賴德華(中山大學生命科學學院副教授)

賴德華，男，博士，中山大學生命科學學院副教授、碩士生導師。

基本介紹

• 中文名：賴德華
• 畢業院校：中山大學
• 學位/學歷：博士
• 專業方向：分子寄生蟲學及進化
• 任職院校：中山大學

研究方向

分子寄生蟲學及進化

寄生蟲是一類特殊的動物，需要寄生在一個或多個宿主體內。我們不僅研究寄生蟲的形態、生活史、感染力、抗藥性等基本寄生蟲學特徵，還研究基因及蛋白功能與進化。

個人經歷

2013 – 至今	副教授 碩士生導師	中山大學
2014 – 至今	廣東省動物學會理事	廣東省動物學會
2017 – 至今	原生動物學分會理事	中國動物學會
2018 – 至今	廣東省寄生蟲學會理事	廣東省寄生蟲學會
2010 – 2012	居里夫人訪問學者	英國牛津大學
2011 – 2012	英國STEM大使	Researchers in Residence
2008 – 2010	博士後	捷克南波希米亞大學
2003 – 2008	博士	中山大學
1999 – 2003	學士	中山大學

講授課程

濱海動物學實習（本科大一暑假）

生物化學（本科大二）

學術規範與論文寫作（研究生第一年第二學期）

學術成就

一直從事人獸共患寄生蟲的研究，並取得顯著成績，2005年迄今，在PNAS、the Lancet Infectious Diseases和Molecular Biology and Evolution等國際頂級學術期刊雜誌發表論文多篇，SCI論文四十餘篇，影響因子10及以上有10篇，得到寄生生物領域國內外同行認可。

研究成果加深了學術界對寄生生物的生物學特性在細胞，蛋白及分子水平上的認識，在物種形成及進化等巨觀方面做出了積極貢獻，也為寄生蟲的控制及藥物靶點設計提供了重要的理論基礎。

承擔課題

主持的科研基金:

2018–2021國家自然科學基金面上項目(31772445)

利用iNOS基因敲除大鼠研究宿主NO對弓形蟲增殖與分化的調控

2016–2020廣東省自然科學基金傑出青年項目(2016A030306048)

人畜共患病原體錐蟲的遺傳特性及分化調控

2015–2018廣州市珠江科技新星專項項目(201506010011)

人畜共患錐蟲的遺傳特性及分化調控

2015–2018廣東省自然科學基金面上項目(2014A030313164)

利用布氏錐蟲BBCP蛋白研究真核生物中心體的蛋白組成及蛋白功能

2015 – 2017國家自然科學基金青年項目(31402029)

利用布氏錐蟲BBCP蛋白研究真核生物中心體的蛋白組成及蛋白功能

參加的科研基金:

2017–2020國家重點研發計畫畜禽重大疫病防控與高效安全養殖綜合技術研發(2017YFD0500400)畜禽重要胞內寄生原蟲的寄生與免疫機制研究1856萬

2016–2018國家重點研發計畫生物安全關鍵技術專項(2016YFC1200500)重要熱帶病傳播相關的入侵媒介生物及其病原體的生物學特性研究1277萬

2015–2018廣東省自然科學基金重點項目(2015A030311042)

布氏錐蟲分化機理的分子基礎

論文專著

Gao JM#, He ZH#, Xie YT, Hide G, Lai DH*, Lun ZR* (2019) The association between Toxoplasma gondii infection and postpartum blues. J. Affect. Disorders 250:404–409.

Zhang X, Li SJ, Li Z, He CY, Hide G, Lai DH*, Lun ZR* (2019) Cell cycle and cleavage events during in vitro cultivation of bloodstream forms of Trypanosoma lewisi, a zoonotic pathogen. Cell Cycle. 18(5):552–567. doi: 10.1080/15384101.2019.1577651

Bando H, Sakaguchi N, Lee Y, Pradipta A, Ma JS, Tanaka S, Lai DH, Liu J, Lun Z-R, Nishikawa Y, Sasai M and Yamamoto M (2018) Toxoplasma effector TgIST targets host IDO1 to antagonize the IFN-g-induced anti-parasitic response in human cells. Front. Immunol. 9:207 doi: 10.3389/fimmu.2018.02073

An R, Tang Y, Chen L, Cai H, Lai DH, Liu K, Wan L, Gong L, Yu L, Luo Q, Shen J, Lun ZR, Ayala FJ, Du J. (2018) Encephalitis is mediated by ROP18 of Toxoplasma gondii, a severe pathogen in AIDS patients. Pro. Nat. Acad. Sci. USA. 115(23):E5344–E5352. doi: 10.1073/pnas.1801118115

Zhang X, Hong XK, Li SJ, Lai DH, Hide G, Lun ZR, Wen YZ. (2018) The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo. Exp. Parasitol. 184:115–120.

