研究員，離子通道藥物研發中心海外團隊成員之一，現任美國華盛頓大學生物醫學工程系教授。1992年在美國紐約州立大學（石溪分校）獲博士學位，隨後又在美國紐約州立大學（石溪分校）和史丹福大學從事博士後研究工作，1999年開始先後在凱斯西儲大學和華盛頓大學任副教授，2010年任美國華盛頓大學生物醫學工程系教授。工作期間，榮獲各項榮譽，其中包括美國心臟協會Established Investigator獎，被提名2003年Carl F．Wittke傑出本科教學工作者，還多次擔任國際學術會議專題主席。在Nature、Neuron、PNAS等國際著名學術期刊上發表SCI論文30多篇。
基本介紹
- 中文名：崔建民
- 外文名：J Cui
- 國籍：美國
- 職業：大學教授
- 畢業院校：北京大學
- 主要成就：在Nature、Neuron、PNAS等國際著名學術期刊上發表SCI論文多篇
個人簡介,教育和工作經歷,研究領域和方向,科研成果,On BK-type Ca activated K channels,IKs channels,Ultrasound mediated drug/gene delivery,Cardiac physiology,Invited reviews,Book Chapter,
個人簡介
研究員，現任美國華盛頓大學生物醫學工程系教授，工作期間，榮獲各項榮譽，其中包括美國心臟協會Established Investigator獎，被提名2003年Carl F．Wittke傑出本科教學工作者，還多次擔任國際學術會議專題主席。在Nature、Neuron、PNAS等國際著名學術期刊上發表SCI論文30多篇。
教育和工作經歷
1979-1983 北京大學，無線電電子學系，獲理學學士學位；
1983-1986 北京大學，生物系，獲理學碩士學位；
1986-1992 美國紐約州立大學石溪分校，生理學及生物物理學系，博士；
1992-1994 美國紐約州立大學石溪分校，生理學及生物物理學系，博士後；
1994-1999 美國加利福尼亞州史丹福大學，分子及細胞生理學系，博士後；
1999-2004 美國凱斯西儲大學，生物醫學工程系，助理教授；
2004-2010 美國華盛頓大學，生物醫學工程系，副教授；
2005-至今 美國華盛頓大學，細胞生物學和生理學，及化學工程系，副教授；
2010-至今 美國華盛頓大學，生物醫學工程系，教授；
2011-至今 中國科學院昆明動物所研究員。
1983-1986 北京大學，生物系，獲理學碩士學位；
1986-1992 美國紐約州立大學石溪分校，生理學及生物物理學系，博士；
1992-1994 美國紐約州立大學石溪分校，生理學及生物物理學系，博士後；
1994-1999 美國加利福尼亞州史丹福大學，分子及細胞生理學系，博士後；
1999-2004 美國凱斯西儲大學，生物醫學工程系，助理教授；
2004-2010 美國華盛頓大學，生物醫學工程系，副教授；
2005-至今 美國華盛頓大學，細胞生物學和生理學，及化學工程系，副教授；
2010-至今 美國華盛頓大學，生物醫學工程系，教授；
2011-至今 中國科學院昆明動物所研究員。
研究領域和方向
離子、藥物、基因的膜通透性，主要包括兩方面：
離子通道發揮功能的分子機制，與疾病相關的離子通道突變的分子/細胞機制，及對離子通道有調控作用的小分子;超聲介導的藥物/基因的傳送。
離子通道發揮功能的分子機制，與疾病相關的離子通道突變的分子/細胞機制，及對離子通道有調控作用的小分子;超聲介導的藥物/基因的傳送。
科研成果
On BK-type Ca activated K channels
20. Li M, Chang S, Yang L, Shi J, McFarland K, Yang X, Moller A, Wang C, Zou X, Chi C, Cui J. (2014) Conopeptide Vt3.1 preferentially inhibits BK potassium channels containing β4 subunits via electrostatic interactions. J Biol Chem. 289(8):4735-42. doi: 10.1074/jbc .M113.535898
19. Sun X, Shi J, Delaloye K, Yang X, Yang H, Zhang G, and Cui J (2013) The interface between membrane- spanning and cytosolic domains in BK channels is involved in β subunits modulation of gating. J Neurosci. 33:11253-11261. PMCID: in process
18. Yang, J., Delaloye, K., Yang, X., Moller, A., Shi, J. and Cui, J. (2013) Interactions between structural domains during BK channel gating. J. Gen. Physiol. 141:217-228. doi:10.1085/jgp.201210794 .
