丁侃(研究員、博士生導師)

丁侃，1996 年 7 月在中國藥科大學獲生藥學專業碩士學位， 1999 年 8 月在中國科學院上海藥物研究所獲有機化學博士學位。 1999 年 9 月至 2005 年 11 月年分別在瑞典隆德大學 (Lund University) ，美國加州大學、歐文分校 (University of California, Irvine) ，美國哈佛大學醫學院、麻省總院 (Harvard Medical School/MGH, Harvard University) 進行博士後訓練， 2005 年 12月，被中國科學院以“百人計畫”形式引進回國，始任中國科學院上海藥物研究所研究員，博士生導師。

基本介紹

• 中文名：丁侃
• 國籍：中國
• 民族：漢族
• 職業：中國科學院上海藥物研究所研究員，博士生導師
• 畢業院校：中國藥科大學獲生藥學專業

人物經歷

教育經歷

1993.09.01-1996.07.01，中國藥科大學，生藥學專業，理學碩士。師從李萍教授和中藥學家徐國鈞院士。
1996.09.01-1999.07.01，中國科學院上海藥物研究所，有機化學專業（中藥多糖化學研究方向），理學博士。師從多糖化學專家方積年研究員。

工作經歷

1986.08.01-1993.08.31，江蘇藥科學校（現為中國藥科大學高等職業技術學院），教師
1999.09.01-2001.11.01，瑞典隆德大學(Lund University)，細胞與分子生物學系，博士後，細胞與分子生物學領域（糖生物學研究方向）。師從糖生物學家Lars-Åke Fransson教授從事細胞膜蛋白聚糖結構與功能研究。
2001.12.01-2003.12.01，美國加州大學歐文分校，發育與細胞生物學系，博士後(Research Fellow, associate)，細胞與分子生物研究領域(糖生物學研究方向)。師從糖生物學家Arthur D. Lander教授從事蛋白聚糖的功能機制研究。
2004.01.01-2005.11.01，美國哈佛大學醫學院，麻省總院神經科學研究中心，博士後(Research Fellow)，細胞與分子生物學研究領域（糖生物學與腦中風研究方向）。師從腦神經學家Michael A. Moskowitz教授和Christian Waeber博士研究第二信使分子和蛋白聚糖在腦中風中的功能機制。
2006.01.01-至今，中國科學院上海藥物研究所，研究員，博士生導師，課題組長，“百人計畫”擇優獲得者，國家傑出青年基金獲得者。從事細胞膜蛋白聚糖及其相關酶和miRNA的功能機制研究。同時進行中藥多糖的作用機制，多糖靶分子發現，活性機制，構效關係及中藥多糖藥物發現的研究。

社會職務

現任美國功能性糖組學國際科學研究計畫參與研究員，國家食品藥品監督管理局藥品審評中心評審專家，中國生物化學與分子生物學會糖複合物專業委員會常委委員，國際期刊“Open Glycoscience”編委。

研究方向

現主要從事腫瘤中細胞膜蛋白聚糖結構與功能機制研究，多糖和寡糖的生物活性，構效關係，和作用機制研究，並在此基礎上從事基於糖的藥物新靶標和新藥發現研究。

主要貢獻

科研成果

建立了糖類藥物研發平台，已為國內外大學，科研院所和企業服務提供多糖，寡糖分離純化，結構鑑定，純度分析，工藝改造，質量控制，功能機制研究等多項服務。

建立了兩個新藥篩選平台：一個是靶向轉錄因子Id1的抗腫瘤藥物篩選平台，一個是抗炎藥物和免疫系統藥物篩選平台。

從300百多種中草藥中提取、純化700餘種多糖組分及化合物，鑑定了部分結構。

發現25個NF-kappaB抑制劑，70個 NF-kappaB激活劑，22個抗癌生物活性化合物。其中5個可以成為候選藥。

申請4項專利（一項國際專利），獲準一項專利，研究成果主要發表在Journal of Cell Biology，Journal of Biological Chemistry，Cancer Letters, Carbohydrate Research, Carbohydrate Polymers，Journal of Natural Products等期刊上，共33篇。

代表論著

1.Li J, Fang J, Qin Y, Liao W, Liu H, Zhou Y*, Ding K*. Glce regulates PC12 cell neuritogenesis induced by nerve growth factor through activating Smad/Id3 signaling. Biochem J. 2014, 459(2): 405-416 (*Corresponding author).

