金亞，清華大學化學系博士，現任廣東工業大學碩士生導師。
基本介紹
- 中文名：金亞
- 國籍：中國
- 職業：教師
- 畢業院校：清華大學
- 學位/學歷：不是
- 專業方向：分析化學
- 職務：廣東工業大學碩士生導師
教育經歷,工作經歷,教學活動,學術成果,承擔項目,著作,論文,
教育經歷
1997.09－2002.07：清華大學化學系，分析化學專業，獲博士學位
1992.09－1997.07：清華大學化學系，物理化學及儀器分析專業，獲學士學位
工作經歷
2017.3至今：廣東工業大學，生物醫藥研究院，教授
2012.10－2017.02：華南理工大學，生物科學與工程學院，副研究員/副教授
2005.04－2012.04：日本愛媛大學，理工學研究科，外國人研究員
2003.04－2005.03：日本愛媛大學理學部，日本學術振興會（JSPS）外國人特別研究員
教學活動
已畢業碩士研究生6名，現指導博士研究生1名，碩士研究生1名。
主講本科生必修課《生物化學及分子生物學》、《現代生化技術》；碩士研究生必修課《SCI文章寫作與優秀外文科技文章導讀》，碩士及博士研究生選修課《蛋白質組學分析技術及蛋白質組信息學》、《專業英語》等。
學術成果
承擔項目
在研主持項目：廣東工業大學引進人才項目1；已結題主持項目：國家自然科學基金青年科學基金項目1、華南理工大學中央高校基本科研業務費重點項目1、廣州市科技計畫項目1、廣東省發酵與酶工程重點實驗室開放基金項目2、華南理工大學中央高校基本科研業務費面上項目1。
著作
Takashi Manabe,Ya Jin,in: Hughes AB (Ed.),Amino acids, Peptides and Proteins in Organic Chemistry: Volume 5: Analysis and Function of Amino Acids and Peptides, Wiley-VCH Verlag GmbH & Co. KGaA , Weinheim 2011, Chapter 13 “Two-dimensional gel electrophoresis and protein/polypeptide assignment”, pp. 439-462.
論文
1.Meiling Wen,Ya Jin*, Hao Zhang, Xiaoou Sun, Yihe Kuai,Wen Tan*, Proteomic Analysis of Rat Cerebral Cortex in the Sub-Acute to Long-Term Phases of Focal Cerebral Ischemia-Reperfusion Injury. J. Proteome Res. 2019, online (DOI: 10.1021/acs.jproteome.9b00220).
2.Ya Jin*, Meiling Wen, Qi Yuan, Jun Zhang, Wen Tan*, Beneficial effects of Coomassie staining on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS.J. Chromatogr. B2019,1110-1111, 25-35.
3.Ya Jin*, Jun Zhang, Takashi Manabe, Wen Tan*, Comparison of the performance of 1D SDS-PAGE with nondenaturing 2DE on the analysis of proteins from human bronchial smooth muscle cells using quantitative LC-MS/MS.J. Chromatogr. B2019,1105, 193-202.
4.Meiling Wen,Ya Jin*, Takashi Manabe, Shumin Chen, Wen Tan*, A comparative analysis of human plasma and serum proteins by combining native PAGE, whole-gel slicing and quantitative LC-MS/MS: Utilizing native MS-electropherograms in proteomic analysis for discovering structure and interaction-correlated differences.Electrophoresis2017,38(24), 3111–3123.
5.Shumin Chen, Meiling Wen, Shujie Bu, Ahui Wang,Ya Jin*, Wen Tan*. Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and quantitative LC-MS/MS.Electrophoresis2016,37(23-24), 3126-3136.
6.Lixian Cheng, Hui Shi, Yan Jin, Xiaoxi Li, Jinshun Pan, Yimei Lai, Yan Lin,Ya Jin, Gaurab Roy, Allan Zhao, Fanghong Li. Adiponectin Deficiency Leads to Female Subfertility and Ovarian Dysfunctions in Mice.Endocrinology2016, 157(12), 4875-4887.
7.Ahui Wang, Xiaoou Sun, Qing Liu, Tingting Hu, Ting Han,Ya Jin*, Wen Tan*. The protective effects of Sildenafil on transverse aortic constriction induced cardiac hypertrophy in rats.Journal of Guangdong College of Pharmacy(China) 2016,32(4), 475-480.
