葉麗丹

葉麗丹，博士，浙江大學化學工程與生物工程學院副教授 ，博士生導師。

基本介紹

• 中文名：葉麗丹
• 畢業院校：德國耶拿大學
• 學位/學歷：博士
• 職業：教師
• 專業方向：代謝工程、合成生物學、蛋白質工程
• 任職院校：浙江大學化學工程與生物工程學院

個人經歷

學習經歷：

2006.9-2010.3 德國耶拿大學生物與藥學學院 理學博士

2004.9-2006.6 浙江大學生命科學學院 理學碩士

2000.9-2004.6 浙江大學生命科學學院 理學學士

工作經歷：

2015.1-至今浙江大學化學工程與生物工程學院 副教授

2013.8-2014.12浙江大學化學工程與生物工程學院 講師

2013.2-2013.8 新加坡科技研究局化學與工程科學研究院 Scientist

2010.8-2013.2 新加坡國立大學化學與生物分子工程系 博士後研究員

研究方向

代謝工程

合成生物學

蛋白質工程

主講課程

高年級本科生專業選修課《合成生物學概論》

學術成果

2020:

1.Bing-Mei Su, Ai-Peng Li, Ze-Hui Shao, Muhammad Naeem, Juan Lin*,Li-Dan Ye*, Hong-Wei Yu*. Rational Design of Dehydrogenase/reductases Based on Comparative Structural Analysis of Prereaction-state and Free-state Simulations for Efficient Asymmetric Reduction of Bulky Aryl Ketones. ACS Catalysis,

2019:

1.Muhammad Naeem, Aipeng Li, Muhammad Adnan Younis, Bin Shen,Lidan Ye, Hongwei Yu*. Asymmetric Bioreduction of 4-hydroxy-2-butanone by Carbonyl Reductases PFODH and CpSADH Delivers 1,3-butanediol Enantiomers with Excellent R- and S-enantioselectivity. Biotechnology and Bioprocess Engineering,

2.Ling Jiang,Lidan Ye, Jiali Gu, Weike Su, Wenting Ye. Mechanochemical enzymatic synthesis of 1,4-dihydropyridine calcium antagonists and derivatives. Journal of Chemical Technology and Biotechnology,

3.Xiafen Wu, Shengli Yang*, Hongwei Yu*,Lidan Ye, Bingmei Su, Zehui Shao. Improved enantioselectivity of E. coli BioH in kinetic resolution of methyl (S)-3-cyclohexene-1-carboxylate by combinatorial modulation of steric and aromatic interactions. Bioscience, Biotechnology, and Biochemistry,

4.Wenya Wang*, Sisi Su, Shizhuo Wang,Lidan Ye*, Hongwei Yu*. Significantly improved catalytic efficiency of caffeic acid O-methyltransferase towards N-acetylserotonin by strengthening its interactions with the unnatural substrate's terminal structure.Enzyme and Microbial Technology,

5.Pingping Zhou, Min Li, Bin Shen, Zhen Yao, Qi Bian,Lidan Ye*, and Hongwei Yu. Directed Coevolution of β-Carotene Ketolase and Hydroxylase and Its Application in Temperature-Regulated Biosynthesis of Astaxanthin.Journal of Agricultural and Food Chemistry,

2018:

1.Muhammad Naeem, Ashfaq UrRehman, Bin Shen,Lidan Ye, Hongwei Yu*. Semi-rational engineering of carbonyl reductase YueD for efficient biosynthesis of halogenated alcohols with in situ cofactor regeneration.Biochemical Engineering Journal,

2.Huijuan Cao, Pengyun Huang, Yuxin Yan, Yongkai Shi, Bo Dong, Xiaohong Liu,Lidan Ye, Fucheng Lin, Jianping Lu*.The basic helix-loop-helix transcription factor Crf1 is required for development and pathogenicity of the rice blast fungus by regulating carbohydrate and lipid metabolism: Transcription factor Crf1 in Pyricularia oryzae.Environmental Microbiology,

3.Haiping Jiang, Sujuan Wang, Xuefei Zhou, Liying Wang,Lidan Ye, Zhuxian Zhou, Jianbin Tang, Xiangrui Liu*, Lisong Teng, Youqing Shen. New path to treating pancreatic cancer: TRAIL gene delivery targeting the fibroblast-enriched tumor microenvironment.Journal of Controlled Release,

4.Zhen Yao, Pingping Zhou, Bingmei Su, Sisi Su,Lidan Ye*, Hongwei Yu*. Enhanced isoprene production by reconstruction of metabolic balance between strengthened precursor supply and improved isoprene synthase inSaccharomyces cerevisiae.ACS Synthetic Biology

