鄭何平，男，1979年生，廣東汕頭，博士，湖南大學生物學院教授、嶽麓學者、博士生導師。
基本介紹
- 中文名：鄭何平
- 畢業院校：美國維吉尼亞大學
- 學位/學歷：博士
- 專業方向：計算機技術
- 任職院校：湖南大學
人物經歷,教育經歷,工作經歷,研究領域,科研成果,學術成果,
人物經歷
教育經歷
2001年8月－2009年8月，美國維吉尼亞大學，哲學博士，導師：Wladek Minor教授；
1998年9月－2000年7月，中國科學技術大學計算機技術科學學院，工學學士；
1996年9月－2001年7月，中國科學技術大學生命科學學院，理學學士。
工作經歷
2019年2月至今，湖南大學生物學院，教授，嶽麓學者，博士生導師；
2013年10月－2019年2月，美國維吉尼亞大學，研究科學家，導師：Wladek Minor教授；
2009年8月－2013年10月，美國維吉尼亞大學，博士後研究員，導師：Wladek Minor教授。
研究領域
專業研究手段包括數據挖掘，建模算法，線性回歸，機器學習。
科研成果
在國際學術刊物上發表SCI論文30多篇，包括Nature Protocols，Nucleic Acids Research等知名學術期刊，被引用次數超過1000次，RCR 50.29。主攻生物信息學中，信息學方法模型和算法開發。使用的技術主要包括：
1.數據分析軟體R及其基本操作軟體包，包括但不限於：用於數據操作的dplyr，作圖的ggplot2等；
2.人工智慧機器學習算法軟體包，例如：caret，pandas/scikit-learn，H2O.ai等；
3.集群環境 Proxmox，雲共享軟體開發，軟體容器 Linux container 或者 Docker；
4.關係資料庫 PostgreSQL，分散式資料庫MongoDB；
5.MVC框架如Django，Ruby on Rails等；
6.網站伺服器Nginx和伺服器管理環境node.js；
7.基於bootstrap，web components，React JS或者Angular JS的網頁開發；基於Objective-C或者Java的手機App套用開發。
實驗室的研究方向還包括以X-射線晶體衍射和冷凍電鏡技術為主要手段，結合分子生物學、生物化學、細胞生物學、免疫學、生物物理學等多個學科的方法手段，研究金屬結合蛋白以及金屬藥物與蛋白複合物的結構，闡明其發揮功能的分子機制，並將其套用到診斷治療、培育養殖上面去。例如：（a）鋅依賴性金屬蛋白酶SAS1B：該蛋白在卵細胞和癌細胞表面有特異表達，可以作為癌細胞的天然標記蛋白，所以SAS1B 抗體可作為抗癌藥物的給藥載體。（b）人體血清中轉運小分子的白蛋白（Albumin）：該蛋白具有鋅的特異結合位點，還有可能參與其他金屬小分子的轉運，在金屬結合和轉運中起到重要作用。（c）鈣結合蛋白AVIL：該蛋白的表達與橫紋肌肉瘤密切相關。（d）來自鯉魚的43kD 鋅結合蛋白ZBP：該蛋白作為細胞表面受體參與細胞粘連（cell adhesion），與鯉魚的消化道組織中經常發現極高濃度的鋅有關。
學術成果
1.Handing KB, Niedzialkowska E, Shabalin IG, Kuhn ML, Zheng H*, Minor W* (2018)
Characterizing metal-binding sites in proteins with X-ray crystallography.
Nat Protoc 13: 1062-1090 Times cited: 3 [Pub Med ID: 29674755]
F1000 Recommended Publication
2.Hou J, Zheng H*, Tzou WS, Cooper DR, Chruszcz M, Chordia MD, Kwon K, Grabowski M, Minor W* (2018)
Differences in substrate specificity of V. cholerae FabH enzymes suggest new approaches for the development of novel antibiotics and biofuels.
