《梨果實糖酸性狀形成的分子機制及重要功能基因的挖掘》是張紹鈴為項目負責人，南京農業大學為依託單位的青年科學基金項目。
基本介紹
- 中文名：梨果實糖酸性狀形成的分子機制及重要功能基因的挖掘
- 項目類別 ：重點項目
- 依託單位 ：南京農業大學
- 項目負責人：張紹鈴
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri, enhances sucrose content in Solanum lycopersicum fruit | 期刊論文 | Wang, Li-Fen(#); Qi, Xiao-Xiao; Huang, Xiao-San; Xu, Lin-Lin; Jin, Cong; Wu, Jun; Zhang, Shao-Ling(*) |
2 | An AFLP, SRAP, and SSR Genetic Linkage Map and Identification of QTLs for Fruit Traits in Pear (Pyrus L.) | 期刊論文 | Zhang, Rui-ping(#); Wu, Jun(*); Li, Xiu-gen; Khan, M. Awais; Chen, Hui; Korban, Schuyler S.; Zhang, Shao-ling(*) |
3 | Evolution of the Aroma Volatiles of Pear Fruits Supplemented with Fatty Acid Metabolic Precursors | 期刊論文 | Qin, Gaihua(#); Tao, Shutian; Zhang, Huping; Huang, Wenjiang; Wu, Juyou; Xu, Yiliu; Zhang, Shaoling(*) |
4 | Molecular characterization and expression pattern of sorbitol transporter gene PbSOT2 in Pear (Pyrus bretschneideri Rehd.) fruit | 期刊論文 | Wang, Lifen(#); Qi, Xiaoxiao; Yang, Yanan; Zhang, Shaoling(*) |
5 | Construction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.) | 期刊論文 | Chen, Hui(#); Song, Yue; Li, Lei-Ting; Khan, M. Awais; Li, Xiu-Gen; Korban, Schuyler S.; Wu, Jun(*); Zhang, Shao-Ling(*) |
6 | Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.) | 期刊論文 | Wei, Shuwei(#); Tao, Shutian; Qin, Gaihua; Wang, Shaomin; Tao, Jihan; Wu, Jun; Wu, Juyou; Zhang, Shaoling(*) |
7 | Apoplastic calmodulin promotes self-incompatibility pollen tube growth by enhancing calcium influx and reactive oxygen species concentration in Pyrus pyrifolia | 期刊論文 | Jiang, Xueting(#); Gao, Yongbin; Zhou, Hongsheng; Chen, Jianqing; Wu, Juyou; Zhang, Shaoling(*) |
8 | Identification and testing of reference genes for gene expression analysis in pollen of Pyrus bretschneideri | 期刊論文 | Chen, Jianqing(#); Li, Xinyue; Wang, Danqi; Li, Leiting; Zhou, Hongsheng; Liu, Zhe; Wu, Jun; Wang, Peng; Jiang, Xueting; Fabrice, Musana R.; Zhang, Shaoling(*); Wu, Juyou(*) |
9 | Comparative Genomic Analysis Reveals Multiple Long Terminal Repeats, Lineage-Specific Amplification, and Frequent Interelement Recombination for Cassandra Retrotransposon in Pear (Pyrus bretschneideri Rehd.) | 期刊論文 | Yin, Hao(#); Du, Jianchang; Li, Leiting; Jin, Cong; Fan, Lian; Li, Meng; Wu, Jun; Zhang, Shaoling(*) |
