曾文先

人物經歷

1984年本科畢業於四川農學院，1987年碩士研究充樂良生畢業於四川農業大學。1996年10月至2001年3月在日本國立廣島大學留學，獲博士學位。

1987.07 – 1991.11 助教，四川農業大學畜牧系駝淋企檔

1991.12 – 1995.11 講師，四川農業大學動物科技學院

1995.12 – 2009.06 副教授，四川農業大學動物科技學院

2001.04 – 2003.04 博士後，日本廣島大學，美國杜蘭大學細胞及分子生物學系

2003.04 – 2009.08 博士後,特別研究員，美國賓夕法尼亞大學生殖細胞及轉基因動物研究中心

2009.09．至今教授博士生導師，西北農林科技大學動物科技學院

2015.08-2016.02 特聘教授，日本廣島大學生物圈研究科

研究領域

1. 轉基因技術：利用CRISPR/Cas9 技術編輯基因；建立和完善利用精原幹細胞製作轉基因動物的新技術。

2. 動物生殖細胞工程：主要研究精原幹細胞的增殖與分化的表觀調控機理。

重點研究非編碼RNA（microRNA，piRNA，long-noncoding RNA等）對精原幹細胞的增殖與分化的影響及其作用機理；精市笑腿研究DNA甲基化及組蛋白甲基化表觀修飾調節精原幹細胞增殖與分化的分子機理。

3. 精液保存：主要研究在體外過程中豬精子受到傷害的機理，最佳化和完善精子保存配方，建立豬精子保存的新技術。　

學術兼職

中國細疊棵駝胞生物學學會理事

陝西省細胞生物學會副理事長

中國動物學會繁殖生物學學會理事

中國動物生物工程學會動物生物技術分會理事

中國畜牧獸醫學會動物繁殖分會理事

中國畜牧獸醫學會養羊分會理事　

科研課題

國家重點研發計畫項目：“高產種豬高效安全養殖技術套用與示範” （主持課題一“標準化豬人工授精技術體系構建”，執行時間2018.06-2020.12年，項目的編號2018YFD0501000）（225萬）

國家重點基礎研究發展計畫（格棄973計畫）項目課題： “精子發生成熟的遺傳和表觀遺傳調控研究”，子匙棕旬課題主持人；（執行時間2014-2018年, 批准號2014CB943100）敬鴉墓（104萬）

主持國家自然科學基金面向項目：豬性原細胞向精原幹細胞轉化的分子機制及其體外長期培養體系的建立（2011-2013年，批准號31072029）（35萬）

主持國家自然科學基金面向項目：豬精原幹細胞增殖與分化的表觀修飾調節機理（2013-2016年，批准號31272439）（80萬）

主持國家自然科學基金面向項目：mRNA的m6A甲基化修飾對精原幹細胞增殖與分化的調控作用與機制研究（2016-2019年，批准號31572401）（60萬）

主持國家自然科學基金面向項目：組蛋白甲基化酶SETDB1對豬精原幹細胞增殖與分化的調控作用及其機制研究（2018-2021年,批准號31772605）（60萬）

國家自然科學基金重點項目：人精原幹細胞自我更新與分化為精子細胞的分子機理研究（2013-2017年，批准號31230048）（30萬）

主持教育部博士點基金項目（博導類）：“甲基化修飾對豬精原幹細胞的自我更新與分化的調節作用” （執行時間2014-2016年，批准號20130204110017）（12萬）

主持陝西省“百人計畫”科研基金，“精原幹細胞增殖與分化的機理”， 2010-2013 （50萬）

主持西北農林科技大學2015年度試驗示範站(基地)科技成果推廣專項：肉羊多羔性狀早期選育技術研究與示範（TGZX2015-33）（7萬）

主持西北農林科技大學引進人才科研啟動基金（Z111020902）（200萬）　

主要論文

(* 通訊作者)

HuangT,Gao Q,Feng T,Zheng Y,Guo J,Zeng W*. FTOKnockout Causes Chromosome Instability and G2/M Arrest in Mouse GC-1 Cells. Front Genet.2019 Jan 21;9:732. doi: 10.3389/fgene.2018.00732. eCollection 2018. (IF: 4.141)

Wen J, Lv Y, Xu Y, Zhang P, Li H, Chen X, Li X, Zhang L, Liu F*, Zeng W* and Sun S*. Construction of a biodegradable, versatile nanocarrier for optional combination cancer therapy. Acta Biomater.2019 Jan 1;83:359-371. doi: 10.1016/j.actbio.2018.11.009. Epub 2018 Nov 7. (IF: 6.383)