Shen J#, Lai DH#, Wilson RA, Chen YF, Wang LF, Yu ZL, Li MY, He P, Hide G, Sun X, Yang TB, Wu ZD*, Ayala FJ*, Lun ZR*. (2017) Nitric oxide blocks the development of the human parasite Schistosoma japonicum. Proc. Natl. Acad. Sci. USA. 114 (38): 10214–10219. doi: 10.1073/pnas.1708578114. (影響因子: 9.6) 2017.9.5

Hong XK, Zhang X, Fusco OA, Lan YG, Lun ZR, Lai DH*(通訊作者). (2017) PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA. Acta Tropica. 171:207–212. (影響因子: 2.4)

Li K, Zhou S, Guo Q, Chen X, Lai DH, Lun ZR, Guo X. (2017) The eIF3 complex of Trypanosoma brucei: composition conservation does not imply the conservation of structural assembly and subunits function. RNA. 23(3):333–345. doi: 10.1261/rna.058651.116. Mar (影響因子: 3.7)

Li Z, Guo Q, Zheng L, Ji Y, Xie YT, Lai DH, Lun ZR, Suo X, Gao N. (2017) Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii. Cell Res. doi: 10.1038/cr.2017.104. (影響因子: 7.3)

Xie YT, Gao JM, Wu YP, Tang P, Hide G, Lai DH*(通訊作者), Lun ZR*. (2017) Recombinant α-actinin subunit antigens of Trichomonas vaginalis as potential vaccine candidates in protecting against trichomoniasis. Parasits Vectors. 10(1):83. doi: 10.1186/s13071-017-2009-8. 2017 Feb 16 (影響因子: 3.23)

Gao JM, Xie YT, Xu ZS, Chen H, Hide G, Yang TB, Shen JL, Lai DH*(通訊作者), Lun ZR*.(2017) Genetic analyses of Chinese isolates of Toxoplasma gondii reveal a new genotype with high virulence to murine hosts. Vet. Parasitol. 241:52–60. doi: 10.1016/j.vetpar.2017.05.007. 2017 Jul 15. (影響因子: 2.5)

Qin A(共一), Lai DH(共一), Liu Q, Huang W, Wu Y, Chen X, Yan S, Xia H, Hide G, Lun ZR*, Ayala FJ*, Xiang* AP. (2017) Guanylate-binding protein 1 (GBP1) contributes to the immunity of human mesenchymal stromal cells against Toxoplasma gondii. Proc. Natl. Acad. Sci. USA. 114(6):1365–1370. (影響因子: 9.6) 2017.2.7

Wen YZ, Su BX, Lyu SS, Hide G, Lun ZR, Lai DH*. (2016) Trehalose, an easy, safe and efficient cryoprotectant for the parasiticprotozoan Trypanosoma brucei. Acta Tropica 164, 297–302. (影響因子: 2.4) Dec

賴德華，吳娜，謝禕婷，洪曉昆，陳允甫，廖力夫，倫照榮*. (2016)一例輸入性皮膚利什曼病病原體的鑑定. 中國寄生蟲學與寄生蟲病雜誌34(3):295–296,封3. [Lai DH, Wu N, Xie YT, Hong XK, Chen YF, Liao LF, Lun ZR*. (2016) Pathogen Identification in an Imported Case of Cutaneous Leishmaniasis. Chin. J. Parasitol. Parasit. Dis. 34(3):295-296.] Jun 國家核心期刊

Wen YZ, Lun ZR, Zhu XQ, Hide G, Lai DH*. (2016) Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei. Infect. Genet. Evol. 41:56–62. (影響因子: 3.0) July

Lai DH*, Hong XK, Su BX, Liang C, Hide G, Zhang X, Yu X, Lun ZR. (2016) Current status of Clonorchis sinensis and clonorchiasis in China. Trans. R. Soc. Trop. Med. Hyg. 110:21–27. (影響因子: 1.8) Jan

Lin RH, Lai DH*, Zheng LL, Wu J, Lukeš J, Hide G, Lun ZR* (2015). Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen. Parasit. & Vectors 8:665. (影響因子: 3.2) Dec

Lun ZR*, Lai DH, Wen YZ, Zheng LL, Shen JL, Yang TB, Zhou WL, Qu LH, Hide G*, Ayala FJ* (2015) Cancer in the parasitic protozoans Trypanosoma brucei and Toxoplasma gondii. Proc. Natl. Acad. Sci. USA. July 112(29):8835–8842. (影響因子: 9.6)

