郭國驥，男，現任浙江大學醫學院教授，博士生導師。2014年由浙江大學高層次人才引進計畫從哈佛大學醫學院引進並全職回國，成為浙江大學最年輕的教授之一。2015年入選中組部“國家青年千人計畫”，2017年獲“國家優秀青年基金”。其科研團隊主要利用單細胞分析技術研究幹細胞的再生和分化機制。
基本介紹
- 中文名：郭國驥
- 國籍：中國
- 民族：漢
- 出生地：武漢
- 出生日期：1983年4月12日
- 學位/學歷：博士
- 專業方向：幹細胞與再生醫學
- 職務：教授、博士生導師
個人簡介,工作經歷,研究方向,主要成就,發表文章,
個人簡介
2005年畢業於武漢大學生命科學學院生物技術專業，獲學士學位。2010年畢業於新加坡國立大學，獲得理學博士學位。之後赴美國哈佛大學醫學院從事博士後研究工作，任丹娜法伯癌症研究院及波士頓兒童醫院Research Fellow。2014受聘於浙江大學醫學院，擔任教授至今。2015年入選中組部“國家青年千人計畫”，2017年獲“國家優秀青年基金”。
工作經歷
2001.9-2005.7武漢大學 學士
2005.8-2010.7新加坡國立大學 博士
2010.8-2010.10新加坡基因研究所 博士後
2010.11-2014.9美國哈佛大學醫學院 Research Fellow
2014.10-浙江大學醫學院教授、博士生導師
研究方向
單細胞分析技術
主要成就
利用單細胞基因表達譜分析的手段來研究幹細胞的分化路徑和分化機制，開闢了單細胞系統生物學新領域。建立並最佳化了高通量單細胞定量PCR的基本實驗方法，以及數據分析手段。構建Microwell-seq高通量單細胞分析平台，並繪製全球首個哺乳動物細胞圖譜。在胚胎期幹細胞系統和成體造血幹細胞系統中發現了譜系基因預表達的現象，解析了這一現象的分子機制，並進一步闡明了其對幹細胞終端分化的影響。五年內在Cell, Cell Stem Cell, Developmental Cell等著名期刊發表學術論文10餘篇，總影響因子180分，總引用率650餘次。
發表文章
Han X, Chen H, Huang D, Chen H, Fei L, Cheng C, Huang H, Yuan GC, Guo G. Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing. Genome Biol. 2018 Apr 5;19(1):47.
Han X, Wang R, Zhou Y, Fei L, Sun H, Lai S, Saadatpour A, Zhou Z, Chen H, Ye F, Huang D, Xu Y, Huang W, Jiang M, Jiang X, Mao J, Chen Y, Lu C, Xie J, Fang Q, Wang Y, Yue R, Li T, Huang H, Orkin SH, Yuan GC, Chen M, Guo G. Mapping the Mouse Cell Atlas by Microwell-Seq.Cell. 2018 Feb 22; 172(5):1091-1107.e17.
Han X, Yu H, Huang D, Xu Y, Saadatpour A, Li X, Wang L, Yu J, Pinello L, Lai S, Jiang M, Tian X, Zhang F, Cen Y, Fujiwara Y, Zhu W, Zhou B, Zhou T, Ouyang H, Wang J, Yuan GC, Duan S, Orkin SH, Guo G. A molecular roadmap for induced multi-lineage trans-differentiation of fibroblasts by chemical combinations. Cell Res. 2017 Mar;27(3):386-401.
Beyaz S, Mana MD, Roper J, Kedrin D, Saadatpour A, Hong SJ, Bauer-Rowe KE, Xifaras ME, Akkad A, Arias E, Pinello L, Katz Y, Shinagare S, Abu-Remaileh M, Mihaylova MM, Lamming DW, Dogum R, Guo G, Bell GW, Selig M, Nielsen GP, Gupta N, Ferrone CR, Deshpande V, Yuan GC, Orkin SH, Sabatini DM, Yilmaz ÖH. High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature. 2016 Mar 3;531(7592):53-8.
Guo G#，Pinello L, Han X, Lai S, Shen L, Lin TW, Zou K, Yuan GC#, Orkin SH#. Serum-based culture conditions provoke gene expression variability in mouse embryonic stem cells as revealed by single cell analysis. Cell Rep. 2016 Jan 19;14(4):956-65. #Co-corresponding authors.
Saadatpour A, Lai S, Guo G#, Yuan GC#. Single-Cell Analysis in Cancer Genomics. Trends Genet. 2015 Oct;31(10):576-86. doi: 10.1016/j.tig.2015.07.003. #Co-corresponding authors.
Saadatpour A*, Guo G*, Orkin SH, Yuan GC. Characterizing heterogeneity in leukemic cells using single-cell gene expression analysis. Genome Biol. Genome Biol. 2014 Dec 3;15(12):525. doi: 10.1186/s13059-014-0525-9. *Co-first authors.
