翁小成，博士，武漢大學化學與分子科學學院教授，博士生導師，主要研究方向為核酸化學生物學。
基本介紹
- 中文名：翁小成
- 國籍：中國
- 出生日期：1981年5月
- 畢業院校：武漢大學
- 學位/學歷：博士
- 職業：教師
- 專業方向：化學生物學
人物經歷,教學情況,研究領域,項目課題,學術成果,發表論文,
人物經歷
1998.09-2002.07，武漢大學化學與分子科學學院，化學基地班，學士
2002.07-2007.11，武漢大學化學與分子科學學院，有機化學，博士，導師：周翔教授
2007.11-2010.11，武漢大學化學與分子科學學院，講師
2010.11-2018.09，武漢大學化學與分子科學學院，副教授
2013.11-2016.11 美國芝加哥大學 訪問學者，博士後，導師：何川 教授
2018.09- 至今，武漢大學化學與分子科學學院，教授
教學情況
《生物化學》本科生 主講
《化學生物學導論》本科生 參與
《化學生物學進展》研究生 主講
《化學生物學基礎》研究生 參與
研究領域
長期從事核酸化學生物學的研究。基於化學探針等化學技術，融合分子生物學、基因組學、細胞生物學等手段，開發核酸表觀遺傳修飾、核酸結構以及核酸/蛋白質相互作用的研究技術，並開展相應的功能研究，以及開拓在臨床疾病診斷方面的套用。
項目課題
1. 主持國家自然科學基金青年科學基金項目1項：《化學小分子作為DNA序列和結構特異性識別劑的研究》，20802055
2. 主持高等學校博士學科點專項科研新教師基金1項：《具有序列選擇性的可誘導DNA交聯劑的研究》
3. 主持湖北省自然科學基金1項：《生物相容性的糖苷類卟啉的合成及其抗腫瘤活性研究》
學術成果
1. 2020年湖北省科學技術獎勵自然科學一等獎 (核酸的分子識別和調控，第2完成人)
2. 2018年獲批國家自然科學基金優秀青年基金資助
3. 2010年湖北省科學技術獎勵自然科學獎一等獎（以核酸為靶目標的小分子的設計、合成及其生物活性研究，第2完成人）
4. 獲武漢大學第五屆青年教師教學競賽一等獎 (2010年)學術成果
發表論文
1. Ruitu Lyu, Tong Wu, Allen C. Zhu, Diana C. West-Szymanski, Xiaocheng Weng, Mengjie Chen, Chuan He*. KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal-assisted labeling. Nature Protocols, 2021, accepted.
2. Yafen Wang, Shaoqing Han, Ruoxi Ran, Anling Li, Huanyu Liu, Mingjun Liu, Yongwei Duan, Xiong Zhang, Zhigang Zhao, Shihui Song, Xiaocheng Weng*, Songmei Liu*, Xiang Zhou*, A longitudinal sampling study of transcriptomic and epigenetic profiles in patients with thrombocytopenia syndrome. Nature Communications 2021, 12, 5629.
3. Wang, Y. F.; Zhang, X.; Han, S. Q.; Yang, W.; Chen, Z. G.; Wu, F.; Liu, J. Z.; Weng, X. C.*; Zhou, X.*, Base-Resolution Analysis of Deoxyuridine at the Genome Scale Based on the Artificial Incorporation Modified Nucleobase. Acs Central Science 2021, 7 (6), 973-979. (封面文章)
4. Wang, Q.; Tan, K. Y.; Wang, H.; Shang, J. H.; Wan, Y. Q.; Liu, X. Q.; Weng, X. C.; Wang, F. A.*, Orthogonal Demethylase-Activated Deoxyribozyme for Intracellular Imaging and Gene Regulation. Journal of the American Chemical Society 2021, 143 (18), 6895-6904.
5. Wang, B. Y.; Zhang, X.; Wang, Y. F.; Chen, K.; Wang, F.; Weng, X. C.*; Zhou, X.*, One-pot fluorescent assay for sensitive detection of APOBEC3A activity. RSC Chemical Biology 2021, 2 (4), 1201-1205.
6. Wang, B. Y.; Chen, Y.; Zhang, X.; Jiang, Z. R.; Wang, Y. F.; Chen, K.; Wang, F.; Weng, X. C.*; Zhou, X.*, A far-red emissive two-photon fluorescent probe for quantification of uracil in genomic DNA. Chemical Communications 2021, 57 (22), 2784-2787.
7. Li, W.; Yang, H. M.; Wang, Y. F.; Weng, X. C.*; Wang, F.*, Highly sensitive detection of 6mA at single-base resolution based on A-C mismatch. Analyst 2021, 146 (14), 4450-4453.
