李劍峰，男，博士，中山大學生命科學學院教授、博士生導師。
基本介紹
- 中文名：李劍峰
- 職業：教師
- 畢業院校：中山大學
- 學位/學歷：博士
- 專業方向：生物學、植物學
- 職務：博士生導師
- 職稱：教授
研究方向,人物經歷,講授課程,學術成就,承擔課題,論文專著,
研究方向
- 植物免疫信號轉導（主要研究方向）
- 植物靶向基因調控技術
人物經歷
1995年免試進入中山大學生化專業攻讀本科。
1999年免試進入中山大學生化與分子生物學專業攻讀碩士。
2002年起在中山大學生化與分子生物學專業攻讀博士，於2005年獲博士學位。
2003-2004年在香港科技大學做訪問學者（Visiting Scholar）。
2005.11-2008.10在美國田納西大學做博士後研究員(Postdoctoral Associate)。
2008.11-2014.8在美國哈佛大學醫學院/麻薩諸塞州綜合醫院任助理研究員（Research Fellow）。
2014年9月起任中山大學生命科學學院教授，博士生導師。
講授課程
目前主要講授《生命科學史》。
學術成就
目前以第一作者或通訊作者在Nature, Nature Biotechnology，Nature Plants，Cell Host & Microbe，Plant Cell（2篇）等SCI期刊上發表論文23篇；第一作者或通訊作者專著6篇；目前擔任植物分子生物學專業期刊Plant Methods（影響因子：4.269）的Associate Editor，擔任Nature Biotechnology，Nature Plants，Nature Communications, PLoS Pathogens，Molecular Plant,Plant Physiology等知名SCI期刊的審稿人 ，德國教育科學部研究基金（BMBF）和義大利fondazione cariplo研究基金海外評審專家。入選2014年中山大學百人計畫“青年傑出人才”，2015年國家優秀青年科學基金獲得者。
承擔課題
- 中山大學百人計畫科研啟動項目（2015.1-2016.12）：植物免疫信號轉導
- 國家自然科學基金優青基金（2016.1-2018.12）：植物免疫識別與信號轉導
- 國家自然科學基金面上項目（2016.1-2019.12）：擬南芥CDG1負調控免疫信號轉導中MAPK激活的分子機制研究
- 國家自然科學基金面上項目（2018.1-2021.12）：擬南芥天生免疫中GPA1調控細菌鞭毛信號轉導的分子機制研究
論文專著
第一作者及通訊作者論文（按時間倒序）
Zhang N, Zhang D, Chen SL, Gong BQ, Guo Y, Xu L, Zhang XN, Li JF* (2018) Engineering artificial microRNAs for multiplex gene silencing and simplified transgenic screen. Plant Physiology，178: 989-1001. (*通訊作者)
Liu J, Liu B, Chen S, Gong BQ, Chen L, Zhou Q, Xiong F, Wang M, Feng D, Li JF*, Wang HB*, Wang J* (2018) A tyrosine phosphorylation cycle regulates fungal activation of a plant receptor Ser/Thr kinase. Cell Host & Microbe，23: 241-253. (*並列通訊作者)
Li Z, Zhang D, Xiong X, Yan B, Xie W, Sheen J, Li JF* (2017) A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants, 3: 930-936. (*通訊作者)
Xu L, Yao X, Zhang N, Gong BQ, Li JF* (2017) Dynamic G protein alpha signaling in Arabidopsis innate immunity. Biochemical and Biophysical Research Communications, pii: S0006-291X(17)31382-31387. (*通訊作者)
Gong BQ, Xue J, Zhang N, Xu L, Yao X, Yang QJ, Yu Y, Wang HB, Zhang D, Li JF* (2017) Rice chitin receptor OsCEBiP is not a transmembrane protein but targets to plasma membrane via a GPI anchor. Molecular Plant, 10: 767-770. (*通訊作者)
Zhang D, Li Z, Yan B and Li JF* (2016) A novel RNA-guided RNA-targeting CRISPR tool. Science China-Life Sciences, 59:854-856.(*通訊作者) (Invited review)
Zhang D and Li JF* (2016) DNA-guided genome editing tool. Science China-Life Sciences, 59: 740-741. (*通訊作者) (Invited review)
Zhang D, Li Z and Li JF* (2016) Targeted gene manipulation in plants using the CRISPR/Cas technology. Journal of Genetics and Genomics, 43: 251-262. (*通訊作者) (Invited review)
Zhang D, Li Z and Li JF* (2015) Genome editing: New antiviral weapon for plants. Nature Plants, 1: 15146. (*通訊作者) (Invited review)
Cheng ZY#, Li JF#, Niu Y, Zhang XC, Woody OZ, Xiong Y, Djonovic S, Millet Y, Bush J, McConkey BJ, Sheen J, Ausubel FM (2015) Pathogen-secreted proteases activate a novel plant immune pathway. Nature, 521: 213-216. (#並列第一作者)
Li JF*, Zhang D, Sheen J (2014) Epitope-tagged protein-based artificial microRNA screens for optimized gene silencing in plants. Nature Protocols, 9: 939-949. (*通訊作者) (Invited paper)
Li JF, Norville JE, Aach J, McCormack M, Zhang DD, Bush J, Church GM, Sheen J (2013) Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nature Biotechnology, 31: 688-691.