FEBS J. 285: 2900-2921 [Pub Med ID: 29917313]
3.Porebski PJ, Sroka P, Zheng H, Cooper DR, Minor W (2018)
Molstack-Interactive visualization tool for presentation, interpretation, and validation of macromolecules and electron density maps.
Protein Sci. 27: 86-94 Times cited: 9 [Pub Med ID: 28815771]
4.Zhang J, Zhu Y, Shi Y, Han Y, Liang C, Feng Z, Zheng H, Eng M, Wang J (2017)
Fluoride-Induced Autophagy via the Regulation of Phosphorylation of Mammalian Targets of Rapamycin in Mice Leydig Cells.
J Agric. Food Chem. 65: 8966-8976 Times cited: 6 [Pub Med ID: 28927274]
5.Zheng H, Cooper DR, Porebski PJ, Shabalin IG, Handing KB, Minor W (2017)
CheckMyMetal: a macromolecular metal-binding validation tool.
Acta Crystallogr D Struct Biol 73: 223-233 Times cited: 34 [Pub Med ID: 28291757]
6.Zheng H, Langner KM, Shields GP, Hou J, Kowiel M, Allen FH, Murshudov G, Minor W (2017)
Data mining of iron(II) and iron(III) bond-valence parameters, and their relevance for macromolecular crystallography.
Acta Crystallogr D Struct Biol 73: 316-325 Times cited: 4 [Pub Med ID: 28375143]
7.Zheng H, Porebski PJ, Grabowski M, Cooper DR, Minor W (2017)
Databases, Repositories, and Other Data Resources in Structural Biology.
Methods Mol. Biol. 1607: 643-665 [Pub Med ID: 28573593]
8.Zhang J, Zhang Y, Liang C, Wang N, Zheng H, Wang J (2016)
Choline supplementation alleviates fluoride-induced testicular toxicity by restoring the NGF and MEK expression in mice.
Toxicol Appl Pharmacol. 310: 205-214 Times cited: 2 [Pub Med ID: 27664006]
9.Grabowski M, Langner KM, Cymborowski M, Porebski PJ, Sroka P, Zheng H, Cooper DR, Zimmerman MD, Elsliger MA, Burley SK, Minor W (2016)
A public database of macromolecular diffraction experiments.
Acta Crystallogr D Struct Biol 72: 1181-1193 Times cited: 26 [Pub Med ID: 27841751]
10.Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W (2015)
Dissecting the Structural Elements for the Activation of β-Ketoacyl-(Acyl Carrier Protein) Reductase from Vibrio cholerae.
J. Bacteriol. 198: 463-76 Times cited: 6 [Pub Med ID: 26553852]
11.Zheng H, Mandal A, Shumilin IA, Chordia MD, Panneerdoss S, Herr JC, Minor W (2015)
Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal oolemmal-binding sperm protein, reveals filamentous organization in protein crystal form.
Andrology 3: 756-71 Times cited: 2 [Pub Med ID: 26198801]
12.Zheng H, Shabalin IG, Handing KB, Bujnicki JM, Minor W (2015)
Magnesium-binding architectures in RNA crystal structures: validation, binding preferences, classification and motif detection.
Nucleic Acids Res. 43: 3789-801 Times cited: 32 [Pub Med ID: 25800744]
13.Zheng H, Handing KB, Zimmerman MD, Shabalin IG, Almo SC, Minor W (2015)
X-ray crystallography over the past decade for novel drug discovery - where are we heading next?
Expert Opin Drug Discov 10: 975-89 Times cited: 21 [Pub Med ID: 26177814]
14.Zheng H, Hou J, Zimmerman MD, Wlodawer A, Minor W (2014)
The future of crystallography in drug discovery.
Expert Opin Drug Discov 9: 125-37 Times cited: 20 [Pub Med ID: 24372145]
15.Zheng H, Chordia MD, Cooper DR, Chruszcz M, Müller P, Sheldrick GM, Minor W (2014)
Validation of metal-binding sites in macromolecular structures with the CheckMyMetal web server.
