陳嘉瑜

陳嘉瑜，北京協和醫學院博士，同濟大學生命科學與技術學院副教授，博士生導師，國際幹細胞協會會員。

基本介紹

  • 中文名：陳嘉瑜
  • 畢業院校：北京協和醫學院
  • 學位/學歷：博士
  • 專業方向：細胞命運轉變過程中的表觀遺傳調控機制研究
  • 職務：同濟大學博士生導師
人物經歷,榮譽,學術研究,發表論文,

人物經歷

2017.12-至今同濟大學生命科學與技術學院，副教授
2014.10-2017.11同濟大學生命科學與技術學院，助理教授
2014.2-2014.10 同濟大學轉化醫學高等研究院，助理研究員
2009.9-2014.1 北京協和醫學院，北京生命科學研究所，生物化學與分子生物學博士
2004.9-2009.6 復旦大學，上海醫學院，醫學學士

榮譽

上海市晨光計畫項目獲得者(2016)，上海市青年科技啟明星（A類）獲得者(2018)，上海市人才發展資金資助計畫獲得者（2018），中國科協青年人才托舉項目獲得者（2018）。

學術研究

陳嘉瑜博士主要進行細胞命運轉變過程中的表觀遺傳調控機制研究，以及基於CRISPR/Cas9基因編輯技術的疾病模型構建的研究。首次證明了DNA羥甲基化酶Tet1及DNA羥甲基化調控細胞重編程的機制及其對細胞多能性的影響，研究成果在Cell Stem Cell雜誌以封面研究發表，並被評為2013年度最佳論文之一。2018年，合作揭示了Stella參與卵子發生過程中DNA甲基化模式建立的機制，並首次在體內證實了DNMT1可以作為起始性DNA甲基化轉移酶，打破了教科書里關於DNMT1隻是維持性DNA甲基化轉移酶的論斷，研究成果發表在Nature雜誌。此外，陳嘉瑜博士以主要骨幹身份參與科技部“國家重點研發計畫幹細胞及轉化研究重點專項”及“國家重大科學研究計畫（973）”各一項，主持國家自然科學基金“面上項目”一項，結題國家自然科學基金“青年項目”一項。近幾年，陳嘉瑜博士在Nature，Cell Stem Cell，Cell Research和Stem Cell Reports等國際知名學術期刊發表相關成果30餘篇（SCI），其中第一作者（含共同）或通訊作者論文（含共同）總計12篇。此外，陳嘉瑜博士培養的多位碩士或博士研究生獲得研究生國家或校級獎學金。

