魏澤文

魏澤文

魏澤文，男，北京理工大學生物醫學工程系副教授（研究員）/ 博士生導師，微電子機械系統方向。主要研究微電子機械系統在生物醫學工程領域的套用，側重將前沿MEMS技術套用於生物醫療領域，包括利用基於矽微加工技術的微納器件和基於軟光刻的微流控器件對蛋白、核酸、細胞及組織進行精細分析及操控。發表SCI及本領域頂級會議論文40餘篇，多為JCR一區頂刊，H因子14，申請專利十餘項，出版書籍一章節。

基本介紹

  • 中文名：魏澤文
  • 國籍：中國
  • 民族：漢
  • 畢業院校：北京大學
  • 專業方向：生物醫學工程
  • 職務：北京理工大學博士生導師
人物經歷,工作經歷,教育經歷,科學研究,研究課題,研究方向,科研項目,社會兼職,科研成果,SCI論文,頂級論文,出版書籍,專利發明,工作單位,

人物經歷

工作經歷

2019.4至今北京理工大學生命學院研究員，博士生導師；
2011.7－2019.03 國家納米科學中心副研究員；
2016.1－2016.12哈佛醫學院國家公派訪問學者；

教育經歷

2007.9－2011.7北京大學信息科學與技術學院獲理學博士學位；
2004.9－2007.7東南大學電子科學與工程學院獲工學碩士學位；
2000.9－2007.7東南大學電子科學與工程學院獲工學學士學位。

科學研究

研究課題

微電子機械系統(Micro-Electro-Mechanical System)及其在生物醫學工程領域的套用(Bio-MEMS)。微電子機械系統（MEMS）是在微電子技術的基礎上發展起來的，融合了矽微加工，並套用現代信息技術構成的微型系統。它包括感知和控制外界信息(力、熱、光、磁、化)的感測器和執行器，以及進行信號處理的電路。從國防到工業控制，從醫療設備到消費電子，MEMS技術都有廣泛的套用。本人的研究領域是將前沿MEMS技術套用於生物醫療領域，包括利用基於矽微加工技術的微納器件和基於軟光刻的微流控器件對蛋白、核酸、細胞及組織進行精細分析及操控。

研究方向

（1）液體活檢、稀有細胞檢測及單細胞操控、分析和測序
探索多種前沿的微結構、微器件和生物分析方法，實現對細胞（及其行為）、蛋白與核酸的精確操控、篩選和分析，用於多種疾病的體外診斷。例如：
首次將循環腫瘤細胞檢測的所有步驟（包括血液處理、細胞分離、鑑定、計數及單細胞分析和基因擴增）都集成在單塊微流控晶片上，結合基於深度學習的圖像分析算法，實現了在3.5小時內處理2毫升全血並無需人工干預，在近200例臨床試驗中獲得了迄今為止最優的性能。該系列技術有望成為癌症液體活檢及癌症單細胞測序的有效工具。
（2）基於單細胞操控的單克隆抗體系列新技術
基於多種新穎的微結構及單細胞測序技術，在單克隆抗體生成、篩選、測序領域開發了一系列新方法和新技術。
（3）基於細胞電穿孔(Electroporation)的核酸輸運
探索了多種微電極結構，全面提升了向體外細胞或活體組織輸運核酸的效率和通量。用於體外細胞的系列成果已轉讓形成產品，性能顯著優於國外同類產品。用於活體組織的系列成果已進入DNA疫苗等治療領域，例如向恆河猴遞送針對HIV的DNA疫苗，已取得優秀的免疫回響（抗體滴度比現有金標準痘病毒遞送高60%）和保護效果。該系列技術不僅能夠顯著提高電穿孔實驗技術的效率和通量，還有望使電穿孔技術成為提升核酸藥物療效的有力工具。

科研項目

主持或參與多項國家自然科學基金、863計畫、中科院科技發展項目及北京市科委重點項目

社會兼職

Lab on a Chip, Theranostics, Nanoscale, Analyst等多個知名學術期刊審稿人
中國醫療保健國際交流促進會健康大數據和數位化醫療分會委員
IEEE 會員