Zhu Z; Li R, Ma G, Bai W, Fan X, Lv Y, Lou J, Zeng W*. 5’-AMP-activated protein kinase regulates goat sperm functions via energy metabolism in vitro. Cellular Physiology and Biochemistry. 2018;47(6):2420-2431. doi: 10.1159/000491616. Epub 2018 Jul 10. (IF=″5.562)

Wang X,Zhang P,Li L,Che D,Li T,Li H,Li Q,Jia H,Tao S,Hua J,Zeng W,LiaoM. miRNA editing landscape reveals miR-34c regulated spermatogenesis through structure and target change in pig and mouse. Biochem Biophys Res Commun.2018 Aug 25;502(4):486-492. doi: 10.1016/j.bbrc.2018.05.197. Epub 2018 Jun 5. (IF: 2.559)

Deng Z, Zhang S, Gu S, Ni X, Zeng W*, Li X*. Useful Bicistronic Reporter System for Studying Poly(A) Site-Defining cis Elements and Regulation of Alternative Polyadenylation. International Journal Molecular Science. 2018, 19, 279; doi:10.3390/ijms19010279 （IF=3.73）

Lv Y, Zhang P, Guo J, Zhu Z, Li X, Xu D, Zeng W*. Melatonin protects mouse spermatogonial stem cells against hexavalent chromium-induced apoptosis and epigenetic histone modification. Toxicology & Applied Pharmacology. 2018;340:30-38. doi:10.1016/j.taap.2017.12.017 （IF=″3.791）

Zhang P, Chen X, Zheng Y, Zhu J, Qin Y, Lv Y, Zeng W*. Phospholipase D family member 6 (PLD6) is a surface marker for enrichment of undifferentiated spermatogonia in pre-pubertal boars. Stem cell & Development. 2018; 27(1):55-64 doi: 10.1089/scd.2017.0140. Epub 2017 Dec 6. (IF=″3.562)″

Chang Y#, Lu Y#, Wei P, Zhang P, Pu L, Chen X, Yang K, Li X, Lu Y, Hou C, Pei Y*, Zeng W*, Pei Z*. Multifunctional glyco nanofibers: siRNA induced supermolecular assembly for co-delivery in vivo. Adv Funct Mater 2017; 27:1703083. doi/10.1002/adfm.201703083/full (#共同一作, IF=″12.124)

Liu T, Chen X, Li T, Li X, lyu Y, Fan X, Zhang P, Zeng W*. Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 2017:1860(10):1094-1102 (IF=″5.018)

Chen X, Che D, Zhang P, Li X, Yuan Q, Liu T, Guo J, Feng T, Wu L, Liao M, He Z, Zeng W*. Profiling of miRNAs in porcine germ cells during spermatogenesis. Reproduction. 2017;154 (6) 789-798, doi: 10.1530/REP-17-0441. (IF=″3.10)

Zhang P, Chen X, Zheng Y, Zhu J, Qin Y, Lv Y, Zeng W*. Long-term propagation of porcine undifferentiated spermatogonia. Stem cell & Development. 2017; 26(15): 1121-1131. (IF=″3.562)

Zhu Z; Ren Z, Fan X, Pan Y, Lv S, Pan C, Lei A, Zeng W*. Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages. PLoS One. 2017; 12(7): e0181110. Published online 2017 Jul 10. doi: 10.1371/journal.pone.0181110.(IF=″2.806)

Zhu Z, Fan X, Pan Y, Lu Y, Zeng W*. Trehalose improves rabbit sperm quality during cryopreservation. Cryobiology. 2017; 75:45-51. DOI.10.1016/j.cryobiol.2017.02.006. (IF=″1.996)

Zhu Z, Fan X, Lv Y, Lin Y, Wu D, Zeng W*. Glutamine protects rabbit spermatozoa against oxidative stress via glutathione synthesis during cryopreservation. Reproduction, Fertility and Development. 2017；29(11):2183-2194.. DOI: 10.1071/RD17020. (IF=″2.656)

Liu T, Zhang P, Li T, Chen X, Zhu Z, Lyu Y, Li X, Tian X, Zeng W*. SETDB1 plays an essential role in maintenance of gonocyte survival in pigs. Reproduction. 2017; 154:23-34. doi: 10.1530/REP-17-0107. (IF=″3.10)

Chen X, Li X, Guo J, Zhang P, Zeng W*. The roles of microRNAs in regulation of mammalian spermatogenesis. Journal of Animal Science and Biotechnology. 2017; 8: 35; 779-786. doi: 10.1186/s40104-017-0166-4. (IF=″2.05)

