孔五一，美籍華人，1953年5月1日出生在內蒙古呼和浩特市，是蒙古族。美國史丹福大學資深研究員，復旦大學客座教授，內蒙古自治區“草原英才”獲得者之一，北京喀薩爾醫學科技有限公司首席科學家，內蒙古弘泰醫學科技有限公司合伙人。她主要從事血液細胞分子生物學以及生物技術的研究與開發工作，在細胞和組織再生、幹細胞、巨核細胞等方面的研究過程中取得許多國際領先的學術成果。
基本介紹
- 中文名：孔五一
- 外文名：W. Kong
- 別名：無
- 國籍：美國
- 出生地：內蒙古
- 出生日期：1953年5月1日
- 職業：首席科學家
- 畢業院校：美國加州大學洛杉磯分校外科
- 信仰：無
- 主要成就：再生粒子的研發
申請獲得的專利 - 專利：RNA胞體及其用途
- 專利：REGENE RATIVE DOT CELLS
人物介紹
孔五一，美籍華人，醫學，理學博士。美國史丹福大學資深研究員，復旦大學客座教授，內蒙古醫科大學客座教授，內蒙古自治區“草原英才”獲得者之一，內蒙古科技廳聘外國專家，外聘教授，北京喀薩爾醫學科技有限公司首席科學家，內蒙古弘泰醫學科技有限公司合伙人。從事血液細胞分子生物學以及生物技術的研究與開發工作，在細胞和組織再生、幹細胞、巨核細胞等方面的研究過程中取得許多國際領先的學術成果。
孔五一教授1980年從北京醫科大學醫學專業本科畢業後回到呼和浩特，在內蒙古醫學院任助教一職。
1985年，孔五一教授去了美國，在美國亞利桑那大學醫學院生理系任研究員，於1992年取得理學博士學位。1993年，孔五一教授轉到加州大學舊金山分校外科，於96年取得博士後學位，並任外科研究員。1999年，孔五一教授來到加州大學洛杉磯分校外科任科研助教，於2001年轉到美國史丹福大學外科，自此開始了她在史丹福大學外科長達十年的資深研究員工作。
研究方向
再生醫學： 在5年前發現了非血小板核糖核酸粒子， 經過五年的研究發現非血小板核糖核酸粒子可以再造各種細胞，可望給各種病人帶來治癒的可能。
研究領域主要集中於：非血小板核糖核酸粒子的發現及功能鑑定；非血小板核糖核酸粒子在皮膚損傷中使疤痕減少的機理；非血小板核糖核酸粒子在糖尿病鼠皮膚損傷後使傷口癒合的功能；非血小板核糖核酸粒子修復冠狀動脈缺血引起的心肌壞死的機理；非血小板核糖核酸粒子恢復肝硬化引起的肝細胞壞死的機理；非血小板核糖核酸粒子恢復損傷的神經細胞的機理；非血小板核糖核酸粒子的RNA功能分析等。
主要學術成就
（1）發現胰蛋白酶在小腸細胞表面是通過調節PAR-2起作用
雖然腸道的消化酶的存在是早已定論的事情。但是這些酶是如何調節腸細胞的活動並不清楚。孔五一教授在1997年發現腸道細胞表面存在一種受體（PAR-2），而PAR-2與體內其他受體不同。PAR-2 是被胰蛋白酶斷解之後才具有活性。而被活化的PAR-2可以進一步調節腸細胞的吸收功能。孔五一教授的研究結果發表在美國科學院雜誌(PNAS)上。此文章已被廣泛引用，多達217次。
（2）首次發現Cyclophilin-C結合蛋白（CyCAP）在慢性炎症中的作用
現在大家所接受的動脈硬化發病，老年痴呆症，以及其他老年病等學說基本上是由於慢性炎症反應學說。孔五一教授在研究中發現CyCAP可以調節體內引發炎症的多肽TNF-alpha 的釋放。孔五一教授已連續發表了數篇有關CyCAP的文章。對慢性炎症反應發生的機理研究做出了重大貢獻。
（3）第一個發現皮膚內的KPRP基因
孔五一教授於2002年在史丹福大學發現一個從未被報導過的基因，通過使用基因庫的分析，孔五一教授的研究結果除發表在專業雜誌上並在世界上第一個繪出了此基因的人的蛋白質圖譜。這項研究成果為全球的基因庫及蛋白庫又增加了一份基本的數據。
（4）第一個發現非血小板核糖核酸粒子
孔五一教授在2005前發現了非血小板核糖核酸粒子。這項發現解決了目前幹細胞研究領域的很多沒有解決的問題。這些問題包括1）為什麼骨髓幹細胞在培養後很快分化為間充質樣細胞，並喪失幹細胞的表面標誌? 2）幹細胞是如何擴增的? 有人認為幹細胞是通過不對稱分裂來擴增的。但是這個理論並沒有數據支持。 3）幹細胞是如何分化的? 雖然幹細胞有多分化特性，但它們的分化基理到目前也不清楚。孔五一教授發現的非血小板核糖核酸粒子解決了以上所提出的各項幹細胞領域的問題。孔五一教授認為，非血小板核糖核酸粒子是形成幹細胞的物質。幹細胞本身不是體內最原始的物質，它們是由非血小板核糖核酸粒子融合而產生的。非血小板核糖核酸粒子可以形成各種種類的幹細胞，而幹細胞的多分化機理即非血小板核糖核酸粒子的多分化機理。此學說於發表在Clinical Experimental Pharmacology and Physiology雜誌上。很多同行專家認為非血小板核糖核酸粒子的發現可以說是一個劃時代的發現。此發現對今後各種疾病的臨床治療可能起到關鍵作用。
總之，孔五一教授共發表SCI論文近30篇，總影響因子約110點，被引次數達400餘次，數年來在國際學術會議受邀報告進10次,並2次獲獎,作為第一發明人，主持申請三次發明專利，。
發表文章（全部SCI收錄）
1. P.B. Hoyer, W. Kong, E.G. Crichton, L. Beven, P.H. Krutzsch. Steroidogenic capacity and ultrastructural morphology of cultured ovine luteal cells. Biol. of Reprod. 38:909-920, 1988.
