陳文峰(中國農業大學生物學院副教授)

陳文峰，男，博士，中國農業大學微生物學與免疫學系副教授、碩士生導師，中國微生物學會會員，農業微生物學會會員，主要從事根瘤菌多樣性、系統發育、基因組學及根瘤菌與豆科植物共進化的研究。

基本介紹

• 中文名：陳文峰
• 畢業院校：中國農業大學
• 學位/學歷：博士
• 專業方向：微生物學與免疫學
• 任職院校：中國農業大學

人物經歷

1992.9 ～ 1996.7 河南師範大學生物系 本科

1996.9 ～ 1999.6 雲南大學微生物所 碩士

1999.9 ～ 2002.6 中國農業大學生物學院 博士

2002.10 ～2005.12 中國農業大學生物學院 講師

2006.1至今中國農業大學生物學院 副教授 碩士生導師

研究方向

共生固氮。重點解析影響根瘤菌與豆科植物共生固氮關係、共生固氮效率的內在和外在分子機制。

科研方面，圍繞根瘤菌與豆科植物共生固氮關係開展研究，涉及根瘤菌的多樣性、系統發育、生物地理學、分子生態學、根瘤菌與豆科植物的分子識別與信號交流，根瘤菌的適應性進化和代謝調控對共生固氮效率的影響，高活性根瘤菌菌劑研製及農業生產上的套用示範等研究。

主講課程

《微生物學》理論課和實驗課。

學術成果

發表論文

陳文峰,蔣凌月,徐麗華,姜成林.氣質聯用定量分析放線菌全細胞糖的研究.微生物學通報. 2000 , 27(6):416-420.

陳文峰,陳文新.我國豆科植物根瘤菌資源多樣性及套用基礎研究.生物學通報.2003.38(7):1-4.

陳文峰,陳文新.刺槐、黃檀、合歡根瘤菌的數值分類及16S rDNA-PCR RFLP研究.套用與環境生物學報. 2003. 41:53-58.

陳文峰,劉傑,陳文新.木本豆科植物根瘤菌資源及多樣性研究進展.科學技術與工程. 2004 , 4(11) , 954-959.

陳文峰,李洪波. 3株紫花苜蓿根瘤菌sinR基因的克隆、序列測定及系統發育分析.套用與環境生物學報. 2008. 14(2),240-243.

陳文峰. Sinorhizobium meliloti應譯為草木樨中華根瘤菌. 中國科技術語. 2008. 10(3):62-63.

劉曉雲,陳文峰,陳文新.根瘤菌的系統發育及其分類研究進展.微生物學通報. 2003, 29:73-76.

鄭君芳,陳文峰,劉桂榮,隋新華,朱萬孚,陳文新, R. N. Johnston , 劉樹林.根瘤菌基因組結構的多樣性及其系統發育的關係.微生物學通報.2003 , 30(4)：72-76.

陳文新,汪恩濤,陳文峰.根瘤菌-豆科植物共生多樣性與地理環境的關係.中國農業科學. 2004. 37(1):81-86.

陳文新,陳文峰.發揮生物固氮作用，減少化學氮肥用量.農業科技導報. 2004. 6(6):3-6.

谷峻,陳文峰等.根瘤菌的群體感應研究進展.微生物學通報. 2004. 31(6):110-114.

劉忠霞,陳文峰.教學用鞭毛染色的良好菌種－－燦爛類芽孢桿菌的鑑定.生物學通報. 2007. 42(6):48-50.

13.陳世瓊,陳文峰等. 16S rDNA PCR-RFLP法快速鑑定分離自濃縮蘋果汁生產線的脂環酸芽孢桿菌.中國食品學報. 2006. 6(2):99-102.

嚴雪瑞,陳文峰,陳文新,傅俊范,周如軍,隋新華.遼寧營口地區錦雞兒與樹錦雞兒根瘤菌遺傳多樣性的研究.瀋陽農業大學學報, 2007,38(1): 44-48.

嚴雪瑞,陳文峰,陳文新,傅俊范,薛彩雲,隋新華.小葉錦雞兒根瘤菌16S rDNA PCR-RFLP分析.華中農業大學學報，2007. 26(2):141-146.

雷霞,陳文峰,隋新華,陳文新.根瘤菌多相分類研究進展.微生物學雜誌. 2007. 27(6):77-80.