2.Xiao F, Qiu H, Cui H, Ni X, Li J, Liao W, Lu N, Ding K*. MicroRNA-885-3p inhibits the growth of HT-29 colon cancer cell xenografts by disrupting angiogenesis via targeting BMPR1A and blocking BMP/Smad/Id1 signaling. Oncogene, 2014, Jun 2;0. doi: 10.1038/onc.2014.134.

3.Liu H, Zhou L, Shi S, Wang Y, Ni X, Xiao F, Wang S, Li P* Ding K*. Oligosaccharide G19 inhibits U-87 MG human glioma cells growth in vitro and in vivo by targeting epidermal growth factor (EGF) and activating p53/p21 signaling. Glycobiology, 2014 May 5. pii: cwu038. [Epub ahead of print].

4.Cong Q, Shang M, Dong Q*, Liao W, Xiao F, Ding K*. Structure and activities of a novel heteroxylan from Cassia obtusifolia seeds and its sulfated derivative. Carbohydr Res. 2014; 393C:43-50.

5.Cong Q, Xiao F, Liao W, Dong Q*, Ding K*. Structure and biological activities of an alginate from Sargassum fusiforme, and its sulfated derivative. Int J Biol Macromol. 2014, May 28. pii: S0141-8130(14)00352-3. doi: 10.1016/j.ijbiomac.2014.05.056.

6.Wang P, Liao W, Fang J, Liu Q, Yao J, Hu M, Ding K*. A glucan isolated from flowers of Lonicera japonica Thunb. inhibits aggregation and neurotoxicity of Aβ42. Carbohydr. Polym. 2014, 110C:142-147.

7.Wang Y, Shen X, Liao W, Fang J, Chen X, Dong Q, Ding K*. A heteropolysaccharide, l-fuco-d-manno-1,6-d-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative. Carbohydr Polym. 2014, 101: 631-41.

8.Shi L*, Dong Q, Ding K*. Structure elucidation and immunomodulatory activity in vitro of a xylan from roots of Cudrania tricuspidata. 2014，Food Chem. 152：291-296.

9.Peipei Wang, Kan Ding*. Proteoglycans and Glycosaminoglycans in Misfolded Proteins Formation in Alzheimer’s Disease. Protein Pept. Lett. 2014, (Reference #: K_DING_PPL_ Manuscript_20130610, in press)

10.Wang Y, Fang J, Ni X, Li J, Liu Q, Dong Q, Duan J*, Ding K*. Inducement of Cytokine Release by GFPBW2, a Novel Polysaccharide from Fruit Bodies of Grifola frondosa, through Dectin-1 in Macrophages. J Agric Food Chem. 2013, 61(47):11400-9..

11.Xiao F, Qiu H, Zhou L, Shen X, Yang L, Ding K*. WSS25 inhibits Dicer, downregulating microRNA-210 which targets Ephrin-A3, to suppress human microvascular endothelial cell (HMEC-1) tube formation. Glycobiology. 2013, 23(5):524-35.

12.Zhang, X Liao, W Cong, Q Dong, Q* Ding, K*. Isolation and structural characterization of the polysaccharides of cortex mori radices. Acta Chimica Sinica, 2013, DOI:10.6023/A13010109

13.Feng C, Li J, Ruan J, Ding K*. MicroRNA-125a inhibits cell growth by targeting glypican-4. Glycoconj J. 2012, 29(7):503-11.

14.Shang M, Zhang X, Dong Q,* Yao J, Liu Q, Ding K* Isolation and structural characterization of the water-extractable polysaccharides from Cassia obtusifolia seeds. Carbohydr. Polym, 2012, 90(2):827-32.

15.Liu Y, Fang J, Cai H, Xiao F, Ding K*, Hu Y*. Identification and synthesis of substituted pyrrolo[2,3-d]pyrimidines as novel firefly luciferase inhibitors. Bioorg Med Chem. 2012, 20(18):5473-82.

16.Chen X, Xiao F, Wang Y, Fang J, Ding K* Structure-activity relationship study of WSS25 derivatives with anti-angiogenesis effects. Glycoconj J. 2012, 29(5-6):389-98.