Flegontov P, Michalek J, Janouskovec J, Lai DH, Jirku M, Hajduskova E, Tomcala A, Otto TD, Keeling PJ, Pain A, Obornik M, Lukes J (2015) Divergent Mitochondrial Respiratory Chains in Phototrophic Relatives of Apicomplexan Parasites. Mol. Biol. Evol. May. 32(5): 1115–1131. DOI: 10.1093/molbev/msv021 (影響因子: 10.4)

Carnes J, Anupama A, Balmer O, Jackson A, Lewis M, Brown R, Cestari1 I, Desquesnes M, Gendrin C, Hertz-Fowler C, Imamura H, Ivens A, Koreny L, Lai DH, MacLeod A, McDermott SM, Merritt C, Monnerat S, Moon W, Myler P, Phan I, Ramasamy G, Sivam D, Lun ZR*, Lukeš J*, Stuart K*, Schnaufer A* (2015) Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty. PLOS Negl. Trop. Dis. 9(1):e3404. (影響因子:4.4)

Gao JM, Yi SQ, Wu MS, Geng GQ, Shen JL, Lu FL, Hide G, Lai DH(共同通訊作者), Lun ZR* (2015) Investigation of infectivity of neonates and adults from different rat strains to Toxoplasma gondii Prugniaud shows both variation which correlates with iNOS and Arginase-1 activity and increased susceptibility of neonates to infection. Exp. Parasitol. 149(1): 47–53. (影響因子: 1.6)

Xie YT, Lai DH, Liu GY, Zhou JL, Lun ZR* (2015) Severe fever with thrombocytopenia syndrome in China. Lancet Infect. Dis. February 15(2): 145. (影響因子: 4)

Lun ZR, Wen YZ, Uzureau P, Lecordier L, Lai DH, Lan YG, Desquesnes M, Geng GQ, Yang TB, Zhou WL, Jannin JG, Simarro PP, Truc P, Vincendeau P, Pays E. (2015) Resistance to normal human serum reveals Trypanosoma lewisi as an underestimated human pathogen. Mol. Biochem. Parasitol. 199(1):58-61. (影響因子: 2.9)

Lai DH, Poropat E, Pravia C, Landoni M, Couto AS, Pérez Rojo FG, Fuchs AG, Dubin M, Elingold I, Rodríguez JB, Ferella M, Esteva MI, Bontempi EJ*, Lukeš J* (2014) Solanesyl diphosphate synthase, an enzyme of the ubiquinone synthetic pathway, is required throughout the life cycle of Trypanosoma brucei. Euk. Cell Feb 13(2): 320–328. (影響因子: 3.6)

Desquesnes M, Holzmuller P, Lai DH, Dargantes A, Lun ZR, Jittapalapong S (2013) Trypanosoma evansi and surra: A review and perspectives on origin, history & distribution, taxonomy, morphology, hosts and pathogenic effects. BioMed. Res. Int. 194176 Aug 19 (影響因子: 2.9)

Desquesnes M, Holzmuller P, Lai DH, Dargantes A, Lun ZR, Jittapalapong S (2013) Trypanosoma evansi and surra: A review and perspectives on transmission, epidemiology and control, impact, and zoonotic aspects. BioMed. Res. Int. 321237 Sep 18 (影響因子: 2.9)

Janouskovec J, Sobotka R(equal contribution), Lai DH (共同第一作者), Flegontov P, Koník P, Komenda J, Ali S, Prásil O, Pain A, Oborník M, Lukes J, Keeling PJ (2013) Split photosystem protein, linear-mapping topology and growth of structural complexity in the plastid genome of Chromera velia. Mol. Biol. Evol. Nov;30(11):2447–62. doi: 10.1093/molbev/mst144. (影響因子: 10.4)

Chen Y, Wen T, Lai DH, Wen YZ, Wu ZD, Yang TB, Yu XB, Hide G, Lun ZR (2013) Development and evaluation of loop-mediated isothermal amplification (LAMP) for rapid detection of Clonorchis sinensis from its first intermediate hosts, freshwater snails. Parasitology. 2013 Sep;140(11):1377–83. doi: 10.1017/S0031182013000498. (影響因子: 2.4)

Lai DH (共同通訊作者), Bontempi EJ(共同通訊作者), Lukeš J (2012) Trypanosoma brucei solanesyl-diphosphate synthase localizes to the mitochondrion. Mol. Biochem. Parasitol. 183(2):189–192. (影響因子: 2.9)

