戚正武

戚正武

戚正武，男，1932年4月生於浙江寧波，蘇聯醫科院莫斯科醫學與生物化學研究所博士，同濟大學蛋白質所所長，博士生導師，生物化學家，中科院院士，研究員，《生物化學與生物物理學報》以及國際《血栓研究》雜誌的編委。

基本介紹

  • 中文名：戚正武
  • 畢業院校：蘇聯醫科院莫斯科醫學與生物化學研究所
  • 學位/學歷：博士
  • 專業方向：酶蛋白結構與功能、活性多肽、蛋白質工程
  • 職務：同濟大學蛋白質所所長
人物經歷,研究方向,學術研究,承擔項目,代表論文,

人物經歷

戚正武院士，生物化學家。 1932年出生於浙江寧波。1952年畢業於上海同濟大學化學系。1959年獲蘇聯醫科院莫斯科醫學與生物化學研究所副博士。中國科學院上海生物化學研究所研究員。1978-1984年任生化所蛋白室副主任, 84年後室的編製取消, 為歷屆生化所學術委員會及學位評定委員會成員, 83年被批准為博士生導師, 曾為毛主席遺體保護科研組成員(組長吳階平)及國家生物技術(863計畫)首屆蛋白質工程專題專家組成員。擔任國際期刊Thrombosis Research (1988-1992; 1996-2000)，J. Natural Toxin (1992-1996)及國內期刊生物化學與生物物理學報(1988-1996)，中國生理科學 (英文版)(1985-1988)及動物學研究(1993－)等編委。1999年被評為中國科學院院士。

研究方向

戚正武院士長期以來從事酶蛋白結構與功能的研究，七十年代後期又擴展到活性多肽的研究領域，近年來又開展蛋白質工程研究。蛋白酶抑制劑、蠍神經毒蛋白、芋螺毒素與凝血纖溶有關的蛋白酶及其抑制劑的研究居國際前沿。

學術研究

長期以來從事活性多肽及酶蛋白結構與功能的研究，利用蛋白質工程技術研究結構與功能的關係。有關蛋白酶抑制劑，蠍及芋螺多肽神經毒素以及與凝血纖溶有關的蛋白質的研究居國際前沿。曾從不同植物中分離純化了三種不同類型的蛋白酶抑制劑，闡明了它們的蛋白質、基因及晶體結構，確定了活性中心位置，其中多功能雙頭慈菇蛋白酶抑制劑是屬於新的抑制劑家族。利用多肽合成，蛋白酶限止性降解及蛋白質工程等手段研究了它們的結構與功能的關係。在基因水平上闡明了異常人血清白蛋白及血小板無力症遺傳性疾病的基因缺陷。對凝血因子Ⅷ及vWF因子進行了基因克隆，並在哺乳動物細胞中獲得表達，申請了生產重組凝血因子Ⅷ的專利。90年代以來又開展研究蠍及芋螺毒腺中各種離子通道及神經遞質受體的神經毒素,發現了多種結構全新的神經毒素。此外在豬肝中分離純化了與體內重要生理功能相關的天然蛋白質前體加工酶的抑制劑。
曾發表論文百餘篇, 大多數刊登在國外主要學術期刊及英文版“中國科學”上。曾獲得中國科學院自然科學獎二等獎3次，一等獎1次, 國家自然科學獎三等獎2次及其它省部級科技獎。1981年慕尼黑國際激肽會議上獲優秀論文獎。 至今已培 養博士生及碩士生各20名。

承擔項目

1. 973項目：新活性多肽最佳化設計及與靶分子相互作用的理論模擬；
2. 國家自然科學基金與廣東聯合基金項目：抗登革熱病毒NS3蛋白酶小分子化合物和多肽抑制劑的合理設計研究。

