劉玉煥(中山大學生命科學學院教授)

劉玉煥(中山大學生命科學學院教授)

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劉玉煥，男，1987年生，博士，中山大學生命科學學院教授、博士生導師，中國微生物學會環境微生物學專業委員會委員。

基本介紹

  • 中文名：劉玉煥
  • 出生地：1987年
  • 學位/學歷：博士
  • 專業方向：環境微生物與蛋白質工程
  • 任職院校：中山大學
研究方向,人物經歷,講授課程,學術成就,承擔課題,論文專著,發明專利,獲獎情況,

研究方向

環境微生物與蛋白質工程：主要研究興趣在以下3個方面：（1）深度挖掘極端微生物中獨特的基因資源，研究具有優良性能蛋白質的結構與功能之間的關係；（2）系統研究微生物對外源蛋白質分泌表達的作用機理；（3）採用合成生物學與代謝工程構建高效降解有毒有害污染物的超級工程菌，解決日益嚴重的環境污染問題，達到資源化、減量化和增值化的目的。

人物經歷

2007/01 –至今，中山大學，生命科學學院，教授
2002/01 –2006/12，中山大學，生命科學學院，副教授
1996/01 –2001/12，中山大學，生命科學學院，講師
1991/01 –1995/12，中山大學，生命科學學院，助教

講授課程

微生物學與微生物學實驗，微生物生物技術，神奇的微生物

學術成就

在國際生物工程領域權威期刊Biotechnol.Biofuels. Bioresour.Technol.Microbial Cell. Factor、J. Agric. Food Chem.等雜誌上發表多篇代表作，已發表SCI論文39篇，獲得授權發明專利7項，2016與2015年分別獲得廣東省和廣州市科技進步二等獎各一次。

承擔課題

近5年，主持了國家自然科學基金“β-葡萄糖苷酶耐受葡萄糖的分子機制”， “紅樹林環境宏基因組文庫新型阿魏酸酯酶的篩選及結構功能的解析”、國家海洋公益性行業科研專項 “固定化β-半乳糖苷酶研發的關鍵技術集成與示範、廣東省科技重大專項“南海海洋微生物活性物質的研究與利用”及廣東省海洋漁業科技推廣專項“新型海洋高效轉糖基化β-半乳糖苷酶的研究與利用”。