Wang MH, Wen YZ, Wei Y, Lai DH, Bengaly Z, Desquesnes M, Lun ZR (2012) Mitogen-activated protein kinase 5, a novel molecular marker for the identification and detection of Trypanozoon species Acta Trop. 122(2):183–188. (影響因子: 2.3)

余新炳沈繼龍 主編 賴德華 等編者 (2011) 現代病原生物學研究技術 人民衛生出版社 專著

Wang QP, Lai DH, Li Z, Li FJ, Lun ZR (2011). Semicarbazide-sensitive amine oxidase kills African trypanosomes in vitro. Acta Trop. 2011 Feb 117(2): 161–164. (影響因子:2.3 )

Oborník M, Vancová M, Lai DH, Janouškovec J, Keeling PJ, Lukeš J (2011). Morphology and ultrastructure of multiple life cycle stages of the photosynthetic relative of apicomplexa, Chromera velia. Protist 2011 Jan 162(1): 115–130. (影響因子: 3.3)

Lun ZR, Lai DH (共同第一作者), Li FJ, Lukeš J, Ayala FJ. (2010) Trypanosoma brucei: two steps to spread out from Africa. Trends Parasitol. 2010 Sep 26(9): 424–427. (影響因子: 4.9)

Lai DH, Wang QP, Li Z, Lukins AG, Reid SA, Lun ZR. (2010) Investigations into human serum sensitivity expressed by stocks of Trypanosoma brucei evansi. Int. J. Parasitol. 40(6): 705–710. (影響因子: 3.8)

Hashimi H, Benkovičová V, Čermáková P, Lai DH, Horváth A, Lukeš J. (2010) The assembly of F1FO-ATP synthase is disrupted upon interference of RNA editing in Trypanosoma brucei. Int. J. Parasitol. 40(1): 45–54. (影響因子: 3.8)

Lun ZR, Reid SA, Lai DH, Li FJ. (2009) Atypical human trypanosomiasis: a neglected disease or just an unlucky accident? Trends Parasitol. Mar 25(3): 107–108. (影響因子: 4.9)

Lai DH(通訊作者), Wang QP, Li Z, Lukeš J, Lun ZR. (2009) Evolution of the serum resistance-associated SRA gene in African trypanosomes. Chin. Sci. Bull. 54(7):1275–1278. (影響因子: 1.0)

Lai DH, Hashimi H, Lun ZR, Ayala FJ, Lukeš J. (2008) Adaptations of Trypanosoma brucei to gradual loss of kinetoplast DNA: Trypanosoma equiperdum and Trypanosoma evansi are petite mutants of T. brucei. Proc. Natl. Acad. Sci. USA 105(6): 1999–2004. (影響因子: 9.6)

Lai DH, Wang QP, Chen W, Cai LS, Wu ZD, Zhu XQ, Lun ZR. (2008) Molecular genetic profiles among individual Clonorchis sinensis adults collected from cats in two geographic regions of China revealed by RAPD and MGE-PCR methods. Acta Trop. 107(2): 213–216. (影響因子: 2.2)

Wang QP, Lai DH, Zhu XQ, Chen XG, Lun ZR. (2008) Human angiostrongyliasis. Lancet Infect. Dis. Aug 8(10): 621–630. (影響因子: 16.1)

Li FJ, Gasser RB, Lai DH, Claes F, Zhu XQ and Lun ZR. (2007) PCR approach for the detection of Trypanosoma brucei and T. equiperdum and their differentiation from T. evansi based on maxicircle kinetoplast DNA. Mol. Cell. Probes Feb 21(1): 1–7. (影響因子: 1.8)

Li FJ, Lai DH, Lukeš J, Chen XG and Lun ZR. (2006) Doubts about Trypanosoma equiperdum strains classed as Trypanosoma brucei or Trypanosoma evansi. Trends Parasitol. 22(2): 55–56. (影響因子: 4.9)

戴曉俐, 賴德華, 倫照榮. (2006) 四種抗寄生原蟲藥物對伊氏錐蟲、馬媾疫錐蟲和布氏錐蟲感染的治療作用. 熱帶醫學雜誌 6卷12期1240-1244,1248國家核心期刊

Zhu S, Lai DH, Li SQ and Lun ZR. (2006) Stimulative effects of insulin on Toxoplasma gondii replication in 3T3-L1 cells. Cell Bio. Int. Feb 30(2): 149–153. (影響因子: 1.7) SCI E

Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB and Fang YY. (2005) Clonorchiasis: a key foodborne zoonosis in China. Lancet Infect. Dis. 5(1): 31–41. (影響因子: 10.8)