基本介紹
- 中文名：連正興
- 畢業院校：東北農業大學
- 學位/學歷：博士
- 專業方向：動物分子與細胞工程育種
- 任職院校：中國農業大學
人物經歷,教育經歷,工作經歷,社會兼職,研究方向,教學工作,學術成果,科研工作,發表論文,獲獎記錄,
人物經歷
教育經歷
1992/09-1996/06：東北農業大學，動物科技學院動物遺傳育種與繁殖系，博士
1986/09-1989/06：東北農學院畜牧系，碩士
1982/09-1986/06：東北農學院畜牧系，學士
工作經歷
2003/07-至今：中國農業大學，動物科技學院，動物遺傳育種與繁殖系，教授
1996/07-2003/07：中國農業大學，動物科技學院，動物遺傳育種與繁殖系，副教授
1989/07-1992/07：東北農業大學，動物科技學院，動物遺傳育種與繁殖系，講師
2000/01-2000/04：法國農業科學院（INRA）合作研究
2008/01-2008/12：英國Institute of Animal Health in UK，訪問學者
社會兼職
中國農業生物技術學會，副秘書長
中國農業生物技術學會動物生物技術分會，秘書長
中國畜牧獸醫學會動物遺傳育種學分會，理事
全國農業轉基因生物安全管理標準化技術委員會，委員
研究方向
動物分子與細胞工程育種：主要致力於羊雞豬分子與細胞學標記研發與產業化推廣；完善與發展畜禽轉基因和基因編輯技術，深度挖掘與畜禽抗病能力、肌肉和毛囊發育相關基因或標記，闡明重要經濟性狀發生與發展的分子機制。
教學工作
《動物細胞遺傳學》，本科選修
《羊的育種》，本科選修
《動物分子遺傳學》，研究生必修
《動物大分子研究實驗技術》，研究生必修
學術成果
科研工作
主持了國家轉基因生物新品種培育重大課題：抗病轉基因羊新品種培育課題，利用轉基因技術，瞄準人畜共患的布魯氏桿菌（誘發布氏桿菌病，布病），通過過表達先天免疫分子TLR4基因，提高轉基因羊對布氏桿菌的探測能力，進而防止布氏桿菌的免疫逃逸，無論在布病爆發疫區還是活體攻毒，轉基因羊均表現出明顯的抗布病能力，為牧民的身心健康和畜產品的安全性均提供了全新解決方案。在此基礎上，通過CRISPR/CAS9基因編輯技術，在國際上率先獲得了MSTN和FGF5基因編輯綿羊，掌握了國際前沿技術。利用自主開發的規模化雞外周血單核/巨噬細胞吞噬能力檢測試劑盒，雙向、多代選擇高低吞噬積群體，低吞噬積群體的產蛋率明顯優於高吞噬積群體，為提高蛋雞產蛋率提供了新型細胞學標記。
目前在研項目
1. 抗病轉基因羊新品種培育，2016/01-2020/12、主持。
3. 抗病轉基因羊新品種培育，2011/01-2015/12、主持。
發表論文
1. Yu D, Wang J, Zou H, Feng T, Chen L, Li J, Qi X, Li Z, Duan X, Xu C, Zhang L, Long X, Lan J, Chen C, Wang C, Xu X, Ren J, Zhao Y, Hu X, Lian Z, Men H, Pan D, Li N, Capecchi MR, Du X, Zhao Y, Wu S. Silencing of retrotransposon-derived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning. PNAS. 2018
2. Shoulong Deng et al. Melatonin promotes sheep Leydig cell testosterone secretion in a co-culture with Sertoli cells. Theriogenology. 2018
3. Jingling Tao et al. Effects of AANAT overexpression on the inflammatory responses and autophagy activity in the cellular and transgenic animal levels Autophagy. 2018
4. Sutian Wang et al. Overexpression of Toll-like Receptor 4-linked Mitogen-activated Protein Kinase Signaling Contributes to Internalization of Escherichia coli in Sheep. International Journal of Biological Sciences. 2018
5. Yitong Yuan et al. Dwarf chickens with low monocytesmacrophages phagocytic activity show low antibody titers but greater performance. Animal Reproduction Science. 2018
6. Yitong Yuan et al. Interferon-γ acts as a regulator in the trade-off between phagocytosis and production performance in dwarf chickens. Journal of Animal Science and Biotechnology . 2018
7. Li Zhang et al. An Alternative Method for Long-Term Culture of Chicken Embryonic Stem Cell In Vitro. Stem Cells International. 2018
8. Shoulong Deng et al. Erratum to: Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats Journal. of Animal Science and Biotechnology. 2017
9. Shoulong Deng et al. In vitro production of functional haploid sperm cells from male germ cells of Saanen dairy goat. Theriogenology. 2017
10. Shoulong Deng et al. Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats. Journal of Animal Science and Biotechnology. 2017
11. Shoulong Deng et al. RNAi combining Sleeping Beauty transposon system inhibits ex vivo expression of foot-and-mouth disease virus VP1 in transgenic sheep cells. Science Report. 2017
