淡橙黃色甲烷氧化桿菌

編號
AMS-86-17
拉丁菌名
Renoides gen. nov.
中文菌名
淡橙黃色甲烷氧化桿菌
定名人
梁家原 程光勝 陳子英
Nomencla
Zhou Huiling, Xiao Changsong, Zhou Yuguang, and Wang Dasi
發表文章
自沼氣池中分離的一株甲烷利用菌
Article
A New Bacterial Genus --- Renoides gen. nov.
作者
梁家原 程光勝 陳子英
Author
Zhou Huiling, Xiao Changsong, Zhou Yuguang, and Wang Dasi
作者單位
中國科學院 微生物研究所細菌分類學實驗室沼氣組, 北京 100080
摘要
自沼氣池中分離到一株甲烷利用 菌, 其主要生理特性是利用甲烷或甲醇作碳源和能源, 生 活史中具有休眠體, 形成固氮菌型的孢囊, 在利用甲烷的 生長中不被乙酸鹽,蘋果酸, 琥珀酸鹽抑制, 應是甲基桿 菌屬 (Methylobacter) 中的一新種, 定名為淡橙黃色甲 烷氧化桿菌 (Methylobacter luteolo-croceus sp. nov.).
Abstract
A strain of Gram negative curved bacterium, No.9, was isolated from the inside of root of Spartina anglica grown on the sea shore of Rutung County,Jiangsu Province. The cells of bacterium. No. 9 are kidney shaped mostly, some are vibroid in shape. Cell diameter is 0.5-0.7 micrometer,the length of the outside curve, 1.1-2.5 micrometer. There are small spines at the surface of the cells. No gas vacuoles exists in cellsbut B-hydroxybutyric acid granules. Non-motile. Chemoorganothroph and facultative chemolithotroph, CO2 as carbon source and H2 as energy source.Acetic acid, butyric acid, succinic acid, malic acid, citric acid, fumaric acid, 2,3-butylene glycol, proline, phenylalanine, casein and betaine are usedas carbon sources. Formic acid, methanol, propoinic acid, iso-butyric acid, valeric acid, iso-valeric acid, caproic acid, malonic acid, methanol,glycine, arginine, or hippuric acid does not be used as carbon source. Metabolism respiratory, nover fermentative. In media without combined nirrogen,microaerophilic growth will occur and molecular nitrogen will be fixed. Catalase and oxidase positive. Acid is produced from xylose, arabinose,glucose, fructose, galactose, mannose, lactose, rhamnose, sorbitol and 2, 3-butylene glycol. No acid is produced from ribose, maltose, sucrose,melibiose, cellubiose, trehalose, melizitose, raffinose, inuline, starch, dextrin, glycogen, ethanol, erythritol, glycerol, adonitol, mannitol,sorbitol, dulcitol, salicin and inositol. Growth occurs in broth containing 0-4.5% NaCl. Temperature range for growth is 10-41'C, optimum,30-35'C Growth pH range is 6.0-9.5, optimum, 7.5-8.0. GC content in DNA is 68.4 mol %. According to Bergey's Manual of Systematic Bacteriology(1984), bacterium No.9 belongs to the group of non-motile Gram negative curved bacteria. Although it is quite similar to Microcyclus. there aresome differences obviously as above memtioned. We propose a new Genus name, Renoides gen. nov., and a new species name, Renoides spartin acae sp. nov.,for bacterium No.9. The culture is deposited in Type Culture Collection, Institute of Microbiology, Academic Sinica, with a catalogue number AS 1.1761.
關鍵字
甲基桿 菌屬, 淡橙黃色甲烷氧化桿菌
Key
期刊
微生物學報 27(1):6-9, 1987.
Publication
Acta Microbiologica Sinica (Chinese; English Abstract), 26(4):pp.289-294, 1986.
分享省市
Place
Rutung County, Jiangsu Province, China
採集地
沼氣池污泥
Environment
The bacterium isolated from the inside of root of the Yellow Sea seabeach at Spartina anglica grown on the sea shore
海撥高度
生態環境
沼氣池
寄主
沼氣池污泥
Habitat
subtropical moist monsoon climate grass seabeach
Host
the inside of root of Spartina anglica
採集人
梁家原 程光勝 陳子英
Isolation Person
Zhou Huiling
採集時間
保存單位
中國科學院微生物研究所細菌分類學實驗室沼氣組, 北京 100080
Preservation Unit
Type Culture Collection, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China
描述
等模式
Speciman No.
No.9
全文
Note
Spartina anglica grown on the sea shore or saline-alcali soil, it is famous fogage garss for animals.The grass as well is a important plant for transform saline-alcali land and establish new agricultural ecological environment.

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