10 | Long-chain base phosphates modulate pollen tube growth via channel-mediated influx of calcium | 期刊論文 | Wu, Juyou(#); Qin, Xiaoya; Tao, Shutian; Jiang, Xueting; Liang, Yun-Kuan; Zhang, Shaoling(*) |
11 | PbCOL8 is a clock-regulated flowering time repressor in pear | 期刊論文 | Wang, Peng(#); Liu, Zhe(#); Cao, Peng; Liu, Xueying; Wu, Xiaoping; Qi, Kaijie; Zhang, Shaoling(*); Wu, Juyou(*) |
12 | 一種提取梨果實蔗糖轉化酶及其活性測定方法 | 專利 | 吳俊; 楊志軍; 張紹鈴; 齊開傑; 陶書田; 吳巨友 |
13 | 梨果實品質性狀優異基因發掘與分子育種技術研究 | 科研獎勵 | 吳俊; 張紹鈴; 滕元文; 張虎平; 吳巨友; 陶書田; 施澤彬; 王迎濤; 殷豪; 秦改花 |
14 | Low temperature inhibits pollen tube growth by disruption of both tip-localized reactive oxygen species and endocytosis in Pyrus bretschneideri Rehd. | 期刊論文 | Gao, Yong-Bin(#); Wang, Chun-Lei; Wu, Ju-You; Zhou, Hong-Sheng; Jiang, Xue-Ting; Wu, Jun; Zhang, Shao-Ling(*) |
15 | Evidence for Apoplasmic Phloem Unloading in Pear Fruit | 期刊論文 | Zhang, Hu-ping(#); Wu, Ju-you; Tao, Shu-tian; Wu, Tao; Qi, Kai-jie; Zhang, Shu-jun; Wang, Ji-zhong; Huang, Wen-jiang; Wu, Jun; Zhang, Shao-ling(*) |
16 | 一種梨果實萼片狀態主效QTL位點的SNP分子標記引物及其套用 | 專利 | 吳俊; 張紹鈴; 李雷廷; 齊笑笑; 劉倫; 張明月; 謝智華 |
17 | Identification of inconsistent S-genotypes in 15 Chinese pear (Pyrus L.) cultivars | 期刊論文 | Yang, Y.(#); Xu, F. F.; Qi, K. J.; Gao, Y. B.; Xie, Z. H.; Wu, J. Y.; Zhang, S. L.(*) |
18 | Calcium treatments promote the aroma volatiles emission of pear (Pyrus ussuriensis 'Nanguoli') fruit during post-harvest ripening process | 期刊論文 | Wei, Shuwei(#); Qin, Gaihua; Zhang, Huping; Tao, Shutian; Wu, Jun; Wang, Shaomin; Zhang, Shaoling(*) |
19 | Effects of postharvest methyl jasmonate treatment on aromatic volatile biosynthesis by 'Nanguoli' fruit at different harvest maturity stages | 期刊論文 | Qin, G. H.(#); Wei, S. W.; Tao, S. T.; Zhang, H. P.; Huang, W. J.; Yao, G. F.; Sha, S. F.; Zhang, S. L.(*) |
20 | The role of sucrose-metabolizing enzymes in pear fruit that differ in sucrose accumulation | 期刊論文 | Zhang, Hu Ping(#); Wu, Ju You; Qin, Gai Hua; Yao, Gai Fang; Qi, Kai Jie; Wang, Li Fen; Zhang, Shao Ling(*) |
21 | Mitochondrial dysfunction mediated by cytoplasmic acidification results in pollen tube growth cessation in Pyrus pyrifolia | 期刊論文 | Gao, Yongbin(#); Zhou, Hongsheng; Chen, Jianqing; Jiang, Xueting; Tao, Shutian; Wu, Juyou; Zhang, Shaoling(*) |
22 | 梨己糖激酶基因PbHXK1及其套用 | 專利 | 張紹鈴; 趙碧英; 黃小三; 齊開傑 |