17. Deng, P.Y., Rotman, Z., Blundon, J.A., Cho Y., Cui, J., Cavalli, V.M., Zakharenko, S.S., and Klyachko, V.A. (2012) FMRP Regulates Neurotransmitter Release and Synaptic Information Transmission by Modulating Action Potential Duration via BK channels. Neuron 77:696-711. PMCID: 3584349
16. Yang, J., Delaloye, K., Lee, U.S., and Cui, J. (2011) Patch clamp and perfusion techniques for studying ion channels expressed in Xenopus oocytes. J Vis Exp. 47, pii: 2269. doi: 10.3791/2269. 15. Zhang, G., Huang, S.Y., Yang, J. Shi, J., Yang, X., Moller, A., Zou, X., and Cui, J. (2010) Ion sensing in the RCK1 domain of BK channels. Proc. Natl. Acad. Sci., U.S.A. 107:18700-18705. PMCID: 2972974.
14. Lee, U.S., Shi, J. and Cui, J. (2010) Modulation of BK channel gating by the Î22 subunit involves both membrane-spanning and cytoplasmic domains of Slo1. J Neurosci. 30:16170-16179. PMCID: 3073600.
13. Yang, J,, Krishnamoorthy, G., Saxena, A., Zhang, G., Shi, J., Yang, H., Delaloye, K., Sept, D., and Cui, J. (2010) An epilepsy/dyskinesia-associated mutation enhances BK channel activation by potentiating the Ca2+ dependent allosteric mechanism. Neuron 66:871-883. PMCID: 2907746 [Previews: Neuron 66, 817- 818].
12. Lee, U.S. and Cui, J. (2009) β subunit-specific modulations of a mutant BK channel associated withepilepsy and dyskinesia. J. Physiol. (London) 587:1481-1498. PMCID: 2678220
11. Yang, H., Shi, J., Zhang, G., Yang, J, Delaloye, K., and Cui, J. (2008) Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and the RCK1 domains Nature Structure and Molecular Biology 15:1152-1159. PMCID: 2579968 [News & Views: Nature Structure and Molecular Biology 15:1130 -1132].
10. Yang, H., Zhang, G., Shi, J., Lee, U.S., Delaloye, K., and Cui, J. (2008) Subunit-specific Effect of the Voltage Sensor Domain on Ca2+ Sensitivity of BK Channels. Biophys. J., 96:4678-4687. PMCID: 2397326
9. Yang, H., Hu, L., Shi, J., Delaloye, K., Horrigan, F., and Cui, J. (2007) Mg2+ Mediates Interaction between the Voltage-Sensor and Cytosolic Domain to Activate BK channels. Proc. Natl. Acad. Sci., U.S.A. 104, 18270-18275. PMCID: 2084332 [Commentary: J. Gen. Physiol. 131, 5-11]
8. Yang, H., Hu, L., Shi, J., and Cui, J. (2006) Tuning magnesium sensitivity of BK channels by mutations. Biophys. J., 91, 2892-2900. PMCID: 1578465
7. Lei Hu, Huanghe Yang, Jingyi Shi, and Jianmin Cui (2006) Effects of multiple metal binding sites on calcium and magnesium-dependent activation of BK channels. J. Gen. Physiol. 127, 35-49. PMCID: 2151482
6. Krishnamoorthy, G., Shi, J., Sept, D., and Cui, J. (2005) The N-terminus of RCK1 domain regulates Ca2+ dependent BKCa channel gating. J. Gen. Physiol. 126, 227-241. PMCID: 2266574