5.Pingping Zhou, Wenping Xie, Zhen Yao, Yongqiang Zhu,Lidan Ye, Hongwei Yu. Development of a temperature-responsive yeast cell factory using engineered Gal4 as a protein switch.Biotechnology and Bioengineering,

6.Lidan Ye, Chengcheng Yang, Hongwei Yu*. From molecular engineering to process engineering: Development of high-throughput screening methods in enzyme directed evolution.Applied Microbiology and Biotechnology,

2017：

1.Pingping Zhou, Wenping Xie, Aipeng Li, Fan Wang, Zhen Yao, Qi Bian, Yongqiang Zhu, Hongwei Yu,Lidan Ye*. Alleviation of metabolic bottleneck by combinatorial engineering enhanced astaxanthin synthesis in saccharomyces cerevisiae. Enzyme and Microbial Technology,

2.Fan Wang, Xiaomei Lv, Wenping Xie, Pingping Zhou, Yongqiang Zhu, Zhen Yao, Chengcheng Yang, Xiaohong Yang,Lidan Ye*, Hongwei Yu*Combining Gal4p-mediated expression enhancement and directed evolution of isoprene synthase to improve isoprene production inSaccharomyces cerevisiae,Metabolic Engineering,3.Lidan Ye, Xiaomei Lv, Hongwei Yu*Assembly of Biosynthetic Pathways in Saccharomyces cerevisiae Using A Ready-to-use Marker Recyclable Integrative Plasmid Toolbox,Frontiers of Chemical Science and Engineering,

4.Yongqiang Zhu, Wenqiang Lu,Lidan Ye, Zhaofeng Chen, Weijiang Hu, Jianbo Chen, Hongwei Yu*. Enhanced synthesis of Coenzyme Q 10, by reducing the competitive production of carotenoids inRhodobacter sphaeroides.Biochemical Engineering Journal,

5.Yongqiang Zhu,Lidan Ye, Zhaofeng Chen, Weijiang Hu, Yanghui Shi, Jianbo Chen, Chenfei Wang, Yong Li, Weifeng Li, Hongwei Yu*. Synergic Regulation of Redox Potential and Oxygen Uptake to Enhance Production of Coenzyme Q 10, inRhodobacter sphaeroides.Enzyme & Microbial Technology,

6.Xiaohong Yang,Lidan Ye, Aipeng Li, Chengcheng Yang, Huilei Yu, Jiali Gu, Fei Guo, Ling Jiang, Fan Wang, Hongwei Yu*Engineering of D-Fructose-6-Phosphate Aldolase A for Improved Activity towards Cinnamaldehyde,Catalysis Science & Technology

7.Chengcheng Yang,Lidan Ye, Jiali Gu, Xiaohong Yang, Aipeng Li, Hongwei Yu* Directed evolution of mandelate racemase by a novel high-throughput screening method,Applied Microbiology and Biotechnology, 2

2016

1.Lidan Ye, Xiaomei Lv, Hongwei Yu* Engineering microbes for isoprene production.Metabolic Engineering,

2.Xiaomei Lv, Jiali Gu, Fan Wang, Wenping Xie,Min Liu,Lidan Ye*, Hongwei Yu* Combinatorial pathway optimization in Escherichia coli by directed co-evolution of rate-limiting enzymes and modular pathway engineering,Biotechnology and Bioengineering,

3.Weiwei Chen, Hongwei Yu,Lidan Ye* Comparative study on different expression hosts for alkaline phytase engineered inEscherichia coli,Applied Biochemistry and Biotechnology, 2016, 179(6):997-1010

4.Guo Fei,Ye Lidan, Li Aipeng, Yang Xiaohong, Yang Chengcheng,Yu Hongwei*Insight into the Role of Halogen Bond in the Activity of D-Mandelate Dehydrogenase toward Halogenated Substrates,Tetrahedron Letters,

5.Xiaomei Lv, Fan Wang, Pingping Zhou,Lidan Ye, Wenping Xie, Haoming Xu & Hongwei Yu* Dual regulation of cytoplasmic and mitochondrial acetyl-CoA utilization for improved isoprene production inSaccharomyces cerevisiae,Nature Communications

6.Aipeng Li,Lidan Ye, Xiaohong Yang, Bei Wang, Chengcheng Yang, Jiali Gu, Hongwei Yu* Reconstruction of the catalytic pocket and enzyme-substrate interactions enhanced the catalytic efficiency of a short-chain dehydrogenase/reductase,ChemCatChem,