代表論文

  1. Yuan DD, Liu L, Shao XX, Peng C, Chi CW, Guo ZY. Isolation and cloning of a conotoxin with a novel cysteine pattern from Conus caracteristicus. Peptides. 2008 Sep;29(9):1521-5. Epub 2008 May 25.
  2. Wang Q, Jiang H, Han YH, Yuan DD, Chi CW. Two different groups of signal sequence in M-superfamily conotoxins. Toxicon. 2008 Apr;51(5):813-22. Epub 2007 Dec 15.
  3. Qian J, Guo ZY, Chi CW. Cloning and isolation of a Conus cysteine-rich protein homologous to Tex31 but without proteolytic activity. Acta Biochim Biophys Sin (Shanghai). 2008 Feb;40(2):174-81.
  4. Rui-feng Qi, Ling Zhang, and Cheng-wu Chi. Biological characteristics of dengue virus and potential targets for drug design. Acta Biochim Biophys Sin. 2008 Feb;40(2):91-101.
  5. Wang ZQ, Han YH, Shao XX, Chi CW, Guo ZY. Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus. FEBS J. 2007 Sep;274(18):4778-87. Epub 2007 Aug 14.
  6. Yuan DD, Han YH, Wang CG, Chi CW. From the identification of gene organization of alpha conotoxins to the cloning of novel toxins. Toxicon. 2007 Jun 15;49(8):1135-49. Epub 2007 Mar 1.
  7. Du WH, Han YH, Huang FJ, Li J, Chi CW, Fang WH. Solution structure of an M-1 conotoxin with a novel disulfide linkage. FEBS J. 2007 May;274(10):2596-602. Epub 2007 Apr 16.
  8. Han YH, Wang Q, Jiang H, Liu L, Xiao C, Yuan DD, Shao XX, Dai QY, Cheng JS, Chi CW. Characterization of novel M-superfamily conotoxins with new disulfide linkage. FEBS J. 2006 Nov;273(21):4972-82. Epub 2006 Oct 17.
  9. Chen-Qi Xu, Shun-Yi Zhu, Cheng-Wu Chi and Jan Tytgat. Turret and pore block of K+Channels: what is the different? Trends Pharmac. Sci. 24(9): 446-449, 2003.
  10. Fan CX, Chen XK, Zhang C, Wang LX, Duan KL, He LL, Cao Y, Liu SY, Zhong MN, Ulens C, Tytgat J, Chen JS, Chi CW, and Zhou Z. A novel conotoxin from conus betulinus, k-BtX, unique in cysteine pattern and in function as a specific BK channel modulator. J Biol Chem. 278(15), 12624-12633, 2003.
  11. Wang CG, Gilles N, Hamon A, Le Gall F, Stankiewicz M, Pelhate M, Xiong YM, Wang DC and Chi CW. Exploration of the functional site of a scorpion alpha-like toxin by site-directed mutagenesis. Biochemistry. 42 (16), 4699-4708, 2003.
  12. Wang CG, Stankiewicz M, Ling MH, Pelhate M, Wang DC and Chi CW*. Purification of two depressant insect neurotoxins and their gene cloning from the scorpion Buthus martensi Karsch. J. Pept. Res. 61(1): 7-16, 2003.
  13. C. Goudet, Cheng-wu Chi, J Tytgat. An overview of toxins and genes from the venom of the Asian scorpion Buthus martensi Karsch. Toxicon. 40(9), 1239, 2002.
  14. Jiong Li, Kang-cheng Ruan, Cheng-wu Chi. The assignment of the reactive sites of the double-headed arrowhead proteinase inhibitor A and B. Acta Biochi. Biophys Sinica. 34, 662, 2002.
  15. Jiong Li, Cheng-wu Chi, Kang-cheng Ruan. Conformation nearby Trp residues of APIA and APIB modulates the inhibitory specificity of the protease. Acta Biochi. Biophys Sinica. 34, 494, 2002.
  16. Jing-Jiang Wu, Lin-Lin He, Zhuan Zhou and Cheng-Wu Chi. Gene expression, mutation and structure-function relationship of scorpion toxin BmP05 active on SKCa channels. Biochemistry. 41, 2844, 2002
  17. Chun Chen, Qi Wang, Xiang-dong Fang, Qin Xu, Cheng-wu Chi and Jian-xin Gu. Roles of the phytanoyl CoA ?-hydroxylase in mediating the expression of human coagulation factor VIII. J. Biol. Chem. 276, 46340, 2001.
  18. Guang-yu Zhu, Qi-chen Huang, Yan-shi Zhu, Cheng-wu Chi, You-qi Tang. X-ray study on an artificial mung bean inhibitor complex with bovine ?-trypsin in neat cyclohexane. Biochim. Biophys. Acta 1546, 98, 2001.