8.Jie Wu, Yiguang Tian, Shanping Wang, Marco Pistolozzi,Ya Jin, Ting Zhou, Gaurab Roy,Ling Xu, Wen Tan. Design, synthesis and biological evaluation of bambuterol analogues as novel inhibitors of butyrylcholinesterase.Eur J Med Chem.2016,126, 61-71.
9.Ya Jin,Jin Chen, Ahui Wang, Jun Zhang, Shumin Chen, Takashi Manabe, Wen Tan*. Analysis of low-density lipoprotein-associated proteins using the method of digitized native protein mapping.Electrophoresis2016,37(14), 2063-2074.
10.Ya Jin, Qi Yuan, Jun Zhang, Takashi Manabe, Wen Tan. Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 2. Similarity search between protein maps for the analysis of protein complexes.Electrophoresis2015,36(17), 1991-2001.
11.Ya Jin, Jun Zhang, Qi Yuan, Takashi Manabe, Wen Tan. Proteomic analysis of cellular soluble proteins from human bronchial smooth muscle cells by combining nondenaturing micro 2DE and quantitative LC-MS/MS. 1. Preparation of more than 4000 native protein maps.Electrophoresis2015,36(15),1711-1723.
12.Jun Zhang,Ya Jin*, Yanrui Ye, Wen Tan. Technologies in G-protein-coupled receptor-ligand binding assays.Chinese Journal of Pharmaceutical Analysis2015,35(1),1-7.
13.Ya Jin, Shujie Bu, Jun Zhang, Qi Yuan, Takashi Manabe, Wen Tan. Native protein mapping and visualization of protein interactions in the area of human plasma high-density lipoprotein by combining nondenaturing micro 2DE and quantitative LC-MS/MS.Electrophoresis2014,35(14), 2055-2064.
14.Takashi Manabe,Ya Jin. Performance of non-denaturing micro 2-DE followed by third-dimension SDS-PAGE in the analysis ofEscherichia colisoluble proteins.Electrophoresis2011,32(2), 300-309.
15.Takashi Manabe,Ya Jin. Analysis ofE. colisoluble proteins by non-denaturing micro 2-DE/3-DE and MALDI-MS-PMF.Electrophoresis2010,31(16), 2740-2748.
16.Takashi Manabe,Ya Jin. Assignment of human plasma proteins on non-denaturing micro 2-DE gels using MALDI-MS.Seibutsu Butsuri Kagaku2010,54(1), 31-34.Japanese.
17.Ya Jin, Takashi Manabe. Analysis of polyethylene glycol-fractionated high-molecular-mass proteins in human plasma by non-denaturing micro 2-DE and MALDI-MS PMF.Electrophoresis2009;30(20), 3613-3621.
18.Ya Jin, Takashi Manabe. Differences in protein distribution between human plasma preparations, EDTA-plasma and heparin-plasma, analyzed by non-denaturing micro 2-DE and MALDI-MS PMF.Electrophoresis2009;30(6), 931-938.
19.Ya Jin, Takashi Manabe. Performance of agarose IEF gels as the first dimension support for non-denaturing micro 2-DE in the separation of high-molecular-mass plasma proteins and protein complexes.Electrophoresis2009;30(6), 939-948.
20.Takashi Manabe,Ya Jin.Noncovalent interactions in human plasma proteins analyzed by the comparison of nondenaturing and denaturing micro-2-D gel electrophoresis patterns after polypeptide assignment using matrix-assisted laser desorption/ionization-mass spectrometry and peptide mass fingerprinting.Electrophoresis2008;29(12),2672-2688.
21.Takashi Manabe,Ya Jin, Nao Yamaguchi, Tetsuo Sugiyama, Kohei Ikari. Cleavage of fibrinogen alpha chains during isoelectric focusing of human plasma under non-denaturing conditions analyzed by micro two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry.Journal of Electrophoresis2007;51(3/4), 27-34.
22.Takashi Manabe,Ya Jin.Analysis of protein/polypeptide interactions in human plasma using nondenaturing micro-2-DE followed by 3-D SDS-PAGE and MS.Electrophoresis2007;28(12), 2065-2079.