7.Aipeng Li,Lidan Ye, Xiaohong Yang, Chengcheng Yang, Jiali Gu, Yu Hongwei* Structure-guided stereoselectivity inversion of a short-chain dehydrogenase/reductase towards halogenated acetophenones,Chemical Communications,

8.Yan Lyu,Lidan Ye, Jun Xu, Xiaohong Yang, Weiwei Chen, Hongwei Yu*. Recent research progress with phospholipase C fromBacillus cereus.Biotechnology Letters,

2015

1.Lidan Ye, Wenping Xie, Pingping Zhou, Hongwei Yu*. Biotechnological Production of Astaxanthin through Metabolic Engineering of Yeasts,ChemBioEng Reviews,

2.Aipeng Li,Lidan Ye, Hongping Wu, Xiaohong Yang, Hongwei Yu* Characterization of an excellent anti-Prelog short-chain dehydrogenase/reductase EbSDR8 from Empedobacter brevis ZJUY-1401,Journal of Molecular Catalysis B: Enzymatic,

3.Pingping Zhou,Lidan Ye, Wenping Xie, Xiaomei Lv, Hongwei Yu* Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt,Applied Microbiology and Biotechnology,

4.Jiali Gu, Hongfei Tong;Lidan Ye, Hongwei Yu* The role of aromatic residue W20 in the activity and enantioselectivity control of esterase BioH towards aryl substrate,Biochemical Engineering Journal

5.Aipeng Li,Lidan Ye, Fei Guo, Xiaohong Yang, Hongwei Yu *Biocatalytic anti-Prelog reduction of prochiral ketones with whole cells of a newly isolated strain Empedobacter brevis ZJUY-1401,Journal of Molecular Catalysis B: Enzymatic,

6.Wenping Xie, Xiaomei Lv,Lidan Ye, Pingping Zhou, Hongwei Yu* Construction of lycopene-overproducing Saccharomyces cerevisiae by combining directed evolution and metabolic engineering,Metabolic Engineering

7.Wenqiang Lu,Lidan Ye, Xiaomei Lv, Wenping Xie, Jiali Gu, Zhaofeng Chen, Yongqiang Zhu, Aipeng Li, Hongwei Yu*, Identification and elimination of metabolic bottlenecks in the quinone modification pathway for enhanced coenzyme Q10 production in Rhodobacter sphaeroides,Metabolic Engineering

8.Weiwei Chen,Lidan Ye, Fei Guo, Yan Lv, Yu Hongwei*,Enhanced activity of an alkaline phytase fromBacillus subtilis168 in acidic and neutral environments by directed evolution,Biochemical Engineering Journal,

9.Jiali Gu,Lidan Ye,Fei Guo,Xiaomei Lv,Wenqiang Lu,Hongwei Yu*. Improved apparent enantioselectivity of a hydrolase by sequential hydrolysis and racemization,Tetrahedron Letters,

10.Wenping Xie,Lidan Ye, Xiaomei Lv, Haoming Xu, Hongwei Yu*. Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates inSaccharomyces cerevisiae.Metabolic Engineering,

11.Jiali Gu,Lidan Ye,Fei Guo, Xiaomei Lv, Wenqiang Lu, Hongwei Yu*. Rational design of esterase BioH with enhanced enantioselectivity towards methyl (S)-o-chloromandelate.Applied Microbiology and Biotechnology, ,

2014

1.Xiaomei Lv, Wenping Xie, Wenqiang Lu, Fei Guo, Jiali Gu, Hongwei Yu,Lidan Ye* Enhanced isoprene biosynthesis in Saccharomyces cerevisiae by engineering of the native acetyl-CoA and mevalonic acid pathways with a push-pull-restrain strategy.Journal of Biotechnology,

2.Min Liu, Wenping Xie, Haoming Xu, Jiali Gu, Xiaomei Lv, Hongwei Yu,Lidan Ye*. Directed evolution of an exoglucanase facilitated by a co-expressed β-glucosidase and construction of a whole engineered cellulase system inEscherichia coli.Biotechnology Letters,

3.Ling Jiang, Bo Wang, Rongrong Li, Sasa Shen, Hongwei Yu,Lidan Ye*. Catalytic promiscuity ofEscherichia coliBioH esterase: application in the synthesis of 3,4-dihydropyran derivatives.Process Biochemistry,

4.Ling Jiang, Bo Wang, Rongrong Li, Sa Shen, Hongwei Yu,Lidan Ye*. “Amano” Lipase DF-catalyzed efficient synthesis of 2,2’-arylmethylene dicyclohexane-1,3-dione derivatives in anhydrous media.Chinese Chemical Letters