8. Huang, J. G.; Zhao, R. Q.; Mo, J.; Wang, F.; Weng, X. C.*; Zhou, X. *, N-3-Kethoxal-Based Bioorthogonal Intracellular RNA Labeling. Chembiochem 2021, 22 (9), 1559-1562.
9. Zhang, Y. J.; Zhang, Q. Y.; Weng, X. C.; Du, Y. H.*; Zhou, X., NEase-based amplification for detection of miRNA, multiple miRNAs and circRNA. Analytica Chimica Acta 2021, 1145, 52-58.
10.Huang, J. G.; Zhao, R. Q.; Qin, S. S.; Yang, S. X.;Li, W.; Mo, J.; Wang, F.; Du, Y. H.; Weng, X. C.*; Zhou, X., 4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA. Chembiochem 2021, 22, 212-216.
11.Mo, J.; Chen, Z. G.; Qin, S. S.; Li, S.; Liu, C. G.; Zhang, L.; Ran, R. X.; Kong, Y.; Wang, F.; Liu, S. M.; Zhou, Y.; Zhang, X. L.; Weng, X. C.*; Zhou, X.*, TRADES: Targeted RNA Demethylation by SunTag System. Advanced Science 2020, 7 (19), 202001402.
12.Chen, Yuqi; Wu, Fan; Chen, Zonggui; He, Zhiyong; Wei, Qi; Zeng, Weiwu; Chen, Kun; Xiao, Feng; Yuan, Yushu; Weng, Xiaocheng; Zhou, Yu; Zhou, Xiang*, Acrylonitrile-Mediated Nascent RNA Sequencing for Transcriptome-Wide Profiling of Cellular RNA Dynamics. Advanced Science 2020, 7 (8), 1900997.
13.Weng, X.; Gong, J.; Chen, Y.; Wu, T.; Wang, F.; Yang, S.; Yuan, Y.; Luo, G.; Chen, K.; Hu, L.; Ma, H.; Wang, P.; Zhang, Q. C.*; Zhou, X.*; He, C.*, Keth-seq for transcriptome-wide RNA structure mapping. Nature Chemical Biology 2020, 16 (5), 489-492.
14.Yang, S.; Wu, F.; Peng, S.; Wang, F.; Chen, Y.; Yuan, Y.; Weng, X.*; Zhou, X., A m6A sensing method by its impact on the stability of RNA double helix. Chemistry & Biodiversity 2020, https://doi.org/10.1002/cbdv.202000050.
15.Liu, Y. Q.; Zhang, X.; Liu, M.; Xu, F.; Zhang, Q. Y.; Zhang, Y. J.; Weng, X. C.; Liu, S. M.; Du, Y. H.*; Zhou, X.*, Direct detection of circRNA in real samples using reverse transcription-rolling circle amplification. Analytica Chimica Acta 2020, 1101, 169-175.
16.Zou, G. R.; Liu, C. X.; Fang, Z. T.; Jia, S. K.; Yang, W.; Zhang, X.; Chen, M. K.; Tian, T.; Weng, X. C.; Zhou, X.*, Photocaged probes for spatiotemporal imaging. Sensors and Actuators B-Chemical 2019, 288, 113-119.
17.Li, W.; Zeng, W. W.; Chen, Y.; Wang, F.; Wu, F.; Weng, X. C.*; Zhou, X.*, Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity. Analyst 2019, 144 (15), 4472-4476.
18.Li, W.; Wang, F.; Chen, Y.; Weng, X. C.*; Zhou, X., A sensitive and radiolabeling-free method for pseudouridine detection. Analytical Biochemistry 2019, 581.
19.Chen, Y.; Yang, S.; Peng, S.; Li, W.; Wu, F.; Yao, Q.; Wang, F.; Weng, X.*; Zhou, X.*, N1-methyladenosine detection with CRISPR-Cas13a/C2c2. Chemical Science 2019, 10, 2975-2979
20.Zhong, X.; Yu, J. Y.; Frazier, K.; Weng, X. C.; Li, Y.; Cham, C. M.; Dolan, K.; Zhu, X. R.; Hubert, N.; Tao, Y.; Lin, F. F.; Martinez-Guryn, K.; Huang, Y.; Wang, T.; Liu, J. Z.; He, C.*; Chang, E. B.*; Leone, V.*, Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m(6)A mRNA Methylation. Cell Reports 2018, 25 (7), 1816-+.
21.Yuan, Y. S.; Hong, T. T.; Chen, Y.; Wang, Y. F.; Qiu, X. P.; Zheng, F.; Weng, X. C.*; Zhou, X.*, Luminescence Sensing for Qualitative and Quantitative Detection of 5-Methylcytosine. Analytical Chemistry 2018, 90 (16), 10064-10068.