Yu S, Zhang P, Dong W, Zeng W, Pan C. Identification of stem leydig cells derived from pig testicular interstitium. Stem Cells International. 2017; 2740272.doi: 10.1155/2017/2740272. (IF=″3.54)

Zeng W, Alpaugh W, Stefanovski D, Schlingmann K, Dobrinski I and Turner RM. Xenografting of isolated equine (Equus caballus) testis cells results in de novo morphogenesis of seminiferous tubules but not spermatogenesis. Andrology. 2017; 5:336-47. doi: 10.1111/andr.12308. (IF=″2.43)

Umehara T, Kawashima I, Kawai T, Hoshino Y, Morohashi KI, Shima Y, Zeng W, Richards JS, Shimada M. Neuregulin 1 Regulates Proliferation of Leydig cells to support spermatogenesis and sexual behavior in adult mice. Endocrinology. 2016;157:4899-4913. (IF=″4.16)

An J，Qin J， Wan Y，Zhang Y，Hu Y，Zhang C，Zeng W*. Histone lysine methylation exhibits a distinct distribution during spermatogenesis in pigs. Theriogenology. 2015; 84:1455-62. (IF=″1.80)

Qin J，Xu H，Zhang P，Zhang C，Zhu Z，Qu R，Qin Y，Zeng W*. An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo. Journal of Animal Science and Biotechnology. 2015; 6: 59. (IF=″1.68)

Zhu Z, Fan X, Lv Y, Zhang N, Fan C, Zhang P, Zeng W*. Vitamin E analogue improves rabbit sperm quality during the process of cryopreservation through its antioxidative action. PloS One 2015; 10.e0145383. (IF=″3.23)

Xu H, Qin J, Zhang K, Zeng W*. Dynamic expression profile of DNA methyltransferases in rat testis development. Polish Journal of Veterinary Sciences. 2015; 18:549-556.（IF=″0.60）

An J, Zhang X, Qin J, Wan Y, Hu Y, Liu T, Li J, Dong W, Du E, Pan C, Zeng W*. The histone methyltransferase ESET is required for the survival of spermatogonial stem/progenitor cells in mice. Cell Death & Disease. 2014; 5, e1196; doi:10.1038/cddis.2014.171（IF=″5.18）

Zheng Y, Zhang Y, Qu R, He Y, Tian X，Zeng W*. Spermatogonial Stem Cells from Domestic Animals: Progress and Prospects. Reproduction. 2014; 147:R65-74 (doi: 10.1530/REP-13-0466).（IF=″3.26）

Zheng Y, He Y, An J, Qin J, Zhang Y, Tian X, Zeng W*. THY1 is a surface marker of porcine gonocytes. Reproduction, Fertility and Development. 2014; 26:533-9. (doi: 10.1071/RD13075). （IF=″2.58）

Zeng W, Tang L, Bondareva A, Luo J, Megee SO, Modelski M, Blash S, Melican DT, Destrempes MM, Oerton SA, Gavin WG, Ayres S, Echelard Y, Dobrinski I. Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs. Biology of Reproduction. 2013; 88(1):27,1-9 (doi: 10.1095/biolreprod.112.104422). （IF= 4.03）

He Z, Jiang J, Kokkinaki M, Tang L, Zeng W, Gallicano I, Dobrinski I, Dym M. MiRNA-20 and MiRNA-106a Regulate Spermatogonial Stem Cell Renewal at the Post-transcriptional Level via Targeting TAT3 and Ccnd1. Stem Cells. 2013 (doi: 10.1002/stem.1474) （IF= 7.701）

Zheng Y, Tian X, Zhang Y, Qin J, An J, Zeng W*. In vitro propagation of male germline stem cells from piglets. Journal of Assisted Reproduction and Genetics. 2013; 30:945-52. (doi: 10.1007/s10815-013-0031-0) (IF=″2.16)

Xu H, Xu Y, Liang X, Wang Y, Jin F, Liu D, Ma Y, Yuan H, Song X, Zeng W*. Porcine skeletal muscle differentially expressed gene ATP5B: molecular characterization, expression patterns, and association analysis with meat quality traits. Mammalian Genome. 2013;24:142-50. (doi: 10.1007/s00335-013-9446-2) （IF= 2.42）

Lu Y, Xu D, Zhou J, Ma Y, Jiang Y, Zeng W*，Dai W*. Differential responses to genotoxic agents between induced pluripotent stem cells and tumor cell lines, Journal of Hematology & Oncology 2013, 6:71.(doi: 10.1186/1756-8722-6-71) （IF= 4.46）