12. Teng Ma et al. An AANAT/ASMT transgenic animal model constructed with CRISPRCas9 system serving as the mammary gland bioreactor to produce melatonin-enriched milk in sheep. Journal of Pineal Research. 2017
13. Xiuzhi Tian et al. Beneficial Effects of Melatonin on the In Vitro Maturation of Sheep Oocytes and Its Relation to Melatonin Receptors. International Journal of Molecular Sciences. 2017
14. Xiuzhi Tian et al. Melatonin Promotes the In Vitro Development of Microinjected Pronuclear Mouse Embryos via Its Anti-Oxidative and Anti-Apoptotic Effects. International Journal of Molecular Sciences. 2017
15. Hongping Wu et al. The evolutionary characteristics and structural biology of Gallus toll‐like receptor 21 Journal of Molecular Recognition 2017
16. Mingming Wu et al. Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system. Science Report. 2016
17. Yan Li et al. Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4. Journal of Animal Science and Biotechnology. 2016
18. Hai Wang et al. Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacterium in the intestine and improves postnatal growth of chicken. AMB Express. 2016
19. Kun Yu et al. Small interfering RNA expression inhibits avian infectious bronchitis virus replication and inflammatory response Antiviral Therapy 2016
20. Shi JM Effects of seasonal changes on the ovulation rate and embryo quality in superovulated Black Suffolk ewes. Neuro endocrinology letters. 2015
21. Yuanhong Xie et al. Hypocholesterolemic effects of Kluyveromyces marxianus M3 isolated from Tibetan mushrooms on diet-induced hypercholesterolemia in rat. Brazilian journal of microbiology. 2015
22. Zhang Y et al. Endothelial Cells Regulate Cardiac Myocyte Reorganisation Through β1-Integrin Signalling. Cellular Physiology and Biochemistry. 2015
23. Hai Bai et al. A 90-day toxicology study of meat from genetically modified sheep overexpressing TLR4 in Sprague-Dawley rats. Plos One. 2015
24. Shoulong Deng et al. Melatonin reduces oxidative damage and upregulates heat shock protein 90 expression in cryopreserved human semen. Free Radical Biology and Medicine. 2015
25. Shoulong Deng et al. Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep Monocytes/Macrophages. Oxidative Medicine and Cellular Longevity. 2015
26. Shoulong Deng et al. Toll-Like Receptor 4 Reduces Oxidative Injury via Glutathione Activity in Sheep Oxidative Medicine and Cellular Longevity 2015
27. Wenting Li et al. Tongue Epithelium Cells from shRNA Mediated Transgenic Goat Show High Resistance to Foot and Mouth Disease Virus. Science Report. 2015
28. Zhang L et al. Effects of melatonin on superovulation and transgenic embryo transplantation in small-tailed han sheep (Ovis aries). Neuro endocrinology letters. 2013
29. Shoulong Deng et al. Transgenic cloned sheep overexpressing ovine toll-like receptor 4. Theriogenology.2013
30. Miaoying Yu et al. Four recombinant pluripotency transcriptional factors containing a protein transduction domain maintained the in vitro pluripotency of chicken embryonic stem cells. Science China Life Sciences.2013