科研成果

SCI論文

  1. Ren Li*, Mingxing Zhou, Chunyan Yue, Weikai Zhang, Yan Ma, Hailing Peng, Zhiyuan Hu#, Zewen Wei#. “Multiple Single Cell Screening and DNA MDA Amplification Chip for Oncogenic Mutation Profiling”, Lab on a Chip, Full paper, 2018, 18(5), 723-734. (IF 6.1, JCR一區) 封面文章
  2. Ren Li*, Yan Ma*, Mingzhi Zhao, Zhiyuan Hu#, Zewen Wei#. “Device to Study the Cell Invasion Behavior and Phenotypic Profile at Single Cell Level”, Analytical Chemistry, Article, 2018, 90(3), 1691-1700. (IF 6.0, JCR一區)
  3. Deyao Zhao*, Mengxi Wu, Dong Huang, Zicai Liang#, Zewen Wei#, Zhihong Li#. “Parametric Optimization of Electric Field Strength for Cancer Electrochemotherapy on A Chip-Based Model”, Theranostics. Article, 2018, 8(2), 358-368. (IF 8.5, JCR一區)
  4. Ren Li*, Mingxing Zhou*, Jine Li, Zihua Wang, Weikai Zhang, Chunyan Yue, Yan Ma, Hailing Peng, Zewen Wei#, Zhiyuan Hu#, “Identifying EGFR-Expressing Cells and Detecting EGFR Multi-Mutations at Single-Cell Level by Microfluidic Chip”, Nano-Micro Letters, Article, 2018, 10(16), s40820-017-0168-y. (IF 9.0, JCR一區)
  5. Mingzhi Zhao*, Deyao Zhao*, Yan Ma, Zhiyuan Hu#, Zewen Wei#. “Quantitative Proteomic Analysis of Cell Responses to Electroporation, a Classical Gene Delivery Approach”, Proteomics, Full paper, 2018, 16, e1800127. (IF 4.0, JCR二區)
  6. Weikai Zhang*, Zhitao Li*, Zhihua Wang, Chunyan Yue, Hui Zheng, Ren Li, Mingxing Zhou, Zhiyuan Hu#, Zewen Wei#, Qin Li#. “Generation of a Monoclonal Antibody Recognizing the Heavily Glycosylated CD45 Protein and Its Application on Identifying Circulating Tumor Cells”, PLoS One , Article, 2018, 13(2), e0192506. (IF 2.7, JCR三區)
  7. Dong Huang*, Deyao Zhao*, Xiaoxia Wang, Chunhui Li, Tongren Yang, Lili Du, Zewen Wei, Qiang Cheng, Huiqing Cao, Zicai Liang, Yuanyu Huang#, Zhihong Li#,“Efficient Delivery of Nucleic Acid Molecules into Skin by Combined Use of Microneedle Roller and Flexible Interdigitated Electroporation Array”, Theranostics. Article, 2018, 8(9), 2361-2376. (IF 8.5, JCR一區)
  8. Xiaoran Liu*, Ran Ran, Bin Shao, Hope S. Rugo, Yanlian Yang, Zhiyuan Hu, Zewen Wei, Fengling Wan, Weiyao Kong, Guohong Song, Hanfang Jiang, Xu Liang, Ruyan Zhang, Ying Yan, Guobing Xu, Huiping Li#, “Combined Peripheral Natural Killer Cell and Circulating Tumor Cell Enumeration Enhance Prognostic Efficiency in Patients with Metastatic Triple-Negative Breast Cancer”, Chinese Journal of Cancer Research. Full paper, 2018, 30(3), 315–326 (IF 1.9, JCR三區)