發表論文

  2. He W#, Chen J#*, Gao S*. Mammalian haploid stem cells: establishment, engineering and applications. Cell Mol Life Sci.2019 Mar 19. doi: 10.1007/s00018-019-03069-6. [Epub ahead of print] Review. (#co-first authors, *Co-correspondence)
  3. Li Y#, Zhang Z#, Chen J#, Liu W, Lai W, Liu B, Li X, Liu L, Xu S, Dong Q, Wang M, Duan X, Tan J, Zheng Y, Zhang P, Fan G, Wong J, Xu G, Wang Z, Wang H, Gao S, Zhu B*. Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1. Nature. 2018 Dec;564(7734):136-140. doi: 10.1038/s41586-018-0751-5. Epub 2018 Nov 28. (#co-first authors)
  4. 康嵐，陳嘉瑜，高紹榮. 中國細胞重編程和多能幹細胞研究進展. 遺傳,2018, 40 (10): 825-840. doi: 10.16288/j.yczz.18-209
  5. Li J#, Shen S#, Chen J#, Liu W, Li X, Zhu Q, Wang B, Chen X, Wu L, Wang M, Gu L, Wang H, Yin J, Jiang C*, Gao S*. Accurate annotation of accessible chromatin in mouse and human primordial germ cells. Cell Research. 2018 Nov;28(11):1077-1089. doi: 10.1038/s41422-018-0096-5. Epub 2018 Oct 10. (#co-first authors)
  6. Gao H#, Gao R#, Zhang L#, Xiu W, Zang R, Wang H, Zhang Y, Chen J*,Gao Y*, Gao S*. Esrrb plays important roles in maintaining self-renewal of trophoblast stem cells (TSCs) and reprogramming somatic cells to induced TSCs. Journal of Molecular Cell Biology.2018 Oct 9. doi: 10.1093/jmcb/mjy054. (*Co-correspondence)
  7. He W#, Zhang X#, Zhang Y, Zheng W, Xiong Z, Hu X, Wang M, Zhang L, Zhao K, Qiao Z, Lai W, Lv C, Kou X, Zhao Y, Yin J, Liu W, Jiang Y, Chen M, Xu R, Le R, Li C, Wang H, Wan X, Wang H, Han Z, Jiang C*, Gao S*, Chen J*. Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b. Stem Cell Reports.2018 Feb 13;10(2):477-493. doi: 10.1016/j.stemcr.2017.12.024. Epub 2018 Jan 27. (*Co-correspondence)
  8. Liu W#, Bai D#, Chen J*, Gao S*. Additive-effect pattern of both ZP2 and ZP3 in human and mouse. Hum Genet.2017 Nov 1. doi: 10.1007/s00439-017-1848-x. (*Co-correspondence)
  9. Liu W#, Li K#, Bai D#, Yin J#, Tang Y, Chi F, Zhang L, Wang Y, Pan J, Liang S, Guo Y, Ruan J, Kou X, Zhao Y, Wang H, Chen J*, Teng X*, Gao S*. Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility. Hum Genet. 2017 Aug;136(8):975-985. doi: 10.1007/s00439-017-1822-7. Epub 2017 Jun 24.(*Co-correspondence)
  10. Gao R#, Xiu W#, Zhang L, Zang R, Yang L, Wang C, Wang M, Wang M, Yi L, Tang Y, Gao Y, Wang H, Xi J, Liu W, Wang Y, Wen X, Yu Y, Zhang Y, Chen L*, Chen J*, Gao S*. Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state. Biomaterials.2017 Mar;119:53-67. doi: 10.1016/j.biomaterials.2016.12.007. (*Co-correspondence)
  11. Xu K#, Chen X#, Yang H, Xu Y, He Y, Wang C, Huang H, Liu B, Liu W, Li J, Kou X, Zhao Y, Zhao K, Zhang L, Hou Z, Wang H, Wang H, Li J, Fan H, Wang F, Gao Y, Zhang Y,Chen J*, Gao S*. Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes. J Biol Chem.2017 Feb 3;292(5):1798-1807. doi: 10.1074/jbc.M116.767061. Epub 2016 Dec 28. (*Co-correspondence)
  12. Chen J#*, Gao Y#, Huang H, Xu K, Chen X, Jiang Y, Li H, Gao S, Tao Y, Wang H, Zhang Y, Wang H, Cai T, Gao S*. The combination of Tet1 with Oct4 generates high-quality mouse induced pluripotent stem cells (iPSCs). Stem Cells.2015 Mar;33(3):686-98. doi: 10.1002/stem.1879. (#co-first authors, *Co-correspondence)
  13. Liu X#, Chen J#, Liu W, Li X, Chen Q, Liu T, Gao S*, Deng M*.The fused in sarcoma protein forms cytoplasmic aggregates in motor neurons derived from integration-free induced pluripotent stem cells generated from a patient with familial amyotrophic lateral sclerosis carrying the FUS-P525L mutation. Neurogenetics. 2015 Jul;16(3):223-31. doi: 10.1007/s10048-015-0448-y. Epub 2015 Apr 26. (#co-first authors)
  14. Gao Y#, Chen J#, Li K, Wu T, Huang B, Liu W, Kou X, Zhang Y, Huang H, Jiang Y, Yao C, Liu X, Lu Z, Xu Z, Kang L, Chen J, Wang H, Cai T and Gao S. Replacement of Oct4 by Tet1 during iPSC induction reveals an important role of DNA methylation and hydroxymethylation in reprogramming. Cell Stem Cell, 12(4):453-469, 2013.(Cover Story; Previewed by Cell Stem Cell; Selected as Best of 2013 by Cell Stem Cell)(#co-first authors)
