《從基因到基因組——DNA技術概念和套用》是2004年科學生物分社出版的圖書，作者是J.W.戴爾、M.馮尚茨。
基本信息,內容簡介,圖書目錄,
基本信息
現代生物技術前沿 | ||
J.W.戴爾[美],M.馮尚茨[美] 著 | ||
科學出版社 | 2004年3月出版 | |
定價：46.00 | 語種：英文 | |
標準書號：7-03-012477-4 | 裝幀：平裝 | |
版本：第一版 影印版 | 開本：B5 | |
責任編輯： | 字數：454千字 | |
讀者對象：本科以上文化程度 | 頁數：360 | |
書類：理論專著/套用技術 | 冊/包：7 | |
編輯部： 科學生物分社 | ||
附註： | ||
內容簡介
基因技術、遺傳工程、重組DNA和基因克隆等技術的快速發展，把分子生物學推到了生命科學的前沿。本書對分子生物學核心技術的原理及其使用進行了簡明扼要的闡釋。以對分子生物學的基本概念的簡短介紹開篇，然後介紹核心的分子生物學方法及其整合運用，從單個基因的克隆與研究到整個基因組的測序，以及基因組信息的分析。最後，本書還介紹了這些技術在生物技術、醫藥和農業以及整個生命科學研究中的套用。
本書內容涵蓋了分子生物學基礎、基因克隆、核酸的純化與分離、DNA的剪下與連線、載體、基因組文庫和cDNA文庫、聚合酶鏈反應、DNA測序、序列數據分析、基因變異分析、基因表達分析、基因功能分析、醫藥套用、轉基因等等方面。
本書適於生物化學、分子生物學、遺傳學、細胞生物學、生物技術、生物工程等相關學科及研究領域的本科生、研究生及教學科研人員參考使用。
本書內容涵蓋了分子生物學基礎、基因克隆、核酸的純化與分離、DNA的剪下與連線、載體、基因組文庫和cDNA文庫、聚合酶鏈反應、DNA測序、序列數據分析、基因變異分析、基因表達分析、基因功能分析、醫藥套用、轉基因等等方面。