2. P.B. Hoyer and W. Kong. Protein kinase A and C activities and endogenous substrates in ovine small and large luteal cells. Mol. Cell. Endocrinol. 62:203-215, 1989.
3. S.H. Thompson, L.K. Boxhorn, W. Kong, R.E. Allen. Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulin-like growth factor I. Endocrinology 124:2110-2117, 1989.
4.W. Kong, S.L. Marion, P.B. Hoyer.Luteotropic and luteolytic responsiveness of ovine luteal cells in long-term culture. Biol. Reprod. 41:707-714, 1989.
5.W. Kong, O. Koldovsk, R.K. Rao. Appearance of exogenous epidermal growth factor in liver, bile, and intestinal lumen of suckling rats. Gastroenterology 102:661-667, 1992.
6. O. Koldovsky, W. Kong, A.F. Philipps, R.K. Rao. Studies on milk-borne insulin-like growth factor-1 and 2 (IGF-1 and IGF-2) and epidermal growth factor (EGF) in suckling rats. Endocrine Regulations 27(3):149-153, 1993.
7.W. Kong and O. Koldovsk. Uptake of intravenously administered epidermal growth factor by pancreas, submandibular gland and liver of rats: age and sex difference. Pediatric Research 36: 1994.
8.W. Kong, A.F. Philipps, O. Koldovsky. Presence of insulin-like growth factor-1 but absence of the binding proteins in the bile of rats. Am. J. Physiol, 268:R266-R271, 1995.
9. S. Bohm, W. Kong, K. Kirkwood, D.G. Payan, and N. W. Bunnett. Molecular cloning, expression and potential functions of the human proteinase-activated receptor 2. Biochem. J. 314:1009-1016, 1996.
10.W. Kong, O. Koldovsky, M. Lake, G.G. Anderson and A.F. Philipps. Organ distribution and biliary excretion of intravenously injected insulin-like growth factor-1 in suckling rats. Biology Neonate, 71(4):239-250, 1997.
11.W. Kong, K. McConalogue, L. Khitin, M. Hollenberg, D.G. Payan and S.K. Bohm, and N.W. Bunnett. Luminal trypsin may regulate enterocytes through protinase-activated receptor-2. Proc. Natl. Acad. Sci. USA. 94:8884-8889, 1997.
12.W. Kong, L. Yee, and S.J. Mulvihill. Hepatocyte growth factor (HGF) stimulates fetal gastric epithelial cell growth in vitro. J. Surg.Res. 78:161-168, 1998.
13. Bohm, S.K., McConalogue K, Kong W, and Bunnett N.W. Proteinase-activated receptors: New functions for old enzymes. News Physiol. Sci. 13:231-140, 1998.