Nat Protoc 9: 156-70 Times cited: 131 [Pub Med ID: 24356774]
16.Domagalski MJ, Zheng H, Zimmerman MD, Dauter Z, Wlodawer A, Minor W (2014)
The quality and validation of structures from structural genomics.
Methods Mol. Biol. 1091: 297-314 Times cited: 9 [Pub Med ID: 24203341]
17.Luo HB, Knapik AA, Petkowski JJ, Demas M, Shumilin IA, Zheng H, Chruszcz M, Minor W (2013)
Biophysical analysis of the putative acetyltransferase SACOL2570 from methicillin-resistant Staphylococcus aureus.
J. Struct. Funct. Genomics 14: 97-108 Times cited: 1 [Pub Med ID: 23963951]
18.Hou J, Wojciechowska K, Zheng H, Chruszcz M, Cooper DR, Cymborowski M, Skarina T, Gordon E, Luo H, Savchenko A, Minor W (2012)
Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 632-7 Times cited: 1 [Pub Med ID: 22684058] [Pub Med Central ID: PMC3370898]
19.Zheng H, Filippova EV, Tkaczuk KL, Dworzynski P, Chruszcz M, Porebski PJ, Wawrzak Z, Onopriyenko O, Kudritska M, Grimshaw S, Savchenko A, Anderson WF, Minor W (2012)
Crystal structures of putative phosphoglycerate kinases from B. anthracis and C. jejuni.
J. Struct. Funct. Genomics 13: 15-26 Times cited: 3 [Pub Med ID: 22403005]
20.Sledz P, Zheng H, Murzyn K, Chruszcz M, Zimmerman MD, Chordia MD, Joachimiak A, Minor W (2010)
New surface contacts formed upon reductive lysine methylation: improving the probability of protein crystallization.
Protein Sci. 19: 1395-404 Times cited: 24 [Pub Med ID: 20506323]
21.Luo HB, Zheng H, Zimmerman MD, Chruszcz M, Skarina T, Egorova O, Savchenko A, Edwards AM, Minor W (2010)
Crystal structure and molecular modeling study of N-carbamoylsarcosine amidase Ta0454 from Thermoplasma acidophilum.
J. Struct. Biol. 169: 304-11 Times cited: 12 [Pub Med ID: 19932181]
22.Chruszcz M, Zimmerman MD, Wang S, Koclega KD, Zheng H, Evdokimova E, Kudritska M, Cymborowski M, Savchenko A, Edwards A, Minor W (2008)
Function-biased choice of additives for optimization of protein crystallization - the case of the putative thioesterase PA5185 from Pseudomonas aeruginosa PAO1.
Cryst Growth Des 8: 4054-4061 Times cited: 3 [Pub Med ID: 19898606]
23.Jha KN, Shumilin IA, Digilio LC, Chertihin O, Zheng H, Schmitz G, Visconti PE, Flickinger CJ, Minor W, Herr JC (2008)
Biochemical and structural characterization of apolipoprotein A-I binding protein, a novel phosphoprotein with a potential role in sperm capacitation.
Endocrinology 149: 2108-20 Times cited: 28 [Pub Med ID: 18202122]
24.Chruszcz M, Potrzebowski W, Zimmerman MD, Grabowski M, Zheng H, Lasota P, Minor W (2008)
Analysis of solvent content and oligomeric states in protein crystals--does symmetry matter?
Protein Sci. 17: 623-32 Times cited: 29 [Pub Med ID: 18359856]
25.Zheng H, Chruszcz M, Lasota P, Lebioda L, Minor W (2008)
Data mining of metal ion environments present in protein structures.
J. Inorg. Biochem. 102: 1765-76 Times cited: 157 [Pub Med ID: 18614239]
