Comprehensive experiment of life science

內容簡介

生物化學和細胞生物學是生命科學的重要基礎學科其基本實驗技術和方法更是現代生命科學研究的基石。本教材的目的是使醫學院校本科學生初步掌握生物化學和細胞生物學基本實驗原理、方法和技術，為將來的科學研究奠定基礎。

Preface

Part 1 Biochemistry Experiment

Experiment 1 Use of the Spectrophotometer & Determination of Protein Concentration

Experiment 2 Chromatography

Experiment 2 (1) Using Gel Filtration Column Chromatography to Separate Hemoglobin and Nucleoprotamine

Experiment 2 (2) Separating Amino Acids Mixture by Lon-Exchange Chromatography

Experiment 3 Determination of Km of Alkaline Phnsphatase

Experiment 4 Centrifugation Technique--Adaptive Immune Response: Isolation and Identification of Lymphocyte

Experiment 4 (1) The Separation of Monocytes by Density Gradient Centrifugation

Experiment 4 (2) Lymphocyte Identification--Counting the Number of B Lymphocyte

Experiment 5 Polyacrylamide Gel Electrophoresis of Proteins

Experiment 6 Effect of Hormone on Blood Sugar and Lipoprotein of Normal and Diabetic rat

Experiment 7 Polymorphism Analysis of ACE Gene by Extracting Genome DNA of Buccal Epithelial Cells

Part 2 Cell Biology Experiment

Experiment 8 Optical Microscope and Cell Cycle