22.Wu, F.; Liu, W. T.; Yang, S. X.; Yao, Q.; Chen, Y.; Weng, X. C.*; Zhou, X.*, An aptamer-based ligation-triggered rolling circle amplification strategy for ATP detection and imaging in situ. Journal of Photochemistry and Photobiology a-Chemistry 2018, 355, 114-119.
23.Wang, Y. F.; Liu, C. X.; Zhang, X.; Yang, W.; Wu, F.; Zou, G. R.; Weng, X. C.*; Zhou, X.*, Gene specific-loci quantitative and single-base resolution analysis of 5-formylcytosine by compound-mediated polymerase chain reaction. Chemical Science 2018, 9 (15), 3723-3728.
24.Wang, Y. F.; Liu, C. X.; Wu, F.; Zhang, X.; Liu, S.; Chen, Z. G.; Zeng, W. W.; Yang, W.; Zhang, X. L.; Zhou, Y.; Weng, X. C.; Wu, Z. G.; Zhou, X.*, Highly Selective 5-Formyluracil Labeling and Genome-wide Mapping Using (2-Benzimidazolyl)Acetonitrile Probe. Iscience 2018, 9, 423-+.
25.Su, R.; Dong, L.; Li, C.; Nachtergaele, S.; Wunderlich, M.; Qing, Y.; Deng, X.; Wang, Y.; Weng, X.; Hu, C.; Yu, M.; Skibbe, J.; Dai, Q.; Zou, D.; Wu, T.; Yu, K.; Weng, H.; Huang, H.; Ferchen, K.; Qin, X.; Zhang, B.; Qi, J.; Sasaki, A. T.; Plas, D. R.; Bradner, J. E.; Wei, M.; Marcucci, G.; Jiang, X.; Mulloy, J. C.; Jin, J.*; He, C.*; Chen, J.*, R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m(6)A/MYC/CEBPA Signaling. Cell 2018, 172 (1-2), 90-+.
26.Luo, G. Z.; Hao, Z. Y.; Luo, L. Z.; Shen, M. R.; Sparvoli, D.; Zheng, Y. Q.; Zhang, Z. J.; Weng, X. C.; Chen, K.; Cui, Q.; Turkewitz, A. P.; He, C.*, N-6-methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA. Genome Biology 2018, 19.
27.Hong, T. T.; Yuan, Y. S.; Chen, Z. G.; Xi, K.; Wang, T. L.; Xie, Y. L.; He, Z. Y.; Su, H. M.; Zhou, Y.; Tan, Z. J.; Weng, X. C.*; Zhou, X.*, Precise Antibody -Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution. Journal of the American Chemical Society 2018, 140 (18), 5886-5889.
28.Liu, C.; Wang, Y.; Yang, W.; Wu, F.; Zeng, W.; Chen, Z.; Huang, J.; Zou, G.; Zhang, X.; Wang, S.; Weng, X.; Wu, Z.; Zhou, Y.; Zhou, X.*, Fluorogenic labeling and single-base resolution analysis of 5-formylcytosine in DNA. Chemical Science 2017, 8 (11), 7443-7447.
29.Li, Z.; Weng, H.; Su, R.; Weng, X.; Zuo, Z.; Li, C.; Huang, H.; Nachtergaele, S.; Dong, L.; Hu, C.; Qin, X.; Tang, L.; Wang, Y.; Hong, G.-M.; Huang, H.; Wang, X.; Chen, P.; Gurbuxani, S.; Arnovitz, S.; Li, Y.; Li, S.; Strong, J.; Neilly, M. B.; Larson, R. A.; Jiang, X.; Zhang, P.; Jin, J.*; He, C.*; Chen, J.*, FTO Plays an Oncogenic Role in Acute Myeloid Leukemia as a N-6-Methyladenosine RNA Demethylase. Cancer Cell 2017, 31 (1), 127-141.
30.Xu, X.; Mao, W.; Lin, F.; Hu, J.; He, Z.; Weng, X.; Wang, C.-J.; Zhou, X.*, Enantioselective Diels-Alder reactions using a G-triplex DNA-based catalyst. Catalysis Communications 2016, 74, 16-18.
31.Luo, G.-Z.; Wang, F.; Weng, X.; Chen, K.; Hao, Z.; Yu, M.; Deng, X.; Liu, J.; He, C.*, Characterization of eukaryotic DNA N-6-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. Nature Communications 2016, 7.
32.Wang, X.; Zhao, B. S.; Roundtree, I. A.; Lu, Z.; Han, D.; Ma, H.; Weng, X.; Chen, K.; Shi, H.; He, C.*, N-6-methyladenosine Modulates Messenger RNA Translation Efficiency. Cell 2015, 161 (6), 1388-1399.