Tian Shen Tao*, Ying-yu Yue , Wen Xin Chen , Wen Feng Chen. Proposal of Nakamurella gen. nov. as a Substitute for the Bacterial Genus Microsphaera Yoshimi et al. 1996 , and Nakamurellaceae fam. nov. as a Substitute for the Illegitimate Bacterial Family Microsphaeraceae Rainey et al. 1997. International Journal of Systematic and Evolutionary Microbiology. 2004 , 54:999-1000.

Gui Xiang Peng , Zhi Yuan Tan , En Tao Wang , Barbara Reinhold-Hurek , Wen Feng Chen and Wen Xin Chen*. Identification of isolates from soybean nodules in Xinjiang Region as Sinorhizobium xinjiangense and genetic differentiation of S. xin, jiangense from Sinorhizobium fredii. International Journal of Systematic and Evolutionary Microbiology. 2002, 52:457–462.

Xue Rui Yan, Wen Feng Chen*, Jun Fan Fu, Yang Li Lu, Cai Yun Xue, Xin Hua Sui, Ying Li, En Tao, Wang and Wen Xin Chen. Mesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China. FEMS Microbiol Lett. 2007. 271:265-273.

Wen Feng Chen*, Su Hua Guan, Chun Tian Zhao, Xue Rui Yan, Chao Xin Man, En Tao Wang & Wen Xin Chen. Different Mesorhizobium species associated with Caragana carried similar symbiotic genes and had common host range. FEMS Microbiol Lett. 2008. 283:203-209.

Su Hua Guan, Wen Feng Chen*, En Tao Wang，Yang Li Lu，Xue Rui Yan, Xiao Xia Zhang and Wen Xin Chen. Mesorhizobium caraganae sp. nov., a novel rhizobial species nodulated with Caragana spp. in China. Int J Syst Evol Microbiol. 2008, 58: 2646 - 2653.

Wang FQ, Wang ET, Liu J, Chen Q, Sui XH, Chen WF, Chen WX*. Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China. Int J Syst Evol Microbiol. 2007 Jun;57(Pt 6):1192-1199.

Gu CT, Wang ET, Sui XH, Chen WF, Chen WX. Persity and geographical distribution of rhizobia associated with Lespedeza spp. in temperate and subtropical regions of China. Arch Microbiol. 2007 Oct;188(4):355-365.

Quan ZX*, Bae HS, Baek JH, Chen WF, Im WT, Lee ST. Rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2543-2539.

Yong Fa Zhang, En Tao Wang, Chang Fu Tian, Feng Qin Wang, Li Li Han, Wen Feng Chen, and Wen Xin Chen*. Bradyrhizobium elkanii, B. yuanmingense and B. japonicum are the main rhizobia associated with Vigna unguiculata and Vigna radiata in the subtropical region of China. FEMS Microbiol Lett. 2008. 258:146-154.

Ji Hong Li, En Tao Wang, Wen Feng Chen, Wen Xin Chen*. Genetic Persity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China. Soil Biology & Biochemistry. 2008. 40:238-246.

Man CX, Wang H, Chen WF, Sui XH, Wang ET, Chen WX* (2008) Diverse rhizobia associated with soybean grown in the subtropical and tropical regions of China. Plant Soil 310:77–87

Y. L. Lu, W. F. Chen*, L. L. Han, E. T. Wang, X. X. Zhang, W. X. Chen and S. Z. Han. Mesorhizobium shangrilense sp. nov., isolated from root nodules of Caragana spp. growing in Yunnan Province of China. Int J Syst Evol Microbiol. 2009.

Y. L. Lu, W. F. Chen, L. L. Han, E. T. Wang and W. X. Chen*. Rhizobium alkalisoli sp. nov., isolated from Caragana intermedia growing in saline-alkaline soils in the north of China. Int J Syst Evol Microbiol. 2009,

Lin DX, Chen WF，Wang FQ, Hu D, Wang ET, Sui XH and Chen WX*. Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes. Int J Syst Evol Microbiol. 2009, (contribute equally to this article)

Lu YL, Chen WF*, Wang ET, Guan SH, Yan XR, Chen WX.Genetic Persity and biogeography of rhizobia associated with Caragana species in three ecological regions of China. Syst Appl Microbiol. 2009 32: 351-361. (*Corresponding author).

Ren DW, Wang ET*, Chen WF, Sui XH, Zhang XX, Liu HC, Chen WX. Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China. Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1912-20.

Ren DW, Chen WF, Sui XH, Wang ET, Chen WX. Rhizobium vignae sp. nov., a symbiotic bacterium isolated from multiple legume species. Int J Syst Evol Microbiol. 2011 Mar;61(Pt 3):580-6.

