周繼勇,男,1963年生,重慶開縣人,理學博士。教授,博士生導師。農業部動物疫病病原學與免疫控制重點開放實驗室主任/動物預防醫學研究所副所長。國家傑出青年科學基金獲得者,享受國務院政府特殊津貼專家、浙江省有突出貢獻的中青年專家。現任農業部動物疫病病原學與免疫控制重點開放實驗室主任。長期致力於動物病毒性傳染病的控制理論與技術研究。主持國家傑出青年科學基金等國家及省部級科研課題30餘項。獲浙江省科技進步獎6項,一等獎1項、二等獎5項。獲國家發明專利授權6項、實用新型專利2項。獲國家二類新獸藥註冊證書1個。2006年以來在Molecular & Cellular Proteomics、Journal of Virology等國際期刊發表論文20多篇。
主要論著
1. Gu JY, Teng QY, Huang ZY, Ruan XZ, Zhou JY*. 2010. Identification of the functional Interleukin-2 binding domain of the chicken common cytokine receptor gamma chain. Developmental and comparative Immunology. 34: 258-263
2. Wu YP, Zhou JY*, Zhang X, Zheng XJ, Jiang XT, Shi LX, Yin W, Wang JH. 2009. Optimized 2-DE protocol for isolation of soluble proteins from poultry immune organs. Proteome Science. 7: 1-12
3. Wu YP, Hong LL, Ye JX, Huang ZY, Zhou JY*. 2009. The VP5 protein of infectious bursal disease virus promotes virion release from infected cells and is not involve in cell death. Archives of Virology. 154(12):1873-1882
4. Zhang X, Zhou JY*, Wu YP, Zheng XJ, Ma GP, Wang ZT, Jin YL, He JL, Yan Y. 2009. Differential Proteome Analysis of host cells infected with Porcine Circovirus Type 2. Journal of Proteome Research. 8: 5111-5119
5. Shen HG, Zhou JY*, Zhang X, Huang ZY, He JL, Yan Y. 2009. Interference of porcine circovirus type 2 ORF2 immunogenicity by ORF1 and ORF3 mixed DNA immunizations in mice. Virology. 393: 104-111
6. Zhou JY*, Sun WB, Wang JH, Guo JQ, Yin W, Wu NP, Li LJ, Yan Y, Liao M, Huang Y, Luo KJ, Jiang XT, 7. Chen HL. 2009. Characterization of the H5N1 Highly Pathogenic Avian Influenza Virus derived from Wild Pikas in China. Journal of Virology. 83(17): 8957-8964.
8. Shi LX, Li HB, Ma GP, Zhou JY*, Hong LL, Zheng XJ, Wu YP, Wang YZ, Yan Y. 2009. Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts. Virus Genes. 39: 46-52
9. Wang YZ, Zhou JY*, Wu XP, Li HB, Wu YP, Shi LX, Yan Y. 2009. Antibody to VP4 protein is an indicator discriminating pathogenic and nonpathogenic IBDV infection. Molecular Immunology. 46:1964-1969
10. Shang SB , Jin YL, Jiang XT, Zhou JY*, Zhang X, Xing G, Yan Y. 2009. Fine Mapping of Antigenic Epitopes on Capsid Proteins of Porcine Circovirus, and Antigenic Phenotype of Porcine Circovirus Type 2. Molecular Immunology. 46:327-334
11. Zhang X, Ma GP, Li YF, Jiang XT, He JL, Zhou JY*. 2009. Characterization of Monoclonal Antibody against Replication-associated protein of Porcine Circovirus. DNA and Cell Biology. 28(1):23-29
12. Shen HG, Zhou JY*, Huang ZY, Guo JQ, Xing G, He JL, Yan Y, Gong LY. 2008. Protective immunity against porcine circovirus 2 by vaccination with ORF2-based DNA and subunit vaccines in mice. Journal of General Virology. 89 (8): 1857-1865.