本書適於生物化學、分子生物學、遺傳學、細胞生物學、生物技術、生物工程等相關學科及研究領域的本科生、研究生及教學科研人員參考使用。
圖書目錄
Preface
1 Introduction
2 Basic Molecular Biology
2.1 Nucleic Acid Structure
2.2 Gene Structure and Organization
2.3 Information Flow:Gene Expression
3 How to Clone a Gene
3.1 What is Cloning?
3.2 Wverview of the Procedures
3.3 Gene Libraries
3.4 Hybridization
3.5 Polymerase Chain Reaction
4 Purification and Separation of Nucleic Acids
4.1 Extraction and Purification of Nucleic Acids
4.2 Detection and Quantitation of Nucleic Acids
4.3 Gel Electrophoresis
5 Cutting and Joining DNA
5.1 Restriction Endonucleases
5.2 Ligation
5.3 Alkaline Digests
5.4 Double Digests
5.5 Modification of Restriction Fragment Ends
5.6 Other Ways of Joining DNA Molecules
5.7 Summary
6 Vectors
6.1 Plasmid Vectors
6.2 Vectors Based on the Lambda Bacteriophage
6.3 Cosmids
6.4 M13 Vectors
6.5 Expression Vectors
6.6 Vectors for Cloning and Expression in Eukaryotic Cells
6.7 Supervectors:YACs and BACs
6.8 Summary
7 Genomic and cDNA Libraries
7.1 Genomic Libraries
7.2 Growing and Storing Libraries
7.3 cDNA Libraries
7.4 Random,Arrayed and Ordered Libraries
8 Finding the Right Clone
8.1 Screening Libraries with Gene Probes
8.2 Screening Expression Libraries with Antibodies
8.3 Rescreening
8.4 Subcloning
8.5 Characterization of Plasmid Clones
9 Polymerase Chain Reaction(PCR)
9.1 The PCR Reaction
9.2 PCR in Practice
9.3 Cloning PCR Products
9.4 Long-range PCR
9.5 Reverse-transcription PCR
9.6 Rapid Amplification of cDNA Ends(RACE)
9.7 Applications of PCR
10 DNA Sequencing
10.1 Principles of DNA Sequencing
10.2 Automated Sequencing
10.3 Extending the Sequence
10.4 Shotgun Sequencing:Contig Assembly
10.5 Genome Sequencing
11 Analysis of Sequence Data
11.1 Analysis and Annotation
11.2 Databanks
11.3 Sequence Comparisons
12 Analysis of Genetic Variation
12.1 Nature of Genetic Variation
12.2 Methods for Studying Variation
13 Analysis of Gene Expression
13.1 Analysing Transcription
13.2 Comparing Transcriptomes
13.3 Methods for Studying the Promoter
13.4 Translational Analysis
14 Analysis of Gene Function
14.1 Relating Genes and Functions
14.2 Genetic Maps
14.3 Relating Genetic and Physical Maps
14.4 Linkage Analysis
14.5 Transposon Mutagenesis
14.6 Allelic Replacement and Gene Knock-outs
14.7 Complementation
14.8 Studying Gene Function through Protein Interactions
15 Manipulating Gene Expression
15.1 Factors Affecting Expression of Clonde Genes
15.2 Expression of Cloned Genes in Bacteria
15.3 Expression in Eukaryotic Host Cells
15.4 Adding Tags and Signals
15.5 In vitro Mutagenesis
16 Medical Applications,Present and Future
16.1 Vaccines
16.2 Detection and Identification of Pathogens
16.3 Human Genetic Diseases
17 Transgenics
17.1 Transgenesis and Cloning
17.2 Animal Transgenesis and its Applications
17.3 Transgenic Plants and their Applications
17.4 Summary
Bibliography
Glossary
Index
1 Introduction
2 Basic Molecular Biology
2.1 Nucleic Acid Structure
2.2 Gene Structure and Organization
2.3 Information Flow:Gene Expression
3 How to Clone a Gene
3.1 What is Cloning?
3.2 Wverview of the Procedures
3.3 Gene Libraries
3.4 Hybridization
3.5 Polymerase Chain Reaction
4 Purification and Separation of Nucleic Acids
4.1 Extraction and Purification of Nucleic Acids
4.2 Detection and Quantitation of Nucleic Acids
4.3 Gel Electrophoresis
5 Cutting and Joining DNA
5.1 Restriction Endonucleases
5.2 Ligation
5.3 Alkaline Digests
5.4 Double Digests
5.5 Modification of Restriction Fragment Ends
5.6 Other Ways of Joining DNA Molecules
5.7 Summary
6 Vectors
6.1 Plasmid Vectors
6.2 Vectors Based on the Lambda Bacteriophage
6.3 Cosmids
6.4 M13 Vectors
6.5 Expression Vectors
6.6 Vectors for Cloning and Expression in Eukaryotic Cells
6.7 Supervectors:YACs and BACs
6.8 Summary
7 Genomic and cDNA Libraries
7.1 Genomic Libraries
7.2 Growing and Storing Libraries
7.3 cDNA Libraries
7.4 Random,Arrayed and Ordered Libraries
8 Finding the Right Clone
8.1 Screening Libraries with Gene Probes
8.2 Screening Expression Libraries with Antibodies
8.3 Rescreening
8.4 Subcloning
8.5 Characterization of Plasmid Clones
9 Polymerase Chain Reaction(PCR)
9.1 The PCR Reaction
9.2 PCR in Practice
9.3 Cloning PCR Products
9.4 Long-range PCR
9.5 Reverse-transcription PCR
9.6 Rapid Amplification of cDNA Ends(RACE)
9.7 Applications of PCR
10 DNA Sequencing
10.1 Principles of DNA Sequencing
10.2 Automated Sequencing
10.3 Extending the Sequence
10.4 Shotgun Sequencing:Contig Assembly
10.5 Genome Sequencing
11 Analysis of Sequence Data
11.1 Analysis and Annotation
11.2 Databanks
11.3 Sequence Comparisons
12 Analysis of Genetic Variation
12.1 Nature of Genetic Variation
12.2 Methods for Studying Variation
13 Analysis of Gene Expression
13.1 Analysing Transcription
13.2 Comparing Transcriptomes
13.3 Methods for Studying the Promoter
13.4 Translational Analysis
14 Analysis of Gene Function
14.1 Relating Genes and Functions
14.2 Genetic Maps
14.3 Relating Genetic and Physical Maps
14.4 Linkage Analysis
14.5 Transposon Mutagenesis
14.6 Allelic Replacement and Gene Knock-outs
14.7 Complementation
14.8 Studying Gene Function through Protein Interactions
15 Manipulating Gene Expression
15.1 Factors Affecting Expression of Clonde Genes
15.2 Expression of Cloned Genes in Bacteria
15.3 Expression in Eukaryotic Host Cells
15.4 Adding Tags and Signals
15.5 In vitro Mutagenesis
16 Medical Applications,Present and Future
16.1 Vaccines
16.2 Detection and Identification of Pathogens
16.3 Human Genetic Diseases
17 Transgenics
17.1 Transgenesis and Cloning
17.2 Animal Transgenesis and its Applications
17.3 Transgenic Plants and their Applications
17.4 Summary
Bibliography
Glossary
Index
