(1) Man Qiao, Dan Luo, Yi Kuang, Haiyan Feng, Guangping Luo and Peng Liang. Cell Cycle Specific Distribution of Killin: Evidence for Negative Regulation of both DNA and RNA Synthesis.Cell Cycle, 2015, in Press.
(2)Xinxin Liu, Anliang Huang, Dan Luo, Haipeng Liu, Huzi Han, Yang Xu, Peng Liang.Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli Protein Expression and Purification. 2015 Feb; 109 :79–84.
(3) Ma Z,Luo D,Huang A,Xu Y,Wang Y,Wei Y,Liang P. pKILLIN: a versatile positive-selection cloning vector based on the toxicity of Killin in Escherichia coli. Gene. 2014 Jul 10; 544(2):228-35.
(4) Cho YJ, Liang P. S-phase-coupled apoptosis in tumor suppression.Cell Mol Life Sci. 2011 Jun;68(11):1883-96.
(5) He M, Liang P. IL-24 transgenic mice: in vivo evidence of overlapping functions for IL-20, IL-22, and IL-24 in the epidermis.J Immunol. 2010 Feb 15;184(4):1793-8.
(6) Cho YJ, Meade JD, Shester BR, Walden JC, Guo Z, Liang P. Linear dynamic range for signal detection in fluorescent differential display. Biotechnol Lett.2010 Nov;32(11):1593-7.
(7) Cho YJ, Meade JD, Shester BR, Walden JC, Guo Z, Liang P. Proof-reading signal accuracy of gene expression by binary differential display.Biotechnol Lett. 2010 Aug;32(8):1039-44.
(8) Meade JD,Cho YJ,Shester BR,Walden JC,Guo Z,Liang P.Automated fluorescent differential display for cancer gene profiling.Methods Mol Biol. 2010;576:99-133.
(9) S. Zhou,J.D. Meade,S. Nathashon,B. Shester,J. Walden,Z. Guo,J.Z. Liang,J. G. Liang and P. Liang.: Genome-Wide Analysis of mRNA Expression by Florescent Differential Display. The Handbook of Plant Functional Genomics. p165-186. 2008. Wiley-Blackwell publisher.
(10) Cho YJ, Liang P. Killin is a p53-regulated nuclear inhibitor of DNA synthesis. Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5396-401.
(11) Jackson, R., Cho YJ., Stein, S. andLiang, P.: CYFIP2 is a p53 target gene.Cell Cycle. 2007 Jan;6(1):95-103.
(12) Liang, P. Meade, J. and Pardee, A.B: "A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling" Nature Protocols. 2007, Mar 15, 2:457 - 470.
(13) Liang, P.: MAQC papers over the cracks. Nature Biotechnology. 2007 Jan; 25(1): 27-8.
(14) Jackson, R., Cho YJ., andLiang, P.:TIS11D is a Candidate Pro-Apoptotic p53 Target Gene. Cell Cycle. 2006 Dec;5(24):2889-2893.
(15) Liang P.:From differential display to DNA microarrays-a personal account.J Cell Physiol. 2006 Dec;209(3):653-8.
(16) Tan Z, Wang M, Liang P.: Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes. Methods Mol Biol. 2005;317:207-18.
(17) Meade JD, Cho YJ, Fisher JS, Walden JC, Guo Z, Liang P.: Automation of fluorescent differential display with digital readout. Methods Mol Biol. 2005;317:23-58.
(18) Cho YJ, Stein S, Jackson RS, Liang P.:Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display. Methods Mol Biol. 2005;317:179-92.
(19) Yang S, Liang P.: Global analysis of gene expression by differential display: a mathematical model. Methods Mol Biol. 2005;317:3-22.
(20) Thomas EK, Nakamura M, Wienke D, Isacke CM, Pozzi A, Liang P.: Endo180 binds to the C-terminal region of alpha 1(I) collagen. J Biol Chem. 2005. 280(24):22596-605.
(21) Wang M, Liang P. Interleukin-24 and its receptors. Immunology. 2005. 114(2):166-70.
(22)Yang,S. and Liang P.:Global analysis of gene expression by differential display, a mathematical model. Mol Biotechnol. 2004. 3:197-208.
(23) Wang, M., Tan,Z., Thomas, E.K. and Liang, P.: Conservation of the genomic structure and receptor-mediated signaling between human and rat IL-24. Genes & Immunity. 2004.5:363-70.
