鵝Toll樣受體分子克隆及其介導的宿主抗NGVEV感染機制研究

科研成果

序號	標題	類型	作者
1	鴨傳染性漿膜炎滅活疫苗	科研獎勵	-
2	鴨傳染性漿膜炎滅活疫苗	科研獎勵	-
3	Chinese goose (Anser cygnoides) CD8a: Cloning, tissue distribution and immunobiological in splenic mononuclear cells	期刊論文	Zhu, Dekang\|Liu, Mafeng\|Chen, Xiaoyue\|Cheng, Anchun\|
4	Type I interferon receptors in goose: Molecular cloning, structuralidentification, evolutionary analysis and age-related tissueexpression profile	期刊論文	陳舜\|
5	Development and validation of an efficient real-time quantitative reversetranscription polymerase chain reaction assay of Chinese Goose CD4 and CD8α	期刊論文	Renyong Jia\|Dekang Zhu\|Mafeng Liu\|Anchun Cheng\|
6	Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection	期刊論文	陳舜\|
7	Molecular cloning, tissue distribution and immunology function of Goose TLR7	期刊論文	Mafeng Liu\|Fei Liu\|Xiaoyue Chen\|Anchun Cheng\|
8	Interferons and their receptors in birds: a comparison of gene structure, phylogenetic analysis, and cross modulation	期刊論文	Hao Zhou\|Shun Chen\|Mingshu Wang\|Anchun Cheng\|
9	Identification, Characterization, and DevelopmentalExpression Pattern of Type III Interferon Receptor Gene inthe Chinese Goose	期刊論文	陳舜\|
10	Evolutionary characterization of Tembusu virus infection through identification of codon usage patterns	期刊論文	Shun Chen\|Renyong Jia\|Mingshu Wang\|Anchun Cheng\|
11	Molecular cloning, characterization and tissue expression of CD4 in Chinese goose	期刊論文	陳舜\|
12	innate sensing of viruses by pattern recognition receptors in birds	期刊論文	Shun Chen\|Anchun Cheng\|Mingshu Wang\|
13	細小病毒非結構蛋白誘導細胞凋亡研究進展	期刊論文	陳舜\|劉菲\|汪銘書\|程安春\|
14	Immunobiological activity and antiviral regulation efforts of chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection	期刊論文	Dekang Zhu\|Mafeng Liu\|Xiaoyue Chen\|Anchun Cheng\|
15	Molecular cloning, tissue distribution, and immune function of gooseTLR7	期刊論文	陳舜\|
16	chinese goose (anser cygnoides) CD8a: cloning, tissue distribution and immunobiological in splenic mononuclear cells	期刊論文	Dekang Zhu\|Mafeng Liu\|Xiaoyue Chen\|Anchun Cheng\|
17	Development and validation of a SYBR Green real-time PCR assay for rapid and quantitative detection of goose interferons and proinflammatory cytokines	期刊論文	陳舜\|
18	Age-related development and tissue distribution of T cell markers(CD4 and CD8a) in Chinese goose	期刊論文	陳舜\|
19	禽類抗病毒相關TOLL 樣受體研究進展	期刊論文	閆冰\|陳舜\|汪銘書\|程安春\|
20	Innate sensing of viruses by pattern recognition receptors in birds	期刊論文	Shun Chen\|Anchun Cheng\|Mingshu Wang\|
21	禽類干擾素受體介導的抗病毒免疫機制研究進展	期刊論文	周浩\|陳舜\|汪銘書\|程安春\|
22	Identification of Type II Interferon Receptors in Geese: Gene Structure, Phylogenetic Analysis, and Expression Patterns	期刊論文	陳舜\|
23	Role of capsid proteins in parvoviruses infection	期刊論文	陳舜\|
24	Molecular cloning, characterization and tissue expression of CD4 in Chinese goose	期刊論文	Jia, Renyong\|Zhu, Dekang\|Chen, Xiaoyue\|Cheng, Anchun\|
25	CpG oligodeoxynucleotide-specific goose TLR21 initiates an anti-viralimmune response against NGVEV but not AIV strain H9N2 infection	期刊論文	陳舜\|
26	四川白鵝CD4 基因胞外去信號肽區的可溶性表達及純化	期刊論文	程蓓蓓\|陳舜\|汪銘書\|程安春\|
27	Transcriptome Analysis and Identification of Differentially Expressed Transcripts of Immune-Related Genes in Spleen ofGosling and Adult Goose	期刊論文	陳舜\|

項目摘要

哺乳動物Toll樣受體(Toll like receptors,TLR)在宿主先天抗感染免疫中發揮重要作用，而鵝TLRs的分子特徵和抗病毒感染的免疫功能仍不清楚。本項目利用RT-PCR和RACE技術從鵝組織中克隆出TLRs的cDNA全序列，經生物信息學分析明確其分子結構並構建分子遺傳進化樹。利用基因表達定量和免疫組織化學染色，明確TLRs在健康鵝體及NGVEV感染鵝體內的細胞定位、組織表達譜及其因感染而發生變化的規律。進一步以原代鵝免疫細胞為模型，利用基因表達定量鑑定鵝TLRs的激動劑，並明確下游細胞因子的表達情況。在此基礎上，通過共轉染報告基因和目標基因、SiRNA基因沉默技術及免疫中和技術明確NGVEV與TLRs的識別、結合及其相互作用的分子機制。本研究將闡明鵝TLRs的生物學特性並明確其介導的宿主抵抗NGVEV感染的分子機制，研究結果將豐富水禽先天抗病毒免疫學基礎知識。

鵝Toll樣受體分子克隆及其介導的宿主抗NGVEV感染機制研究

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科研成果

項目摘要

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