14. Bertog, M; Letz, B; Kong, W; Steinhoff, M; Higgins, MA; Bielfeld-Ackermann, A; Frömter, E; Bunnett, NW; Korbmacher, C. Basolateral proteinase-activated receptor (PAR-2) induces chloride secretion in M-1 mouse renal cortical collecting duct cells. J. Physiol. 521 Pt 1:3-17, 1999.
15. Steinhoff, M; Corvera, CU; Thoma, MS; Kong, W; McAlpine, BE; Caughey, GH; Ansel, JC; Bunnett, NW. Proteinase-activated receptor-2 in human skin: tissue distribution and activation of keratinocytes by mast cell tryptase. Experimental Dermatology 8(4):282-94, 1999.
16.Kong, W, M.T. Longaker and H.P. Lorenz. Molecular cloning and expression of Keratinocyte Proline-Rich Protein, a novel squamous epithelial differentiation gene, isolated during skin development. J. Biol. Chem. 278 (25), 22781-22786, 2003.
17.Kong, W, M.T. Longaker and H.P. Lorenz. Cyclophilin C-associated protein is a mediator for fibronectin fragment-induced MMP-13 expression. J. Biol. Chem. 279, 55334-55340, 2004.
18. Colwell AS, Kong W, Longaker MT, and Lorenz HP: Hypertrophic scar fibroblasts have increased CTGF expression after TGF-ß stimulation. Plastic and Reconstructive Surgery. 116(5):1387-90, 2005.
19. AS. Colwell, TM. Krummel, W. Kong, Longaker MT, and Lorenz HP: Skin wounds in the MRL/MPJ mouse heal with scar. Wound Repair and Regeneration. 14:81-90, 2006.
20. W. Kong*, S. Li, C. Liu, A. S. Bari, M. T. Longaker and H. P. Lorenz. Epithelial-mesenchymal transition occurs after epidermal development in mouse skin. Experimental Cell Research. 312:3959-3968, 2006.
21.W. Kong*, S. Li, M.T. Longaker and H.P. Lorenz. Cyclophilin C-associated protein expression is up-regulated during wound healing. J. Cell. Physiol. 210:153-160, 2007.
22. Xia W, Kong W, Wang Z, Phan TT, Lim IJ, Longaker MT, Yang GP. Increased CCN2 transcription in keloid fibroblasts requires cooperativity between AP-1 and SMAD binding sites. Ann Surg. 246(5):886-95, 2007.
23.W. Kong*, S. Li, M. T. Longaker and H. P. Lorenz. Blood-derived small dot cells reduce scar in wound healing. Experimental Cell Research. 314:1529-1539, 2008.
24.W. Kong*, S. Li, M. T. Longaker and H.P. Lorenz. Cyclophilin C-associated protein/Mac-2 binding protein co-localizes with calnexin and regulates the expression of tissue transglutaminase. J Cell Phisiol. 223(1):151-7, 2010.
25. W. Kong*, Shaowei Li and H. Peter Lorenz. Germ plasm-like Dot cells maintain their wound regenerative function after in vitro expansion. Clin Exp Pharmacol Physio. 37(4):e136-44, 2010.
26. W. Kong*, Mu Nuo, Xiao Ping Zhu, Xiu Juan Han, Lihua Luo, Xian Wang. Pre-stem cell formation by non-platelet RNA-containing particle fusion. Clin Exp Pharmacol Physio. 40, 412–421, 2013.
27.W. Kong*,Mu Nuo, Xiao Ping Zhu, Xiu Juan Han, and Xian Wang. Kidney regeneration by non-platelet RNA-containing particle-derived cells. Clin Exp Pharmacol Physio. 40:724-734, 2013.
28.W. Kong*,Xiao Ping Zhu, Xiu Juan Han, Mu Nuo, and Hong Wang. Epithelial stem cells are formed by small particles released from particle-producing cells. PLOS ONE | DOI:10.1371/journal.pone.0173072 March 2, 2017.
參與書籍寫作
O. Koldovsky, W. Kong, R.K. Rao and P. Schaudies. Milk-borne peptide growth factors in human and bovine milk. Immunophysiology of the Gut. Chapter 17, Academic Press, Inc. 1993.
國際會議報告摘要
1.Hoyer, P.B. and W. Kong. Ovine luteal cells in culture: Effect of PGF2a on cell number and secretion of progesterone. Poster: 6th Biennial Ovarian Workshop, Cornell University, 1986.
2.Hoyer P.B., W. Kong and S.L. Marion. Investigation of the role of Ca++ phospholipid-dependent protein kinase in small and large ovine luteal cells. Biol. Reprod. Suppl. #1, 38:A-20, 1988.