(24) Stein,S., Thomas, E.K., Herzog, B., Westfall, M. D., Rocheleau, J.V., Jackson, R., Wang, M. and Liang,P.:NDRG1 is necessary for p53-dependent apoptosis. J.Biol.Chem., 2004. 279(47): 48930-40
(25) Peng Liang & Arthur B. Pardee.: Analysing differential gene expression in cancer. Nature Reviews Cancer 2003. 3:869-876.
(26) Stein S, Cho, Y. Jackson R.S. and Liang P.: Identification of p53 target genes by fluorescent differential display. Cell Mol Life Sci. 2002 Aug;59(8):1274-9.
(27) Stein S, Liang P.: Differential display analysis of gene expression in mammals: a p53 story. Cell Mol Life Sci. 2002 Aug;59(8):1274-9.
(28) Stein J, Liang P.: Differential display technology: a general guide. Cell Mol Life Sci. 2002 Aug;59(8):1235-40. Review.
(29) Liang P.: SAGE Genie: A suite with panoramic view of gene expression. Proc.Natl.Acad.Sci.U.S.A. 2002. 99:11547-11548.
(30) Liang P.: A decade of differential display. Biotechniques. 2002. 33:338-346.
(31) Cho, Y., Prezioso, V. R. And Liang, P.: Systematic analysis of intrisince factors affecting differential display. Biotechniques 2002, 32:1-4.
(32) Wang, M.,Tan,Z., Zhang, R.,Kotenko, S. V. and Liang, P.: Interleukin-24 (Mob-5/ Mda-7) Signals through Two Heterodimeric Receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. J.Biol.Chem. 2002. 277:7341-7347.
(33) Cho, Y., Meade, J., Walden, J., Guo, Z. and Liang, P.: Multi-color Fluorescent Differential Display. Biotechniques 2001, 30:562-572.
(34) Jo, H., Cho, Y., Zhang, H. and Liang P.: Differential display analysis of altered gene expression by ras oncogene. Methods in Enzymol. (Academic Press),2001. 332: 233-244.
(36)Cho, Y. and Liang, P.: Differential display for detecting potential gene candidates. Molecular Genetics of Mental Disorders, Edited by Briley and Sulser. Martin Dunitz Publishers, London, UK.,2000. In press.
(37)Zhang, R., Tan, Z. and Liang, P.: Identification of a novel ligand-receptor pair constitutively activated by ras oncogene. J. Biol. Chem. 2000, 275:24436-24443.
(38) Jo, H., Zhang, R., Zhang, H. McKinsey, T. A. Ballard, D.W. and Liang, P.: NF-kB is required for H-Ras oncogene induced abnormal cell proliferation and tumorigenesis. Oncogene 2000, 19:841-849.
(39)Liang, P. and Pardee, A. B.: Differential Display, a general Protocol Mol Biotechnology. 1998, 10:261-267.
(40) Jo, Hakryul, Zhang, H., Zhang, R. and Liang, P.: Cloning oncogenic Ras regulated genes by differential display. Methods -A Companion to Methods in Enzymology, Academic Press, 1998, 16:365-375.
(41) Liang, P.: Factors Ensuring successful use of differential display. Methods --A Companion to Methods in Enzymology, Academic Press, 1998, 16:361-364.
(42) Sheng, H., Williams, C. S., Shao, J., Liang, P., DuBois, R. N. and Beauchamp, R. D.: Induction of cyclooxygenase-2 by activated Ha-ras oncogene in Rat-1 fibroblasts and the role of mitogen-activated protein kinase pathway. J.Biol.Chem.1998, 273: 22120-22127.
(43) Zha, ng, R., Averboukh, L., Zhu, W., Zhang, H., Jo, H., Dempsey, P.J., Coffey, R., A. B. Pardee and Liang, P.: Identification of rCop-1, a New Member of CCN Gene Family, as a Negative Regulator for Cell Transformation. Mol. Cell. Biol.,1998, 18:6131-6141.
(44) Hu, J., You, S., Li, W., Wang, D., Nagpal, M. L., Mi, Y., Liang, P. and Lin, Tu.: Expression and regulation of Interferon-g-inducible protein 10 gene in Rat Leydig Cells. Endocrinology 1998, 139:3637-3645.
