李典範

簡歷：

1.1995/09-1999/06湖南農業大學，學士

2.1999/09-2002/06湖南農業大學，碩士

3. 2002/09-2006/12武漢大學，博士

4. 2006/12-2014/10愛爾蘭利默里克大學；都柏林聖三一學院，博士後

5. 2014/11-至今中國科學院上海生命科學研究院，生物化學與細胞生物學研究所，國家蛋白質科學研究中心。研究員，課題組長，博士生導師

研究方向：

膜蛋白結構功能及其前沿技術

職稱：

研究員

職務：

研究組長、博士生導師

代表論著：

1. D Li, JA Lyons, VE Pye, L Vogeley, D Aragao, C Kenyon, ST Shah, C Doherty, M Aherne, M Caffrey. Crystal structure of the integral membrane diacylglycerol kinase. Nature 2013 497:521-4

2. D Li, M Caffrey. Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes. Proc. Natl. Acad. Sci. USA 2011 108:8639-44

3. D Li, VE Pye, M Caffrey. Experimental phasing for structure determination using membrane protein crystals grown by the lipid cubic phase method. Acta Crystallogr. D. 2015 71:104-122

4. D Li, M Caffrey. Renaturing Membrane Proteins in the Lipid Cubic Phase, a Nanoporous Membrane Mimetic. Sci. Rep. 2014 4:5806

5. D Li, N Howe, A Dukkipati, STA Shah, BD Bax, C Edge, A Bridges, P Hardwick, OMP Singh, A Pautsch, R Pfau, G Schnapp, M Wang, V Olieric,

M Caffrey. Crystallizing membrane proteins in the lipidic mesophase. Experience with human prostaglandin E2 synthase 1 and an evolving strategy. Cryst. Growth Des. 2014 14:2037-2047

6. D Li, ST Shah, M Caffrey. Host lipid and temperature as important screening variables for crystallizing integral membrane proteins in lipidic

mesophases. Trials with diacylglycerol kinase. Cryst. Growth Des. 2013 13:2846-57

7. D Li, J Lee, M Caffrey. Crystallizing membrane proteins in lipidic mesophases. A host lipid screen. Cryst. Growth Des. 2011 11:530-7

8. J Tan*, SL Rouse*, D Li* (co-first author), VE Pye, L Vogeley, A Brinth, JC Whitney, PL Howell, MSP Sansom, M Caffrey. A conformational

landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa. Acta Crystallogr. D. 2014. 70:2054-68

9. C Boland, D Li* (co-first author), STA Shah, S Haberstock, V D?tsch, F Bernhard, M Caffrey. Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination. Cell. Mol. Life Sci. 2014 71:4895-910

10. W Liu, D Wacker, C Gati, GW Han, D James, D Wang, G Nelson, U Weierstall, V Katritch, A Barty, NA Zatsepin, D Li, M Messerschmidt, S

Boutet, GJ Williams, JE Koglin, MM Seibert, C Wang, ST Shah, S Basu, R Fromme, C Kupitz, KN Rendek, I Grotjohann, P Fromme, RA Kirian, KR

Beyerlein, TA White, HN Chapman, M Caffrey, JC Spence, RC Stevens, V Cherezov. Serial femtosecond crystallography of G protein-coupled

receptors. Science 2013 342:1521-4

11. T Weinert, V Olieric, S Waltersperger, E Panepucci, L Chen, H Zhang, D Zhou, J Rose, A Ebihara, S Kuramitsu, D Li, N Howe, G Schnapp, A

Pautsch, K Bargsten, AE Prota, P Surana, J Kottur,DT Nair, F Basilico, V Cecatiello, S Pasqualato, A Boland, O Weichenrieder, B Wang, MO

Steinmetz, M Caffrey, M Wang. Fast native-SAD phasing for routine macromolecular structure determination. Nat. Methods. 2014 In press

12. U Weierstall, D James, D Wang, W Liu, JCH Spence, RB Doak, G Nelson, P Fromme, R Fromme, I Grotjohann, C Kupitz, NA Zatsepin, S Basu, D Wacker, C Wang, S Boutet, M Messerschmidt, GJ Williams, JE Koglin,MM Seibert, C Gati, RL Shoeman, A Barty, HN Chapman, RA Kirian, KR Beyerlein, RC Stevens, D Li, STA Shah, N Howe, M Caffrey, V Cherezov. Lipidic cubic phase injector facilitates membrane protein serial

femtosecond crystallography. Nat. Commun. 2014. 5: 3309

13. JT Hopper, YT Yu, D Li, A Raymond, M Bostock, I Liko, V Mikhailov, A Laganowsky, JL Benesch, M Caffrey, D Nietlispach, CV Robinson.

Detergent-free mass spectrometry of membrane protein complexes. Nat. Methods 2013 10: 1206-8

14. JA Lyons, JL Parker, N Solcan, A Brinth, D Li, S.T.A. Shah, M Caffrey, S Newstead. Structural basis for polyspecificity in the POT family of proton

coupled oligopeptide transporters. EMBO Rep. 2014 15: 886-93

15. E Nji, D Li, DA Doyle, M Caffrey. Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa. Acta. Crystallogr. F 2014 70:1362-7

16. M Caffrey, D Li, N Howe, STA Shah. Hit and run’serial femtosecond crystallography of a membrane kinase in the lipid cubic phase. Phil Trans B. 2014. 369: 20130621

17. M Caffrey, D Li, A Dukkipati. Membrane protein structure determination using crystallography and lipidic mesophases: Recent advances and successes. Biochemistry 2012 51:6266-88

18. M Caffrey, R Eifert, D Li, N Howe. The lipid cubic phase or in meso method for crystallizing proteins. Bushings for better manual dispensing. J.Appl. Crystallogr. 2014 47:1804-6

19. D Li, C Boland, K Walsh, M Caffrey. Use of a robot for high-throughput crystallization of membrane proteins in lipidic mesophases. J. Vis. Exp. 2012 67:e4000

20. D Li, C. Boland, D Aragao, K Walsh, M Caffrey. Harvesting and cryo-cooling crystals of membrane proteins grown in lipidic mesophases for structure determination by macromolecular crystallography. J. Vis. Exp. 2012 67:e4001

21. I Nederlof, R Hosseini, D Georgieva, J Luo, D Li, JP Abrahams. A straightforward and robust method for introducing human hair as a nucleant into high throughput crystallization trials. Cryst. Growth Des. 2011 11:1170-6

李典範

基本介紹

相關詞條

熱門詞條