3.Kong, W., O. Koldovsky and R.K. Rao. Absorption of rat epidermal growth factor (rEGF) from jejunum and ileum of suckling rats. Poster #622, Annual APS-SPR-APA Meeting, Anaheim, CA. Pediatr. Res. 27:108A, 1990.
4.Kong, W., O. Koldovsky and R.K. Rao. Intravenously administered I-EGF is secreted into bile and lumen of suckling rat stomach and intestine. Platform presentation #1424 at AGA Annual Meeting, San Antonio, TX. Gastroenterology 98:A-356, 1990.
5.Kong, W. and O. Koldovsky. The differences in the absorption of rat I-epidermal growth factor (rEGF) in the jejunum and ileum in suckling rats. AGA Annual Meeting, Boston, MA. Gastroenterology 104: (4) A-629, 1993.
6.Philipps, A.F., W. Kong, M. Lake and O. Koldovsky. Organ distribution and biliary secretion of intravenously (iv) injected insulin-like growth factor-1 (IGF-1) in suckling rats. Pediatric research 35:(4) 74A-429, 1994.
7.Philipps, A.F., W. Kong, M. Lake and O. Koldovsky. Presence of insulin-like growth factor-1 but absence of IGF-binding proteins in the bile of rats. AGA Annual Meeting, New Orleans, Gastroenterology 106: (4) A-627, 1994.
8.Kong, W., L. Yee, and S.J. Mulvihill. Hepatocyte growth factor (HGF) stimulates fetal gastric epithelial cell growth in vitro. AGA Annual Meeting, San Diego, CA. Gastroenterology 108: Suppl. A982, 1995.
9.Kong, W., L. Khitin, R.A. Liddle, S.R. Coughlin, D.G. Payan, K. Kirkwood, N.W. Bunnett and S.K. Bohm. Proteinase-activated receptor-2 (PAR-2) regulates prostaglandin secretion and CCK release from intestinal epithelial cells. Won best abstract award in AGA Annual Meeting, San Francisco, Gastroenterology 110: Suppl. A1089, 1996.
10.R.E. Glasgow, W. Kong and S.J. Mulvihill. Glycine-extended gastrin-17, but not gastrin-17-amide, promotes DNA synthesis in fetal rabbit gastric epithelial cells in vitro. Gastroenterology 110: Suppl. A1074, 1996.
11.K. McConalogue, W. Kong, S.K. Bohm, P.D. Gamp, O. Dery and N.W. Bunnett. Expression and cellular localization of proteinase activated receptor-2 (PAR-2) in the gastrointestinal tract. AGA annual Meeting, Washington D.C. Gastroenterology 112:4 Suppl. A1171, 1997.
12.C. Corvera, W. Kong, M. Welton and N.W. Bunnett. Proteinase-activated receptor-2 (PAR-2) is expressed by human colonic myocytes and is activated by mast cell tryptase. Acad. Surgery research annual meeting. New Orleans, 1997.
13.Kong, W., S. Bomb, L. Khitin and N.W. Bunnett. Proteinase-activated receptor-2 (PAR-2) induces immediate early genes and cyclooxygenase-2 through stimulation of MAP kinase in enterocytes. Experimental Biology Annual meeting, San Francisco, FASEB Journal 12:4-5, 892, 1998.
14.R.E. Glasgow, W. Kong, C.U. Corvera, S.J. Mulvihill and N.W. Bunnett. Expression and potential functions of proteinase activated receptor-2 (PAR-2) in pancreatic acinar cells. AGA Annual Meeting. New Orleans, Gastroenterology 114:4 PT2, G1877, 1998.
15.Kong W, Beanes SR, Benhaim P, Hedrick MH, and Lorenz HP. Expression array analysis of scarless wound repair in fetal rats. Surgical Forum 51: 548-50, 2000.
16.Ashjian P, Zuk PA, Zhu M, Kong W, Benhaim P, Lorenz HP, and Hedrick MH: Neural differentiation potential of adipose-derived mesodermal stem cells. Surgical Forum 52:523-5, 2001.
17.Lorenz HP, Kong W, Zhu M, Zhang G, Ting E, Benhaim P, Hedrick MH: MAMA is over-expressed during scarless skin wound healing and regulated by interferon-gamma. Plastic Surgery Forum 23: 34-36, November 2002.
18.Wuyi Kong, Min Zhu, Grace Chang, Kang Ting, Prosper Benhaim, Marc H. Hedrick, H. Peter Lorenz. MAMA gene expression increases during fetal skin wound healing and is regulated by interferon-gamma in fibroblasts. Oral presentation in Wound Repair and Regeneration annual meeting, Washington DC, 2002. Wound Repair and Regeneration 10:6 412, 2002.