  9. Mingxing Zhou*, Hui Zheng*, Zihua Wang, Ren Li, Xiaoran Liu, Weikai Zhang, Huiping Li#, Zewen Wei#, Zhiyuan Hu#. “Precisely Enumerating Circulating Tumor Cells Utilizing a Multi-Functional Microfluidic Chip and Unique Image Interpretation Algorithm”, Theranostics. Article, 2017, 7(19), 4710-4721. (IF 8.5, JCR一區)
  10. Deyao Zhao*, Dong Huang*, Yan Li, Mengxi Wu, Wenfeng Zhong, Qiang Cheng, Xiaoxia Wang, Yidi Wu, Xiao Zhou, Zewen Wei#, Zhihong Li#, Zicai Liang#,“A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo”, Scientific Reports, Full paper, 2016, 6,18469. (IF 4.1, JCR三區)
  11. Min Ye*, Zewen Wei*, Fei Hu, Jianxin Wang, Guanglu Ge, Zhiyuan Hu, Mingwang Shao, Shuit-Tong Lee and Jian Liu#. “Fast Assembling Microarrays of Superparamagnetic Fe3O4@Au Nanoparticle Clusters as Reproducible Substrates for Surface-Enhanced Raman Scattering”, Nanoscale, Full paper, 2015, 7, 13427-13437. (IF 7.2, JCR一區) 封面文章
  12. Zewen Wei*, Yuanyu Huang*, Deyao Zhao, Zhihong Li#, Zicai Liang#,“A Pliable Electroporation Patch (Ep-Patch) for Efficient Delivery of Nucleic Acid Molecules into Animal Tissues with Irregular Surface Shapes”, Scientific Reports, Full paper, 2015, 5,7618. (IF 4.1, JCR三區)
  13. Zihua Wang*, Weizhi Wang*, Xiangli Bu, Zewen Wei, Lingling Geng, Yue Wu, Chengyan Dong, Liqiang Li, Di Zhang, Shu Yang, Fang Wang, Christopher Lausted, Leroy Hood, Zhiyuan Hu#, “Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor”, Analytical Chemistry, Article, 2015, 87(16), 8367-8372. (IF 6.0, JCR一區)
  14. Yang Li*, Mengxi Wu*, Deyao Zhao, Zewen Wei, Wenfeng Zhong, Xiaoxia Wang, Zicai Liang#, Zhihong Li#,“Electroporation on Microchips: the Harmful Effects of PH Changes and Scaling Down”, Scientific Reports, Full paper, 2015, 5,17817. (IF 4.1, JCR三區)
  15. Weizhi Wang*, Di Zhang*, Zewen Wei*, Zihua Wang, Xiangli Bu, Shu Yang, Qiaojun Fang#, Zhiyuan Hu#, “Label-Free Detection Microarray for Novel Peptide Ligands Screening base on MS-SPRi Combination”, Talanta, Research Paper, 2015, 134, 705-711. (IF 4.2, JCR二區)