論文專著

  1. Cao Li‑chuang,Liu Yu-huan, Xie Wei.*, and Wang Z.(*)Structures of a glucose-tolerant β-glucosidase provide insights into its mechanism.Journal of Structural Biology.2017,198 (3): 154-162.
  2. Xiaolong Liu, Mingjun Liang 1 Yuhuan Liu and Xinjiong Fan*. Directed evolution andsecretory expression ofa pyrethroid-hydrolyzing esterase withenhanced catalytic activity andthermostability.Microb Cell Fact. 2017. 16:81
  3. Guang-hui Ren, Li-chuang Cao, Wei Kong, Zhi-jun Wang, and Yu-huan Liu* . Efficient Secretion of the β‑Galactosidase Bgal1‑3 via both Tat- Dependent and Tat-Independent Pathways in Bacillus subtilis.J. Agric. Food Chem.2016, 64, 5708−5716
  4. Xiwen Liu, Li-chuang Cao, Xin-jiong Fan, Yu-huan Liu* & Wei Xie*. Engineering of a thermostable esterase Est816 to improve its quorum- quenching activity and the underlying structural basis.Scientific Reports.2016, 6:38137
  5. Lichuang Cao, Zhijun Wang, Guang‑hui Ren, Wei Kong, Liang Li, Wei Xie andYuhuan Liu*.Engineering a novel glucose-tolerant β- glucosidase assupplementation toenhance the hydrolysis ofsugarcane bagasse athigh glucose concentration.Biotechnol. Biofuels.2015, 8:202. 2015.
  6. Qingnan Tian, Caiyan Wang,Yuhuan Liuand Wei Xi*. Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase.Nucleic Acids Research.2015, 43: 5, 2015
  7. Li-chuang Cao#, Ran Chen#, Wei Xie*, andYuhuan Liu*. Enhancing the Thermostability of Feruloyl Esterase EstF27 by Directed Evolution and the Underlying Structural Basis.J. Agric. Food Chem.2015, 63, 8225−8233.
  8. Liang Li#, Gang Li#, Li-chuang Cao, Guang-hui Ren, Wei Kong, Si-di Wang, Geng-shan Guo,andYuHuan Liu*. Characterization of the Cross-Linked Enzyme Aggregates of a Novel β-Galactosidase, a Potential Catalyst for the Synthesis of Galacto-Oligosaccharides.J. Agric. Food Chem.2015, 63, 894−901.
  9. Jian Yao, Geng Shan Guo, Guang Hui Ren,Yuhuan Liu*.Production, characterization and applications of tannase.J. Mol. Catal. B: Enzym.2014, 101: 137– 147.
  10. Si-di Wang, Geng-shan Guo, Liang Li, Li-chuang Cao, Ling Tong,Guang-hui Ren,Yuhuan Liu*.Identification and characterization of an unusualglycosyltransferase-like enzyme withβ-galactosidaseactivity from a soil metagenomic library.Enzyme and Microbial Technology,2014, 57: 26–35.
  11. Jian Yao, Qinglong Chen, Guoxiang Zhong, Wen Cao, An Yu andYuhuan Liu*.Immobilization and Characterization of Tannase from a MetagenomicLibrary and Its Use for Removal of Tannins from Green Tea InfusionJ. Microbiol. Biotechnol.2014, 24(1): 80–86.
  12. Jian Yao, Qing Long Chen, Ai Xi Shen, Wen Cao,Yuhuan Liu*.A novel feruloyl esterase from a soil metagenomic library with tannase activity. J. Mol. Catal. B: Enzym.2013, 95: 55– 61.
  13. Sidi Wang, Kui Wang, Liang Li ,Yuhuan Liu*.Isolation and characterization of a novel organic solvent-tolerant and halotolerant esterase from a soil metagenomic library.J. Mol. Catal. B: Enzym.2013, 95: 1– 8.
  14. Xia Zhang#, He Li#, Chang-Jie Li, Teng Ma, Gang Li*.andYuhuan Liu*. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples ofTurpanBasin.BMC Microbiol.2013, 13:237.
  15. Gang Li, Yang Jiang, Xin-jiong Fan,Yuhuan Liu*.Molecular cloning and characterization of a novel b-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil metagenomic library.Bioresource Technol.2012, 123; 15–22.
  16. Xinjiong Fan, Xiaolong Liu,Yuhuan Liu*.The cloning and characterization of one novel metagenome-derivedthermostableesterase acting onN-acylhomoserine lactones.J. Mol. Catal. B: Enzym.2012, 83:29-37.
  17. Xinjiong Fan, Xiaolong Liu, Rui Huang andYuhuan Liu*.Identification and characterization of a novel thermostable pyrethroid-hydrolyzing enzyme isolated through metagenomic approach.Microbial Cell Factories.2012, 11:33.
  18. Shi-Qin Yu,Yuhuan Liu*.Expression and one-step purification ofβ-galactosidase by fusion with elastin-like polypetides.Process Biochem. 2012,47: 1108–1114,
  19. Kui Wang, Yi Lu, Wei Qu Liang, Si Di Wang, Yang Jiang, Rui Huang, and Yu Huan Liu*. Enzymatic Synthesis of Galactooligosaccharides in an Organic−Aqueous Biphasic System by a Novel β-Galactosidase from a Metagenomic Library.J. Agric. Food Chem.2012,60, 3940−3946,
  20. Shangke Chen, Kui Wang,Yuhuan Liu*and Xiaopeng Hu*. Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library.Acta Crystallographica Section F Structural Biology and Crystallization Communications (Acta Cryst. ).2012, F68:767-770.
