基本介紹
- 中文名:金雙俠
- 國籍:中國
- 出生日期:1979.2
- 畢業院校:華中農業大學
- 民族:漢
- 性別:男
基本概況,個人簡介,科研項目,發明專利及獲獎情況獲獎情況,獲獎情況,申報六項發明專利,發表的代表性論文:,
基本概況
姓名:金雙俠
碩博導:博導
職稱:教授
開設課程:植物生物技術,作物生產實習,育種學實習
研究方向:棉花生物技術,細胞質基因組及生物技術
個人簡介
華中農業大學植物科學技術學院作物遺傳育種專業博士,教授,博士生導師,作物遺傳改良國家重點實驗室PI。2001-2006年,在華中農業大學植物科學技術學院攻讀碩士、博士學位,獲農學博士學位. 2007.10-2012.4在美國賓夕法尼亞大學Henry Daniell教授指導下進行博士後研究,研究方向為植物葉綠體轉化。目前主要從事棉花生物技術研發(基因編輯等);棉花與害蟲互作分子機制及抗蟲相關基因的克隆和高效表達載體(葉綠體轉化表達體系)的開發研究。
近年來以第一作者或通訊作者在植物學知名期刊Trends in Plant Science、New phytologist、Plant Physiology、Plant Biotechnology Journal等雜誌上發表30多篇植物抗蟲基因工程的學術文章,其中IF > 6的文章12篇,總引用次數1500多次。2018年入選Plant Biotechnology Journal雜誌(中科院雜誌分區1區,IF=6.3)副主編以及Journal of Cotton Research(棉花學報英文版)副主編 (高級編輯);2017年當選棉花基礎研究領域最權威的學術機構-國際棉花基因組協會(ICGI)共同主席,是該學會最年輕的共同主席。先後主持國家自然科學基金面上項目、轉基因專項、十三五國家重點研發專項等國家級課題8項。
科研項目
十三五國家重點研發專項 子課題 主持 2016-2020
轉基因專項子課題 主持 2016-2020
湖北省自然科學基金重點項目(傑青項目) 主持,2015.1-2016.12
國家自然科學基金 煙粉虱與棉花互作的分子機理研究 主持,2014-2017
國家自然科學基金 植物凝集素基因在棉花中的功能分析,主持,2012-2015
新疆建設兵團博士資金項目 轉基因抗病蟲、抗逆聚合雜交棉新組合選育技術研究,主持,2007-2009
國家自然科學基金 棉花外源基因葉綠體轉化體系的建立及其套用(30771368),主持,2008-2010
教育部博士點新教師基金 以gfp基因為報告基因監測轉基因棉花花粉漂移(2007.0504087),主持,2008-2010
國家自然科學基金重大國際合作項目 Development of strong pest-resistance cotton via transforming
plastid and its application in breeding,主要參加者,2009-2011;
國家自然科學基金 利用基因誘捕構建棉花功能基因組研究突變群體,主要參加,2005-2007
發明專利及獲獎情況獲獎情況
獲獎情況
【棉花種質創新及強優勢雜交新品種選育與套用】 國家科技進步二等獎 2013年
【棉花體細胞離體遺傳操作技術與控制理論】 教育部自然科學二等獎
湖北省優秀博士學位論文(第10批)獲獎者,2008年
湖北省優秀本科論文指導獎,2008年。指導的2007界本科生張碧瑤,其論文:新疆棉花高體細胞胚胎髮生
能力基因型的篩選,獲得湖北省優秀本科學位論文
“棉花分子育種技術體系建立與套用”獲2008年湖北省技術發明二等獎
獲第十、十一屆湖北省自然科學優秀論文一等獎2次
申報六項發明專利
超音波輔助農桿菌轉化棉花胚芽的方法,專利申請號:200710063650.0
一種分離棉花葉綠體DNA的方法,專利申請號:200710052665.7
利用苦豆子分離基因SaVP1培育耐高溫擬南芥的方法. 專利號 ZL 201310006089.8
利用花花柴KcNHX1 基因培育耐高溫擬南芥的方法 專利申請號: 201310160491.1
利用棉花GhPEPC基因創造棉花高油材料的方法 專利申請號:201510836230.6
一種提高棉花再生及轉化效率的方法及套用 專利申請號:201510836230.6
一種棉花基因的編輯方法 專利申請號 2016111884024
利用植物介導的RNAi技術創造棉花抗中黑盲蝽新種質的方法 專利申請號:2017102984371
一種DsRED2報告基因在棉花遺傳改良中的套用 專利申請號 201611113581.5
陸地棉基因組的精準高效編輯方法 專利申請號2018115777177
陸地棉基因組的精確高效編輯方法 專利申請號 201910290886.0
利用植物介導的RNAi創造棉花抗綠盲蝽種質的方法 專利申請號 2019100115879
發表的代表性論文:
1. Lei Qin, Jianying Li, Qiongqiong Wang, Zhongping Xu, Lin Sun, Muna Alariqi, Hakim Manghwar, Guanyin Wang, Bo Li, Xiao Ding, Hangping Rui, Huimei Huang, Tianliang Lu , Keith Lindsey,Henry Daniell, Xianlong Zhang, Shuangxia Jin*.High Efficient and Precise Base Editing of C·G to T·A in the Allotetraploid Cotton (Gossypium hirsutum) Genome Using a Modified CRISPR/Cas9 System.Plant Biotechnology Journal, 2019, (*通訊作者)(SCI IF:6.3)