  15. Wei X#, Guo J#, Li Q, Jia Q, Jing Q, Li Y, Zhou B, Chen J, Gao S, Zhang X, Jia M, Niu C, Yang W, Zhi X, Wang X, Yu D, Bai L, Wang L, Na J, Zou Y, Zhang J, Zhang S, Meng D*. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv.2019 Mar 13;5(3):eaau7887. doi: 10.1126/sciadv.aau7887. eCollection 2019 Mar
  16. Zheng R#, Li Z#, He F#, Liu H*, Chen J, Chen J, Xie X, Zhou J, Chen H, Wu X, Wu J, Chen B, Liu Y, Cui H, Fan L, Sha W, Liu Y, Wang J, Huang X, Zhang L, Xu F, Wang J, Feng Y, Qin L, Yang H, Liu Z, Cui Z, Liu F, Chen X, Gao S, Sun S*, Shi Y*, Ge B*. Genome-wide association study identifies two risk loci for tuberculosis in Han Chinese. Nat Commun. 2018 Oct 4;9(1):4072. doi: 10.1038/s41467-018-06539-w.
  17. Gao R#, Wang C#, Gao Y#, Xiu W, Chen J, Kou X, Zhao Y, Liao Y, Bai D, Qiao Z, Yang L, Wang M, Zang R, Liu X, Jia Y, Li Y, Zhang Y, Yin J, Wang H, Wan X, Liu W*, Zhang Y*, Gao S*. Inhibition of Aberrant DNA Re-methylation Improves Post-implantation Development of Somatic Cell Nuclear Transfer Embryos. Cell Stem Cell. 2018 Sep 6;23(3):426-435.e5. doi: 10.1016/j.stem.2018.07.017. Epub 2018 Aug 23.
  18. Guo X#, Xu Y#, Wang Z, Wu Y, Chen J, Wang G, Lu C, Jia W, Xi J, Zhu S, Jiapaer Z, Wan X, Liu Z, Gao S, Kang J*. A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis. Cell Stem Cell.2018 Jun 1;22(6):893-908.e6. doi: 10.1016/j.stem.2018.04.013. Epub 2018 May 10.
  19. Fang L#, Teng H#, Wang Y#, Liao G#, Weng L, Li Y, Wang X, Jin J, Jiao C, Chen L, Peng X, Chen J, Yang Y, Fang H, Han D, Li C, Jin X, Zhang S, Liu Z, Liu M, Wei Q, Liao L, Ge X, Zhao B, Zhou D, Qin HL, Zhou J, Wang P. SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Cancer Cell.2018 Jul 9;34(1):103-118.e9. doi: 10.1016/j.ccell.2018.06.002. Epub 2018 Jun 28
  20. Wang C#, Liu X#, Gao Y#, Yang L#, Li C, Liu W ,Chen C, Kou X, Zhao Y, Chen J, Wang Y, Le R, Wang H, Duan T, Zhang Y, Gao S.(2018) Reprogramming of H3K9me3-dependent heterochromatin during mammalian embryo development. Nature Cell Biology. 2018 May;20(5):620-631. doi: 10.1038/s41556-018-0093-4. Epub 2018 Apr 23
  21. Zhuang Q, Li W, Benda C, Huang Z, Ahmed T, Liu P, Guo X, Ibañez DP, Luo Z, Zhang M, Abdul MM, Yang Z, Yang J, Huang Y, Zhang H, Huang D, Zhou J, Zhong X, Zhu X, Fu X, Fan W, Liu Y, Xu Y, Ward C, Khan MJ, Kanwal S, Mirza B, Tortorella MD, Tse HF, Chen J, Qin B, Bao X, Gao S, Hutchins AP, Esteban MA. NCoR/SMRT co-repressors cooperate with c-MYC to create an epigenetic barrier to somatic cell reprogramming. Nat Cell Biol.2018 Mar 12. doi: 10.1038/s41556-018-0047-x.
  22. Zhu J, Wang K, Li T, Chen J, Xie D, Chang X, Yao J, Wu J, Zhou Q, Jia Y, Duan T. Hypoxia-induced TET1 facilitates trophoblast cell migration and invasion through HIF1α signaling pathway.Sci Rep.2017 Aug 14;7(1):8077. doi: 10.1038/s41598-017-07560-7.
  23. Li Y, Hai Y, Chen J, Liu T. Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells.J Vis Exp. 2017 Jul 18;(125). doi: 10.3791/55722.
  24. Li H, Lai P, Jia J, Song Y, Xia Q, Huang K, He N, Ping W, Chen J, Yang Z, Li J, Yao M, Dong X, Zhao J, Hou C, Esteban MA, Gao S, Pei D, Hutchins AP, Yao H. RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Cell Stem Cell.2017 Jan 11. pii: S1934-5909(16)30458-1. doi: 10.1016/j.stem.2016.12.002.
  25. Liu X, Wang C, Liu W, Li J, Li C, Kou X, Chen J, Zhao Y, Gao H, Wang H, Zhang Y, Gao Y, Gao S (2016). Distinct features of H3K4me3 and H3K27me3 chromatin domains in pre-implantation embryos. Nature.2016 Sep 14;537(7621):558-562. doi: 10.1038/nature19362.
