汪凱(重慶醫科大學副研究員):研究方向,主要成就,教育背景,工作經歷,學術任職,獎

汪凱，男，畢業於中國科學院上海巴斯德研究所，理學博士。現就職於重慶醫科大學感染性疾病分子生物學教育部重點實驗室，任副研究員，博士生導師。

基本介紹

中文名：汪凱
國籍：中國
出生地：武漢
學位/學歷：博士
職業：教師

研究方向,主要成就,教育背景,工作經歷,學術任職,獎勵榮譽,科研項目,學術發表,

研究方向

（2）代謝重編程調控肝癌發生髮展的分子機制研究

主要成就

2015年就職於重慶醫科大學，主要從事分子病毒學及肝癌代謝研究；圍繞代謝與信號轉導的互動調控，旨在揭示代謝重編程在肝癌發生髮展中的作用，為肝癌治療提供新的靶點。重醫未來醫學青年創新團隊“病毒感染與代謝重塑研究團隊”負責人，先後主持國家自然科學基金3項；重慶市科委及教委課題2項，重慶醫科大學“新冠肺炎”應急課題等；獲國家發明專利1項。在國內外期刊發表學術論文50餘篇，其中以通訊作者或第一作者（含共同）在Cell Research、Journal of Clinical Investigation、EMBO Journal、Cell Death & Differentiation、Clinical Infectious Diseases、Signal Transduct Target Ther.和Oncogene等期刊發表SCI論文30餘篇，總引用2000餘次，其中3篇為ESI高被引論文，谷歌學術H-index為26。

（1）系統研究了新冠肺炎（COVID-19）患者血清中和抗體反應的動態變化；揭示離子通道蛋白在冠狀病毒的生活周期中發揮了重要作用，有利於進一步揭示冠狀病毒的致病機制，為抗冠狀病毒藥物的研發提供新的藥物靶點；鑑定了一類雙苄四氫異喹啉生物鹼（包括cepharanthine、hernandezine、tetrandrine和neferine等）可明顯抑制冠狀病毒入胞過程。

（2）在動物模型中證實了高果糖攝入促進肝癌的進展，首次揭示了應激環境下腸道菌群衍生的乙酸鹽通過己糖胺代謝重塑調控蛋白質糖基化修飾、促進肝癌進展的新機制，該研究也為添加糖的合理使用和肝癌的防治提供了理論依據和潛在的干預靶點。

（3）發現糖代謝酶PCK1可促進癌基因甲基化，抑制肝細胞癌進展。

教育背景

2013.7-2015.7 中國科學院上海生命科學研究院生物學博士後

2006.9-2012.1 中國科學院上海巴斯德研究所微生物學理學博士

2002.9-2006.7 新疆大學生命科學與技術學院生物科學理學學士

工作經歷

2020.10- 重慶醫科大學感染性疾病分子生物學重點實驗室副研究員

2019.10-2020.10 重慶醫科大學感染性疾病分子生物學重點實驗室副教授

2015.9-2019.9重慶醫科大學基礎醫學院病原生物學教研室講師

2012.5-2013.5 中科院上海巴斯德研究所分子病毒與免疫重點實驗室助理研究員

學術任職

中國抗癌協會腫瘤代謝專委會青年委員；

重慶抗癌協會化療專業委員會委員；

Advanced Science、Nat Commun、Clin Transl Med、Genes & Disease等學術期刊審稿人。

獎勵榮譽

2021年重慶醫科大學“優秀科技成果獎”

2021年愛思唯爾Elsevier“中國金色開放獲取高下載論文學者”

2021年第二十一屆重慶市期刊優秀論文年度優秀論文一等獎

2023年 MCE中國生命科學研究促進獎-“春華”季優質科研獎

2023年川渝科技學術大會優秀論文特等獎 (排名第三)