  16. Zewen Wei*, Shuquan Zheng*, Renxing Wang, Xiangli Bu, Huailei Ma, Yidi Wu, Ling Zhu, Zhiyuan Hu#, Zicai Liang# and Zhihong Li#, “A Flexible Microneedle Array as Low-Voltage Electroporation Electrodes for In Vivo DNA and SiRNA Delivery”, Lab on a Chip, Full paper, 2014, 14, 4093-4102. (IF 6.1, JCR一區)
  17. Zewen Wei*, Xueming Li, Deyao Zhao, Hao Yan, Zhiyuan Hu#, Zicai Liang# and Zhihong Li#, “Flow-Through Cell Electroporation Microchip Integrating Dielectrophoretic Viable Cell Sorting”, Analytical Chemistry, Article, 2014, 86, 10215-10222. (IF 6.0, JCR一區)
  18. Weizhi Wang*, Zewen Wei*, Di Zhang, Huailei Ma, Zihua Wang, Xiangli Bu, Menglin Li, Lingling Geng, Christopher Lausted, Leroy Hood, Qiaojun Fang, Hao Wang, Zhihua Wang, Zhiyuan Hu#, “Rapid Screening of Peptide Probes Through In Situ Single Bead Sequencing Microarray”, Analytical Chemistry, Article, 2014, 86, 11854-11859. (IF 6.0, JCR一區)
  19. Weizhi Wang*, Zewen Wei*, Zihua Wang, Huailei Ma, Xiangli Bu, Zhiyuan Hu#, “A Continuous Flow Microfluidic-Ms System for Efficient OBOC Screening”, RSC Advances, Communication, 2014, 4, 61767-61770. (IF 2.9, JCR三區)
  20. Weizhi Wang*, Menglin Li*, Zewen Wei, Zihua Wang, Xiangli Bu, Wenjia Lai, Shu Yang, He Gong, Hui Zheng, Yuqiao Wang, Ying Liu, Qin Li#, Qiaojun Fang#, Zhiyuan Hu#, “Bimodal Imprint Chips for Peptide Screening: Integration of High-Throughput Sequencing by MS and Affinity Analyses by Surface Plasmon Resonance Imaging”, Analytical Chemistry, Letter, 2014, 86, 3703-3707. (IF 6.0, JCR一區)
  21. Mengxi Wu*, Deyao Zhao*, Zewen Wei, Wenfeng Zhong, Hao Yan, Xiaoxia Wang, Zicai Liang# and Zhihong Li#, “Method for electric parametric characterization and optimization of electroporation on a chip”, Analytical Chemistry, Article, 2014, 85, 4483-4491. (IF 6.0, JCR一區)
  22. Zewen Wei*, Deyao Zhao*, Xueming Li, Mengxi Wu, Wei Wang, Huang Huang, Xiaoxia Wang, Quan Du, Zicai Liang# and Zhihong Li#, “A Laminar Flow Electroporation System for Efficient DNA And SiRNA Delivery”, Analytical Chemistry, Article, 2011, 83, 5881-5887. (IF 6.0, JCR一區)
  23. Huang Huang*, Zewen Wei*, Yuanyu Huang, Deyao Zhao, Lianhong Zhen, Tianjing Cai, Mengxi Wu, Wei Wang, Quan Du#, Zhihong Li#, Zicai Liang#, “An Efficient and High-Throughput Electroporation Microchip Applicable for SiRNA Delivery”. Lab on a Chip, Full paper, 2011, 11, 163-172. (IF 6.1, JCR一區)