19.Kong, W., Yun, R., Longaker, M.T., and Lorenz, H.P. Delayed cutaneous wound healing in MAMA knockout mice. Oral presentation in Wound Repair and Regeneration annual meeting in Seattle. Wound Repair and Regeneration 11:6, 513, 2003.
20.Kong, W. M.T. Longaker and H.P. Lorenz. Cyclophilin-C associated protein is involved in the regulation of MMP-1, MMP-13, MMP-3 AND MMP-14 for degradation of collagen and fibronectin. Oral presentation in 2 World union of wound healing societies meeting, Paris,France. 2004.
21.Wuyi Kong and H. Peter Lorenz. Fibronectin fragment-induced TNFa is an alternative pathway for TNFa expression in cyclophilin C-associated protein null mice. Abstract in Experimental Biology Annual meeting in San Diego. The FASEB Journal, 19:4, LB237, 2005.
22.Wuyi Kong and H. Peter Lorenz. Up-regulated p38, PCNA and decreased smooth muscle actin in Cyclophilin C-associated protein null mice during skin wound healing. Oral presentation in Wound Repair and Regeneration annual meeting, Chicago, 2005.
23.Wuyi Kong and H. Peter Lorenz. Cyclophilin C-associated protein is an intracellular mediator between tissue transglutaminase to TNFa. Oral presentation in Experimental Biology Annual meeting in San Francisco, May 2006. The FASEB Journal, 20:4, 148.12, 2006.
24.Wuyi Kong, Shaowei Li and H. Peter Lorenz. Blood-derived small size stem cells differentiate through cell fusion. Poster presentation in American society of Cell Biology Annual meeting in Washington DC, Dec 2007. J. Cell Biol,
25.Wuyi Kong, Shaowei Li, Michael T. Longaker and H. Peter Lorenz. Blood-derived Dot cells regenerate wounds by self-fusion before undergoing transdifferentiation. Poster presentation in American society of Cell Biology Annual meeting. San Francisco, Dec 2008.
26.Wuyi Kong, Shaowei Li and H. Peter Lorenz. Germ plasm-like Dot cells maintain the regenerative function after in vitro expansion. Poster presentation in American society of Cell Biology Annual meeting. San Diego, Dec 2009.
27.Wuyi Kong* and Shaowei Li. Blood-derived germ plasm-like Dot cells transform into epithelial and hair follicle stem cells during wound healing. Poster presentation in ISSCR, Toronto,Canada, June 2011.
28.Wuyi Kong*, Nuo Mu, Xiaopin Zhu, Qing Hua Cui, Li Hua Luo and Xian Wang. Human blood-derived mesenchymal stem cells are assembled. Poster presentation in ISSCR, Yokohama,Japan, June 2012.
29.Wuyi Kong*Nuo Mu, Xiaopin Zhu, Li Hua Luo and Xian Wang. Identification of non-platelet RNA-containing particles in human umbilical cord blood. Poster presentation in American society of Cell Biology Annual meeting. San Francisco, Dec 2012.
30.Wuyi Kong*, Mu Nuo, Xiao Ping Zhu, Xiu Juan Han, Xian Wang. Kidney regeneration by non-platelet RNA-containing particle-derived cells. Poster Presentation in ISSCR annual meeting, Boston,USA, June 2013.
31.Wuyi Kong*,Lichao Yang, Lei Sun, Jiang Li, XiaoPing Zhu, Mu Nuo, and Xian Wang. Brain cell regeneration by non-platelet RNA-containing particles. Poster Presentation in ISSCR annual meeting, Vancouver, Canada, June 2014.
32.Wuyi Kong*, Lei Sun, XiaoPing Zhu, Mu Nuo and Xian Wang. Non-platelet RNA-containing particles are the basic substances for assembling stem cells. Poster Presentation in ISSCR annual meeting, Stockholm, Sweden, June 2015.
33.Wuyi Kong*,Lei Sun, XiaoPing Zhu, Hong Wang,and Mu Nuo. Non-platelet RNA-containing particles are the regenerative subcellular materials. Poster Presentation in ISSCR annual meeting, San Francisco, USA, June 2016.
發明專利
專利US2015079045A1NPRCPS,PFDNCS AND USES THEROF
專利CN102876666BRNA胞體及其用途
專利US8241898B2REGENE RATIVE DOT CELLS
專利CN104884611ANPRCP, PFDNC and uses thereof