  21. Jian Yao, Xin Jiong Fan, Yi Lu,Yuhuan Liu*. Isolation and Characterization of a Novel Tannase from a Metagenomic Library.J. Agric. Food Chem.2011,59: 3812–3818.
  22. Xinjiong Fan, Xiaolong Liu, Kui Wang, Sidi Wang, Rui Huang,Yuhuan Liu*.Highly soluble expression and molecular characterization of an organic solvent-stable and thermotolerant lipase originating from the met genome.J. Mol. Catal. B: Enzym.2011, 72: 319– 326.
  23. Shu Li Sang, Gang Li, Xiao Peng Hu,Yuhuan Liu*.Molecular Cloning, Overexpression and Characterization of a Novel Feruloyl Esterase from a Soil Metagenomic Library.J. Mol. Microbiol. Biotechnol. 2011, 20:196–203.
  24. Liping Zhang, Kui Wang,ZhongxingMo,Yuhuan Liu*and Xiaopeng Hu*.Crystallization and preliminary X-ray analysis of a cold-activeβ- galactosidase from the psychrotrophic and halotolerantPlanococcussp. L4.Acta Crystallographica Section F Structural Biology and Crystallization Communications (Acta Cryst. ).2011, F67: 911–913.
  25. Yuhuan Liu, Mao Ye, Yi Lu, Xia Zhang and Gang Li. Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution.Appl. Microbiol .Biotechnol.2011, 91:667–675.
  26. Ye M,Li G, Liang WQ,Y.H Liu*.Molecular cloning and characterization of a novelmetagenome-derivedmulticopper oxidase with alkalinelaccase activityand highly soluble expression.Appl.Microbiol.Biotechnol.2010, 87:1023-1031.
  27. Kui Wang, Gang Li, Shi Qin Yu, Chen Ting Zhang,Yuhuan Liu*. A novel metagenome-derivedβ-galactosidase: gene cloning, overexpression, purification and characterization.Appl. Microbiol. Biotechnol.2010, 88:155–165.
  28. Gang Li, Kui Wang,YuhuanLiu*.Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the Metagenome.Microbial Cell Factories.2008, 7:38.
  29. Lixiang Cao, Min Jiang, Zhirui Zeng, Aixue Du, Hongming Tan,Yuhuan Liu*.Trichoderma atroviride F6 improves phytoextraction efficiency of mustard (Brassica juncea (L.) Coss. var. foliosa Bailey) in Cd, Ni contaminated soils.Chemosphere. 2008, 71: 1769–1773
  30. Ji M. Hu,He. Li, Li X. Cao, Pei C.Wu,Chen T. Zhang, Shu L.Sang,Xiao Y. Zhang, Min J.Chen, Jia Q. Lu,YuhuanLiu*.Molecular cloning and characterization of the gene encoding cold-active β-galactosidase from apsychrotrophic and halotolerantPlanococcussp. L4.J. Agric. Food Chem.2007, 55:2217-2224.
  31. Li HUAPING, Lian JIE, Wang ZHIFENG, Zhong YINGCHANG, and Liu Yuhuan*. Cloning and Functional Expression of Ubiquitin-Like Protein Specific Proteases Genes from Candida.Biol. Pharm. Bull. 2007, 30(10): 1851—1855.
32.He Li,YuhuanLiu*,Na Luo, Xiao Y. Zhang, Tian G. Luan, Ji M. Hu,Zhuo Y. Wang, Pei C. Wu, Min J. Chen, Jia Q. Lu.Biodegradation of benzene and its derivatives by apsychrotolerantand moderately haloalkaliphilicPlanococcussp. strain ZD22.Research in Microbiology. 2006, 157:629-636.
33. H. Li, W.Q. Liang, Z.Y. Wang, N. Luo, X.Y. Wu, J.M. Hu, J.Q. Lu, X.Y. Zhang, P.C. Wu andYuhuan Liu*,Enhanced production and partial characterization of thermostable α-galactosidase by thermotolerantAbsidiasp.WL511.World Journal of Microbiology & Biotechnology,2006, 22:1–7.
34. Pei C. Wu,YuhuanLiu*, Zhuo Y. Wang,He Li, Wei Q. Liang,Na Luo, Ji M. Hu, Xiao Y. Zhang, Jia Q. Lu, Tian G. Luan and Li X. Cao. Molecular cloning, Purification and Biochemical Characterization of A Novel pyrethroid-hydrolyzing esterase FromKlebsiellasp. strainZD112.J. Agric. Food Chem.2006, 54: 836-842.
35. W.Q. Liang, Z.Y. Wang, He Li, P. C. WU, J. M. HU, N. LUO, L. X. CAO, andYuhuanLiu*.Purification and Characterization of a novel pyrethroid hydrolase from AspergillusnigerZD11.J. Agric. Food Chem..2005, 53: 7415-7420.
36.Liu Yang, Zhou Shining, Chen Zhishi, Zhong Yingchang, Liu Yuhuan*. 2004. Purification and Characterization of an Isoflavone Conjugates-Hydrolyzing β-Glucosidase from Endophytic Bacterium.J. Agric. Food Chem.2004, 52: 1940—1944.
37.YuhuanLiu*, Liu yang, Chen zhi-shi, Lian jie, Huang xiao, and Chung ying-cheng. Purification and characterization of a novel organophosphorus pesticide hydrolase from Penicillium lilacinum BP303.Enzyme and Microbial Technology. 2004, 34:297-303.
38. Zhang Qing, Liu Yang,YuhuanLiu*. Purification and characterization of a novel carbaryl hydrolase from AspergillusnigerPY168.FEMS Microbiology Letters,2003, 228:39-44.
39.YuhuanLiu*, Cheng, Y C., Xiong Y. Purification and characterization of Dimethoate-degrading enzyme of AspergillusnigerZHY-256 isolatedfrom sewage. 2001,Applied and Environmental Microbiology. 2001, 67: 3746-3749.

