韓建永

韓建永，男，博士，中國農業大學生物化學與分子生物學系教授、博士生導師。

基本介紹

• 中文名：韓建永
• 畢業院校：中國農業大學
• 學位/學歷：博士
• 專業方向：生物化學與分子生物學
• 任職院校：中國農業大學

人物經歷

教育經歷

1993.9-2000.7 河北農業大學，動物科技學院，攻讀本科、碩士學位

2003.9-2006.7 中國農業大學，生物學院，攻讀博士學位

工作經歷

2000.07-2003.09 河北農業大學，動物科技學院，助教、講師

2006.10-2011.03 新加坡基因組研究院，博士後、助理研究員、研究院（Research Scientist）

2011.03-至今 中國農業大學生物學院，教授、博士生導師

研究方向

（1）早期胚胎髮育及其調控機制 利用單細胞技術如單細胞轉錄組檢測等解析小鼠、大家畜（豬、牛等）早期胚胎髮育分化以及多能性調控機制，為高質量多能幹細胞建系和胚胎工程實施提供理論依據。

（2）豬等大動物初始態(naive)胚胎幹細胞建系 分離具有生殖系嵌合能力的豬等大動物胚胎幹細胞，為臨床醫學和畜牧業提供模型細胞和種質細胞。

（3）幹細胞多能性和體細胞重編程調控機制解析 利用基因編輯、體細胞核移植（克隆）顯微操作以及誘導多能幹細胞（iPSC)等技術平台，結合基因組水平測序和分析等分子生物學技術手段和方法研究多能幹細胞質量影響因子、體細胞重編程機理和相關發育生物學問題。

（4）細胞模型和動物模型構建 結合基因編輯和多能幹細胞技術，針對臨床疑難疾病和畜牧業生產性狀，研製細胞模型和動物模型，為攻克人類重要疾病、提高動物生產性狀和抗病能力提供技術平台。

（5）幹細胞新技術研發 在實驗室已有技術平台基礎上，研發適用於科研和生產的新技術，如幹細胞重構胚胎樣結構等。

主講課程

研究生課程：《高級動物生理學》

本科生課程：《動物克隆與轉基因》、《生命科學導論》

學術成果

發表論文

1. Shaopeng Zhang, Tianzhi Chen, Naixin Chen, Dengfeng Gao, Bingbo Shi, Shuangbo Kong, Rachel C. West, Ye Yuan, Minglei Zhi, Qingqing Wei, Jinzhu Xiang, Haiyuan Mu, Liang Yue, Xiaohua Lei, Xuepeng Wang, Liang Zhong, Hui Liang, Suying Cao, Haibin Wang, Juan Carlos Izpisua Belmonte, Jianyong Han*. Implantation initiation of self-assembled embryos generated using three types of mouse blastocyst-derived stem cells. Nature Communications. 2018 Dec (Accepted) (*Corresponding author)

2. Xu J, Yu L, Guo J, Xiang J, Zheng Z, Gao D, Shi B, Hao H, Jiao D, Zhong L, Wang Y, Wu J, Wei H, Han J*. Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system. Stem Cell Res Ther. 2019 Jun 27;10(1):193. doi: 10.1186s13287-019-1303-0. (*Corresponding author)

3. Lineage specification revealed by single-cell gene expression analysis in porcine preimplantation embryos. Wei Q, Li R, Zhong L, Mu H, Zhang S, Yue L, Xiang J, Li E, Zhi M, Cao S, Han J*. Biol Reprod. 2018 Aug 1;99(2):283-292. doi: 10.1093biolreioy062. PMID: 29546319 (*Corresponding author)

4. Xiang J, Cao S, Zhong L, Wang H, Pei Y, Wei Q, Wen B, Mu H, Zhang S, Yue L, Yue G, Lim B, Han J*. Pluripotent stem cells secrete Activin A to improve their epiblast competency after injection into recipient embryos. Protein Cell. 2018 Aug;9(8):717-728. doi: 10.1007s13238-017-0470-y. Epub 2017 Oct 12. (*Corresponding author)

