曹立創

曹立創，理學博士，現為中山大學生命科學學院副教授、碩士生導師。

基本介紹

• 中文名：曹立創
• 學位/學歷：博士
• 職業：教師

人物經歷

2022/10- 中山大學，生命科學學院，副教授

2017/07-2022/08，中山大學，生命科學學院，博士後，合作導師：李文均

2012/09–2017/06，中山大學，生命科學學院，微生物學，理學博士，導師：劉玉煥

2008/09–2012/06，廣東工業大學，環境科學與工程學院，生物工程，工學學士

研究方向

酶工程與合成生物學：（1）極端環境酶資源挖掘；（2）酶工程、酶的結構與功能關係；（3）高性能底盤細胞構建。

論文

14. Shuifeng Li#, Lichuang Cao#, Xiangpeng Yang, Xiangrui Wu,Shujing Xu and Yuhuan Liu. Simultaneously optimizing multiple properties ofβ-glucosidase Bgl6 using combined (semi-)rational design strategies andinvestigation of the underlying mechanisms. Bioresour. Technol. 374, 128792, 2023.

13. Lichuang Cao#, Ran Chen#, Xin Huang,Shuifeng Li, Sufang Zhang, Xiangpeng Yang, Zongmin Qin, Wei Kong, Wei Xie* andYuhuan Liu*. Engineering of β-glucosidase Bgl15 with simultaneously enhancedglucose tolerance and thermostability to improve its performance in high-solidcellulose hydrolysis. J. Agric. Food Chem. 68, 5391-5401, 2020.

12. Zongmin Qin, Shuifeng Li, Xin Huang, Wei Kong,Xiangpeng Yang, Sufang Zhang, Lichuang Cao* and Yuhuan Liu*.Improving galactooligosaccharide synthesis efficiency of β-galactosidaseBgal1-3 by reshaping the active site with an intelligent hydrophobic amino acidscanning. J. Agric. Food Chem. 67, 11158-11166, 2019.

11. Xiwen Liu#, Lichuang Cao#, Jianhua Zeng, Yuhuan Liu*, WeiXie*. Improving the cellobiose-hydrolysis activity and glucose-tolerance of athermostable β-glucosidase through rational design. Int. J. Biol. Macromol.136, 1052-1059, 2019.

10. Lichuang Cao, Shuifeng Li, Xin Huang, Zongmin Qin, WeiKong, Wei Xie*, Yuhuan Liu*. Enhancing the thermostability of highly active andglucose-tolerant β-glucosidase Ks5A7 by directed evolution for good performanceof three properties. J. Agric. Food Chem., 66(50), 13228-13235, 2018.

9. Xin Huang#, Lichuang Cao#, Zongmin Qin, Shuifeng Li, WeiKong, Yuhuan Liu. Tat-independent secretion of polyethylene terephthalatehydrolase PETase in Bacillus subtilis 168 mediated by its native signalpeptide. J. Agric. Food Chem., 66(50), 13217-13227, 2018.

8. Lichuang Cao#, Guanghui Ren#, Zongmin Qin, Xin Huang, WeiKong, Zhijun Wang, Weiqu Liang, Xiaogang Bi*, Yuhuan Liu*. Improving thesecretion yield of the β-galactosidase Bgal1-3 in Pichia pastoris foruse as a potential catalyst in the production of prebiotic-enriched milk. J.Agric. Food Chem., 65(49), 10757-10766, 2017.

7. Panjiao Pang, Lichuang Cao, Yuhuan Liu, Wei Xie*, ZhongWang*. Structures of a glucose-tolerant β-glucosidase provide insights into itsmechanism. J. Struct. Biol., 198(3):154, 2017.

6. Xiwen Liu#, Lichuang Cao#, Xin-jiong Fan, Yuhuan Liu*, WeiXie*. Engineering of a thermostable esterase Est816 to improve itsquorum-quenching activity and the underlying structural basis. Sci. Rep.,6:38137, 2016.

5. Guang‑hui Ren#, Lichuang Cao#, Wei Kong, Zhi‑jun Wang,Yuhuan Liu. Efficient secretion of β-galactosidase Bgal1-3 via bothTat-dependent and Tat-independent pathways in Bacillus subtilis. J.Agric. Food Chem. 64(28): 5708-5716, 2016.

4. Lichuang Cao, Zhi‑jun Wang, Guang‑hui Ren, WeiKong, Liang Li, Wei Xie and Yuhuan Liu. Engineering a novel glucose-tolerantβ-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasseat high glucose concentration. Biotechnol. Biofuels. 8:202. 2015.

3. Lichuang Cao#, Ran Chen#, Wei Xie*, and Yuhuan Liu*.Enhancing the thermostability of feruloyl esterase EstF27 by directed evolutionand the underlying structural basis. J. Agric. Food Chem. 63, 8225−8233. 2015.

2. Liang Li#, Gang Li#, Lichuang Cao, Guang-hui Ren, WeiKong, Si-di Wang, Geng-shan Guo, and Yuhuan Liu*. Characterization of thecross-linked enzyme aggregates of a novel β-galactosidase, a potential catalystfor the synthesis of galacto-oligosaccharides. J. Agric. Food Chem. 63,894-901. 2015.

1. Si-di Wang, Geng-shan Guo, Liang Li, Lichuang Cao, LingTong, Guang-hui Ren, Yuhuan Liu*. Identification and characterization of anunusual glycosyltransferase-like enzyme with β-galactosidase activity from asoil metagenomic library. Enzyme Microb. Technol, 57: 26-35, 2014.

專利

3. 劉玉煥，曹立創，張素芳，黃磊，楊向鵬，衛雅萱。熱穩定性提高的β-半乳糖苷酶突變體及其套用，2023-06-19，中國, 202310731755.8。

2. 劉玉煥，曹立創，李水鳳，黃欣，覃宗敏，孔偉。高熱穩定性β-葡萄糖苷酶突變體及其套用，2018-11-21，中國, ZL201811393399.9。

1. 劉玉煥，曹立創，黃欣，李水鳳，覃宗敏，孔偉。熱穩定性提高的β-葡萄糖苷酶突變體及其套用，2018-11-21，中國, ZL201811393398.4。