編號 | AMS-84-10 |
拉丁菌名 | Caulobacter polymorphus nov. sp. Li, Lu et Li |
中文菌名 | 多態柄桿菌 |
定名人 | 李勤生 路景舒 黎尚豪 |
Nomencla | Li Qingsheng, Lu Jinshu, and Li Shanghao |
發表文章 | 柄桿菌與固氮 藍藻培養I. 多態柄桿菌的分離和鑑定 |
Article | Caulobacter in Nitrogen Fixing Blue-Green Algal Culture I. Isolation and Identification of Caulobacter polymorphus sp. nov. |
作者 | 李勤生 路景舒 黎尚豪 |
Author | Li Qingsheng, Lu Jinshu, and Li Shanghao |
作者單位 | 中國科學院水生生物研究所, 武漢 430071 |
摘要 | 本文報導了魚腥藻 (Anabaena spp.) 的一種異常生長現象和從魚腥藻中分離得到的柄桿菌 1017-41株的生物學特性.此菌株除具有柄桿菌特有的形態 和發育周圍期外, 還出現分叉狀和長的分節狀菌體, 與已 有記載的柄桿菌明顯不同,故定為柄桿菌屬 (Caulobacter) 的一個新種, 命名為多態柄桿菌 (Caulobacter polymorphus nov. sp.). |
Abstract | Caulobacter polymorphus sp. nov. (strain 1017-41) was isolated from a non-normalgrowth colture of Anabaena sp. 1017 strain.In this culture, concentric circular zones ofalternating yellow or dark red with bluegreen colour appeared at the surface of algalmass. In the blue-green zones, the algal filaments were healthy, but in the yellow zones,the algal filaments were colourless, more or less disrupted. Strain 1017-41 is a Gram-negative bacterium with a stalk. The cells are rod-shaped, ca. 0.5-0.8 micrometer by 2.0-6.0 micrometer, some over13.0 micrometer, generally 0.5 micrometer by 4.0 micrometer. The stalk occurs at polar or subpolar region at one end or sometime at two ends of the cell.The length of stalk is variable with culture conditions. Cell division is by means of transverse, asymmetrical fission of stalked cells.A holdfast is present at the distal end of the stalk. A lot of cells often aggregate by adhering together of the holdfasts to form achrysanthemum flower like appearance. Usually, a swarmer sibling cell occurs at the free pole of the stalked cell, and has a single polar flagellum. A remarkble feature of this strain is that short branched cell was incidently found. This cell may divide by cross septum to form three new sibling cells.Sometimes, longer cells become slender and then divide into several cells. The colonies on the 0.5% tryptone, casitone or PYE agar plate are circular, convex, smooth and orangered in colour. The pigment absorptionpeaks are vex, smooth and orange-yellow or orangered-yellow or orang-red colour. The pigment absorption peaks are at 350, 460, and 483nm. in methanol or ethanol. In liquid media, the culture is homogeneous at first, but afterward, a thin film on the surface and some precipitate in the bottom were formed.Organic nitrogen such as tryptone, peptone or casitone is needed for growth, but vitamin or biotin is not required. Glucose, starch or glycerol stimulates the growth.In the mineral medium or mineral plus glucose medium, growth is inhibited when the content of organic material is more than 0.5% in the medium. NaClis not required and SDS is an inhibitor. pH 6-7 is optimal in PYE medium, and pH range for growth is variable with medium. 30'C is well for growth.DNA G+C is 69.5 moles%. According to the morphology, development cycle, pigment, physiological and biochemical characteristics of strain 1017-41, it should be a new memberof the genus Caulobacter, and the name Caulobacter polymorphus sp. nov. is proposed. Type culture is deposited in the Institute of Hydrobiology, Academia Sinica, Wuhan. |
關鍵字 | 柄桿菌屬, 多 態柄桿菌 |
Key | |
期刊 | 微生物學報 24(2):111-116, 1984. |
Publication | Acta Microbiologica Sinica (Chinese; English Abstract), 24(2):pp.111-116, 1984. |
分享省市 | |
Place | |
採集地 | 在水生所藻類實驗室內魚腥 藻培育瓶中分離得到的柄桿菌 |
Environment | The bacterium isolated from culture bottle of the Anabaena spp., it collected from Algae Laboratory, Institute of Hydrobiology, The Chinese Academy of Scieces, Wuhan 430071, Hubei Province, China. |
海撥高度 | |
生態環境 | 實驗室內魚腥藻培育瓶 |
寄主 | 魚腥藻 |
Habitat | culture bottle of the Anabaena spp. in the Alagae Laboratory |
Host | Anabaena spp. |
採集人 | 李勤生 路景舒 黎尚豪 |
Isolation Person | Li Qingsheng, Lu Jinshu, and Li Shanghao |
採集時間 | |
保存單位 | 中國科學院水生生物研究所, 武漢 430071 |
Preservation Unit | Institute of Hydrobiology, The Chinese Academy of Scieces, Wuhan 430071, Hubei Province, China |
描述 | |
等模式 | |
Speciman No. | No.1017-41 |
全文 | |
Note | The bacterium is a impotant material, it study developing, genetics, physiology-biochemistry, ecology etc. for procaryote. |
備註 | 該菌是研究原核生物的發育, 遺傳, 生理生 化, 生態等方面的重要材料. |