13. Zheng XJ, Hong LL, Shi LX, Guo JQ, Sun Z, Zhou JY*. 2008. Proteomics Analysis of Host Cells Infected with Infectious Bursal Disease Virus. Molecular & Cellular Proteomics. 7: 612-615
14. Shang SB, Li YF, Guo JQ, Wang ZT, Chen QX, Shen HG, Zhou JY*. 2008. Development and validation of a recombinant capsid protein-based ELISAfor detection of antibody to porcine circovirus type 2. Research in Veterinary Science. 84: 150-157
15. Wang JY, Fang J, Guo JQ, Teng QY, Huang ZY, Gu JY, Shen HG, Zhou JY*. 2007. Molecular Cloning and Characterization of Duck CD25. Veterinary Immunology and Immunopathology. 117: 266-274
16. Hu JQ, Li YF, Guo JQ, Shen HG, Zhang DY, Zhou JY*. 2007. Production and Characterization of Monoclonal Antibodies to poly100S1 Protein of Avian Infectious Bronchitis Virus. Zoonoses and Public Health. 54: 69-7
17. Ye JX, Chen QX, Zhou JY*, Li LY. 2007. Cloned vero cell lines transfected with full-length A-segment or ORF1 cDNA sequence of IBDV. Cell Biology International. 31: 165-172
18. Zheng XJ, Hong LL, Li YF, Guo JQ, Zhang GP, Zhou JY*. 2006. In vitro Expression and Monoclonal antibody of RNA-dependent RNA polymerase for Infectious Bursal Disease Virus. DNA and Cell Biology. 25(11): 646-654
19. Wang JY, Qi J, Shen HG, Zhou JY*, Fang J, Chen HX, Wang ZP, Li HL. 2006. In vivo CD4+ T-cell up-regulation and high dose side effects of refolded duck interleukin-2. Cytokine. 34: 297-302
20. Teng QY, Zhou JY*, Wu JJ, Guo JQ, Shen HG. 2006. Characterization of chicken interleukin 2 receptor alpha chain, a homolog to mammalian CD25. FEBS Letters 580 (17): 4274-4281
21. Zhou JY*, Shen HG, Chen HX, Tong GZ, Liao M, Yang HC, Liu JX.2006. Characterization of a highly pathogenic H5N1 influenza virus derived from bar-headed geese in China. Journal of General Virology. 87: 1823-1833
22. Chen HX, Shen HG, Li XL, Zhou JY*, Hou YQ, Guo JQ, Hu JQ. 2006. Seroprevalance and identification of influenza A virus infection from migratory wild waterfowl in China (2004-2005). Journal of Vterinary Medicine Series B. 53 (4): 166-170
Shi LX, Li HB, Ma GP, Zhou JY*, Hong LL, Zheng XJ, Wu YP, Wang YZ, Yan Y. 2008. Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts. Virus Genes.(in press)
Shang SB , Jin YL, Jiang XT, Zhou JY*, Zhang X, Xing G, Yan Y. 2008. Fine Mapping of Antigenic Epitopes on Capsid Proteins of Porcine Circovirus, and Antigenic Phenotype of Porcine Circovirus Type 2. Molecular Immunology. 46:327-334
Zhang X, Ma GP, Li YF, Jiang XT, He JL, Zhou JY*. 2008. Characterization of Monoclonal Antibody against Replication-associated protein of Porcine Circovirus. DNA and Cell Biology. 27(in press)
Shen HG, Zhou JY*, Huang ZY, Guo JQ, Xing G, He JL, Yan Y, Gong LY. 2008. Protective immunity against porcine circovirus 2 by vaccination with ORF2-based DNA and subunit vaccines in mice. Journal of General Virology. 89 (8): 1857 – 1865.