Zeng W, Tang L, Bondareva A, Luo J, Blash S, Melican DT, Destrempes MM, Overton SA, Modelski M, Megee SM, Gavin WG , Ayres S, Echelard Y, Dobrinski I. Non-viral transduction of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation. Molecular Reproduction and Development 2012; 79:255-261 (doi/10.1002/mrd.22014/pdf)（第一，二作者貢獻相同）（IF= 2.53）

Zeng W, Dobrnski I. Identification of spermatogonia by labeling for UCH-L1 in whole mounted seminiferous tubules. Molecular Reproduction and Development. 2012; 79:161. （IF=″2.53）

Turner RM. Zeng W. The Emerging pathophysiology of age-related testicular degeneration with a focus on the stallion and an update on potential therapies. Reproduction in Domestic Animals. 2012; 47(suppl.4) :178-86（IF= 1.36）

Zeng W, Baumann C, Schmidtmann A, Honaramooz A, Tang L, Bondareva A, Dores C, Fan T, Xi S, Geiman T, Rathi R, de Rooij D, De La Fuente R, Muegge K, Dobrnski I. Lymphoid-Specific Helicase (HELLS) is essential for meiotic progression in mouse. Biology of Reproduction. 2011; 84：1235-41. （IF= 3.87）

Turner R, Zeng W, Li Y, Dobrinski I. Paracrine factors from normal equine testicular tissue improve the condition of xenografts of degenerate testicular tissue. Animal Reproduction Science.2010;22:673-83. （IF= 1.56）

Turner R, Rathi R, Honaramooz A, Zeng W, Dobrinski I. Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus). Reproduction, Fertility and Development. 2010;221:673-83. (IF=″2.66)

Zeng W, Megee SO, Snedaker AK, Rathi R, Chen F, Honaramooz A, Dobrinski I. Preservation and transplantation of porcine testis tissue. Reproduction, Fertility and Development. 2009;21：489-497. (IF= 2.44)

Tang L*, Zeng W*, Clark RK, Dobrinski I. Characterization of the porcine testis-expressed gene 11 (Tex11). Spermatogenesis. 2011；1：147-51 (第一，二作者貢獻相同)

Dong W, Zhang X, Yang C, Qin J, Song F, Zeng W*. Iridovirus Outbreak in Chinese Giant Salamanders, China, 2010. Emerging Infectious Deseases, 2011, 17(12):2388-2389. (IF= 6.79)

He Z, Kokkinaki M, Jiang J, Zeng W, Dobrinski I, Dym M. Isolation of human male germ-line stem cells using enzymatic digestion and magnetic-activated cell sorting. Methods Molecular Biology. 2012; 825:45-57.

Arregui L, Rathi R, Modelski M, Zeng W, Roldan ER, Dobrinski I. Suppression of spermatogenesis before grafting increases survival and supports resurgence of spermatogenesis in adult mouse testis. Fertility and Sterility. 2012;97(6):1422-9. (IF= 3.78)

Behboodi E, Bondareva A, Begin I, Rao K, Neveu N, Pierson JT, Wylie C, Piero FD, Huang YJ, Zeng W, Tanco V, Baldassarre H, Karatzas CN, Dobrinski I, Establishment of goat embryonic stem cells from in vivo produced blastocyst-stage embryos. Molecular Reproduction and Development. 2011;78:202-11. (IF=″2.40)

Li M, Liu Y, Wang T, Guan J, Luo Z, Chen H, Wang X, Chen L, Ma J, Mu Z, Jiang A, Zhu L, Lang Q, Zhou X, Wang J, Zeng W, Li N, Li K, Gao X, Li W. Repertoire of porcine microRNAs in adult ovary and testis by deep sequencing. International Journal of Biological Sciences. 2011; 7:1045-55. (IF= 3.22)

Zhang X, Chou W, Haig-Ladewig L, Zeng W, Cao W, Gerton G, Dobrinski I, Tseng H. BNC1 is required for maintaining mouse spermatogenesis. Genetics. 2012；50：517-24. (IF= 4.01)

Tolkunova E, Malashichevaa A, Chikhirjina ЕV, Kostyleva EI, Zeng W, Luo J, Dobrinski I, Hierholzer А, Kemler R, Tomilin А. E-Cadherin as a Novel Surface Marker of Spermatogonial Stem cells. Cell and Tissue Biology. 2009; 3:103-9.

