《microRNA在果梅雌蕊敗育中的調控作用》是高志紅為項目負責人，南京農業大學為依託單位的青年科學基金項目。
基本介紹
- 中文名：microRNA在果梅雌蕊敗育中的調控作用
- 項目類別 ：青年科學基金項目
- 依託單位 ：南京農業大學
- 項目負責人：高志紅
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | 中國原產果梅自交親和變異品種花粉決定基因SFB的插入突變 | 期刊論文 | 王培培|侍婷|高志紅|章鎮|莊維兵| |
2 | High-Throughput Sequencing of small RNAs and Analysis of Differentially Expressed microRNAs Associated with Pistil Development in Japanese apricot | 期刊論文 | Zhihong Gao|Ting Shi|Xiaoyan Luo|Zhen Zhang|Weibing Zhuang|Liangjju Wang| |
3 | 落葉果樹需冷量及其估算模型研究進展 | 期刊論文 | 莊維兵|章鎮|侍婷|王培培|邵靜|羅曉燕|高志紅| |
4 | Sequence Analysis of New S-RNase and SFB alleles in Japanese Apricot (Prunus mume) | 期刊論文 | Gao, ZhiHong|Wang, PeiPei|Zhuang, WeiBing|Zhang, Zhen| |
5 | Self-compatibility in ‘Zaohong’ Japanese apricot is associated with the loss of function of pollen S genes | 期刊論文 | Pei-Pei Wang|Zhi-Hong Gao|Zhao-Jun Ni|Zhen Zhang|Binhua Cai| |
6 | Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume) | 期刊論文 | P.P. Wang*, Z.H. Gao*, Z.J. Ni, W.B. Zhuang and Z.| |
7 | Determination of S-RNase genotypes and isolation of four novel S-RNase genes in Japanese apricot (Prunus mume Sieb. et Zucc.) native to China | 期刊論文 | Wang, P. P.|Shi, T.|Zhuang, W. B.|Zhang, Z.|Gao, Z. H.| |
8 | Comparative proteomic analysis of pistil abortion in Japanese apricot (Prunus mume Sieb. et Zucc) | 期刊論文 | Ting Shi|Zhihong Gao|Weibing Zhuang|Zhen Zhang|Hailong Sun|Liangju Wang| |
9 | Identification and Validation of Potential Conserved microRNAs and Their Targets in Peach (Prunus persica) | 期刊論文 | Zhihong Gao|Xiaoyan Luo|Ting Shi|Binhua Cai|Zhen Zhang|Weibing Zhuang| |
10 | KNOX I類基因在雌蕊發育中的作用 | 期刊論文 | 孫海龍，侍 婷，章 鎮，高志紅| |
11 | Comparative proteomic and transcriptomic approaches to<br />address the active role of GA4 in Japanese apricot flower bud<br />dormancy release | 期刊論文 | Weibing Zhuang|Zhihong Gao|Liangju Wang|Wenjun Zhong|Zhaojun Ni|Zhen Zhang| |
12 | 果梅自交不親和相關基因型(S、SFB/SLF)研究進展 | 期刊論文 | 王培培|高志紅|章鎮| |
13 | Differential expression of proteins associated with seasonal bud dormancy at four critical stages in Japanese apricot Plant Biology | 期刊論文 | Weibing Zhuang|Ting Shi|Zhihong Gao|Zhen Zhang| |
14 | Identification of Differentially-Expressed Genes Associated with Pistil Abortion in Japanese Apricot by Genome-Wide Transcriptional Analysis | 期刊論文 | Shi, Ting|Gao, Zhihong|Wang, Liangju|Zhang, Zhen|Zhuang, Weibing|Sun, Hailong|Zhong, Wenjun| |
15 | Evaluation of Chilling and Heat Requirements in Japanese Apricot with Three Models | 期刊論文 | Gao, Zhihong|Zhuang, Weibing|Wang, Liangju|Shao, Jing|Luo, Xiaoyan|Cai, Binhua|Zhang, Zhen| |
16 | Genome-wide expression profiles of<br />seasonal bud dormancy at four critical<br />stages in Japanese apricot | 期刊論文 | Wenjun Zhong|Zhihong Gao|Weibing Zhuang|Ting Shi|Zhen Zhang|Zhaojun Ni| |
17 | Differential expression of proteins associated with seasonal bud dormancy at four critical stages in Japanese apricot | 期刊論文 | Zhuang, W. -B.|Shi, T.|Gao, Z. -H.|Zhang, Z.|Zhang, J. -Y.| |
18 | Identification of miRNAs and Their Target Genes in Peach (Prunus persica L.) Using High-Throughput Sequencing and Degradome Analysis | 期刊論文 | Xiaoyan Luo|Zhihong Gao*|Ting Shi|Zongming Cheng|Zhen Zhang|Zhaojun Ni| |
項目摘要
本研究結合項目組多年果梅種質資源和分子生物學的研究基礎，在採用石蠟切片找出雌蕊敗育關鍵時期的基礎上，擬通過高通量測序分離與果梅雌蕊敗育相關的microRNAs，預測其靶基因並通過螢光定量PCR、RLM-RACE以及轉基因進行表達分析和功能鑑定，初步篩選出與雌蕊敗育相關的關鍵microRNAs，然後利用原位雜交技術等對上述關鍵microRNAs進行細胞定位及其對AGAMOUS等雌蕊調控基因表達的影響，鑑定出果梅雌蕊敗育的關鍵microRNAs，初步明確microRNA在果梅雌蕊選擇性敗育中作用，為揭示果梅雌蕊敗育的分子機制提供依據。
