基本介紹
- 中文名：齊以濤
- 畢業院校：中國農業大學
- 學位/學歷：博士
- 職業：教師
- 專業方向：蛋白質SUMO修飾與疾病
- 任職院校：陝西師範大學
- 性別：男
- 職稱：教授
個人經歷,學習經歷,工作經歷,研究方向,主講課程,學術成果,科研成果,學術兼職,研究領域,科研項目,論文,榮譽獎項,
個人經歷
學習經歷
1998-2002 山東農業大學學士
2002-2007 中國農業大學博士
工作經歷
2007-2012 美國德州心臟研究所博士後
2012 -2014 美國德州心臟研究所副研究員
2015-至今陝西師範大學生命科學學院教授
研究方向
蛋白質SUMO修飾與疾病
主講課程
本科生：細胞生物學、細胞生物學實驗、蛋白質工程
研究生：高級細胞生物學、高級生物化學與分子生物學、分子細胞生物學、細胞及分子生物學前沿進展、細胞蛋白質研究方法
學術成果
科研成果
首次發現SUMO特異性蛋白酶SENP2基因缺失導致胚胎心臟發育缺陷，證實了SENP2在胚胎心臟發育中的調控作用，以及SUMO修飾調控PcG複合物的活性，進而調控機體發育和細胞分化的分子機制；首次發現SENP2在myostatin的信號通路及骨骼肌發育中的重要作用；首次發現了SENP2缺失引起鉀離子通道蛋白Kv7的過度SUMO修飾，從而降低Kv7的通道活性，引起顛癇和猝死，證實了SENP2對神經系統及其引起的心臟綜合症有重要影響。相關研究成果發表在《Neuron》（IF: 15.982），Molecular Cell （IF: 14.464）和《Journal of Biological Chemistry》（IF: 4.6）等國際一流刊物，並獲得MD Anderson腫瘤中心內科部基礎科學類“傑出論文獎”。研究成果得到了許多國際主流媒體的關注和報導，如ScienceDaily和EurekAlert等。近年來在Neuron，Molecular Cell，Journal of Biological Chemistry，Journal of Cellular Physiology，Toxicology等國內外學術刊物上發表論文20餘篇。同時，相關成果得到國家和陝西省自然科學基金面上項目的資助。
學術兼職
編委：Biotechnology letters, Bosnian journal of basic medical sciences, Journal of cellular biology and molecular sciences
審稿人：Apoptosis, Bioresources, Biotechnology letters, BMC molecular biology, Bosnian journal of basic medical sciences, Breast cancer research and treatment, Cell and bioscience, Central European journal of biology, Cytotechnology, Evidence-based complementary and alternative medicine, Food and chemical toxicology, Geomicrobiology journal, Molecular and cellular biochemistry, Natural product research, Neuropsychiatric disease and treatment, Plos one, Preparative biochemistry and biotechnology
研究領域
類泛素修飾（SUMOylation）是一種新的蛋白質修飾形式，是影響其所修飾的蛋白質功能與定位的一個關鍵性調節機制。它參與調節多種細胞生命活動過程，包括細胞周期、細胞分裂、DNA損傷修復、細胞內轉運、調節蛋白穩定和基因轉錄等。SUMO修飾是一個動態可逆的過程，由SUMO特異的活化酶（E1）、結合酶（E2）和連線酶（E3）相繼作用來催化完成，其去SUMO修飾的過程（De-SUMOylation）則是由SUMO特異性蛋白酶家族（SENP）來完成。本實驗室主要研究蛋白質的SUMO修飾,特別是由SENP2介導的去SUMO修飾過程和對細胞信號轉導的調控機制，以及它們在發育與疾病過程中的作用與意義。主要研究領域有:
1. SENP2的靶蛋白分子與生物學功能
SENP2的功能是通過對靶蛋白質的去SUMO化，來改變靶蛋白的活性來實現的。因此，研究SENP2的生物學功能，首先要鑑定SENP2的靶蛋白分子。我們通過功能性篩選和基因敲除等策略來尋找和鑑定SENP2的靶蛋白質，並研究SENP2調控靶蛋白的作用與機制，從而來明確SENP2的生物學功能。
2.調控SENP2的細胞信號網路
SENP2的去SUMO化參與了許多細胞生命活性過程，也會受到細胞信號網路的調控。因此我們在鑑定SENP2靶蛋白的基礎上，研究細胞信號通路對這一去SUMO化過程的調節作用與機制，並依此建立SENP2的細胞信號調控網路。
3. SENP2在發育與疾病中的作用與意義
SUMO修飾的靶蛋白分子與發育和疾病有密切的關係，因此SENP2通過這些靶蛋白分子的去SUMO化修飾，必將參與對發育和疾病的調控。我們利用細胞、模式動物及疾病標本，綜合運用分子、細胞和發育生物學以及病理學的知識與手段，研究蛋白質的SUMO修飾在發育與疾病過程中的作用機制，為醫學實踐提供理論基礎。
科研項目
1.主持國家自然科學基金面上項目“SUMO特異性蛋白酶3調控小鼠胚胎心臟發育的機制研究”（81870241，2019.1-2022.12）
2.主持國家自然科學基金面上項目“SUMO修飾調控Kv7.2/Kv7.3鉀離子通道蛋白活性引起癲癇猝死的機制研究”（81671294，2017.1-2020.12）
3.主持陝西省自然科學基金面上項目“SUMO修飾影響迷走神經活性引起癲癇猝死的機制研究”（2017JM3013，2017.1-2018.12）