17.Fang J, Wang Y, Lv X, Shen X, Ni X, Ding K*. Structure of a β-glucan from Grifola frondosa and its antitumor effect by activating Dectin-1/Syk/NF-κB signaling. Glycoconj J. 2012, 29(5-6): 365-77.

18.Fang JP, Liu Y, Li J, Liao WF, Hu YH*, Ding K*.A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-κB signaling and improves survival in mouse models of sepsis. Acta Pharmacol Sin. 2012, 33(9):1204-16.

19.Lv XF, Chen SH, Li J, Fang JP, Guo YW*, Ding K*. Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro. Acta Pharmacol Sin. 2012, 33(10):1293-300.

20.Dong Q, Wang Y, Shi L, Yao J, Li J, Ma F, Ding K *. A novel water-soluble β-D-glucan isolated from the spores of Ganoderma lucidum. Carbohydr Res. 2012, 353:100-5.

21.Cui H, Shao C, Liu Q, Yu W, Fang J, Yu W, Ali A, Ding K*. Heparanase enhances nerve growth factor induced PC12 cell neuritogenesis via the p38 MAPK pathway. Biochem J. 2011, 440(2)273-282.

22.Shen X, Fang J, Lv X, Pei Z, Wang Y, Jiang S, Ding K*. Heparin impairs angiogenesis through inhibition of MicroRNA-10b. J Biol Chem. 2011, 286(30):26616-272.

23.Chen X, Cao D, Zhou L, Jin H, Dong Q, Yao J, Ding K*. Structure of a polysaccharide from Gastrodia elata Bl., and oligosaccharides prepared thereof with anti-pancreatic cancer cell growth activities. Carbohydr Polym. 2011, 86:1300-1305.

24.Xu Y, Dong Q, Qiu H, Ma, C-W, Ding K*. A homogalacturonan from radix of Platycodon grandiflorum and the anti-angiogenesis activity of poly-/oligogalacturonic acids derived thereof. Carbohydr Res. 2011, 346(13):1930-6.

25.Qiu H, Yang B, Pei ZC, Zhang Z, Ding K*.WSS25 inhibits growth of xenografted hepatocellular cancer cells in nude mice by disrupting angiogenesis via blocking BMP/SMAD/ID1 signaling. J Biol Chem. 2010, 285(42):32638-46.

26.Xu Y, Dong Q*, Qiu H, Cong R, Ding K*. Structural characterization of an arabinogalactan from Platycodon grandiflorum roots and anti-angiogenic activity of its sulfated derivative. 2010, Biomacromolecules, 2010, 11(10):2558-66.

27.Dong Q, Liu X, Yao J, Dong X, Ma C, Xu Y, Fang J, Ding K*. Structural characterization of a pectic polysaccharide from Nerium indicum flowers. Phytochemistry, 2010, 71:1430–1437.

28.Duan J, Chen VL, Dong Q, Ding K*, Fang J. Chemical structure and immunoinhibitory activity of a pectic polysaccharide containing glucuronic acid from the leaves of Diospyros kaki. Int J Biol Macromol. 2010, 46(5):465-70.

29.Duan J, Dong Q, Ding K*, Fang J. Characterization of A pectic polysaccharide from the leaves of diospyros kaki and its modulating activity on lymphocyte proliferation. Biopolymers. 2010, 93(7),649-656

30.Tong X, Qiu H, Zhang X, Shi L, Wang G, Ji F, Ding H, Tang W*, Ding K* Zuo J*. WSS45, a sulfated α-D-glucan compound, strongly interfere with Dengue 2 virus infection in vitro, Acta Pharmacologica Sinica, 2010, 31(5):585-92

31.Wang S, Zhang Z, Lin X, Xu DS, Feng Y*, Ding K*. A Polysaccharides, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart. Glycobiology. 2010, 20 (4): 473-484.

32.Zhang Z, Wang S, Qiu H, Duan C, Ding K*, Wang Z*. Waltonitone induces human hepatocellular carcinoma cells apoptosis in vitro and in vivo. Cancer Lett, 2009, 286: 223–231.

33.Hu K, Liu Q, Wang S, Ding K*. New oligosaccharides prepared by acid hydrolysis of the polysaccharides from Nerium indicum Mill and their anti- angiogenesis activities. Carbohydr. Res., 2009, 344：198–203.