5.Lidan Ye,Mohammad Sufian Bin Hudari, Zhi Li*, Jin Chuan Wu*. Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for L-lactic acid production byBacillus coagulansJI12.Biochemical Engineering Journal,

6.Fei Guo, Stefan Franzen,Lidan Ye, Haoming Xu, Jiali Gu, Hongwei Yu*. Controlling Enantioselectivity of Esterase in Asymmetric Hydrolysis of Aryl Prochiral Diesters by Introducing Aromatic Interactions, Biotechnology and Bioengineering,

7.Rongrong Li, Ling Jiang,Lidan Ye, Jie Lu, Hongwei Yu. Oriented Covalent Immobilization of Esterase BioH on Hydrophilic Modified Fe₃O₄Nanoparticles.Biotechnology and Applied Biochemistry,

8.Jiali Gu, Min Liu, Fei Guo, Wenping Xie, Wenqiang Lu,Lidan Ye, Zhirong Chen, Shenfeng Yuan, Hongwei Yu*.Virtual screening of mandelate racemase mutants with enhanced activity based on binding energy in the transition state.Enzyme and Microbial Technology,

9.Wenqiang Lu,Lidan Ye, Haoming Xu, Wenping Xie, Jiali Gu, Hongwei Yu*.Enhanced production of coenzyme Q10 by self-regulating the engineered MEP pathway inRhodobacter sphaeroides.Biotechnology and Bioengineering,

2013

1.Lidan Ye, Hongna Xu, Hongwei Yu*. Enhanced acylation activity of esterase BioH fromEscherichia coliby directed evolution towards improved hydrolysis activity,Biochemical Engineering Journal,

2.Lidan Ye, Mohammad Sufian Bin Hudari, Xingding Zhou, Dongxu Zhang, Zhi Li*, Jin Chuan Wu*. Conversion of acid hydrolysate of oil palm empty fruit bunch to L-lactic acid by newly isolatedBacillus coagulansJI12,Applied Microbiology and Biotechnology, 2013, 97(11):

3.Lidan Ye, Xingding Zhou, Mohammad Sufian Bin Hudari, Zhi Li*, Jin Chuan Wu*. Highly efficient production of L-lactic acid from xylose by newly isolatedBacillus coagulansC106.Bioresource Technology,

4.Lidan Ye, Hua Zhao, Zhi Li*, Jinchuan Wu*. Improved acid tolerance ofLactobacillus pentosusby error-prone whole genome amplification.Bioresource Technology

5.Xiaolian Wei, Xiawei Jiang,Lidan Ye, Shenfeng Yuan, Zhirong Chen, Min Wu, Hongwei Yu*. Cloning, expression and characterization of a new enantioselective esterase from a marine bacteriumPelagibacterium halotoleransB2T.Journal of Molecular Catalysis B: Enzymatic

6.Xingding Zhou,Lidan Ye, Jin Chuan Wu*. Efficient production of L-lactic acid by newly isolated thermophilicBacillus coagulansWCP10-4 with high glucose tolerance.Applied Microbiology and Biotechnology,

Before 2013

1.Lidan Ye, Jörg Nüske, Dieter Spiteller, René Ullrich, Wilhelm Boland, Gabriele Diekert*.Fluoride- dependent conversion of organic compounds mediated by manganese peroxidases in the absence of Mn(II).Biochemistry,

2.Lidan Ye, Anke Schilhabel, Stefan Bartram, Wilhelm Boland, Gabriele Diekert*. Reductive dehalogenation of brominated ethenes bySulfurospirillum multivoransandDesulfitobacterium hafniensePCE-S.Environmental Microbiolgy,

3.Ji Liu, Hang Min*,Lidan Ye.The negative interaction between the degradation of phenanthrene and tricyclazole in medium, soil and soil/compost mixture, Biodegradation, 2008, 19(5): 695-703

4.葉麗丹，勞佳萍，盧建平，林福呈。球毛殼菌螢光標記與重寄生現象研究。浙江大學學報（農業與生命科學版）,

1. Lidan Ye, Hongwei Yu, Efforts Toward Industrial Biosynthesis of Isoprene. In: Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals (Ed. Sang Yup Lee), Springer International Publishing,

2. Min Liu, Lidan Ye, Hongwei Yu, Directed coevolution of two cellulosic enzymes in Escherichia coli based on their synergistic reactions. In: Protein Engineering Methods and Protocols (Ed. Uwe T. Bornscheuer & Matthias Höhne), Humana Press,