5. Du, W., Bautista, J. F., Yang, H., Diez-Sampedro, A., You, S.-A., Wang, L., Kotagal, P., Lüders, H. O., Shi,J., Cui, J., Richerson, G. B., and Wang, Q. (2005) Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder. Nat. Genet. 37, 733-738
4. Guoxia Liu, Jingyi Shi, Lin Yang, Luxiang Cao, Soo Mi Park, Jianmin Cui, Steven O. Marx (2004) Assembly of a Ca2+-dependent BK channel signaling complex by binding to β2 adrenergic receptor. EMBO J 23, 2196-2205. PMCID: 419908
3. Lei Hu, Jingyi Shi, Zhongming Ma, Gayathri Krishnamoorthy, Fred Sieling, Guangping Zhang, Frank Horrigan and Jianmin Cui (2003) Participation of the S4 Voltage Sensor in the Mg2+-Dependent Activation of Large Conductance (BK) K+ Channels. Proc. Natl. Acad. Sci., U.S.A. 100, 10488-10493. PMCID: 193588
2. Shi, J. Krishnamoorthy, G., Yang, Y., Hu, L., Chaturvedi, N., Harilal, D., Qin, J., and Cui, J. (2002). Mechanism of magnesium activation of calcium-activated potassium channels. Nature 418, 876-880.
1. Shi, J. and Cui, J. (2001). Intracellular Mg2+ enhances the function of BK-type Ca2+-activated K+channels. J. Gen. Physiol. 118, 589-605. PMCID: 2233844 [Commentary: J. Gen. Physiol. 118, 583-587].
IKs channels
7. Li Y, Gao J, Lu Z, Delaloye D, Shi J, Bock K, Cohen IS, and Cui J (2013) Intracellular ATP binding isÂÂrequired to activate the slow activating K+ channel IKs.Proc. Natl. Acad. Sci., U.S.A. (in press)
6. Zaydman MA, Silva JR, Delaloye K, Li Y, Liang H, Larsson HP, Shi J, and Cui J (2013) Kv7.1 ion channels require PIP2 to couple voltage sensing to pore opening. Proc. Natl. Acad. Sci., U.S.A. Â110:13180-13185. PMCID: in process
5 . Lee H-C, Rudy Y, Chen P-Y, Sheu S-H, Chang J-G and Cui J (2013) Modulation of KCNQ1 Alternative Splicing Regulates Cardiac IKs Currents and Action Potential Repolarization. HeartRhythm 10:1220-1228. PMCID: in process
4. Li, Y., Zaydman, M.A., Wu, D., Shi, J., Guan, M., Virgin-Downey, B.W., and Cui, J. (2011) KCNE1 enhances PIP2 sensitivity of IKs to modulate channel activity. Proc. Natl. Acad. Sci., U.S.A. 108:9095-9100. PMCID: PMC3107281
3. Wu, D., Pan, H., Delaloye, K., and Cui, J. (2010) KCNE1 remodels the voltage sensor of Kv7.1 to modulate channel function. Biophys. J. 99:3599-3608. PMCID: 2998620
2. Wu, D., Delaloye, K., Zaydman, M.A., Nekouzadeh, A., Rudy, Y., and Cui, J. (2010) State dependent electrostatic interactions of S4 arginines with E1 in S2 during Kv7.1 activation.J. Gen. Physiol. 135:595- 606. PMCID: 2888051
1. Silva, J., Pan, H., Wu, D., Nekouzadeh, A., Decker, K., Cui, J., Baker, N., Sept, D., and Rudy, Y. (2009) Linking Ion-Channel Molecular Dynamics to the Action Potential: Cardiac IKs Simulations. Proc. Natl. Acad. Sci., U.S.A., 106:11102-11106. PMCID: 2700153
Ultrasound mediated drug/gene delivery
6. Zhou Y., Yang K., Cui J., Ye J.Y., Deng C.X. (2012) Controlled permeation of cell membrane by single bubble acoustic cavitation.J Control Release 157:103-111.