(45) Liang, P., Wang, F., Zhu, W., O'Connell, R. P., and Averboukh, L.: Identification of novel diagnostic markers by differential display. Methods in Molecular Medicine. Humana Press, 1998, p3-13.
(46) Grady, T., Liang, P., Ernst,S.A.and Logsdon, C. D.: Chemokine gene expression in rat pancreatic acinar cells is an early event associated with acute pancreatitis. Gastroenterology 1997, 113:1966-1975.
(47) Liang, P. and Pardee, A.B.: Differential display: A general protocol. Methods in Molecular Biology. Humana Press. 1997, Vol. 85, p3-12.
(48) Zhang, R., Zhang, H., Zhu, W., Coffey, R., A. B. Pardee and Liang, P.: Mob-1, a ras target gene, is over expressed in colorectal cancer. Oncogene 1997, 14:1607-1610.
(49) Averboukh, L., Liang, P., Kantoff, P. W. and Pardee, A.B.: Regulation of S100P expression by androgen. Prostate, 1996, 29:350-355.
(50) Zhu, W. and Liang, P.: Detection and isolation of differentially expressed genes by differential display. Methods in Molecular Biology, 1996, 68:211-220, Boultwood, J. ed. Humana Press
(51) Liang, P.: Analysis of messenger RNA by differential display. A laboratory guide to RNA, 1996, 223-236. Krieg, P.A. ed., Wiley-Liss.
(52) , Wang, F-L., Wang, Y., Wong, W-K., Liu, Y., Addivinola, F. J., Liang, P., Chen, L-B., Kantoff, P. W. and Pardee, A.B.: Two differentially expressed genes in normal human prostate tissue and in carcinoma. Cancer Res. 1996, 56:3634-3637.
(53) Zhang, H., Zhang, R. and Liang, P.: Differential screening of gene expression difference enriched by differential display. Nucleic Acids Res. , 1996, 24:2454-2456.
(54) Hu, E., Liang, P. and Spiegelman, B. M.: AdipoQ is a novel adipose-specific gene dysregulated in obesity. J.Biol Chem. 1996, 271:10697-10703.
(55) Liang, P., Averboukh, L., Zhu, W., Wang, F., Haley, T. and Pardee, A. B.: Molecular characterization of Murine thymidylate kinase gene. Cell Growth & Differentiation 1995, 6:1333-1338.
(56) Trentmann, S.M., van der Knaap, E., Kende, H., Liang, P. and Pardee A.B.: Alternatives to 35S as a Label for the differential display of eukaryotic messenger RNA. Science 1995, 267: 1186-1187.
(57) Rohrwild, M., Alpan, R.S., Liang, P. and Pardee, A.B.: Inosine-containing primers for mRNA differential display. Trend in Genet., 1995,11:300.
(58) Liang, P. and Pardee, AB.: Recent Advances in Differential Display. Current Opinion in Immunol. 1995, 7:274-280.
(59) Liang, P., Bauer, D., Averboukh, L., Warthoe, P. Rohrwild, M., Muller, H., Strauss, M. and Pardee, A.B.: Analysis of altered gene expression by differential display. in Oncogene Techniques, Methods in Enzymol. (Academic Press),1995, 254:304-321.
(60) Liang, P., Zhu, W., Zhang, X., Guo, Z., O’Connell, R., Averboukh, L. Wang, F. and Pardee, A. B.: Differential display using one-base anchored oligo-dT primer. Nucleic Acids Res., 1994, 22:5763-5764.
(61) Liang, P. and Pardee, A.B., : Differ, ential display of mRNA by PCR. in Current Protocols in Molecular Biology (Wiley Interscience), 1994, Unit 15.8.
(62) Liang, P., Averboukh, L. and Pardee, A. B.: Method of differential display. in Methods in Molecular Genetics (Academic Press), 1994, Vol. 5, pp3-16.
(63) Liang, P., Averboukh, L., Zhu, W. and Pardee, A. B.: Ras activation of novel genes: Mob-1 as a model. Proc Natl Acad.Sci U S.A. ,1994, 91:12515-12519.
(64) Barry M. B., Meador, B., Builerback, T., Liang, P., Glaser, M. and Philips, G. N., Jr.: The closed conformation of a highly flexible protein: the structure of E. coli adenylate kinase with bound AMP and AMPPNP. Proteins 1994, 19:183-198.