2. Bo Li Hangping Rui Yajun Li Qiongqiong Wang Muna Alariqi Lei Qin Lin Sun Xiao Ding Fuqiu Wang Jiawei Zou Yanqing Wang Daojun Yuan Xianlong Zhang Shuangxia Jin * . Robust CRISPR/Cpf1(Cas12a) mediated genome editing in allotetraploid cotton (G.hirsutum). Plant Biotechnology Journal, 2019,(*通訊作者)(SCI IF:6.3)
3.Maojun Wang, Lili Tu, Daojun Yuan, De Zhu, Chao Shen, Jianying Li, Fuyan Liu, Liuling Pei, Pengcheng Wang, Guannan Zhao, Zhengxiu Ye, Hui Huang, Feilin Yan, Yizan Ma, Lin Zhang, Min Liu, Jiaqi You, Yicheng Yang, Zhenping Liu, Fan Huang, Baoqi Li, Ping Qiu, Qinghua Zhang, Longfu Zhu, Shuangxia Jin, Xiyan Yang, Ling Min, Guoliang Li, Ling-Ling Chen, Hongkun Zheng, Keith Lindsey, Zhongxu Lin, Joshua A. Udall & Xianlong Zhang.Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense. 2018, Nature Genetics
4.Li,Jianying;Manghwar,Hakim;Sun,Lin;Wang,Pengcheng;Wang,Guanying;Sheng, Hanyan;Zhang,Jie;Liu,Hao;Qin,Lei;Rui,Hangping;Li,Bo;Lindsey,Keith;Daniell,Henry; Jin, Shuangxia*;Zhang,Xianlong.Whole genome sequencing reveals rare off-target mutations and considerablein herentgeneticor/ and somaclonal variations in CRISPR-Cas9-editedcottonplants. 2018, Plant Biotechnology Journal, 17, 858-868 (*通訊作者) (SCI IF:6.3)
5.Hu Q, Min L, Yang X, Jin S, Zhang L, Li Y, Ma Y, Qi X, Li D, Liu H, Lindsey K, Zhu L, Zhang X. Laccase GhLac1 modulates broad-spectrum biotic stress tolerance via DAMP-triggered immunity. Plant Physiology, 2018, 176(2):1808-1823 ((SCI IF:5.95)
6.Jianying Li, Maojun Wang, Yajun Li, Qinghua Zhang, Keith Lindsey, Henry Daniell,Shuangxia Jin*, Xianlong Zhang. Multi-omics analyses reveal epigenomics basis for cotton somatic embryogenesis through successive regeneration acclimation (SRA) process, 2018,Plant Biotechnology Journal, 17,435-450(*通訊作者) (SCI IF:6.3)
7.Luo, Jing; Liang, Sijia; Li, Jianying; Xu, Zhongping; Li, Lun; Zhu, Bangqin; Li, Zhe; Lei, Chaoliang; Lindsey, Keith; Chen, Lizhen*; Jin, Shuangxia*; Zhang, Xianlong. A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double- stranded RNA (dsRNA) homologous to Fatty acyl-CoA reductase (FAR) in cotton. New Phytologist, 2017(*通訊作者) (SCI IF: 7.2)
8.Lin Sun, Muna Alariqi, YiZhu, Jianying Li, Zelin Li, Qing Wang, Yajun Li, Hangping Rui, Xianlong Zhang,Shuangxia Jin*. Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular b reeding,Crop Journal, 2018, 6(4): 366-376(*通訊作者) (SCI IF:2.6)
9.Wang, Pengcheng; Zhang, Jun; Sun, Lin; Ma, Yizan; Xu, Jiao; Liang, Sijia; Deng, Jinwu; Tan, Jiafu; Zhang, Qing; Tu, Lili; Daniell, Henry; Jin, Shuangxia*; Zhang, Xianlong*. High efficient multi-sites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system,Plant Biotechnology Journal, 2017,(*通訊作者) (SCI IF: 6.3)