Zheng XJ, Hong LL, Shi LX, Guo JQ, Sun Z, Zhou JY*. 2008. Proteomic Analysis of Host Cells Infected with Infectious Bursal Disease Virus. Molecular & Cellular Proteomics 7: 612-615(IF 9.62)
Yue WF, Li XH, Wu WC, Roy B, Li GL, Liu JM, Wu XF, Zhou JY, Zhang CX, David WCC , Miao YG. 2008. Improvement of recombinant baculovirus infection efficiency to express manganese superoxide dismutase in silkworm larvae through dual promoters of Pph and Pp10. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 78(4): 651-657
Shang SB, Li YF, Guo JQ, Wang ZT, Chen QX, Shen HG, Zhou JY*. 2008. Development and validation of a recombinant capsid protein-based ELISA for detection of antibody to porcine circovirus type 2. Research in Veterinary Science. 84: 150-157
Wu JS, Liu YH, Hu SH, Zhou JY*. 2008. Development of a rapid PCR test for identification of Streptococcus agalactiae in milk samples collected on filter paper disks. Asian-Australasian Journal of Animal Sciences (Asian-Aust. J. Anim. Sci.) 21(1): 124-130
Yue WF, Liu JM, Sun JT, Li GL, Li XH, Wu XF, Sun HX, Zhou JY, Miao YG. 2007. Immunity Promotion and Proteomic Identification in Mice upon Exposure to Manganese Superoxide Dismutase Expressed in Silkworm Larvae. Journal of Proteome research. 6(5): 1875-1881
Wu JX, Yu L, Li L, Hu JQ, Zhou JY Zhou XP. 2007. Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens. Plant Biotechnology Journal. 5:570-578
Wu JS, Liu YH, Hu SH, Zhou JY*. 2007. Determination of lactose on filter paper perserved milk samples stored at different temperatures and for different periods: comparison with tube sample. Milk Science International (ISSN0026-3788). 62(4): 398-401
Chen QX, Ye JX, Zhou JY*, Chen TF, Hui-gang Shen HG, Shang SB. 2007. Serological survey of serum against porcine circovirus type 2 in swine, chicken, duck, cattle and goat from the Zhejiang province, China. Revue De Medecine Veterinaire 158(8-9): 458-462
Wang JY, Fang J, Guo JQ, Teng QY, Huang ZY, Gu JY, Shen HG, Zhou JY*. 2007. Molecular Cloning and Characterization of Duck CD25. Veterinary Immunology and Immunopathology. 117: 266-274
Hu JQ, Li YF, Guo JQ, Shen HG, Zhang DY and Zhou JY*. 2007. Production and Characterization of Monoclonal Antibodies to poly100S1 Protein of Avian Infectious Bronchitis Virus. Zoonoses and Public Health. 54: 69-77
Ye JX, Chen QX, Zhou JY*, Li LY. 2007. Cloned vero cell lines transfected with full-length A-segment or ORF1 cDNA sequence of IBDV. Cell Biology International 31: 165-172
Zheng XJ, Hong LL, Li YF, Guo JQ, Zhang GP, Zhou JY*. 2006. In vitro Expression and Monoclonal antibody of RNA-dependent RNA polymerase for Infectious Bursal Disease Virus. DNA and Cell Biology. 25(11):646-654
Wang JY, Qi J, Shen HG, Zhou JY*, Fang J, Chen HX, Wang ZP, Li HL. 2006. In vivo CD4+ T-cell up-regulation and high dose side effects of refolded duck interleukin-2. Cytokine. 34: 297-302
Teng QY, Zhou JY*, Wu JJ, Guo JQ, Shen HG. 2006. Characterization of chicken interleukin 2 receptor alpha chain, a homolog to mammalian CD25. FEBS Letters 580 (17): 4274-4281
Zhou JY*, Shen HG, Chen HX, Tong GZ, Liao M, Yang HC, Liu JX.2006. Characterization of a highly pathogenic H5N1 influenza virus derived from bar-headed geese in China Journal of General Virology. 87: 1823-1833
Guo JQ, Li QM, Zhou JY*, Zhang GP, Yang YY, Xing GX, Zhao D, You SY, Zhang CY. 2006. Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system. 2006. Protein Expression and Purification. 45 (1): 168-174