5. Wei Q, Zhong L, Zhang S, Mu H, Xiang J, Yue L, Dai Y, Han J*. Bovine lineage specification revealed by single-cell gene expression analysis from zygote to blastocyst. Biol Reprod. 2017 Jul 1;97(1):5-17. (*Corresponding author)

6. Wang H, Xiang J, Zhang W, Li J, Wei Q, Zhong L, Ouyang H, Han J*. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep. 2016, 6:27256. (*Corresponding author)

7. Wang Y, Li J, Xiang J, Wen B, Mu H, Zhang W, Han J*. Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs. Protein Cell. 2016, 7(2):152-6. (*Corresponding author)

8. Pei Y, Yue L, Zhang W, Wang Y, Wen B, Zhong L, Xiang J, Li J, Zhang S, Wang H, Mu H, Wei Q, Han J*. Improvement in Mouse iPSC Induction by Rab32 Reveals the Importance of Lipid Metabolism during Reprogramming. Sci Rep. 2015, 5:16539. (*Corresponding author)

9. Zhang W, Pei Y, Zhong L, Wen B, Cao S, Han J*. Pluripotent and Metabolic Features of Two Types of Porcine iPSCs Derived from Defined Mouse and Human ES Cell Culture Conditions. PLoS One. 2015 Apr 20;10(4):e0124562. (*Corresponding author)

10. Cao S, Han J*, Wu J, Li Q, Liu S, Zhang W, Pei Y, Ruan X, Liu Z, Wang X, Lim B, Li N. Specific gene-regulation networks during the pre-implantation development of the pig embryo as revealed by deep sequencing. BMC Genomics. 2014 Jan 3;15(1):4. doi: 10.11861471-2164-15-4. (*Corresponding author)

11. Cao S, Loh K, Pei Y, Zhang W, Han J*. Overcoming barriers to the clinical utilization of iPSCs: reprogramming efficiency, safety and quality. Protein Cell. 2012 Nov;3(11):834-45. (*Corresponding author)

12. Han X*, Han J*, Ding F, Cao S, Lim SS, Dai Y, Zhang R, Zhang Y, Lim B, Li N. Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells. Cell Research. 2011, 21(10):1509-12. (*These authors contributed equally to this work.)

13. Jianyong Han, Ping Yuan , Henry Yang, Jinqiu Zhang, Junliang Tay, , Boon Seng Soh, Pin Li, Siew Lan Lim, Cao Suying, Yuriy Orlov, Thomas Luftkin, Huck-Hui Ng, Wai-Leong Tam, Bing Lim. Tbx3 improves the germ-line competency of induced pluripotent stem cells. Nature, 2010, 463(7284):1096-1100.

14. Heng JC, Feng B, Han J*, Jiang J, Kraus P, Ng JH, Orlov YL, Huss M, Yang L, Lufkin T, Lim B, Ng HH. Nuclear Receptor Nr5a2 replaces Oct4 in the Reprogramming of Murine Somatic Cells to pluripotent cells. Cell Stem Cell, 2010, 6(2):167-174. (*These authors contributed equally to this work.)

15. Yuan P*, Han J*, Guo G, Orlov YL, Huss M, Loh YH, Yaw LP, Robson P, Lim B, Ng HH. Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells. Genes Dev. 2009, 23(21):2507-20. (*These authors contributed equally to this work.)

16. Feng B, Jiang J, Kraus P, Ng JH, Heng JC, Chan YS, Yaw LP, Zhang W, Loh YH, Han J, Vega VB, Cacheux-Rataboul V, Lim B, Lufkin T, Ng HH. Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb. Nat Cell Biol. 2009, 11(2):197-203.

承擔項目

國家幹細胞重點研發項目課題

國家自然科學基金項目

人才項目：萬人計畫

重點實驗室自主重大項目

獲得榮譽

入職以來，入選中組部第三批青年千人、教育部新世紀優秀人才、科技部中青年科技領軍人才、中組部萬人計畫科技領軍人才等人才計畫。