(65) Russell, M. E., Utans, U., Wallace, A. F., Liang, P., Karnovsky, M. J., Wyner, L. R., Yamshita, Y. and Chi, T.: Upregulation of Gal/GalNAc macrophage lectin in chronically rejecting rat cardiac allografts with arteriosclerosis. J.Clin.Invest.,1994, 94:722-730.
(66) Utans, U., Liang, P., Wyner, L. R., Karnovsky, M. J. and Russell, M. E.: Chronic cardiac rejection: Identification of five upregulated genes in transplanted hearts by differential mRNA display. Proc.Natl.Acad.Sci.U.S.A.1994, 91:6463-6467.
(67) Sager, R., Anisowicz, A., Neveu, M., Liang, P. and Sotiropoulou, G.: Identification by differential display of alpha 6 integrin as a candidate tumor suppressor gene. FASEB J. 1993, 7:964-970.
(68) Liang, P., Averboukh, L. and Pardee, A. B.: Distribution and cloning of eukayotic mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res. 1993, 21:3269 -3275.
(69) Liang, P. Averboukh, L., Keyomarsi, K., Sager, R. and Pardee, A.B.: Differential display and cloning of mRNAs from human breast cancer vs. mammary epithelial cells. Cancer Research 1992, 52:6966-6968.
(70) Liang, P. and Pardee, A. B.: Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 1992, 257:967-971.
(71) Liang, P., Phillips Jr., G. N. and Glaser, M.: Assignment of nucleotide binding sites and the mechanism of substrate inhibition of Escherichia coli adenylate kinase. Proteins 1991, 9:28-36.
(72) Liang, P. and Glaser, M.: Efficient cloning of a mutant adenylate-kinase-encoding gene from Escherichia coli. Gene 1990, 80:21-28.
(73) Althoff, S., Zambrowicx, B., Liang, P., Glaser, M. and Phillips Jr., G. N.: Crystalization and preliminary X-ray analysis of Escherichia coli adenylate kinase. J. Mol. Biol. 1988, 199:665-666.
(74) Liang, P. and Glaser, M.: Rapid recovery and Identification of a temperature- sensitive adenylate kinase gene from Escherichia coli. J.Cell Biol. 1988, 107:306a.
編著:
(1)Liang, P.
and Pardee, A.B.: 《Differential display Methods and Protocols》. Methods in Molecular Biology,Vol. 85, Humana Press. 1979
(2)Liang, P.: 《Current progress in differential display methods and applications》. Methods-- A Companion to Methods in Enzymology, Academic Press, Vol. 16. 1998
(3)Liang, P.
Meade, JD and Pardee, A.B.:《Differential Display Methods and Protocols》.2nd Edition.
Methods in Molecular Biology, Vol. 317, Humana Press. 2005.
獲獎:
1997 獲一年一度的北美華人生物學家年會頒發的“科技發明大獎”
1998 獲二年一度的德國分子生物學和生物化學協會頒發的“分子分析大獎”
2009 中組部2009年第一批“千人計畫”入選者
2009 四川省第一批“百人計畫”入選者
2009 國務院僑辦“重點華僑華人創業團隊”獲得者
2009 國務院僑辦頒布的第二屆百名華僑華人專業人士“傑出創業獎”
專利:
(A)基因差異顯示 (Differential Display) 技術發明(四項美國專利)
1993-11-16,Arthur Pardee,Liang Peng;Identifying, isolating and cloning messenger RNAs;USA;5, 262, 311
1997-2-4,Liang Peng,Arthur Pardee;Methods of differential display of exposed mRNA by RT/PCR;USA;5, 599, 672
1997-9-9,Arthur Pardee,Liang Peng;Methods of Comparing Levels or Amount of mRNAs;USA;5, 665,547
1999-8-12,Arthur Pardee,Liang Peng;System for Comparing level or amounts of mRNA;USA;5,965,409
(B)蛋白質三聚體化(Trimer-Tag)技術發明(三項美國專利)
2007-9-11,Liang Peng;Methods and Compositions For Producing Secreted Trimeric Receptor Analogs And Biologically Active Fusion Proteins;USA;7,268,116
2010-2-23,Liang Peng;Methods and Compositions For Producing Secreted Trimeric Receptor Analogs And Biologically Active Fusion Proteins;USA;7,666,837
2010-4-6,Liang Peng;Methods for Blocking TNF-alpha Activity in Mammals with Trimeric Soluble TNF Receptors;USA;7,691,815