研究方向
基於結構生物學、分子生物學、蛋白質工程手段研究碳水化合物代謝酶類的分子結構及催化機理,探索新型食品酶的高效發掘與分子改造手段。
(2)食品生物防腐保鮮技術
探索基於植物免疫誘抗、生防微生物、酶製劑的果蔬綠色防腐與安全保鮮技術。
(3)食品功能因子挖掘與綠色生物製造
益生元、功能糖等新型食品功能因子的挖掘、活性探索及酶法定向製備。
主持科研項目
(1)國家自然科學基金青年基金,31701537,新型殼聚糖酶結構-功能解析及其理性改造研究,主持
(2)上海市青年科技英才“揚帆計畫”,17YF1403500,殼聚糖酶產物聚合度差異的分子機制研究,主持
(3)上海市“晨光計畫”,17CG28,碳水化合物結合模組的作用機制及其在功能低聚糖製備中的套用研究,主持
(4)中央高校基本科研業務費,222201814031,基於CBM56結構域融合策略固定化糖苷水解酶製備功能寡糖,主持
代表論文
1.Luo, S., Qin, Z.*, Chen, Q.,Fan, L., Jiang, L. & Zhao, L.* Highlevel production of a Bacillusamlyoliquefaciens chitosanase in Pichiapastoris suitable for chitooligosaccharides preparation, International Journal of BiologicalMacromolecules 2020, 149:1034–1041. (JCR Q1, IF 5.162)
2.Qin, Z., Lin, S.,Qiu, Y., Chen, Q., Zhang, Y., Zhou, J. & Zhao, L. One-step immobilization-purification of enzymes bycarbohydrate-binding module family 56 tag fusion, Food Chemistry 2019,299: 125037. (JCR Q1, IF 6.306)
3.Lin, S., Qin, Z., Chen, Q., Fan, L.,Zhou, J., & Zhao, L. Efficientimmobilization of bacterial GH family 46 chitosanase by carbohydrate-bindingmodule fusion for the controllable preparation of chitooligosaccharides. Journal of Agricultural and Food Chemistry 2019, 67: 6847–6855. (JCR Q1, IF 4.192)
4. Qin, Z.;Chen, Q.; Lin, S.; Luo, S.; Qiu, Y.; Zhao, L., Expression andcharacterization of a novel cold-adapted chitosanase suitable forchitooligosaccharides controllable preparation. Food Chemistry 2018,253: 139–147. (JCR Q1, IF 6.306)
5. Qin, Z.; Luo, S.; Li, Y.; Chen, Q.; Qiu, Y.; Zhao, L.;Jiang, L.; Zhou, J., Biochemical properties of a novel chitosanase from Bacillus amyloliquefaciens and its usein membranereactor. LWT-Food Science and Technology 2018, 97: 9–16.(JCR Q1, IF 4.006)
6.Qin, Z.; Yang,S.; Zhao, L.; You, X.; Yan, Q.; Jiang, Z., Catalyticmechanism of a novel glycoside hydrolase family 16“elongating” β-transglycosylase. Journal of Biological Chemistry 2017,292: 1666–1678. (JCR Q2, IF4.238)
7.Qin, Z.; Yang,D.; You, X.; Liu, Y.; Hu, S.; Yan, Q.; Yang, S.; Jiang, Z.,Therecognition mechanism of triple-helicalβ-1,3-glucanby a β-1,3-glucanase. Chemical Communications 2017, 53:9368–9371. (JCR Q1, IF 5.99)
8.Qin, Z., Yan, Q., Yang, S.,Jiang, Z., Modulating the function of a β-1,3-glucanosyltransferaseto that of an endo-β-1,3-glucanaseby structure-based protein engineering. AppliedMicrobiology and Biotechnology 2016, 100: 1765–1776.(JCR Q2, IF 3.67)
9.Qin, Z.; Xiao, Y.; Yang, X.;Mesters, J. R.; Yang, S.; Jiang, Z., A unique GCN5-relatedglucosamine N-acetyltransferaseregion exist in the fungal multi-domain glycoside hydrolase family3 β-N-acetylglucosaminidase. Scientific Reports 2016, 5: 18292.(JCR Q1, IF 3.998)
10.Qin, Z., Yan, Q., Lei, J., Yang, S., Jiang, Z., &Wu, S. The first crystal structure of a glycoside hydrolase family 17 beta-1,3-glucanosyltransferase displays a unique catalytic cleft. ActaCrystallographica Section D-Structural Biology 2015, 71: 1714-1724.(JCR Q1, IF 5.266)
專利
1. 一種耐熱N-乙醯氨基葡萄糖脫乙醯酶及其編碼基因與套用. CN110484525A
2. 一種脫乙醯酶及其編碼基因與套用. CN110511917A
3. 碳水化合物結合模組及其在固定化酶製備與融合蛋白純化上的套用. CN109266633A
4. 一種重組殼聚糖酶及其生產方法與套用. CN108611337A
5. 一種低溫殼聚糖酶及其編碼基因與套用. ZL201710177429.1