4.主持中央高校基本科研業務費專項資金項目，“SENP2調控M電流的機制研究”，（GK201603061，2016.1-2017.12）
5.參與中央高校基本科研業務費專項資金項目，“脂醯輔酶A脫氫酶(ACADs)調控三陰性乳腺癌轉移的機制研究”（GK201603064，2016.1-2017.12）
6.參與中央高校基本科研業務費創新團隊項目“細胞凋亡及其清除的分子機制研究”（GK201701005，2017.1-2019.12）
7.陝西師範大學引進人才科研啟動基本資金項目（2015.1-2018.1）
1.Xu Chen, Shanshan Zhang, Jifang Huang, Wanying Dong, Huanjie Shao, Hui Xiao, Jinke Cheng, Hongmei Wu*,Yitao Qi* (*Corresponding author). Hyper-SUMOylation of Kchannels in sudden unexplained death in epilepsy: Isolation and primary culture of dissociated hippocampal neurons from newborn mice for subcellular localization.Methods in molecular biology. 1684 (2017): 63-71.
2.Hongmei Wu, Xu Chen, Jinke Cheng,Yitao Qi*(*Corresponding author). SUMOylation and Potassium Channels: Links to Epilepsy and Sudden Death.Advances in Protein Chemistry and Structural Biology(IF: 3.036). 103 (2016): 295-321.
3.Yitao Qi, Jingxiong Wang, Valerie C. Bomben, Depei Li, Shaorui Chen, Hao Sun, Yutao Xi, John G. Reed, Jinke Cheng, Huilin Pan, Jeffrey L. Noebels, Edward T.H. Yeh. Hyper-SUMOylation of the Kv7 potassium channel abolishes the M current leading to seizures and sudden death.Neuron(IF: 15.054). 83(5) (2014): 1159-1171.
4.Xunlei Kang,Yitao Qi*, Yong Zuo, Qi Wang, Yanqiong Zou, Robert J. Schwartz, Jinke Cheng, Edward T.H. Yeh. (*equal contribution). SUMO-Specific Protease 2 is essential for suppression of polycomb group proteins mediated gene silencing during embryonic development.Molecular Cell(IF: 14.714). 38 (2010): 191-201.
5.Yitao Qi, Yong Zuo, Edward T.H. Yeh, Jinke Cheng. SUMO-specific protease 2 is essential for myostatin expression and myogenesis.Journal of Biological Chemistry(IF: 4.573). 289(6) (2014): 3288-3293.
6.Yitao Qi, Yiding Tu, Di Yang, Qian Chen, Jun Xiao, Yiqiang Chen, Juanling Fu, Xilong Xiao, Zongcan Zhou. Cyclin A but not Cyclin D1 is Essential for c-myc-Modulated Cell Cycle Progression.Journal of Cellular Physiology(IF: 4.155). 210 (2007): 63-71.
7.Zhiqin Wang,Yitao Qi*, Qian Chen, Di Yang, Shusheng Tang, Xi Jin, Jie Gao, Juanling Fu, Zongcan Zhou, Jie Wang, Xilong Xiao. (*equal contribution). Cyclin A is essential for the p53-modulated inhibition from benzo(a)pyrene toxicity in A549 cells.Toxicology(IF: 3.817). 256 (2009): 1-6.
8.Rong Cai, Jianmin Gu, Haipeng Sun, Xiaobing Liu, Wenhan Mei,Yitao Qi, Song Xue, Shuxun Ren, Joseph E. Rabinowitz, Yibin Wang, Edward T.H. Yeh, Jinke Cheng. Induction of SENP1 in myocardium contributes to abnormaities of mitochondria and cardiomyopathy.Journal of Molecular and Cellular Cardiology(IF: 5.68). 79 (2015): 115-122.