5. Zhou, Y., Kumon, R.E., Cui, J. and Deng, C.X. (2009) The Size of Sonoporation Pores on the CellMembrane. Ultrasound in Medicine and Biology 35:1756-1760. PMCID: 2752487
4. Zhou, Y., Cui, J. and Deng, C.X. (2008) Dynamics of Sonoporation Correlated with Acoustic Cavitation Activities.Biophys. J., 94:L51-53. PMCID: 2267145
3. Zhou, Y., Shi, J., Cui, J. and Deng, C.X. (2007) Effects of extracellular calcium on cell membrane resealing in sonoporation. J. Control. Release. 126:34-43. PMCID: 2270413
2. Pan, H., Zhou, Y., Izadnegahdar, O., Cui, J., and Deng, C. X. (2005) Study of Sonoporation Dynamics Affected by Ultrasound Duty Cycle. Ultrasound in Medicine and Biology. 31, 849-856. PMCID: 1182120
1. Cheri Deng, Fred Sieling, Hua Pan, and Jianmin Cui (2004) Ultrasound-induced cell membrane porosity. Ultrasound in Medicine and Biology. 30, 519-526.
Cardiac physiology
2. Li, J., Yan B., Huo Z., Liu Y., Xu J., Sun Y., Liu Y., Liang D., Peng L., Zhang Y., Zhou Z.N., Shi J., Cui J., Chen Y.H. (2010) {beta}2- but not {beta}1-adrenoceptor activation modulates intracellular oxygen availability. J. Physiol. 15:2987-2998.
1. Yiqing Yang, Min Xia, Qingfeng Jin, Said Bendahhou, Jingyi Shi, Yiping Chen, Bo Liang, Jie Lin, Yi Liu, Ban Liu, Qinshu Zhou, Dongwei Zhang, Rong Wang, Ning Ma, Xiaoyan Su, Kaiya Niu, Yan Pei, Wenyuan Xu, Zhaopeng Chen, Haiying Wan, Jianmin Cui, Jacques Barhanin, and Yihan Chen (2004) Identification of a KCNE2 Gain-of-Function Mutation in Familial Atrial Fibrillation. Am. J. Hum. Genet. 75:899-905. PMCID: 1182120
Invited reviews
6. Zaydman, M.A., Silva, J.R., and Cui, J. (2012) Ion channel associated diseases: overview of molecular mechanisms. Chem Rev dx.doi.org/10.1021/cr300360k
5. Sun, X., Zaydman, M.A., and Cui, J. (2012) Regulation of KV channel voltage-dependent activation by transmembrane β subunits.Front. Pharmacol. 3:63. doi: 10.3389/fphar.2012.00063. PMCID: 3328208
4. Cui, J. (2010) BK-type calcium-activated potassium channels: coupling of metal ions and voltage sensing.J. Physiol. 588:4651-4658. PMCID: 3010134
3. Lee, U.S. and Cui, J. (2010) BK channel activation: structural and functional insights. Trends in Neurosciences 33:415-423. PMCID: 2929326.
2. Cui, J. (2010) Reduction of CaV channel activities by Ca2+-CaM: inactivation or deactivation? J. Gen. Physiol. 135:297-301. PMCID: 2847919
1. Cui, J., Yang, H., and Lee, U.S. (2009) Molecular mechanisms of activation of Ca2+ activated K+ channels. Cellular and Molecular Life Sciences, 66:852-875. PMCID: 2694844.
Book Chapter
1. Wu, D. and Cui, J. (2012). Ion channels in the cell membrane: structure, function and modeling. In Comprehensive Biomedical Physics, Anders Brahme eds, Elsevier. In press
