《Cdc42-LIMK-Cofilin信號通路對卵母細胞極性的調控》是孫少琛為項目負責人，南京農業大學為依託單位的面上項目。
基本介紹
- 中文名：Cdc42-LIMK-Cofilin信號通路對卵母細胞極性的調控
- 項目類別 ：面上項目
- 項目負責人：孫少琛
- 依託單位 ：南京農業大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Protective Effects of Melatonin Against Zearalenone Toxicity on Porcine Embryos in vitro | 期刊論文 | Xu Yao(#); Zhang Kun Huan; Sun Ming Hong; Lan Mei; Wan Xiang; Zhang Yu; Sun Shao Chen(*) |
2 | Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis | 期刊論文 | Lu Yujie(#); Zhang Yue; Liu Jia Qian; Zou Peng; Jia Lu; Su Yong Teng; Sun Yu Rong; Sun Shao Chen(*) |
3 | Daam1 regulates fascin for actin assembly in mouse oocyte meiosis | 期刊論文 | Lu Yujie(#); Zhang Yu; Pan Meng Hao; Kim Nam Hyung; Sun Shao Chen(*); Cui Xiang Shun(*) |
4 | Inhibition of Rac1 GTPase activity affects porcine oocyte maturation and early embryo development | 期刊論文 | Song Si Jing(#); Wang Qiao Chu; Jia Ru Xia; Cui Xiang Shun; Kim Nam Hyung; Sun Shao Chen(*) |
5 | HT-2 toxin exposure induces mitochondria dysfunction and DNA damage during mouse early embryo development | 期刊論文 | Zhang Liping(#); Li Lishu; Xu Jie; Pan Meng Hao; Sun Shao Chen(*) |
6 | Involvement of LIMK1/2 in actin assembly during mouse embryo development | 期刊論文 | Duan Xing(#); Zhang Hao-Lin; Wu Lan-Lan; Liu Meng-Yao; Pan Meng-Hao; Ou Xiang-Hong(*); Sun Shao Chen(*) |
7 | Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics | 期刊論文 | Wang Hong Hui(#); Zhang Yu; Tang Feng; Pan Meng Hao; Wan Xiang; Li Xiao Han; Sun Shao Chen(*) |
8 | Profilin 1 plays feedback role in actin-mediated polar body extrusion in mouse oocytes | 期刊論文 | Liu Jun(#); Wang Qiao Chu; Duan Xing; Cui Xiang Shun; Kim Nam Hyung; Zhang Yu; Sun Shao Chen(*) |
9 | Actin cytoskeleton dynamics in mammalian oocyte meiosis | 期刊論文 | Duan, Xing(#); Sun, Shao Chen(*) |
10 | Involvement of Kif4a in Spindle Formation and Chromosome Segregation in Mouse Oocytes | 期刊論文 | Tang Feng(#); Pan Meng Hao; Lu Yujie; Wan Xiang; Zhang Yu; Sun Shao Chen(*) |
11 | Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes | 期刊論文 | Wan Xiang(#); Zhang Yu; Lan Mei; Pan Meng-Hao; Tang Feng; Zhang Hao-Lin; Ou Xiang-Hong(*); Sun Shao Chen(*) |
12 | 霍英東青年教師獎 | 科研獎勵 | 孫少琛 |
13 | Effects of obesity and diabetes on the epigenetic modification of mammalian gametes | 期刊論文 | Ou, Xiang Hong(#); Zhu, Cheng Cheng; Sun, Shao Chen(*) |
14 | Melatonin ameliorates ochratoxin A-induced oxidative stress and apoptosis in porcine oocytes | 期刊論文 | Lan Mei(#); Zhang Yu; Wan Xiang; Pan Meng-Hao; Xu Yao; Sun Shao Chen(*) |
15 | Vesicular transport protein Arf6 modulates cytoskeleton dynamics for polar body extrusion in mouse oocyte meiosis | 期刊論文 | Duan Xing(#); Zhang Hao Lin; Pan Meng Hao; Zhang Yu; Sun Shao Chen(*) |
16 | Melatonin protects oocytes from MEHP exposure-induced meiosis defects in porcine | 期刊論文 | Zhang Yu(#); Wang Teng; Lan Mei; Zang Xin Wei; Li Yi Lun; Cui Xiang Shun; Kim Nam Hyung; Sun Shao Chen(*) |