  19. Chun-Guang Wang, Xiao-Lin He, Feng Shao, Wei Liu, Min-Hua Ling, Da-Cheng Wang, Cheng-Wu Chi. Molecular characterization of an anti-epilepsy peptide from the scorpio Buthus martensi Karsch. Eur. J. Biochem. 268, 2480, 2001.
  20. Xiang-Dong Fang, Chun Chen, Qi Wang, Jian-Xin Gu, Cheng-Wu Chi. The Interaction of the Calcium and Integrin Binding Protein (CIBP) with the Coagulation Factor VIII. Thromb. Res. 102, 177, 2001.
  21. Hao Fei, Yan LI, Li-Xiu Wang, Ming-Juan Luo, Min-Hua Ling, Cheng-Wu Chi. Purified nonhistone protein from porcine kidney acts as a suicide substrate inhibitor on furin-like enzyme. Acta Pharmacol. Sin. 21, 265, 2000.
  22. Li Dai, Jing-jiang Wu, Yi-hua Gu, Zheng-dao Lan, Min-hua Ling and Cheng-wu Chi. Genomic organization of three novel toxins from the scorpion Buthus martinsi Karsch that are active on potassium channels. Biochem. J. 346, 805, 2000.
  23. Jing-jiang Wu, Li Dai, Zheng-dao Lan and Cheng-wu Chi. The Gene Cloning and Sequencing of Bm-12, a Chlorotoxin-like Peptide from the Scorpion Buthus martinsi Karsch. Toxicon 38, 661, 2000.
  24. Feng Shao, Yu-mei Xiong, Rong-huan Zhu, Min-hua Ling, Cheng-wu Chi & Da-cheng Wang. Expression and Purification of the BmK M1 Neurotoxin from the Buthus martinsi Karsch. Protein Expression and Purification 17, 358, 1999.
  25. Yan-shi Zhu, Qi-chen Huang and Cheng-wu Chi. Crystal structure of mung bean inhibitor lysine active fragment complex with bovine beta-trypsin at 1.8A resolution. J Biomol Struct Dyn. 1999 Jun;16(6):1219-24.
  26. Chun Chen, Xiao-dong Fang, Jiang Zhu, Xiang-fu Wu, Zu-chuan Zhang, Zheng-yi Wang and Cheng-wu Chi. The Gene Expression of Coagulation Factor VIII in Mammalian Cell Lines. Thrombosis Res. 95, 105, 1999.
  27. Jing-jiang Wu, Li Dai, Zheng-dao Lan and Cheng-wu Chi. Genomic Organization of Three Neurotoxins Active on Small Conductance Ca++-activated Potassium Channels from the Scorpion Buthus martinsi Karsch. FEBS Letters 452, 360, 1999.
  28. Yu-mei Xiong, Zheng-dao Lan, Miao Wang. Bo Liu, Xin-qi Liu, Hao Fei, Lai-gen Xu, Qi-Chang Xia, Chung-guang Wang, Da-cheng Wang and Cheng-wu Chi. Molecular Characterization of a New Excitatory Insect Neurotoxin with an Analgesic Effect on Mice from the Scorpion Buthus martinsi Karsch. Toxicon 37, 1165, 1999.
  29. Zheng-dao Lan, Li Dai, Xiao-liang Zhuo, Ke Xu, Jun-Cai Feng and Cheng-wu Chi. Gene Cloning and Sequencing of BmK AS and BmK As-1, Two Novel Neurotoxins from the Scorpion Buthus martinsi Karsch. Toxicon. 37, 815, 1999.
  30. Yu-mei Xiong, Min-hua Ling, Zheng-dao Lan, Da-cheng Wang and Cheng-wu Chi .The cDNA Sequence of an Excitatory Insect Selective Neurotoxin from the Scorpion Buthus martinsi Karsch. Toxicon 37, 335, 1999.
  31. Yu-mei Xiong, Min-hua Ling, Da-cheng Wang and Cheng-wu Chi. The cDNA and Genomic DNA Sequences of a Mammalian Neurotoxin from the Scorpion Buthus martensii Karsch. Toxicon 35, 1025, 1997.
  32. Ming-juan Luo, Yu-mei Xiong, Miao Wang, Da-cheng Wang and Cheng-wu Chi. Purification and Sequence Determination of a New Neutral Mammalian Neurotoxin from Scorpion Buthus martensii Karsch. Toxicon 35, 723, 1997.
  33. Ming-juan Luo, Wu-yuan Lu and Cheng-wu Chi. Clarification of an Uncertain Intron within the cDNA Sequences of Arrowhead Proteinase Inhibitor A and B. J. Biochem. (Tokyo) 121, 991, 1997.
  34. Zhi-wei Xie, Ming-juan Luo, Wen-feng Xu and Cheng-wu Chi. Two Reactive Site Location and Structure-Function Study of the Arrowhead Protease Inhibitors,A and B, Using Mutagenesis. Biochemistry (US) 36, 5846, 1997.

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