  26. Xiong J, Zhang Z,Chen J, Huang H, Xu Y, Ding X, Zheng Y, Nishinakamura R, Xu G, Wang H, Chen S, Gao S and Zhu B (2016). Cooperative Action between SALL4A and TET Proteins in Stepwise Oxidation of 5-Methylcytosine. Molecular Cell, http://dx.doi.org/10.1016/j.molcel.2016.10.013.
  27. Zhang W, Xia W, Wang Q, Towers AJ, Chen J, Gao R, Zhang Y, Yen CA, Lee AY, Li Y, Zhou C, Liu K, Zhang J, Gu TP, Chen X, Chang Z, Leung D, Gao S, Jiang YH, Xie W. (2016). Isoform Switch of TET1 Regulates DNA Demethylation and Mouse Development. Molecular Cell. http://dx.doi.org/10.1016/j.molcel.2016.10.030.
  28. Xiong Z, Yi L, Cao D, He W, Chen J, Gao S, Sun X (2016). Dravet syndrome with autism inherited from a paternal mosaic heterozygous mutation on SCN1A. J Neurol Sci. 2016 Oct 15;369:53-6. doi: 10.1016/j.jns.2016.07.038.
  29. Li, Y., T. Liu, N. Van Halm-Lutterodt,J. Chen, Q. Su and Y. Hai (2016). "Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair." Stem Cell Res Ther7(1): 31.
  30. Illich, D. J., M. Zhang, A. Ursu, R. Osorno, K. P. Kim, J. Yoon, M. J. Arauzo-Bravo, G. Wu, D. Esch, D. Sabour, D. Colby, K. S. Grassme, J. Chen, B. Greber, S. Hoing, W. Herzog, S. Ziegler, I. Chambers, S. Gao, H. Waldmann and H. R. Scholer (2016). "Distinct Signaling Requirements for the Establishment of ESC Pluripotency in Late-Stage EpiSCs." Cell Rep. 2016 Apr 14. pii: S2211-1247(16)30362-X. doi: 10.1016/j.celrep.2016.03.073.
  31. Xu, S., X. Zhu, H. Li, Y. Hu, J. Zhou, D. He, Y. Feng, L. Lu, G. Du, Y. Hu, T. Liu, Z. Wang, G. Ding, J. Chen, S. Gao, F. Wu, Z. Xue, Y. Li and G. Fan (2016). "The 14th Ile residue is essential for Leptin function in regulating energy homeostasis in rat." Sci Rep.2016 Jul 5;6:28508. doi: 10.1038/srep28508.
  32. Yang, Y., X. Zhang, L. Yi, Z. Hou, J. Chen, X. Kou, Y. Zhao, H. Wang, X. F. Sun, C. Jiang, Y. Wang and S. Gao (2016). Naive Induced Pluripotent Stem Cells Generated From beta-Thalassemia Fibroblasts Allow Efficient Gene Correction With CRISPR/Cas9. Stem Cells Transl Med5(2): 267.
  33. Wei, T., W. Chen, X. Wang, M. Zhang, J. Chen, S. Zhu, L. Chen, D. Yang, G. Wang, W. Jia, Y. Yu, T. Duan, M. Wu, H. Liu, S. Gao and J. Kang (2015). An HDAC2-TET1 switch at distinct chromatin regions significantly promotes the maturation of pre-iPS to iPS cells. Nucleic Acids Res doi:10.1093/nar/gkv430.
  34. Liu X, Chen J, Li X, Gao S, Deng M. (2014) Generation of induced pluripotent stem cells from amyotrophic lateral sclerosis patient carrying SOD1-V14M mutation. Natl Med J China, DOI:10.3760/cma.j.issn.0376-2491. 2014.27.017.
  35. Chang G, Gao S, Hou X, Xu Z, Liu Y, Kang L, Tao Y, Liu W, Huang B, Kou X,Chen J, An L, Miao K, Di K, Wang Z, Tan K, Cheng T, Cai T, Gao S*, Tian J*. (2013) High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells. Cell Research, doi:10.1038/cr.2013.173.
  36. Jiao J, Yang Y, Shi Y, Chen J, Gao R, Fan Y, Yao H, Liao W, Sun X* and Gao S*. (2013) Modeling Dravet syndrome using induced pluripotent stem cells (iPSCs) and directly converted neurons. Human Molecular Genetics, 22(21):4241-4252.
  37. Wang Y, Zheng C, Jiang Y, Zhang J, Chen J,YaoC, Zhao Q, Liu S, Chen K, Du J, Yang Z, Gao S*. (2012) Genetic correction of β-thalassemia patient-specific iPS cells and its use in improving hemoglobin production in irradiated SCID mice. Cell Research, 22(4):637-48.
  38. Yang P, Wang Y,Chen J, Li H, Kang L, Zhang Y, Chen S, Zhu B*, Gao S*. (2011) Rcor2 is a Subunit of the LSD1 Complex that Regulates ES Cell Property and Substitutes for Sox2 in Reprogramming Somatic Cells to Pluripotency. Stem Cells, 29(5):791-801.
  39. Cai X, Zhang J, Chen M, Wu Y, Wang X, Chen J, Zhang J, Shen X, Qu D, Jiang H.(2011) The effect of the potential PhoQ histidine kinase inhibitors on Shigella flexneri virulence.PLoS One, 6(8):e23100, 2011.
  40. 陳嘉瑜, 蔡霞, 瞿滌; 沙門菌PhoP-PhoQ雙組分信號轉導系統[J]; 微生物與感染; 2010年04期.

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