Li QWang ET, Zhang YZ, Zhang YM, Tian CF, Sui XH, Chen WF, Chen WX. Diversity and biogeography of rhizobia isolated from root nodules of Glycine max grown in Hebei Province, China. Microb Ecol. 2011 May;61(4):917-31.

Li QWang ET, Chang YL, Zhang YZ, Zhang YM, Sui XH, Chen WF Chen WX*,. Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils. Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1981-8.

Zhang YM, Li Y Jr, Chen WF*, Wang ET, Tian CF, Li Q Zhang YZ, Sui XH, Chen WX. Biodiversity and biogeography of rhizobia associated with soybean plants grown in the North China Plain. Appl Environ Microbiol. 2011 Sep;77(18):6331-42.

Zhang YZ, Wang ET, Li M, Li QZhang YM, Zhang SJ, Jia XL, Zhang LH, Chen WF*, Chen WX. Effects of rhizobial inoculation, cropping systems and growth stages on endophytic bacterial community of soybean roots. Plant Soil, 2011, 347:147.

Li M, Li Y, Chen WF*, Sui XH, Li Y Jr, Li Y, Wang ET, Chen WX. Genetic diversity, community structure and distribution of rhizobia in the root nodules of Caragana spp. from arid and semi-arid alkaline deserts, in the north of China. Syst Appl Microbiol. 2012 Jun;35(4):239-45.

Zhang JJ, Liu TY, Chen WF*, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WX. Mesorhizobium muleiense sp. nov., nodulating with Cicer arietinum L. Int J Syst Evol Microbiol. 2012 Nov;62(Pt 11):2737-42.

Zhang JJ, Lou K, Jin X, Mao PH, Wang ET, Tian CF, Sui XH, Chen WF*. Distinctive Mesorhizobium populations associated with Cicer arietinum L. in alkaline soils of Xinjiang, China. Plant and Soil. 2012, Volume 353, Issue 1–2, pp 123–134.

Zhao CT, Wang ET, Zhang YM, Chen WF, Sui XH, Chen WX*, Liu HC, Zhang XX. Mesorhizobium silamurunense sp. nov., isolated from root nodules of Astragalus species. Int J Syst Evol Microbiol. 2012 Sep;62(Pt 9):2180-6.

Zhang YM, Li Y Jr, Chen WF*, Wang ET, Sui XH, Li QZhang YZ, Zhou YG, Chen WX. Bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (Glycine max L.) nodules. Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1951-7.

Zhang YZ, Chen WF*, Li M, Sui XH, Liu HC, Zhang XX, Chen WX. Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root. Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):359-63.

Liu TY, Li Y Jr, Liu XX, Sui XH, Zhang XX, Wang ET, Chen WX, Chen WF, Puławska J. Rhizobium cauense sp. nov., isolated from root nodules of the herbaceous legume Kummerowia stipulacea grown in campus lawn soil. Syst Appl Microbiol. 2012 Oct;35(7):415-20.

Zhang YM, Tian CF, Sui XH, Chen WF, Chen WX. Robust markers reflecting phylogeny and taxonomy of rhizobia. PLoS One. 2012;7(9):e44936.

Li Y, Tian CF, Chen WF, Wang L, Sui XH, Chen WX. High-resolution transcriptomic analyses of Sinorhizobium sp. NGR234 bacteroids in determinate nodules of Vigna unguiculata and indeterminate nodules of Leucaena leucocephala. PLoS One. 2013 Aug 2;8(8):e70531.

Wang YC, Wang F, Hou BC, Wang ET, Chen WF*, Sui XH, Chen WX, Li Y, Zhang YB. Proposal of Ensifer psoraleae sp. nov., Ensifer sesbaniae sp. nov., Ensifer morelense comb. nov. and Ensifer americanum comb. nov. Syst Appl Microbiol. 2013 Oct;36(7):467-73.

Wang JY, Wang R, Zhang YM, Liu HC, Chen WF, Wang ET, Sui XH, Chen WX. Bradyrhizobium daqingense sp. nov., isolated from soybean nodules. Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):616-24.

Ji ZJ, Wang FM, Wang SG, Yang SH, Guo R, Tang RY, Chen WX, Chen WF. [Symbiotic matching between soybean cultivar Luhuang No. 1 and different rhizobia]. Ying Yong Sheng Tai Xue Bao. 2014 Dec;25(12):3573-9. Chinese.

Zhang JJ, Yu T, Lou K, Mao PH, Wang ET, Chen WF*, Chen WX. Genotypic alteration and competitive nodulation of Mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils. Syst Appl Microbiol. 2014 Oct;37(7):520-4.

