >90; >90
Mlu I	GACGCGTC; CGACGCGTCG	8; 10	0; 25	0; 50
Nco I	CCCATGGG; CATGCCATGGCATG	8; 14	0; 50	0; 75
Nde I	CCATATGG; CCCATATGGG; CGCCATATGGCG; GGGTTTCATATGAAACCC; GGAATTCCATATGGAATTCC; GGGAATTCCATATGGAATTCCC	8; 10; 12; 18; 20; 22	0; 0; 0; 0; 75; 75	0; 0; 0; 0; >90; >90
Nhe I	GGCTAGCC; CGGCTAGCCG; CTAGCTAGCTAG	8; 10; 12	0; 10; 10	0; 25; 50
Not I	TTGCGGCCGCAA; ATTTGCGGCCGCTTTA; AAATATGCGGCCGCTATAAA; ATAAGAATGCGGCCGCTAAACTAT; AAGGAAAAAAGCGGCCGCAAAAGGAAAA	12; 16; 20; 24; 28	0; 10; 10; 25; 25	0; 10; 10; 90; >90
Nsi I	TGCATGCATGCA; CCAATGCATTGGTTCTGCAGTT	12; 22	10; >90	>90; >90
Pac I	TTAATTAA; GTTAATTAAC; CCTTAATTAAGG	8; 10; 12	0; 0; 0	0; 25; >90
Pme I	GTTTAAAC; GGTTTAAACC; GGGTTTAAACCC; AGCTTTGTTTAAACGGCGCGCCGG	8; 10; 12; 24	0; 0; 0; 75	0; 25; 50; >90
Pst I	GCTGCAGC; TGCACTGCAGTGCA; AACTGCAGAACCAATGCATTGG; AAAACTGCAGCCAATGCATTGGAA; CTGCAGAACCAATGCATTGGATGCAT	8; 14; 22; 24; 26	0; 10; >90; >90; 0	0; 10; >90; >90; 0
Pvu I	CCGATCGG; ATCGATCGAT; TCGCGATCGCGA	8; 10; 12	0; 10; 0	0; 25; 10
Sac I	CGAGCTCG	8	10	10
Sac II	GCCGCGGC; TCCCCGCGGGGA	8; 12	0; 50	0; >90
Sal I	GTCGACGTCAAAAGGCCATAGCGGCCGC; GCGTCGACGTCTTGGCCATAGCGGCCGCGG; ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA	28; 30; 32	0; 10; 10	0; 50; 75
Sca I	GAGTACTC; AAAAGTACTTTT	8; 12	10; 75	25; 75
Sma I	CCCGGG; CCCCGGGG; CCCCCGGGGG; TCCCCCGGGGGA	6; 8; 10; 12	0; 0; 10; >90	10; 10; 50; >90
Spe I	GACTAGTC; GGACTAGTCC; CGGACTAGTCCG; CTAGACTAGTCTAG	8; 10; 12; 14	10; 10; 0; 0	>90; >90; 50; 50
Sph I	GGCATGCC; CATGCATGCATG; ACATGCATGCATGT	8; 12; 14	0; 0; 10	0; 25; 50
Stu I	AAGGCCTT; GAAGGCCTTC; AAAAGGCCTTTT	8; 10; 12	>90; >90; >90	>90; >90; >90
Xba I	CTCTAGAG; GCTCTAGAGC; TGCTCTAGAGCA; CTAGTCTAGACTAG	8; 10; 12; 14	0; >90; 75; 75	0; >90; >90; >90
Xho I	CCTCGAGG; CCCTCGAGGG; CCGCTCGAGCGG	8; 10; 12	0; 10; 10	0; 25; 75
Xma I	CCCCGGGG; CCCCCGGGGG; CCCCCCGGGGGG; TCCCCCCGGGGGGA	8; 10; 12; 14	0; 25; 50; >90	0; 75; >90; >90