《DegP分子伴侶和蛋白酶雙功能的單分子探測》是趙新生為項目負責人，北京大學為依託單位的面上項目。
基本介紹
- 中文名：DegP分子伴侶和蛋白酶雙功能的單分子探測
- 項目類別：面上項目
- 項目負責人 ：趙新生
- 依託單位 ：北京大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution | 期刊論文 | Ge, Jing|Liu, Zhaofang|Zhi, Zeyong|Zhao, Xin Sheng| |
2 | Quantitative detection of adenosine in urine using silver enhancement of aptamer-gold nanoparticle aggregation and progressive dilution | 期刊論文 | Liu, Zhao Fang|Ge, Jing|Zhao, Xin Sheng| |
3 | Amplitude of Relaxations in Fluorescence Correlation Spectroscopy for Fluorophores That Diffuse Together | 期刊論文 | Yandong Yin|Rongfeng Yuan|XIN SHENG ZHAO| |
4 | Cocaine Detection in Blood Serum Using Aptamer Biosensor on Gold Nanoparticles and Progressive Dilution | 期刊論文 | Jing Ge|Zhao Fang Liu|XIN SHENG ZHAO| |
5 | Direct Observation of the Uptake of Outer Membrane Proteins by the Periplasmic Chaperone Skp | 期刊論文 | Zhi-Xin Lyu|Qiang Shao|Yi Qin Gao|XIN SHENG ZHAO| |
6 | Ultrafast Photoinduced Electron Transfer between Tetramethylrhodamine and Guanosine in Aqueous Solution | 期刊論文 | Li, Xun|Zhu, Ruixue|Yu, Anchi|Zhao, Xin Sheng| |
7 | A Microfluidic Mixer for Single-Molecule Kinetics Experiments | 期刊論文 | Zhi Ze-Yong|Liu Peng-Cheng|Huang Yan-Yi|Zhao Xin-Sheng| |
8 | Kinetic and thermodynamic characterization of the reaction pathway of box H/ACA RNA-guided pseudouridine formation | 期刊論文 | Xinxing Yang|Jingqi Duan|Shuang Li|Peng Wang|Shoucai Ma|Keqiong Ye|XIN SHENG ZHAO| |
9 | Maximum Entropy Method for Analyses of Fluorescence Correlation Spectra of Oligonucleotide Intra-Chain Collision | 期刊論文 | Yin Yan-Dong|Zhou Xiao-Xue|Zhao Xin-Sheng| |
10 | Interaction between bacterial outer membrane proteins and periplasmic quality control factors: a kinetic partitioning mechanism | 期刊論文 | Wu, Si|Ge, Xi|Lv, Zhixin|Zhi, Zeyong|Chang, Zengyi|Zhao, Xin Sheng| |
11 | Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy | 期刊論文 | Yandong Yin|Peng Wang|Xin Xing Yang|Xun Li|Chuan He|XIN SHENG ZHAO| |
12 | Kinetics and Dynamics of DNA Hybridization | 期刊論文 | Yin, Yandong|Zhao, Xin Sheng| |
13 | Photophysical Properties of Atto655 Dye in the Presence of Guanosine and Tryptophan in Aqueous Solution | 期刊論文 | Zhu, Ruixue|Li, Xun|Zhao, Xin Sheng|Yu, Anchi| |
14 | Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5 | 期刊論文 | Li, Xun|Yin, Yandong|Yang, Xinxing|Zhi, Zeyong|Zhao, Xin Sheng| |
15 | Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides | 期刊論文 | Qu, Peng|Yang, Xinxing|Li, Xun|Zhou, Xiaoxue|Zhao, Xin Sheng| |
16 | Domain-Specific Folding Kinetics of Staphylococcal Nuclease Observed through Single-Molecule FRET in a Microfluidic Mixer | 期刊論文 | Zhi, Zeyong|Liu, Pengcheng|Wang, Peng|Huang, Yanyi|Zhao, Xin Sheng| |
項目摘要
廣泛存在於生物體內的HtrA蛋白質家族是一類重要的熱休克蛋白，同時具有分子伴侶和蛋白酶的雙重功能，在生物體對蛋白質進行質量控制中起關鍵作用。人類的HtrA同源蛋白質與各種疾病如癌症、帕金森症、老年痴呆症都有關係。HtrA在大腸桿菌中被稱作DegP，對於細菌外膜蛋白的生物合成和質量控制至關重要。我們擬以DegP為對象，採用單分子探測技術研究HtrA家族的分子伴侶和蛋白酶雙重功能的分子機制。將單分子探測這一物理化學新方法引入相關研究，可以揭示出新的現象和規律，增進人們對生物體實現蛋白質的正確摺疊、抵禦外部脅迫環境、清除錯誤摺疊的蛋白質的機理的了解，為人類認識相關疾病的發病機理和提出治療對策提供科學依據。這是一個有相當難度的課題，但我們經過多年的探索與準備，已經具備了實現預定目標的研究基礎，可以圓滿完成任務。