23.Takashi Manabe,Ya Jin, Osamu Tani.Assignment of human plasma polypeptides on a nondenaturing 2-D gel using MALDI-MS and PMF and comparisons with the results of intact protein mapping.Electrophoresis2007;28(5), 843-863.
24.Ya Jin, Takashi Manabe.Alkaline extraction of human plasma proteins from nondenaturing micro-2-D gels for protein/polypeptide mass measurement and peptide mass fingerprinting using MALDI-TOF MS.Electrophoresis2007;28(3), 449-459.
25.Maki Kamakura, Keiko Morisawa, Hideyuki Komi, Ayako Tomatani, Fumiji Saito, YukoKonishi,Ya Jin, Takashi Manabe, Masayuki Kuroda, Shosuke Imai, Hiroyuki Mizuguchi, Taketoshi Taniguchi. Regulation of IL-27p28 gene by lipopolysaccharide in dendritic DC2.4 cells.Biochemical and Biophysical Research Communications2006;349(4), 1372-1377.
26.Ya Jin, Takashi Manabe. Direct targeting of human plasma for matrix-assisted laser desorption/ionization and analysis of plasma proteins by time of flight-mass spectrometry.Electrophoresis2005;26(14),2823-2834.
27.Ya Jin, Takashi Manabe.High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry.Electrophoresis2005;26(6), 1019-1028.
28.Takashi Manabe,Ya Jin.Alkaline cleavage of covalent bonds in chicken insulin and bovine-lactalbumin analyzed by matrix-assisted laser desorption/ionization-mass spectrometry.Electrophoresis2005;26(1), 257-267.
29.Ya Jin, Guo-an Luo. Numerical calculation of the electroosmotic flow at the cross region in microfluidic chips.Electrophoresis2003;24(7-8),1242-1252.
30.Ya Jin, Guo-an Luo. Fabrication of the microfluidic chips with integrated ultra-micro electrodes and its application in on-chip electrochemical detection.Chemical Journal of Chinese Universities2003,24(7),1180-1184.Chinese.
31.Ya Jin, Guoan Luo, Tomoko Oka, Takashi Manabe.Estimation of isoelectric points of human plasma proteins employing capillary isoelectric focusing and peptide isoelectric point markers.Electrophoresis2002;23(19), 3385-3391.
32.Ya Jin, Guo-an Luo. Fabrication and applications of an interface to couple microfluidic chips with electrospray ionization mass spectrometer.Analytical Instrumentation (China)2003,(2), 4-10.
33.Guang-hua Zhang, Zhao-ying Zhou, Yang-hua Tang, Guo-an Luo,Ya Jin. The development of micro chip electrophoresis analyser.Chinese Journal of Scientific Instrument2002,(4), 335-338.
34.Guang-hua Zhang, Zhao-ying Zhou, Yang-hua Tang, Guo-an Luo,Ya Jin.Design of abiochemicalsubstanceanalysissystem.Measurement & Control Technology (China)2002,21(4), 10-12.
35.Yang-hua Tang, Xiong-ying Ye, Zhao-ying, Zhou,Ya Jin, Yan-ying Feng.Glass chips for capillary electrophoresis, micromachining, sample injection and separation.Chinese Journal of Analytical Chemistry2001,29(5), 606-610.
36.Ya Jin, Tao Wen, Yi-ming Wang, Guo-an Luo. Development and application of the detection technologies of integrated capillary electrophoresis chips.Modern Scientific Instruments(China)2001,(4), 15-17.
37.Yang-hua Tang, Zhao-ying Zhou, Xiong-ying Ye, Yan-ying Feng,Ya Jin. Microfabrication of capillary electrophoresis chips.Microfabrication Technology(China)2001,(1), 53-55.
38.Ya Jin, Guo-an Luo, Yang-hua Tang, Zhao-ying Zhou. Microfabrication of integrated capillary electrophoresis chips and on-chip sample injection and separation.Journal of Analytical Science(China)2001,17(2), 148-152.
39.Ya Jin, Guo-an Luo.Development of technology and applications of microarrays.Acta Pharmaceutica Sinica(China)2000,35(3), 235-240.
40.Ya Jin, Guo-an Luo, Ru-ji Wang.Development of integrated capillary electrophoresis chips.Se Pu(China)2000,18(4), 313-317.
