光壽紅，男，博士，中國科學技術大學博士生導師。
基本介紹
- 中文名：光壽紅
- 畢業院校：美國威斯康星大學癌症研究所
- 學位/學歷：博士
- 專業方向：真核細胞中RNA的表達與加工的調節
- 任職院校：中國科學技術大學
人物經歷,研究方向,科研成果,論文專著,
人物經歷
1996年在中國科學技術大學生物系獲得學士學位，
1996-1999年師從中國科大施蘊渝院士進行多肽的溶液構象研究，並獲得碩士學位。
1999-2004年，在美國威斯康星大學癌症研究所師從Janet Mertz教授，研究真核生物中病毒起源的無內含子RNA的表達和調控機制，並獲得博士學位。
2005-2010年，作為博士後在威斯康星大學遺傳系Scott Kennedy助理教授實驗室，從事模式生物中小干擾RNA的功能和調控機理的研究。
研究方向
1、真核細胞中RNA的表達與加工的調節
2、真核生物中轉錄調節機制
3、模式生物中非編碼RNA的表達與調節機制
科研成果
發現真核生物中無內含子的基因含有一些順式作用的RNA序列元素，它們可以和細胞內的特定蛋白相互結合，來提高該無內含子基因宙擊辣的前mRNA的穩定性，poly(A)聚腺苷酸化，以及從細胞核到細胞質的運輸；發現這些結合蛋白在正常情況下套用於含內含子的內源性基因的表達和加工。這項研究指出無戰組兆內含子基因可以“綁架”細胞內正常基因表達和加工的所需因子，轉為己用(NAR 2005; MCB 2005)。確認了高等動物中存在著細胞核里的基因干擾現象；發現了非編碼RNA從細胞質轉運到細胞核的新途徑；發現了nrde 通路；證明了非編碼的小RNA可以結合到正在被訂多采殼轉錄的轉錄子上；發現細胞核RNA干擾的機理可能是通過抑制RNA聚合酶II，抑制轉錄的延伸，從而造成轉錄的提前終止；發現高等動物中非編碼的小干擾RNA可以造成組蛋白的甲基化，而且這部分甲基化完全依賴於nrde基因；發展了模式生物整體上RNA-蛋白的免疫沉澱方法格您體；發展了模式生物整體水平上轉錄活性的測量方法(Science, 2008；Nature, 2010；PLoS Genetics，2011)；發現了生物葛府頸體獲得性性狀多代遺傳的分子機制，即通過小RNA的遺傳和擴增來維持表觀遺傳修飾(Nature Genetics，2012）；解析了RNA干擾過程中錯靶現象的分子凶束希鴉機制以及細胞核RNA干擾通路的生物學功能，這一通路主要調控重複序列來維持基因組穩定性(Genetics, 2014)；發現了非編碼RNA調控表觀遺傳的新機制，小RNA可以通過細胞核RNA干擾通路來誘導組蛋白3賴氨酸27的三甲基化(Curr. Biol., 2015)；發現了一類受環境刺激調控的針對rRNA的小干擾RNA(risiRNA)可以通過細胞核RNA干擾通路調控rRNA表達，並防止錯誤的rRNA積累，發現了抑制risiRNA產生的Susi通路，發現了一種新的rRNA穩態調節機制(Nat Struct Mol Biol，2017；PNAS, 2018)；發現細胞質Argonaute蛋白WAGO-4可能參與了親代到子代的siRNA的傳遞，從而介導了RNA干擾性狀的多代遺傳(Cell Reports, 2018)；發現了介導21U-RNA轉錄起始的上游序列轉錄複合物(USTC)(Genes & Development, 2019)；發展了基因編輯的新方法，通過使用CRISPR系統敲除大片段染色體以及誘導染色體易位，以及結合CRISRP和LoxP技術誘導染色體複雜區間的倒置，而後兩諒詢者為研究生物體關鍵和致死基因提供了新的手段(Sci. Rep., 2014；Genetics,2015; G3。
在包括Nature和Science在內的國際著名期刊上發表論文12篇（其中第一作者6篇），2篇論文被Faculty of 1000 Biology收錄並給予高度評價，曾獲得中國科學院院長獎及美國心臟協會博士後獎。於2011年建立中國科學技術大學分子遺傳學實驗室。
論文專著
1. Feng X, and Guang S. (2013) Small RNAs, RNAi and the Inheritance of Gene Silencing in Caenorhabditis elegans. J Genet Genomics. 2013 Apr 20;40(4):153-60.
2. Sam Guoping Gu, Julia Pak, Shouhong Guang, Jay M. Maniar, Scott Kennedy, and Andrew Fire, (2012) Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint. Nature Genetics 44:157-164.
3. Burkhart, K.B., Guang, S., Bochner, A.F., and Kennedy, S., (2011) A pre-mRNA–associating factor links endogenous siRNAs to chromatin regulation. PLoS Genetics7(8).
4. Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Burton, N., and Kennedy, S., (2010) Small regulatory RNAs inhibit RNA Polymerase II during the elongation phase of transcription. Nature, 465:1097-1101
5. Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Harding, S., Lachowiec, J., and Kennedy, S., (2008) An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science321:537-541 Research Article
6. Guang, S., Felthauser, A., and Mertz, J. (2005) Binding of hnRNP L to the pre-mRNA processing enhancer (PPE) of herpes simplex virus’ thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs. Mol. Cell. Biol.25:6303-6313.
7. Guang, S. and Mertz, J.E. (2005) PPE-like elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes. Nucleic Acid Res.33(7):2215-2226.
8. Zeng, W., Zhang, X., Tu, X., Guang, S., Xiao, Y., and Shi, Y. (2001) Expression and purification of the DNA-binding domain of the human transcription factor E2F1. Protein Expr. Purif.21(1):99-104.
9. Xia, Y.L., Wu, J.H., Guang, S., and Shi, Y. (2000) Proton NMR investigation of heme and surrounding proton in low-spin cyanide-ligated bacterial hemoglobin from vitreoscilla.Sci. China Ser (China). C43(1):57-67.
10. Xia, Y.L., Wu, J.H., Guang, S., and Shi, Y. (1998) The application of nuclear magnetic resonance in paramagnetic metallic protein - a vitreoscilla hemoglobin. Acta Biophysica Sinica (China)14(3):392-400.
11. Guang, S., Wu, J.H., Yu, T., Xia, Y.L., and Shi, Y. (1998) Solution structure of a fragment of the dimerization domain of DP-1 determined by 1H-nuclear magnetic resonance and distance geometry. Biochim. Biophys. Acta - Protein Structure and Molecular Enzymology1429(1):18-28.
12. Guang, S., Wu, J.H., Tao, L., Xia, Y.L., and Shi, Y. (1998) Solution structure of a fragment of the dimerization domain of E2F-1 determined by circular dichroism, 1H-nuclear magnetic resonance and distance geometry. Biochim. Biophys. Acta - Protein Structure and Molecular Enzymology1388(1):111-122.
在包括Nature和Science在內的國際著名期刊上發表論文12篇（其中第一作者6篇），2篇論文被Faculty of 1000 Biology收錄並給予高度評價，曾獲得中國科學院院長獎及美國心臟協會博士後獎。於2011年建立中國科學技術大學分子遺傳學實驗室。
論文專著
1. Feng X, and Guang S. (2013) Small RNAs, RNAi and the Inheritance of Gene Silencing in Caenorhabditis elegans. J Genet Genomics. 2013 Apr 20;40(4):153-60.
2. Sam Guoping Gu, Julia Pak, Shouhong Guang, Jay M. Maniar, Scott Kennedy, and Andrew Fire, (2012) Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint. Nature Genetics 44:157-164.
3. Burkhart, K.B., Guang, S., Bochner, A.F., and Kennedy, S., (2011) A pre-mRNA–associating factor links endogenous siRNAs to chromatin regulation. PLoS Genetics7(8).
4. Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Burton, N., and Kennedy, S., (2010) Small regulatory RNAs inhibit RNA Polymerase II during the elongation phase of transcription. Nature, 465:1097-1101
5. Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Harding, S., Lachowiec, J., and Kennedy, S., (2008) An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science321:537-541 Research Article
6. Guang, S., Felthauser, A., and Mertz, J. (2005) Binding of hnRNP L to the pre-mRNA processing enhancer (PPE) of herpes simplex virus’ thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs. Mol. Cell. Biol.25:6303-6313.
7. Guang, S. and Mertz, J.E. (2005) PPE-like elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes. Nucleic Acid Res.33(7):2215-2226.
8. Zeng, W., Zhang, X., Tu, X., Guang, S., Xiao, Y., and Shi, Y. (2001) Expression and purification of the DNA-binding domain of the human transcription factor E2F1. Protein Expr. Purif.21(1):99-104.
9. Xia, Y.L., Wu, J.H., Guang, S., and Shi, Y. (2000) Proton NMR investigation of heme and surrounding proton in low-spin cyanide-ligated bacterial hemoglobin from vitreoscilla.Sci. China Ser (China). C43(1):57-67.
10. Xia, Y.L., Wu, J.H., Guang, S., and Shi, Y. (1998) The application of nuclear magnetic resonance in paramagnetic metallic protein - a vitreoscilla hemoglobin. Acta Biophysica Sinica (China)14(3):392-400.
11. Guang, S., Wu, J.H., Yu, T., Xia, Y.L., and Shi, Y. (1998) Solution structure of a fragment of the dimerization domain of DP-1 determined by 1H-nuclear magnetic resonance and distance geometry. Biochim. Biophys. Acta - Protein Structure and Molecular Enzymology1429(1):18-28.
12. Guang, S., Wu, J.H., Tao, L., Xia, Y.L., and Shi, Y. (1998) Solution structure of a fragment of the dimerization domain of E2F-1 determined by circular dichroism, 1H-nuclear magnetic resonance and distance geometry. Biochim. Biophys. Acta - Protein Structure and Molecular Enzymology1388(1):111-122.