9.Wang, Pengcheng; Zhang, Jun; Sun, Lin; Ma, Yizan; Xu, Jiao; Liang, Sijia; Deng, Jinwu; Tan, Jiafu; Zhang, Qing; Tu, Lili; Daniell, Henry; Jin, Shuangxia*; Zhang, Xianlong*. High efficient multi-sites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system,Plant Biotechnology Journal, 2017,(*通訊作者) (SCI IF: 6.3)
10.Maojun Wang, Lili Tu, Min Lin, Zhongxu Lin, Pengcheng Wang, Qingyong Yang, Zhengxiu Ye, Chao Shen, Jianying Li, Lin Zhang, Xiaolin Zhou, Xinhui Nie, Zhonghua Li, Kai Guo, Yizan Ma, Cong Huang, Shuangxia Jin, Longfu Zhu, Xiyan Yang, Ling Min, Daojun Yuan, Qinghua Zhang, Keith Lindsey & Xianlong Zhang,2017, Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication,Nature Genetics, 49(4):579-587
11. Jianying Li#, Lizhen Zhu#, Joe Hull, Sijia Liang, Henry Daniell, Shuangxia Jin*, Xianlong Zhang. Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly). Plant Biotechnology Journal, 2016. (SCI, IF:6.3)(通訊作者)
12.Zhongping Xu , Jingwen Li , Xiaoping Guo , Shuangxia Jin*, Xianlong Zhang*.Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference.Scientific Reports, 2016, (*通訊作者)(SCI IF: 5.2)
13.Shuangxia Jin and Henry Daniell.The Engineered Chloroplast Genome Just Got Smarter. Trends in Plant Science, 2015, 20(10):622-640 (SCI,IF:12.93)
14.Geng Tian, Linlin Cheng, Xuewei Qi, Zonghe Ge, Changying Niu, Xianlong Zhang, Shuangxia Jin* Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms. Int. J. Biol. Sci. 2015; 11(11):1296-1305.(SCI, IF:4.5)(通訊作者)
15.Jin Shuanxia,Singh, Dolendro,Li Lebin,Zhang, Xianlong and Daniell, Henry. Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthases genes in the insect gut and disrupts Helicoverpa armigera larval development and pupation. Plant Biotechnology Journal, 2015,13 (3), 435-446.(SCI, IF:6.3)
16.Shuangxia Jin and Henry Daniell.Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stress by reducing reactive oxygen species. Plant Biotechnology Journal, 2014 (12),1274-1285 . (SCI, IF:6.3)
17. Shuangxia Jin, Xianlong Zhang1, Henry Daniell. Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, lepidopteran insects, bacterial and viral pathogens.Plant Biotechnology Journal 2012,10(3):313-327 (Cover Story, IF 6.3).
18.Shuangxia Jin, Anderson Kanagaraj, Dheeraj Verma, Theo Lange, Henry Daniell.Release of Hormones from Conjugates: Chloroplast Expression of ß-glucosidase Results in Elevated Phytohormone Levels Associated with Significant Increase in Biomass and Protection from Aphids or Whiteflies Conferred by Sucrose Esters. Plant Physiology,2011,155,222-235.(SCI,IF:6.5)