發明專利

  1. 劉玉煥，李剛，王魁。DNA序列在乳品業領域中的套用，2011.12，中國，ZL200910214209.7
  2. 劉玉煥，李剛，余世琴。一種利用類彈性蛋白標籤純化乳糖酶的方法,2012.5，中國，ZL201010244640.9
  3. 劉玉煥，梁衛驅,劉孝龍，羅華建，范新炯，胡珊，羅詩，劉遠星。一種新型酯酶及其套用，2012.6，中國，ZL201010547206.8
  4. 劉玉煥，劉孝龍，王魁，范新炯，魯毅,一種具有高效轉糖基β-半乳糖苷酶的新基因及套用技術.2012.12，中國，ZL201110001568.1
  5. 劉玉煥，范新炯。一種新型酯酶及其套用。2013.9，中國，授權號：ZL201210196902.8
  6. 劉玉煥，汪思迪，李良，曹立創，童鈴，郭耿珊。一種糖基轉移酶的新基因及套用。2014.9，中國，專利授權號：ZL201310149841.4
  7. 劉玉煥，童鈴，曹立創，李良，郭耿珊，汪思迪。一種高效水解大豆異黃酮苷的β-葡萄糖苷基因與套用。2015.8，中國，專利授權號：ZL201310149771.2

獲獎情況

  1. 生物酶製劑在液體洗滌劑中的結構研究與套用，2016年廣東省科技進步二等獎（第二完成人）
  2. 生物酶製劑在液體洗滌劑中的結構研究與套用，2015年廣州市科技進步二等獎（第二完成人）
  3. 菊酯類農藥降解酶高效表達工程菌的構建及發酵條件最佳化，2014年東莞市科學技術進步三等獎（第三完成人）

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