Mnasri B, Liu TY, Saidi S, Chen WF, Chen WX, Zhang XX, Mhamdi R. Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris. Int J Syst Evol Microbiol. 2014 May;64(Pt 5):1501-6.

Jiao YS, Liu YH, Yan H, Wang ET, Tian CF, Chen WX, Guo BL, Chen WF*. Rhizobial Diversity and Nodulation Characteristics of the Extremely Promiscuous Legume Sophora flavescens. Mol Plant Microbe Interact. 2015 Dec;28(12):1338-52.

Jiao YS, Yan H, Ji ZJ, Liu YH, Sui XH, Zhang XX, Wang ET, Chen WX, Chen WF*. Phyllobacterium sophorae sp. nov., a symbiotic bacterium isolated from root nodules of Sophora flavescens. Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):399-406.

Jiao YS, Yan H, Ji ZJ, Liu YH, Sui XH, Wang ET, Guo BL, Chen WX, Chen WF. Rhizobium sophorae sp. nov. and Rhizobium sophoriradicis sp. nov., nitrogen-fixing rhizobial symbionts of the medicinal legume Sophora flavescens. Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):497-503.

Yan H, Yan J, Sui XH, Wang ET, Chen WX, Zhang XX, Chen WF*. Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine. Int J Syst Evol Microbiol. 2016 Sep;66(8):2910-6.

Yan H, Ji ZJ, Jiao YS, Wang ET, Chen WF*, Guo BL, Chen WX. Genetic diversity and distribution of rhizobia associated with the medicinal legumes Astragalus spp. and Hedysarum polybotrys in agricultural soils. Syst Appl Microbiol. 2016 Mar;39(2):141-9.

Chen WH, Yang SH, Li ZH, Zhang XX, Sui XH, Wang ET, Chen WX, Chen WF*. Ensifer shofinae sp. nov., a novel rhizobial species isolated from root nodules of soybean (Glycine max). Syst Appl Microbiol. 2017 Apr;40(3):144-149.

Ji ZJ, Yan H, Cui QG, Wang ET, Chen WF, Chen WX. Competition between rhizobia under different environmental conditions affects the nodulation of a legume. Syst Appl Microbiol. 2017 Mar;40(2):114-119.

Yan J, Yan H, Liu LX, Chen WF, Zhang XX, Verástegui-Valdés MM, Wang ET, Han XZ. Rhizobium hidalgonense sp. nov., a nodule endophytic bacterium of Phaseolus vulgaris in acid soil. Arch Microbiol. 2017 Jan;199(1):97-104.

Yan H, Xie JB, Ji ZJ, Yuan N, Tian CF, Ji SK, Wu ZY, Zhong L, Chen WX, Du ZL, Wang ET, Chen WF*. Evolutionarily Conserved nodE, nodO, T1SS, and Hydrogenase System in Rhizobia of Astragalus membranaceus and Caragana intermedia. Front Microbiol. 2017 Nov 20;8:2282. doi: 10.3389fmicb.2017.02282.

Yan J, Li Y, Yan H, Chen WF, Zhang X, Wang ET, Han XZ, Xie ZH. Agrobacterium salinitolerans sp. nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina. Int J Syst Evol Microbiol. 2017 Jun;67(6):1906-1911.

Yan J, Li Y, Han XZ, Chen WF, Zou WX, Xie Z, Li M. Agrobacterium deltaense sp. nov., an endophytic bacteria isolated from nodule of Sesbania cannabina. Arch Microbiol. 2017 Sep;199(7):1003-1009.

Zhang JJ*, Chen Y, Philippe de L, Singh, RP, Wang, ET, Chen WF. Mesorhizobium muleiense and Mesorhizobium gsp. nov. are symbionts of Cicer arietinum L. in alkaline soils of Gansu, Northwest China. Plant and Soil. 2017, Volume 410, Issue 1–2, pp 103–112.

Yan J*, Chen WF, Han XZ, Wang ET, Zou WX, Zhang ZM. Genetic diversity of indigenous soybean-nodulating rhizobia in response to locally-based long term fertilization in a Mollisol of Northeast China. World Journal of Microbiology and Biotechnology. 2017, 33:6.

Liu YH, Wang ET, Jiao YS, Tian CF, Wang L, Wang ZJ, Guan JJ, Singh RP, Chen WX, Chen WF*.Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max). Microbiol Res. 2018 Sep;214:19-27.