23 | High-density genetic linkage map construction and identification of fruit-related QTLs in pear using SNP and SSR markers | 期刊論文 | Wu, Jun(#); Li, Lei-Ting(#); Li, Meng; Khan, M. Awais; Li, Xiu-Gen; Chen, Hui; Yin, Hao; Zhang, Shao-Ling(*) |
24 | Identification of differentially expressed genes using digital gene expression profiles in Pyrus pyrifolia Nakai cv. Hosui bud release following early defoliation | 期刊論文 | Zhang, Quan-jun(#); Tao, Shu-tian; Li, Meng; Qi, Xiao-xiao; Wu, Jun; Yin, Hao; Deng, Jia-lin; Zhang, Shao-ling(*) |
25 | Identifying the candidate genes involved in the calyx abscission process of 'Kuerlexiangli' (Pyrus sinkiangensis Yu) by digital transcript abundance measurements | 期刊論文 | Qi, Xiaoxiao(#); Wu, Jun; Wang, Lifen; Li, Leiting; Cao, Yufen; Tian, Luming; Dong, Xingguang; Zhang, Shaoling(*) |
26 | Identification of miRNAs involved in pear fruit development and quality | 期刊論文 | Wu, Jun(#)(*); Wang, Defu(#); Liu, Yufeng; Wang, Long; Qiao, Xin; Zhang, Shaoling(*) |
27 | Chemical composition, crystal morphology and key gene expression of cuticular waxes of Asian pears at harvest and after storage | 期刊論文 | Wu, Xiao(#); Yin, Hao(#); Chen, Yangyang; Li, Ling; Wang, Yingzhen; Hao, Pingping; Cao, Peng; Qi, Kaijie; Zhang, Shaoling(*) |
28 | Molecular cloning and expression analysis of a gene for sucrose transporter from pear (Pyrus bretschneideri Rehd.) fruit | 期刊論文 | Zhang, Huping(#); Zhang, Shujun; Qin, Gaihua; Wang, Lifen; Wu, Tao; Qi, Kaijie; Zhang, Shaoling(*) |
項目摘要
針對梨生產中普遍存在果實風味品質較差的突出問題，我們開展了以下研究：（1）採用梨基因組重測序結果篩選基因組內均勻分布的SNP位點，構建梨果實糖、酸性狀的SNP基因晶片，實現利用基因晶片進行梨品種資源及雜種後代果實糖、酸性狀的基因分型；（2）建立利用轉錄組、蛋白質組分析及全基因組關聯分析等多種手段篩選梨果實糖、酸性狀重要候選基因的技術體系，完善轉基因番茄驗證重要候選基因功能的技術，挖掘出梨果實糖、酸性狀基因（基因家族）；（3）明確梨果實糖、酸性狀相關的重要轉錄因子、轉座子和small RNA等，揭示果實糖、酸性狀形成的‘基因-基因’互作關係及基因調控網路，闡明梨果實糖、酸性狀形成的分子及調控機制。研究結果主要包括：首先，從梨中克隆了梨果實糖轉運蛋白PbSUT、PbHT、PbSOT、PbTMT基因，己糖激酶基因PbHXK、PbFRK和山梨醇代謝相關基因 PbS6PDH、PbSDH。分析了這些基因家族的結構和進化特徵，證明了PbSUT2促進轉基因番茄成熟果實中蔗糖含量的提高，而果糖、葡萄糖和可溶性總糖含量下降；PbHT1促進轉基因番茄成熟果實中己糖和可溶性總糖含量的積累，但對蔗糖含量無顯著影響；PbSOT2的表達、NAD-SDH與NADP-SDH 的活性和己糖的積累呈正相關。PbHXK1和PbFRK1負調控果實糖積累，並影響植株的生長發育。其次，對酸積累相關基因PbALMT9、PbTDT1和PbVHA-c4研究，發現5 個ALMT家族基因與蘋果酸代謝相關，其中PbALMT9不僅轉錄表達水平高於其他基因，且表達模式與梨果實整個發育時期的蘋果酸含量變化呈正相關；PbTDT1有助於果實發育過程中有機酸的積累，超表達轉基因番茄成熟果實中的蘋果酸、延胡索酸含量高於野生型和 RNAi轉基因株系，而檸檬酸含量低於野生型和 RNAi轉基因株系；PbVHA-c4促進梨果實中檸檬酸的積累，其超表達轉基因株系的番茄果實中檸檬酸含量明顯高於野生型。研究結果不僅為梨果實品質定向改良奠定理論基礎和提供基因資源，也為分子輔助育種提供有效方法。