17 | HT-2 toxin affects development of porcine parthenotes by altering DNA and histone methylation in oocytes matured in vitro | 期刊論文 | Zhang Yue(#); Jia Ru Xia; Pan Meng Hao; Lu Yujie; Cui Xiang Shun; Kim Nam Hyung; Sun Shao Chen(*) |
18 | FHOD1 regulates cytoplasmic actin-based spindle migration for mouse oocyte asymmetric cell division | 期刊論文 | Pan Meng-Hao(#); Wang Fei; Lu Yujie; Tang Feng; Duan Xing; Zhang Yu; Xiong Bo; Sun Shao Chen(*) |
19 | Loss of PKC mu function induces cytoskeletal defects in mouse oocyte meiosis | 期刊論文 | Zhang, Yu(#); Wu, Lan Lan; Wan, Xiang; Wang, Hong Hui; Li, Xiao Han; Pan, Zhen Nan; Sun, Shao Chen(*) |
20 | Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis | 期刊論文 | Tang Feng(#); Pan Meng-Hao; Wan Xiang; Lu Yujie; Zhang Yu; Sun Shao Chen(*) |
21 | RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis | 期刊論文 | Zhang, Yu(#); Wan, Xiang; Wang, Hong Hui; Pan, Meng Hao; Pan, Zhen Nan; Sun, Shao Chen(*) |
22 | The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis | 期刊論文 | Duan Xing(#); Zhang Yu; Chen Kun Lin; Zhang Hao Lin; Wu Lan Lan; Liu Hong Lin; Wang Zhen Bo; Sun Shao Chen(*) |
23 | 教育部高等學校科學研究優秀成果獎(科學技術) | 科研獎勵 | 劉紅林; 孫少琛; 李齊發; 申明; 張羽 |
24 | Altered apoptosis/autophagy and epigenetic modifications cause the impaired postimplantation octaploid embryonic development in mice | 期刊論文 | Wu Bao Jiang(#); Zhao Li Xia; Zhu Cheng Cheng; Chen Yang Lin; Wei Meng Yi; Bao Si Qin; Sun Shao Chen(*); Li Xi He(*) |
25 | The small GTPase CDC42 regulates actin dynamics during porcine oocyte maturation | 期刊論文 | Zhang Yu(#); Wang Qiao Chu; Liu Jun; Xiong Bo; Cui Xiang Shun; Kim Nam Hyung; Sun Shao Chen(*) |
26 | LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation | 期刊論文 | Jia Ru Xia(#); Duan Xing; Song Si Jing; Sun Shao Chen(*) |
27 | RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis | 期刊論文 | Pan, Zhen Nan(#); Lu, Yujie; Tang, Feng; Pan, Meng Hao; Wan, Xiang; Lan, Mei; Zhang, Yu; Sun, Shao Chen(*) |
28 | Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation | 期刊論文 | Zhang Yu(#); Wang Hong-Hui; Wan Xiang; Xu Yao; Pan Meng-Hao; Sun Shao Chen(*) |
29 | Melatonin protects against defects induced by deoxynivalenol during mouse oocyte maturation | 期刊論文 | Lan Mei(#); Han Jun; Pan Meng Hao; Wan Xiang; Pan Zhen Nan; Sun Shao Chen(*) |
項目摘要
不同於體細胞，哺乳動物的卵母細胞具有極性，導致其獨特的不均等減數分裂。這種不均等分裂對於卵胞質內的營養和遺傳物質的保存是至關重要的，直接影響到後期的受精以及胚胎髮育。到現在為止，對於卵母細胞不均等分裂的分子機制了解的還很少。之前的工作證明微絲直接參與了卵母細胞的極性形成。而我們前期的研究證明微絲相關蛋白Arp2/3複合物參與了這一過程，但是，還不清楚G蛋白酶相關效應因子是否參與了哺乳動物卵子的不均等分裂。為了研究G蛋白酶相關因子在卵母細胞極性形成過程中的功能和機制，本項目將採用RNA干擾、過表達、基因敲除等技術手段，結合活細胞工作站觀察，深入研究G蛋白酶Cdc42效應因子LIMK和Cofilin在小鼠卵子紡錘體遷移和定位，極性產生、極體排出等過程中的功能，並闡明其作用機制和信號通路，進一步拓展對卵母細胞極性產生的認識，為卵子質量控制、受精及後期胚胎髮育的調控提供理論基礎。
