葉明亮，中國科學院特聘研究員，國家自然科學基金委傑出青年基金獲得者。2001 年在中科院大連化物所獲博士學位，同年底赴美國華盛頓大學化學系進行博士後研究，2003 年轉美國系統生物研究所從事蛋白質組學新方法研究，師從著名蛋白質組學專家Ruedi Aebersold教授。2004年回國併入選中科院百人計畫，2015年獲得傑出青年基金資助，2016年入選中國科學院特聘研究員特聘骨幹人才和科技部中青年科技創新領軍人才。為中國生物化學與分子生物學會蛋白質組學專業委員會常委。
基本介紹
- 中文名：葉明亮
- 國籍：中國
- 職業：研究員
- 畢業院校：中科院
- 性別：男
概述,代表性文章,
概述
葉明亮 研究員 博士生導師 中科院大連化學物理研究所 生物技術部
葉明亮，以色譜分離與質譜分析為立足點，針對蛋白質組等複雜生物樣品的高效分離與高靈敏檢測，開展蛋白質組分離分析新技術和新方法的研究，注重分析化學與生物學、數學、生物信息學等學科的交叉。在蛋白質翻譯後修飾的高靈敏、規模化分析方面建立了多項原創技術，在Nat. Methods , Nat. Chem. Biol., Nat. Protoc., Angew. Chem. Int. Ed. 等SCI期刊上發表責任作者（第一作者和通訊作者）論文70餘篇，他引2000餘次（所有SCI論文170餘篇，他引4400餘次）。曾獲中科院院長獎學金特別獎（2001年），遼寧省自然科學獎兩次（2004年排名第4；2011年排名第3；），國家自然科學二等獎一次(2012年排名第3)。先後主持基金委傑出青年基金、國家重點研發計畫重點專項、基金委中加健康合作項目、科技部創新方法項目、國家重大科學研究計畫課題等項目。
葉明亮博士主要研究方向為蛋白質組新技術新方法的研究，主要包括：
l 複雜生物樣品預處理技術和方法
基於納米材料的富集技術和方法、蛋白質組樣品預處理的集成化技術、同位素標記技術
l 高效分離柱技術與多維分離分析系統
高效整體分離柱技術；多維分離分析方法；質譜數據處理方法
l 蛋白質組修飾譜規模化分析技術和方法
蛋白質磷酸化、糖基化等翻譯後修飾的分離、規模化鑑定新方法及其套用
l 套用研究
血液淨化材料、生物分離材料、東北特色資源研究與開發
代表性文章
REPRESENTATIVE PUBLICATIONS
1）Yangyang Bian, Lei Li, Mingming Dong, Xuguang Liu, Tomonori Kaneko, Kai Cheng, Huadong Liu, Courtney Voss, Xuan Cao, Yan Wang, David Litchfield,Mingliang Ye*, Shawn S.-C. Li*, and Hanfa Zou*. Ultra-deep tyrosine phosphoproteomics enabled by a phosphotyrosine superbinder,Nature Chemical Biology, 2016, in press.
2）Ye, Mingliang*;Pan, Yanbo; Cheng, Kai; Zou, Hanfa*. Protein Digestion Priority is Independent of Protein Abundances.Nature Methods, 2014, 11: 220-222.
3）Pan, Yanbo;Ye, Mingliang*;Zhao, Liang; Cheng, Kai; Dong, Mingming; Song, Chunxia; Qin,Hongqiang; Wang, Fangjun; Zou, Hanfa*. N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics.Angewandte Chemie International Edition, 2013, 52(35): 9205-9209.
4)Houjiang Zhou#;Mingliang Ye#; Jing Dong; Eleonora Corradini; Alba Cristobal; Albert J R Heck*; Hanfa Zou*; Shabaz Mohammed*.Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography.Nature Protocols, 2013, 8,3,461-480
5）ZhenzhenDeng; Jiawei Mao; Yan Wang; HanfaZou; Mingliang Ye*.Enzyme kinetics for complex system enables accurate determination of specificity constants of numerous substrates in a mixture by proteomics platform,Molecular & Cellular Proteomics, 2016,Papersin Press
6）Zhang, Zhang; Sun, Zhen; Zhu, Jun; Liu, Jing; Huang, Guang;Ye, Mingliang*;Zou, Hanfa*. High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis.Analytical Chemistry, 2014, 86(19): 9830-9837.
7）Liu, Fangjie;Ye, Mingliang*;Pan, Yanbo; Zhang, Yi; Bian, Yangyang; Sun, Zhen; Zhu, Jun; Cheng, Kai; Zou, Hanfa*. Integration of Cell Lysis, Protein Extraction, and Digestion into One Step for Ultrafast Sample Preparation for Phosphoproteome Analysis.Analytical Chemistry,2014, 86(14): 6786-6791.
8）Pan, Yanbo;Ye, Mingliang*;Zheng, Hao; Cheng, Kai; Sun, Zhen; Liu, Fangjie; Liu, Jing; Wang, Keyun; Qin, Hongqiang; Zou, Hanfa*. Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis. Analytical Chemistry, 2014, 86(2): 1170-1177.
9）Junfeng Huang, Hongqiang Qin, Zhen Sun, Guang Huang, Jiawei Mao, Kai Cheng, Zhang Zhang, Hao Wan, Yating Yao, Jing Dong, Jun Zhu, Fangjun Wang,Mingliang Ye*, Hanfa Zou. A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method.Scientific Reports, 2015, 5:10164, 1-15
7）Liu, Fangjie;Ye, Mingliang*;Pan, Yanbo; Zhang, Yi; Bian, Yangyang; Sun, Zhen; Zhu, Jun; Cheng, Kai; Zou, Hanfa*. Integration of Cell Lysis, Protein Extraction, and Digestion into One Step for Ultrafast Sample Preparation for Phosphoproteome Analysis.Analytical Chemistry,2014, 86(14): 6786-6791.
8）Pan, Yanbo;Ye, Mingliang*;Zheng, Hao; Cheng, Kai; Sun, Zhen; Liu, Fangjie; Liu, Jing; Wang, Keyun; Qin, Hongqiang; Zou, Hanfa*. Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis. Analytical Chemistry, 2014, 86(2): 1170-1177.
9）Junfeng Huang, Hongqiang Qin, Zhen Sun, Guang Huang, Jiawei Mao, Kai Cheng, Zhang Zhang, Hao Wan, Yating Yao, Jing Dong, Jun Zhu, Fangjun Wang,Mingliang Ye*, Hanfa Zou. A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method.Scientific Reports, 2015, 5:10164, 1-15
10）Wang, Chunli;Ye, Mingliang*;Bian, Yangyang; Liu, Fangjie; Cheng, Kai; Dong, Mingming; Dong, Jing; Zou, Hanfa. Determination of CK2 Specificity and Substrates by Proteome-Derived Peptide Libraries.Journal of Proteome Research, 2013, 12(8): 3813-3821.
11）Deng, Zhenzhen，Ye, Mingliang*，Bian, Yangyang，Liu, Zheyi，Liu, Fangjie，Wang, Chunli; Qin, Hongqiang，Zou, Hanfa*，Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS,Chemical Communications, 2014, 50(90): 13960-13962.
12）FangjieLiu,HaoWan,ZhongshanLiu,HongweiWang,JiaweiMao,MingliangYe*,HanfaZou*. Preparation of Polypropylene Spin Tips Filled with ImmobilizedTitanium(IV) Ion Monolithic Adsorbent for RobustPhosphoproteomeAnalysis.Analytical Chemistry, 2016, 88, 5058-5064
