《Sphingomonas sp. BHC-A降解δ-HCH的機制研究》是洪青為項目負責人，南京農業大學為依託單位的面上項目。
基本介紹
- 中文名：Sphingomonas sp. BHC-A降解δ-HCH的機制研究
- 項目類別：面上項目
- 項目負責人 ：洪青
- 依託單位 ：南京農業大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Characterization of a Newly Isolated Highly-Effective 3, 5, 6-Trichloro-2-Pyridinol Degrading Strain Cupriavidus pauculus P2. | 期刊論文 | Li Cao|Hongming Liu|Hao Zhang|Ke Huang|Tao Gu|Haiyan Ni|Qing Hong|Shunpeng Li| |
2 | Flavobacterium yanchengense sp. nov., a bacterium isolated from soil | 期刊論文 | Gang Hu|Jun Zhang|Guiqin Yang|Yangyag Li|Yiting Guan|Jun Wang|Shunpeng Li|Qing Hong| |
3 | 六六六微生物降解途徑的研究進展 | 期刊論文 | 曹禮|張浩|黃科|谷濤|洪青|李順鵬| |
4 | Burkholderia zhejiangensis sp nov., a methyl-parathion-degrading bacterium isolated from a wastewater-treatment system | 期刊論文 | Lu, Peng|Zheng, Liu-Qiang|Sun, Jin-Jin|Liu, Hong-Ming|Li, Shun-Peng|Hong, Qing|Li, Wen-Jun| |
5 | Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by Cupriavidus sp. DT-1. | 期刊論文 | Peng Lu|Qinfen Li|Hongmin Liu|Xin Yan|Qing Hong|Shunpeng Li| |
6 | Characterization and analysis of three newly isolated hexachlorocyclohexane (HCH) -degrading strains | 期刊論文 | Li Cao|Jianhong Xu|Mingxing Li|Guang Wu|Jun Wang|Yiting Guan|Jian He|Shunpeng Li|Qing Hong| |
7 | Extension of Sphingobium sp BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering | 期刊論文 | Ge, Feng|Chen, Xu|Wang, Xin|Liao, Xuewei|Jiao, Yiying|Hong, Qing|Zhang, Longjiang|Wu, Jun| |
8 | Identification of two combined genes responsible for dechlorination of 3,5,6-trichloro-2-pyridinol (TCP) in Cupriavidus pauculus P2 | 期刊論文 | Li Cao|Jianhong Xu|Guang Wu|Mingxing Li|JIandong Jiang|Jian He|Shunpeng Li|Qing Hong| |
9 | Characterization of the propanil biodegradation pathway in Sphingomonas sp. Y57 and cloning of the propanil hydrolase gene prpH. | 期刊論文 | Ji Zhang|Ji-Quan Sun|Qiao-Yun Yuan|Chao Li|Xin Yan*|Qing Hong*|Shun-Peng Li| |
10 | Isolation of a heavy metal-resistant 4-Chloronitrobenzene degrader Cupriavidus sp D4 and cloning of its cnb genes | 期刊論文 | Wu, Zhiguo|Liu, Yalong|Zhang, Ji|Shen, Weiliang|Lu, Wenxiang|Hong, Qing|Li, Shunpeng| |
11 | Salinicola zeshunii sp. nov., a moderately halophilic bacterium isolated from soil of a chicken farm | 期刊論文 | Li Cao|Qiuxiang Yan|Haiyan Ni|Gang Hu|Qing Hong|Shunpeng Li| |
12 | Biodegradation of butachlor by Rhodococcus sp. strain B1 and purification of its hydrolase （ChlH）responsible for N-dealkylation of chloroacetamide herbicides | 期刊論文 | Hong-Ming Liu|Li Cao|Peng Lu|Haiyan Ni|Yun-Xiang Li|Xin Yan|Qing Hong|Shun-Peng Li| |
13 | Characterization of the nitrobenzene-degrading strain Pseudomonas sp. a3 and use of its immobilized cells in the treatment of mixed aromatics wastewater. | 期刊論文 | Zhiguo Wu|Yalong Liu|Hongming Liu|Yali Xia|Wenjing Shen|Qing Hong|Shunpeng Li Hangyong Yao.| |
項目摘要
六六六（HCH）是一種曾經廣泛使用的長殘留殺蟲劑。它有α、β、γ和δ四種異構體，其中γ-HCH與α-HCH的微生物降解途徑已被闡明。目前，δ-HCH的微生物降解是HCH降解領域的研究熱點。研究δ-HCH的降解途徑及相關基因對於全面了解六六六微生物降解機制具有重要意義。本課題組已在δ-HCH微生物降解方面取得了一些進展。分離到一株能高效降解六六六四種異構體的菌株Sphingomonas sp. BHC-A，研究了其降解δ-HCH的部分途徑。本項目擬進一步深入研究該菌株對δ-TDOL和δ-2,3,5-TCDL（已證明的BHC-A菌株降解δ-HCH的中間代謝產物）的降解途徑，最終推導出完整的δ-HCH降解途徑。並通過轉座子隨機插入突變和SEFA-PCR相結合的方法克隆出降解途徑中的關鍵酶基因。預期結果有助於闡明微生物降解δ-HCH的分子機理，並為微生物修復δ-HCH污染環境提供理論依據。