科研項目

PCK1通過降低O-GlcNAc糖基化修飾抑制肝癌轉移的機制研究
糖異生代謝酶PCK1上調組蛋白H3K9me3修飾抑制肝癌的機制研究

學術發表

Rui Liu, Dongmei Gou, Jin Xiang, Xuanming Pan, Qingzhu Gao, Peng Zhou, Yi Liu, Jie Hu, Kai Wang*, Ni Tang*, O-GlcNAc modified-TIP60/KAT5 is required for PCK1 deficiency-induced HCC metastasis. Oncogene, 2021, doi: 10.1038/s41388-021-02058-z (*Co-corresponding author)
Jin Xiang, Chang Chen, Rui Liu, Dongmei Gou, Lei Chang, Haijun Deng, Qingzhu Gao, Wanjun Zhang, Lin Tuo, Xuanming Pan, Li Liang, Jie Xia, Luyi Huang, Ke Yao, Bohong Wang, Zeping Hu, Ailong Huang*, Kai Wang*, Ni Tang*, Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation. Journal of Clinical Investigation, 2021, 131(8):e144703. (*Co-corresponding author)
Pai Peng, Hai-jun Deng, Jie Hu, Xiao-yu Wei, Jian-jiang Xue, Ting-ting Li, Liang Fang, Bei-zhong Liu, Ai-shun Jin, Feng-li Xu, Kang Wu, Quan-xin Long, Juan Chen, Kai Wang*, Ni Tang*, Ai-long Huang*, Humoral responses in naive or SARS-CoV-2 experienced individuals vaccinated with an inactivated vaccine. Cell Discovery, 2021, 7:68. (*Co-corresponding author)
Kai Wang, Quan-Xin Long, Hai-Jun Deng, Jie Hu, Qing-Zhu Gao, Gui-Ji Zhang, Chang-long He, Lu-Yi Huang, Jie-li Hu, Juan Chen*, Ni Tang*, Ai-Long Huang*, Longitudinal Dynamics of the Neutralizing Antibody Response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection. Clinical Infectious Diseases, 2021. 73(3):e531-e539. (Co-first author)
Chang-Long He, Lu-Yi Huang, Kai Wang, Chen-Jian Gu, Jie Hu, Gui-Ji Zhang, Wei Xu, You-Hua Xie*, Ni Tang*, Ai-Long Huang*, Identification of bis-benzylisoquinoline alkaloids as SARS-CoV-2 entry inhibitors from a library of natural products. Signal Transduction and Targeted Therapy, 2021, doi : 10.1038/s41392-021-00531-5(#Co-first author)
Jie Hu, Pai Peng, Kai Wang, Liang Fang, Fei-yang Luo, Ai-shun Jin, Bei-zhong Liu*, Ni Tang*, Ai-long Huang*, Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies. Cellular & Molecular Immunology, 2021.(#Co-first author)
Pai Peng, Jie Hu, Hai-jun Deng, Bei-zhong Liu, Liang Fang, Kai Wang*, Ni Tang*, Ai-long Huang*, Changes of humoral immunity response in SARS-CoV-2 convalescent patients over 8 months. Cellular & Molecular Immunology, 2021, 18(2), 490–491. (*Co-corresponding author)
Jie Hu, Qingzhu Gao, Changlong He, Ailong Huang, Ni Tang*, Kai Wang*, Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2. Genes & Diseases, 2020, 7(4):551-557. (*Co-corresponding author)
Jie Hu, Qingzhu Gao, Yang Yang, Jie Xia, Wanjun Zhang, Yao Chen, Zhi Zhou, Lei Chang, Yuan Hu, Hui Zhou, Li Liang, Xiaosong Li, Quanxin Long, Kai Wang*, Ailong Huang*, Ni Tang*, Hexosamine biosynthetic pathway promotes the antiviral activity of SAMHD1 by enhancing O-GlcNAc transferase-mediated protein O-GlcNAcylation. Theranostics, 2021, 11(2):805-823. (*Co-corresponding author)
Qingzhu Gao, Guiji Zhang, Yaqiu Zheng, Yi Yang, Chang Chen, Jie Xia, Li Liang, Chong Lei, Yuan Hu, Xue-Fei Cai, Wenlu Zhang, Hua Tang, Yaxi Chen, Ailong Huang*, Kai Wang*, Ni Tang*, SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC. Cell Death & Differentiation, 2020, 27(3):1086–1104. (*Co-corresponding author)
Guiji Zhang, Xia Tang, Li Liang, Wanfeng Zhang, Dewei Li, Xiaoyuan Li, Dachun Zhao, Yaqiu Zheng, Yanhong Chen, Bingtao Hao, Kai Wang*, Ni Tang*, Keyue Ding*, DNA and RNA sequencing identified a novel oncogene VPS35 in liver hepatocellular carcinoma, Oncogene, 2020, 39(16):3229-3244. (*Co-corresponding author)
Jin Xiang, Yuhong Zhang, Lin Tuo, Rui Liu, Dongmei Gou, Li Liang, Chang Chen, Jie Xia, Ni Tang, Kai Wang*,Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-Seq. Genes & Diseases, 2020,7(1):150-159. (Corresponding author)
Jingjing Li, Qiujie Wang, Yi Yang, Chong Lei, Fan Yang, Li Liang, Chang Chen, Jie Xia, Kai Wang*, Ni Tang*, GSTZ1 deficiency promotes hepatocellular carcinoma proliferation via activation of the KEAP1/NRF2 pathway. Journal of Experimental & Clinical Cancer Research, 2019, 38(1):438.(*Co-corresponding author)