Li JF*, Chung HS, Niu Y, Bush J and Sheen J (2013) Comprehensive artificial microRNA screens for effective gene silencing in plants. The Plant Cell, 25: 1507-1522. (*通訊作者)
Liu B#, Li JF#, Ao Y#, Qu J, Li Z, Su J, Zhang Y, Liu J, Feng D, Qi K, He Y, Wang J and Wang HB (2012) Lysin motif-containing proteins LYP4 and LYP6 play dual roles in peptidoglycan and chitin perception in rice innate immunity. The Plant Cell, 24: 3406-3419. (#並列第一作者)
Li JF*, Bush J, Xiong Y, Li L and McCormack M (2011) High-throughput protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS ONE,6: e27364. (*通訊作者)
Li JF*, Li L and Sheen J (2010) A rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology. Plant Methods, 6:1. (*通訊作者)
Li JF*, Park E, von Arnim AG and Nebenführ A* (2009) The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods, 5:6. (*通訊作者)
Li JF* and Nebenführ A (2008) Interdependence of dimerization and organelle binding in myosin XI. The Plant Journal, 55:478-490. (*通訊作者)
Li JFand Nebenführ A (2008) The tail that wags the dog: the globular tail domain defines the function of myosin V/XI. Traffic, 9:290-298.
Li JFand Nebenführ A (2007) Organelle targeting of myosin XI is mediated by two globular tail subdomains with separate cargo binding sites. Journal of Biological Chemistry, 282:20593-20602.
Li JF, Qu LH and Li N (2005) Tyr152 plays a central role in catalysis of 1-aminocyclopropane-1-carboxylate synthase. Journal of Experimental Botany, 56:2203-2210.
Li JF, Qi R, Qu LH, Mattoo AK and Li N (2005) Cleavages of the carboxyl-terminus of LeACS2, a tomato ACC synthase isomer, by a 64-kDa tomato metalloprotease produce a truncated but active enzyme. Journal of Integrative Plant Biology, 47:1352-1363.
Li JF, Qu LH and Li N (2005) Isolation of a tomato protease that may be involved in proteolysis of 1-aminocyclopropane-1-carboxylate synthase. Journal of Integrative Plant Biology, 47:1220-1227.
第一作者或通訊作者論著
Zhang N, Zhang D, Li JF* (2016) A simple protoplast-based method for screening potent artificial miRNA for maximal gene silencing in Arabidopsis. Current Protocols in Molecular Biology, 117: 26.9.1-26.9.9. (*通訊作者)
Li JF*, Zhang D and Sheen J (2015) Targeted plant genome editing via the CRISPR/Cas9 technology. Methods in Molecular Biology, 1284:239-255. (*通訊作者)
Li JF* and Zhang D (2014) Quantitative protein-protein interaction analysis by split firefly luciferase complementation in plant protoplasts. Current Protocols in Molecular Biology, 107: 20.9.1-20.9.8. (*通訊作者)
Li JF*, Zhang D and Sheen J (2014) Cas9-based genome editing in Arabidopsis and Tobacco. Methods in Enzymology, 546:459-472. (*通訊作者)
Li JF* and Sheen J (2012) DNA purification from multiple sources in plant research with homemade silica resins. Methods in Molecular Biology, 862:53-59. (*通訊作者)
Li JFand Nebenführ A (2010) FAST technique for Agrobacterium-mediated transient gene expression in seedlings of Arabidopsis and other plant species. Cold Spring Harbor Protocols, 5: pdb.prot5428.