頂級論文

  1. Ren Li*, Fei Jia*, Weikai Zhang, Zhiguo Fang, Zhiyuan Hu, Zewen Wei#, “A Microfluidic Chip for CTC Whole Genome Sequencing”IEEE MEMS 2019, Accepted.
  2. Weikai Zhang*, Ren Li, Zhiguo Fang, Zhiyuan Hu, Qin Li#, Zewen Wei#, “A Microfluidic Chip for Screening High-Producing Hybridomas at Single Cell Level” IEEE MEMS 2019, Accepted.
  3. Dong Huang*, Deyao Zhao*, Jinhui Li, Lili Du, Zewen Wei, Zicai Liang#, Zhihong Li#, “A Minimally Invasive In Vivo Electroporation Method Utilizing Flexile Electrode and Microneedle Roller”, TRANSDUCERS 2017, 1684-1687.
  4. Ren Li*, Mingxing Zhou*, Jine Li, Weikai Zhang, Zihua Wang, Zewen Wei#, Zhiyuan Hu#, “Micro-Well Array Based Genetic Determination of EGFR Mutation At Single-Cell Level”, MicroTAS 2016, 206-207. 該次會議中國大陸唯一的口頭報告
  5. Ren Li*, Mingxing Zhou, Chunyan Yue, Weikai Zhang, Zewen Wei#, Zhiyuan Hu#,“Identifying EGFR-Expressed Cells and Determining the Abundances of Different EGFR Mutation Types at Single-Cell Level”, MicroTAS 2016, 369-370.
  6. Mingxing Zhou, Hui Zheng, Ren Li, Weikai Zhang, Zihua Wang, Zewen Wei#, Zhiyuan Hu#, “Precisely Enumerating CTCs from Clinical Blood Samples Utilizing Integrated Microfluidic Chip and Automatic Image Processing”, MicroTAS 2016, 299-300
  7. Zewen Wei*, Renxing Wang*, Shuquan Zheng, Zicai Liang#, Zhihong Li#, “An Electroporation Chip Based on Flexible Microneedle Array for In Vivo Nucleic Acid Delivery”, IEEE MEMS 2014, 878-820.
  8. Hui Li, Wei Wang, Huaiqiang Yu, Renxin Wang, Zewen Wei, Zhihong Li, ”Fabrication of silica opal array with high mechanical strength”, IEEE NEMS 2013, DOI: 10.1109/NEMS.2013.6559819
  9. Renxin Wang*, Zewen Wei, Wei Wang, Zhihong Li#, “Flexible Microneedle Electrode Array Based on Parylene Substrate”, MicroTAS 2012, 1249-1251.
  10. Zhihong Li*#, Zewen Wei, Xueming Li, Quan Du, Zicai Liang,“Efficient and High-Throughput Electroporation Chips”, TRANSDUCERS 2011, 1825-1828.
  11. Xueming Li*, Zewen Wei*, Deyao Zhao, Hao Yan, Zicai Liang#, Zhihong Li#, “A Flow-Through Electroporation Chip Integrated with Viable Cell Sorting Based on Dielectrophoresis”, IEEE MEMS 2012, 993-996.
  12. Mengxi Wu, Zewen Wei, Deyao Zhao, Zhihong Li, “A Portable and High Efficiency System for Cell Electroporation under Low Voltage”, IEEE NEMS 2011, DOI: 10.1109/NEMS.2011.6017467
  13. Zewen Wei*, Yuanyu Huang*, Deyao Zhao, Zicai Liang#, Zhihong Li#, “A Parylene-Based Flexible Electroporation Chip Applicable for In Vivo Gene and SiRNA Delivery”, TRANSDUCERS 2011, 1942-1945.
  14. Zewen Wei*, Huang Huang*, Mengxi Wu, Zicai Liang, Wei Wang, Zhihong Li#,“A High Performance Continuous Electroporation Chip”, MicroTAS 2010, 1622-1624.
  15. Zewen Wei*, Huang Huang*, Zicai Liang#, Zhihong Li#, “A High Performance Electroporation Chip Integrating Multi-Well Plate and Annular Interdigital Microelectrodes”, IEEE MEMS 2010, 951-954
  16. Xu Ji*, Lin Yang*, Zewen Wei, Lu Wang, Jianzhong Xi, Zhihong Li#, “A Monolithically Integrated Platform for High Throughput Cell Characterization and Drug Screening”, TRANSDUCERS 2009, 1786-1789.

出版書籍

  1. Zewen Wei, Zhihong Li, “Methods in Molecular Biology: Electroporation Protocols”, Chapter 8, 2014, New York, Humana Press, Springer.

專利發明

  1. 一種電穿孔晶片及基於電穿孔晶片的多孔板裝置 ZL200910237334.X
  2. 一種單細胞分析用微流控晶片和系統及單細胞分析方法 ZL201410141283.1
  3. 一種流式電穿孔裝置及系統 ZL201010242144.X
  4. 一種用於向活體組織中的細胞內部輸運核酸的柔性電極晶片 ZL201110150176.1
  5. 一種微腔陣列質譜靶板及其製作方法和套用 ZL201410100307.9
  6. 一種流式電穿孔裝置及系統 ZL200910237335.4
  7. 基於微機械技術的電容非熱式流速感測器 ZL200610040600.6
  8. 基於微機械系統的非熱式流速流向感測器 ZL200610040599.7
  9. 用於表達EGFR的單細胞分選和多基因位點檢測的微流控晶片 201610576642.5
  10. 用於捕獲和鑑定循環腫瘤細胞的微流控晶片 201611053450.2
  11. 用於單細胞分選和單細胞全基因組擴增的微流控晶片 201610576644.4
  12. 用於研究腫瘤單細胞侵襲和上皮間質轉化的微流控晶片 201710597990.5

工作單位

地址：北京海淀區中關村南大街5號北京理工大學生命學院
郵編：100081

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