Chen HX, Shen HG, Li XL, Zhou JY*, Hou YQ, Guo JQ, Hu JQ. 2006. Seroprevalance and identification of influenza A virus infection from migratory wild waterfowl in China (2004-2005). Journal of Vterinary Medicine Series B. 53 (4): 166-170
Zhang GP, Guo JQ, Zhou JY*, Wang XN, Li QM, Yang YY, Shen HG, Zhao D, Zhang H, Xi J, Wang L, Qiao SL, Jin X. 2006. Identification of the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFc gamma 2R) using synthetic peptides. FEBS Letters 580 (5): 1383-1390
Guo JQ, Li QM, Zhou JY*, Zhang GP, Yang YY, Xing GX, Zhao D, You SY, Zhang CY. 2006. Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system. Protein Expression and Purification. 45:168-174
Zhou JY*, Chen QX, Ye JX, Shen HG, Chen TF, Shang BS. 2006. Serologic investigation and genomic characterization of PCV2 isolates from different geographic regions of Zhejiang province in China. Veterinary Research Commications. 30(2):205-220
Zhang DY,Zhou JY*, Fang J, Hu JQ, Wu JX, An-Xiong Mu A. 2005. An ELISA for Antibodies to Infectious Bronchitis Virus based on Nucleocapsid Protein Produced in Escherichia coli Veterinarni Medica 50(8): 336-344
Wang XN, Zhang GP, Zhou JY*, Feng CH, Yang YY, Li QM, Guo JQ, Qiao HX, Xi J, Zhao D, Xing GX, Wang ZL, Wang SH, Xiao ZJ, Li XW, Deng RG. 2005. Identification of neutralizing epitopes on the VP2 protein of infectious bursal disease virus by phage displayed heptapeptide library screening and synthetic peptide mapping. Viral Immunol. 18(3): 549-557
Zhou JY*, Chen JY, Wang JY, Wu JX, Gong H, and Chen QJ. cDNA cloning and functional analysis of goose interleukin-2. 2005. Cytokine 30(6): 328-338
Zhou JY*, Shang SB, Gong H, Chen QX, Wu JX, Shen HG, Chen TF, Guo JQ. 2005. In vitro expression, Monoclonal Antibody and Bioactivity for Capsid Protein of Porcine Circovirus Type II without Nuclear Localization Signal. Journal of Biotechnology. 118(2):201-211
Zhou JY*, Ye JX, Zheng XJ, Guo JQ. 2005. Characterization of subtype isolates of infectious bursal disease virus in China from chicken layers. Progress in Biochemistry and Biophysics. 32(1):37-45
Zhou JY*, Wang JY, Chen JG, Wu JX, Gong H, Teng QY, Guo JQ, Shen HG. 2005. Cloning, in vitro expression and bioactivity of duck interleukin-2. Molecular Immunology 42(5):589-598
Guo JQ, Chen L, Ai HW, Jing JN, Zhou JY*, Zhang CY, You SY. 2004. A Novel Fusion Protein of IP10-scFv Retains Antibody Specificity and Chemokine Function. Biochemical and Biophysical Research Communications. 320: 506-513
Zhou JY*, Zhang DY, Ye JX, Cheng LQ. 2004. Characterization of an Avian Infectious Bronchitis Virus Isolated in China from Chickens with Nephritis. Journal of Veterinary medicine series B-Infectious diseases and Veterinary Public Health. 51:147-152
Zhou JY*, Cheng LQ, Zheng XJ, Wu JX, Shang SB, Wang JY, Chen JG. 2004. Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus. Journal of Biotechnology. 111:121-130
Zhou JY, Wu JX, Cheng LQ, Zheng XJ, Gong H, Shang SB, Zhou EM. 2003. Expression of Immunogenic S1 Glycoprotein of Infectious Bronchitis Virus in Transgenic Potatoes. Journal of Virology,77(16):9090-9093
Zhou JY*, Chen JG, Wang JY, Kwang J. 2003. Cloning and Genetic Evolution Analysis of chIL-2 Gene of Chinese Local Breeds. Progress in Biochemistry and Biophysics. 30 (3): 384-389
Wu JX, Zhou JY*, Zhou XP. 2003. Transgenic potato containing immunogenic gene of avian coronavirus and its immunogenicity in mice ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35 (11): 1011-1015
Zhou JY, Ding HM, Chen LQ and Kwang J. Variation of Nucleocapsid Protein Gene of Infectious Bronchitis Virus and its Expression in Escherichia coli. J.Zhejiang Univ.(Agri & Life ed). 2003,29(1):1-9.(PDF)