Graham SL, Wicher KB, Jedrusik A, Guo G, Herath W, Robson P and Zernicka-Goetz M. BMP signalling regulates the pre-implantation development of extra-embryonic cell lineages in the mouse embryo. Nat Commun. 2014 Dec 16;5:5667. doi: 10.1038/ncomms6667.
Marco E, Karp RL, Guo G, Robson P, Hart AH, Trippa L, Yuan GC. Bifurcation analysis of single-cell gene expression data reveals epigenetic landscape. Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):E5643-50. doi: 10.1073/pnas.1408993111. Epub 2014 Dec 15.
Lundberg P, Takizawa H, Kubovcakova L, Guo G, Hao-Shen H, Dirnhofer S, Orkin SH, Manz MG, Skoda RC. Myeloproliferative neoplasms can be initiated from a single hematopoietic stem cell expressing JAK2-V617F. J Exp Med. 2014 Oct 20;211(11):2213-30. doi: 10.1084/jem.20131371. Epub 2014 Oct 6.
Riddell J, Gazit R, Garrison BS, Guo G, Saadatpour A, Mandal PK, Ebina W, Volchkov P, Yuan GC, Orkin SH, Rossi DJ. Reprogramming committed murine blood cells to induced hematopoietic stem cells with defined factors. Cell. 2014 Apr 24;157(3):549-64. doi: 10.1016/j.cell.2014.04.006.
Das PP, Shao Z, Beyaz S, Apostolou E, Pinello L, De Los Angeles A, O'Brien K, Atsma JM, Fujiwara Y, Nguyen M, Ljuboja D, Guo G, Woo A, Yuan GC, Onder T, Daley G, Hochedlinger K, Kim J, Orkin SH. Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity. Mol Cell. 2014 Jan 9;53(1):32-48. doi: 10.1016/j.molcel.2013.11.011. Epub 2013 Dec 19.
Guo G, Luc S, Marco E, Lin TW, Peng C, Kerenyi MA, Beyaz S, Kim W, Xu J, Das PP, Neff T, Zou K, Yuan GC, Orkin SH. Mapping cellular hierarchy by single-cell analysis of the cell surface repertoire. Cell Stem Cell. 2013 Oct 3;13(4):492-505. doi: 10.1016/j.stem.2013.07.017. Epub 2013 Sep 12.
Kim W, Bird GH, Neff T, Guo G, Kerenyi MA, Walensky LD, Orkin SH. Targeted disruption of the EZH2-EED complex inhibits EZH2-dependent cancer. Nat Chem Biol. 2013 Oct;9(10):643-50. doi: 10.1038/nchembio.1331. Epub 2013 Aug 25.
Kerenyi MA, Shao Z, Hsu YJ, Guo G, Luc S, O'Brien K, Fujiwara Y, Peng C, Nguyen M, Orkin SH. Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation. Elife. 2013 Jun 18;2:e00633. doi: 10.7554/eLife.00633.
Do DV, Ueda J, Messerschmidt DM, Lorthongpanich C, Zhou Y, Feng B, Guo G, Lin PJ, Hossain MZ, Zhang W, Moh A, Wu Q, Robson P, Ng HH, Poellinger L, Knowles BB, Solter D, Fu XY. A genetic and developmental pathway from STAT3 to the OCT4-NANOG circuit is essential for maintenance of ICM lineages in vivo. Genes Dev. 2013 Jun 15;27(12):1378-90. doi: 10.1101/gad.221176.113.
Maclean GA, Menne TF, Guo G, Sanchez DJ, Park IH, Daley GQ, Orkin SH. Altered hematopoiesis in trisomy 21 as revealed through in vitro differentiation of isogenic human pluripotent cells. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17567-72. doi: 10.1073/pnas.1215468109. Epub 2012 Oct 8.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P. Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell. 2010 Apr 20;18(4):675-85. doi:10.1016/j.devcel.2010.02.012.
Ralston A, Cox BJ, Nishioka N, Sasaki H, Chea E, Rugg-Gunn P, Guo G, Robson P, Draper JS, Rossant J. Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2. Development. 2010 Feb;137(3):395-403. doi:10.1242/dev.038828.
Yuan P, Han J, Guo G, Orlov YL, Huss M, Loh YH, Yaw LP, Robson P, Lim B, Ng HH. Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells. Genes Dev. 2009 Nov 1;23(21):2507-20. doi: 10.1101/gad.1831909.
Jedrusik A, Parfitt DE, Guo G, Skamagki M, Grabarek JB, Johnson MH, Robson P, Zernicka-Goetz M. Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo. Genes Dev. 2008 Oct 1;22(19):2692-706. doi: 10.1101/gad.486108.