Rathi R*, Zeng W*, Megee S, Conley A, Meyers S, Dobrinski I. Maturation of testicular tissue from infant monkeys after xenografting into mice. Endocrinology. 2008; 149(10):5288-96. (第一，二作者貢獻相同) (IF= 5.05)

Honaramooz A, Megee S, Zeng W, Destrempes M, Overton S, Luo J, Galantino-Homer H, Modelski M, Chen F, Blash S, Melican D, Gavin W, Ayres S, Yang F, Wang J, Echelard Y, Dobrinski I. Adeno-associated virus (AAV)-mediated transduction of male germline stem cells results in transgene transmission after germ cell transplantation. The FASEB Journal (The Federation of American Societies for Experimental Biology Journal). 2008; 22(2):374-82. (IF= 6.79)

Bailey J, Lessard C, Jacques J, Brèque C, Dobrinski I, Zeng W, Galantino-Homer H. Cryopreservation of boar semen and its future importance to the industry. Theriogenology 2008; 70:1251-9. (IF= 1.91)

Arregui L, Rathi R, Zeng W, Honaramooz A, Gomendio M, Roldan ERS, Dobrinski I. Xenografting of adult mammalian testis tissue. Animal Reproduction Science. 2008;106(1-2):65-76. (IF= 1.74)

Zeng W, Rathi R, Pan H, Dobrinski I. Comparison of global gene expression between porcine testis tissue xenografts and porcine testis in situ. Molecular Reproduction and Development. 2007; 74(6): 674-9. (IF= 2.38)

Zeng W, Avelar GF, Rathi R, Franca LR, Dobrinski I. The length of the spermatogenic cycle is conserved in porcine and ovine testis xenografts. Journal of Andrology. 2006; 27(4):527-33. (IF= 2.04)

Turner RM, Rathi R, Zeng W, Honaramooz A, Dobrinski I. Xenografting to study testis function in stallions. Animal Reproductive Science. 2006;94:161-4. (IF= 2.14)

Rathi R, Honaramooz A, Zeng W, Turner R, Dobrinski I. Germ cell development in equine testis tissue xenografted into mice. Reproduction. 2006;131(6):1091-8. (IF= 3.14)

Galantino-Homer HL, Zeng W, Megee SO, Dallmeyer M, Voelkl D. Dobrinski I. Effects of 2-hydroxypropyl-beta-cyclodextrin and cholesterol on porcine sperm viability and capacitation status following cold shock or incubation. Molecular Reproduction and Development. 2006; 73(5):638-50. (IF= 2.22)

Rathi R, Honaramooz A, Zeng W, Schlatt S, Dobrinski I. Germ cell fate and seminiferous tubule development in bovine testis xenografts. Reproduction. 2005;130(6):923-9. (IF= 3.14 )

Turner RM, Rathi R, Zeng W, Dobrinski I. Xenografting of degenerate stallion testis onto a mouse host does not rescue the testicular degeneration phenotype. Animal Reproductive Science. 2005;89(1-4):253-5. (IF= 2.14)

Zeng W, Terada T. Effects of methyl-beta-cyclodextrin on cryosurvival of boar spermatozoa. Journal of Andrology 2001;22(1):111-118.

Zeng W, Terada T. Protection of boar spermatozoa from cold shock damage by 2-hydroxypropyl-beta-cyclodextrin. Theriogenology 2001;55(2):615-27.

Zeng W, Shimada M, Terada T. Survival of boar spermatozoa frozen in diluents of varying osmolality. Theriogenology 2001;56(3):447-58.

Zeng W, Terada T. Freezability of boar spermatozoa is improved by exposure to 2-hydroxypropyl-beta-cyclodextrin. Reproduction, Fertility and Development. 2000;12(3-4):223-8.

Zeng W, Terada T. Effects of exposure to bovine follicular fluid before freezing on cryoresistance of boar spermatozoa. Animal Science Journal. 2001;72:291-8.

Shimada M, Zeng W, Terada T. Inhibition of PI-3 kinase or MEK leads to suppression of p34 cdc2 kinase activity and meiotic progression beyond the MI stage in porcine oocyte surrounded with cumulus cells. Biology of Reproduction. 2001;65:442-8.

Anas M-K I, Shimada M, Zeng W, Terada T. Meiotic maturation of bovine oocytes cultured in vitro with EGF and wortmannin. Journal of Mammal Ova Research. 2000;17(3):103-8.

Anas M-K I, Shimada M, Zeng W, Terada T. Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, blocks in vitro fertilization and embryonic development of bovine oocytes matured in vitro with epidermal growth factor. Journal of Mammal Ova Research. 2000;17(3):109-14.

Shojo A, Shimada M, Zeng W, Terada T. Activation by stimuli of sperm penetration in bovine oocytes treated with higher concentration of BAPTA/AM. Journal of Mammal Ova Research. 2000;17(3):96-102.

曾文先

基本介紹

人物經歷

研究領域

學術兼職

科研課題

主要論文

主要論文

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