9.Xiaohui Yan, Pengfei Li, Yihong Zhan, Miao Qi, Jin Liu, Zhifeng An, Weiwei Yang, Hui Xiao, Hongmei Wu,Yitao Qi, Huanjie Shao. Dihydroartemisinin suppresses STAT3 signaling and Mcl-1 and Survivin expression to potentiate ABT-263-induced apoptosis in Non-small Cell Lung Cancer cells harboring EGFR or RAS mutation.Biochemical Pharmacology(IF: 4.581). 150(2018): 72-85.
10.Jinhui Zhou,Yitao Qi, Qingyun Diao, Liming Wu, Xia Du, Yi Li, Liping Sun. Cytotoxicity of melittin and apamin in human hepatic L02 cells and HepG2 cells in vitro through apoptosis.Toxin reviews(IF: 0.857). 32(4) (2013): 60-67.
11.Di Yang,Yitao Qi, Qian Chen, Zhiqin Wang, Xi Jin, Jie Gao, Juanling Fu, Xilong Xiao, Zongcan Zhou. The over-expression of p53 H179Y residue mutation causes the increase of cyclin A and Cdk4 expression in HELF cells.Molecular and Cellular Biochemistry(IF: 2.669). 304 (2007): 219-226.
12.Qian Chen, Yiqiang Chen,Yitao Qi, Lihua Hao, Shusheng Tang, Xilong Xiao. Chracterization of carbadox-induced mutagenesis using a shuttle vector pSP189 in mammalian cells.Mutation Research-Fundamental and molecular mechanisms of mutagenesis(IF: 3.198). 638 (2008): 11-16.
13.Xingyuan Cao, Mei Dong, Jianzhong Shen, Beibei Wu, Congming Wu, Xiangdang Du, Zhuo Wang,Yitao Qi, Bingyu Li. Tilmicosin and tylosin have anti-inflammatory properties via modulation of COX-2 and iNOS gene expression and production of cytokines in LPS-induced macrophages and monocytes.International Journal of Antimicrobial Agents(IF: 4.307). 27 (2006): 431-438.
14.Yi Li,Yitao Qi, Joan Ritho, Yongxin Zhang, Xiaowei Zheng, Jinhui Zhou, Liping Sun. Characterization of flavonoid glycosides from rapeseed bee pollen using a combination of chromatography, spectrometry and nuclear magnetic resonance with a step-wise separation strategy.Natural Product Research(IF: 1.828). 2016: 30(2): 228-231.
15.Jinhui Zhou,Yitao Qi, Joan Ritho, Yongxin Zhang, Xiaowei Zheng, Liming Wu, Yi Li, Liping Sun. Flavonoid glycosides as floral origin markers to discriminate of unifloral bee pollen by LC-MS/MS.Food Control(IF: 3.496). 57 (2015): 54-61.
16.Jinhui Zhou,Yitao Qi, Joan Ritho, Lili Duan, Liming Wu, Qingyun Diao, Yi Li, Jing Zhao. Analysis of maltooligosaccharides in honey samples by ultra-performance liquid chromatography coupled with evaporative light scattering detection.Food Research International(IF: 3.182). 56 (2014): 260-265.
17.Jinhui Zhou,Yitao Qi, Hongmei Wu, Qingyun Diao, Feifei Tian, Yi Li. Simultaneous determination of trace migration of phthalate esters in honey and royal jelly by gas chromatography with mass spectrometry.Journal of Separation Science(IF: 2.741). 37 (2014): 650-657.
18.Jinhui Zhou,Yitao Qi, Yali Hou, Jing Zhao, Yi Li, Xiaofeng Xue, Liming Wu, Jinzhen Zhang, Fang Chen. Quantitative determination of juvenile hormone III and 20-hydroxyecdysone in queen larvae and drone pupae of apis mellifera by ultrasonic-assisted extraction and liquid chromatography with electrospray ionization tandem mass spectrometry.Journal of Chromatography B-Analytical Technologies in Biomedical and Life Sciences(IF: 2.888). 879 (2011): 2533-2541.
19.Yiqiang Chen, Yanhong Shang, Xiaoping Wu,Yitao Qi, Xilong Xiao. Enzyme-linked immunosorbent assay for the detection of neomycin in milk: effect of hapten heterology on assay sensitivity.Food and Agricultural Immunology(IF: 1.548). 18(2) (2007): 117-128.
20.Jinhui Zhou, Jing Zhao, Suxia Zhang, Jianzhong Shen,Yitao Qi, Xiaofeng Xue, Yi Li, Liming Wu, Jinzhen Zhang, Fang Chen, Lanzhen Chen. Quantification of melittin and apamin in bee venom lyophilized powder from Apis mellifera by liquid chromatography-diode array detector-tandem mass spectrometry.Analytical Biochemistry(IF: 3.236). 404 (2010): 171-178.