Yang SH, Chen WH, Wang ET, Chen WF*, Yan J, Han XZ, Tian CF, Sui XH, Singh RP, Jiang GM, Chen WX. Rhizobial biogeography and inoculation application to soybean in four regions across China. J Appl Microbiol. 2018 Sep;125(3):853-866.

Hu Y, Jiao J, Liu LX, Sun YW, Chen WF, Sui XH, Chen WX, Tian CF*. Evidence for Phosphate Starvation of Rhizobia without Terminal Differentiation in Legume Nodules. Mol Plant Microbe Interact. 2018 Aug 20:MPMI02180031R. doi: 10.1094MPMI-02-18-0031-R.

Liu YH, Jiao YS, Liu LX, Wang D, Tian CF, Wang ET, Wang L, Chen WX, Wu SY, Guo BL, Guan ZG, Poinsot V, Chen WF*. Nonspecific Symbiosis Between Sophora flavescens and Different Rhizobia. Mol Plant Microbe Interact. 2018 Feb;31(2):224-232.

Zhang JJ*, Guo C, Chen WF, de Lajudie P, Zhang ZY, Shang YM, Wang ET. Mesorhizobium wenxiniae sp. nov., isolated from chickpea (Cicer arietinum L.) in China. 20 April 2018, International Journal of Systematic and Evolutionary Microbiology 68: 1930-193.

Zhang JJ*, Guo C, Chen WF, Shang YM, Lajudie Philippe de, Yang X, Mao PH, Zheng JQ, Wang ET. Dynamic succession of chickpea rhizobia over years and sampling sites in Xinjiang, China. Plant and Soil, 2018, 425 (1-2), p. 241-251.

陳文峰. 在教學實踐中提高青年微生物學教師的教學能力. 教育教學改革實踐與探索. 2004.

陳文峰, 王穎, 袁紅莉等. 高等農業院校農業微生物學立體開放實驗和系統建設.微生物教學與科研新進展. 2005.

關國華， 陳文峰， 李穎等. 新版的微生物生理學;. 微生物教學與科研新進展. 2005.

王穎, 袁紅莉, 陳文峰等.農業微生物學課程的量體裁衣式教學的探討. 微生物教學與科研新進展. 2005.

王穎, 李穎, 陳文峰.微生物生物學教學改革幾點嘗試. 教育教學改革實踐與探索， 2005，2：92-94.

陳文峰, 袁紅莉, 王穎, 李寶珍. 農業微生物學開放實驗課選課系統的設計與實現. 教育教學改革實踐與探索. 2006.

陳文峰, 關國華, 李穎, 袁紅莉. 多角度、多層次提升本科生的教學與管理水平－－上海交通大學、復旦大學、浙江大學教學考察總結與體會. 第11屆微生物學教學與科研研討會論文集. 2007. 中國新鄉.

陳文峰, 袁紅莉, 李寶珍, 王穎. 全方位推進農業微生物學精品課程建設。教育教學改革實踐與探索（迎評專輯），中國農大出版社，2007，9，p45-47.

劉忠霞, 陳文峰*. 微生物實驗教學用鞭毛染色的良好菌種－－燦爛類芽孢桿菌的鑑定. 生物學通報. 2007. 42(6):48-50. （*通訊作者）

陳文峰. 充分發揮多媒體課件的優勢提高課堂教學效果. 教育教學改革實踐與探索. 2009年4月出版發行. 136-138.

袁紅莉, 李寶珍, 陳文峰等其他11名作者. 精品課程《農業微生物學》階段性建設成果. 教育教學改革實踐與探索. 2009年4月出版發行. 95-97.

編著教材

《原核生物系統學》副主編，化學工業出版社。2007. （主編：陶天申，東秀珠，楊瑞馥）

《中國根瘤菌》副主編，科學出版社，2011. 主編：陳文新，汪恩濤；副主編：陳文峰、田長富。

承擔項目

在研主持國家自然科學基金面上項目：不同根瘤菌與苦參的混雜性共生機制研究，2018-2021.

專利發明

1、菌劑雙聯袋(豆樂寶)，專利號ZL2016302130298。

2、一株根瘤菌及其在檸條育苗中的套用的製作方法，專利號201310292237.7。

獲獎記錄

2008年度高等學校科學研究優秀成果獎（科學技術）：17屬豆科植物根瘤菌遺傳多樣性研究及7個根瘤菌新種的發表，獲獎人：陳文新,陳文峰,隋新華,李穎,韋革宏,王風芹,韓素貞,劉傑,劉曉雲,嚴雪瑞。