Fan Yang, Jingjing Li, Haijun Deng, Yihao Wang, Chong Lei, Qiujie Wang, Jin Xiang, Li Liang, Jie Xia, Xuanming Pan, Xiaosong Li, Quanxin Long, Lei Chang, Ping Xu, Ailong Huang*, Kai Wang*, Ni Tang*, GSTZ1-1 Deficiency Activates NRF2/IGF1R Axis in HCC via Accumulation of Oncometabolite Succinylacetone. The EMBO Journal, 2019, 38(15):e101964. (*Co-corresponding author)
Xuemei Chen, Yuan Hu, Wenlu Zhang, Ke Chen, Jie Hu, Xiaosong Li, Li Liang, Xuefei Cai, Jieli Hu, Kai Wang*, Ailong Huang*, Ni Tang*, Cisplatin induces autophagy to enhance hepatitis B virus replication via activation of ROS/JNK and inhibition of the Akt/mTOR pathway. Free Radical Biology and Medicine, 2019, 131: 225-236. (*Co-corresponding author)
Lin Tuo, Jin Xiang, Xuanming Pan, Jieli Hu, Hua Tang, Li Liang, Jie Xia, Yuan Hu, Wenlu Zhang, Ailong Huang*, Kai Wang*, Ni Tang*, PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27 axis. Journal of Experimental & Clinical Cancer Research, 2019, 38(1):50.(*Co-corresponding author)
Lin Tuo, Jin Xiang, Xuanming Pan, Qingzhu Gao, Guiji Zhang, Yi Yang, Li Liang, Jie Xia, Kai Wang*, Ni Tang*, PCK1 Downregulation Promotes TXNRD1 Expression and Hepatoma Cell Growth via the Nrf2/Keap1 Pathway. Frontiers in Oncology, 2018. (*Co-corresponding author)
Kai Wang, Wei Lv, Jianfei Chen, Shiqi Xie, Hongyan Shi, Haojen Hsu, Wenjing Yu, Ke Xu, Chao Bian, Wolfgang B Fischer, Wolfgang Schwarz, Li Feng*, Bing Sun*, PEDV ORF3 encodes an ion channel protein and regulates virus production. FEBS Letters, 2012.
Kai Wang, Shiqi Xie, Bing Sun*. Viral proteins function as ion channels. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2011.
Shiqi Xie, Kai Wang, Wenjing Yu, Wei Lv, Ke Xu, Jianwei Wang, Bin Ye, Wolfgang Schwarz, Qi Jin*, Bing Sun*, DIDS blocks a chloride-dependent current that is mediated by the 2B protein of enterovirus 71, Cell Research, 2011.(#Co-first author)
Ronghua Zhang, Kai Wang, Wei Lv, Wenjing Yu, Shiqi Xie, Ke Xu, Wolfgang Schwarz, Sidong Xiong*, Bing Sun*, The ORF4a protein of human coronavirus 229E functions as a viroporin that regulates viral production. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2014. (#Co-first author)
Ronghua Zhang, Kai Wang, Xianqiang Ping, Wenjing Yu, Zhikang Qian, Sidong Xiong*, Bing Sun*, The ns12. 9 accessory protein of human coronavirus OC43 is a viroporin involved in virion morphogenesis and pathogenesis. Journal of Virology, 2015.
Silvia Schwarz, Kai Wang, Wenjing Yu, Bing Sun, Wolfgang Schwarz*, Emodin inhibits current through SARS-associated coronavirus 3a protein. Antiviral Research, 2011.
Silvia Schwarz, Daniel Sauter, Kai Wang, Ronghua Zhang, Bing Sun, Anastasia Karioti, Anna Rita Bilia, Thomas Efferth, Wolfgang Schwarz*, Kaempferol derivatives as antiviral drugs against the 3a channel protein of coronavirus. Planta medica, 2014.
Daniel Sauter, Silvia Schwarz, Kai Wang, Ronghua Zhang, Bing Sun, Wolfgang Schwarz*, Genistein as antiviral drug against HIV ion channel. Planta medica, 2014.
Jie Hu, Xiao-yu Wei, Jin Xiang, Pai Peng, Feng-li Xu, Kang Wu, Fei-yang Luo, Ai-shun Jin, Liang Fang, Bei-zhong Liu*, Kai Wang*, Ni Tang*, Ai-Long Huang*, Reduced neutralization of SARS-CoV-2 B.1.617 variant by convalescent and vaccinated sera. Genes & Diseases, 2022.
Qian Ye, Yi Liu, Guiji Zhang, Haijun Deng, Xiaojun Wang, Lin Tuo, Chang Chen, Xuanming Pan, Kang Wu, Jiangao Fan, Qin Pan, Kai Wang*, Ailong Huang*, Ni Tang*, Deficiency of gluconeogenic enzyme PCK1 promotes metabolic-associated fatty liver disease through PI3K/AKT/PDGF axis activation in male mice. Nature Communications, 2023.
Dongmei Gou, Rui Liu, Xiaoqun Shan, Haijun Deng, Chang Chen, Jin Xiang, Yi Liu, Qingzhu Gao, Zhi Li, Ailong Huang*, Kai Wang*, Ni Tang*, Gluconeogenic enzyme PCK1 supports S-adenosylmethionine biosynthesis and promotes H3K9me3 modification to suppress hepatocellular carcinoma progression. Journal of Clinical Investigation, 2023.
Peng Zhou, Wen-yi Chang, De-ao Gong, Jie Xia, Wei Chen, Lu-yi Huang, Rui Liu, Yi Liu, Chang Chen, Kai Wang*, Ni Tang*, Ai-long Huang*, High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate. Cell Metabolism, 2023.

汪凱(重慶醫科大學副研究員)