顧敬敏

研究方向

主要從事重要耐藥性病原菌治療性噬菌體及其裂解酶和解聚酶等抗菌蛋白的生物學特性和分子作用機制等方面的研究。研究工作主要諒殼妹包括：對MRSA、VREF、CRKP、MDR-Ab等超級耐藥細菌的噬菌體進行了分離、生物學特性和治療性套用等方面的研究；並對噬菌體的抗菌相關蛋白的作用機制、抗菌和清除生物被膜的活性、及體內感染模型的治療效果等方面進行了深入研究；首次揭示了MRSA噬菌體裂解酶LysGH15的蛋白質三維結構及其發揮廣譜抗菌活性的分子作用機制。

科研項目

1.國家自然科學基金面上項目，3187130554，綠色氣球菌噬菌體新型裂解酶AVPL的生物學特性及其跨菌種抗葡萄球菌的分子機制，2019/01-2022/12，69萬元，在研，主持

2.國家重點研發計畫“畜禽少盼霉頌重大疫病防控與高效安全養殖綜合技術研發” 重點專項“動物疫病生物防宙連治性製劑研製與產業化”子課題“噬菌體裂解酶生物治療製劑研製”，2017/07-2020/12，45萬元，在研，主持

3.國家自然科學基金青年項目，31502103，金黃色葡萄球菌噬菌體穿孔素及其與裂解酶CHAP片段重端淋灑組蛋白的構建犁灶海與束付罪作用機制，2016/01-2018/12，24萬元，在研，主持

4. 國家自然科學基金面上項目，31572553，金黃色葡萄球菌噬菌體裂解酶LysGH15的抗原性及其代謝動力學，2016/01-2019/12，69.6萬元，在研，子課題主持

5.國家自然科學基金重點項目，31130072，一種新型噬菌體及其裂解騙拒連蒸酶的分子生物學特徵及作用靶位的生物結構，2012/01-2016/12，277萬元，結題，第一參加人

主要論文

[1] Cai R, Wu M, Zhang H, Zhang Y, Cheng M, Guo Z, Ji Y, Xi H, Wang X, Xue Y, Sun C, Feng X, Lei L, Tong Y, Liu X, Han W*,Gu J*. A smooth-type, phage-resistant Klebsiella pneumoniae mutant strain reveals OmpC is indispensable for GH-K3 infection.Appl Environ Microbiol. 2018 Aug 31.

[2] Zhang Y, Cheng M, Zhang H, Dai J, Guo Z, Li X, Ji Y, Cai R, Xi H, Wang X, Xue Y, Sun C, Feng X, Lei L, Han W*,Gu J*. Antibacterial effects of phage lysin LysGH15 on planktonic cells and biofilms of diverse staphylococci.Appl Environ Microbiol. 2018 May 18.

[3] Cheng M, Zhang L, Zhang H, Li X, Wang Y, Xia F, Wang B, Cai R, Guo Z, Zhang Y, Ji Y, Sun C, Feng X, Lei L, Yang Y, Han W,Gu J*. An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin Wounds.Viruses. 2018 May 6;10(5).

[4]Gu J, Xi H, Cheng M, Han W*. Phage-derived lysins as therapeutic agents against multidrug-resistant Enterococcus faecalis.Future Microbiol. 2018 Mar;13:275-278.

[5] Cheng M, Zhang Y, Li X, Liang J, Hu L, Gong P, Zhang L, Cai R, Zhang H, Ge J, Ji Y, Guo Z, Feng X, Sun C, Yang Y, Lei L, Han W*,Gu J*. Endolysin LysEF-P10 shows potential as an alternative treatment strategy for multidrug-resistant Enterococcus faecalis infections.Sci Rep. 2017 Aug 31;7(1):10164.

[6] Guo Z, Huang J, Yan G, Lei L, Wang S, Yu L, Zhou L, Gao A, Feng X, Han W,Gu J*, Yang J*. Identification and Characterization of Dpo42, a Novel Depolymerase Derived from the Escherichia coli Phage vB_EcoM_ECOO78.Front Microbiol. 2017 Aug 2;8:1460.

[7] Cheng M, Liang J, Zhang Y, Hu L, Gong P, Cai R, Zhang L, Zhang H, Ge J, Ji Y, Guo Z, Feng X, Sun C, Yang Y, Lei L, Han W*,Gu J*. The Bacteriophage EF-P29 Efficiently Protects against Lethal Vancomycin-Resistant Enterococcus faecalis and Alleviates Gut Microbiota Imbalance in a Murine Bacteremia Model.Front Microbiol. 2017 May 9;8:837.

[8] Zhang L, Li D, Li X, Hu L, Cheng M, Xia F, Gong P, Wang B, Ge J, Zhang H, Cai R, Wang Y, Sun C, Feng X, Lei L, Han W*,Gu J*. LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation.Sci Rep. 2016 Jul 7;6:29344.

[9]Gu J, Li X, Yang M, Du C, Cui Z, Gong P, Xia F, Song J, Zhang L, Li J, Yu C, Sun C, Feng X, Lei L, Han W*. Therapeutic effect of Pseudomonas aeruginosa phage YH30 on mink hemorrhagic pneumonia.Vet Microbiol. 2016 Jul 15;190:5-11.

[10] Gong P, Cheng M, Li X, Jiang H, Yu C, Kahaer N, Li J, Zhang L, Xia F, Hu L, Sun C, Feng X, Lei L, Han W,Gu J*. Characterization of Enterococcus faecium bacteriophage IME-EFm5 and its endolysin LysEFm5.Virology. 2016 May;492:11-20.

[11] Song J, Xia F, Jiang H, Li X, Hu L, Gong P, Lei L, Feng X, Sun C,Gu J*, Han W*. Identification and characterization of HolGH15: the holin of Staphylococcus aureus bacteriophage GH15.J Gen Virol. 2016 May;97(5):1272-81.

[12]Xia F, Li X, Wang B, Gong P, Xiao F, Yang M, Zhang L, Song J, Hu L, Cheng M, Sun C, Feng X, Lei L, Ouyang S, Liu ZJ, Li X,Gu J*, Han W*. Combination Therapy of LysGH15 and Apigenin as a New Strategy for Treating Pneumonia Caused by Staphylococcus aureus.Appl Environ Microbiol. 2015 Oct 16;82(1):87-94.

[13] Yang M, Du C, Gong P, Xia F, Sun C, Feng X, Lei L, Song J, Zhang L, Wang B, Xiao F, Yan X, Cui Z, Li X,Gu J*, Han W*. Therapeutic effect of the YH6 phage in a murine hemorrhagic pneumonia model.Res Microbiol. 2015 Oct;166(8):633-43.

[14]Gu J, Feng Y, Feng X, Sun C, Lei L, Ding W, Niu F, Jiao L, Yang M, Li Y, Liu X, Song J, Cui Z, Han D, Du C, Yang Y, Ouyang S, Liu ZJ, Han W. Structural and biochemical characterization reveals LysGH15 as an unprecedented "EF-hand-like" calcium-binding phage lysin.PLoS Pathog. 2014 May 15;10(5):e1004109.

[15]Gu J, Liu X, Yang M, Li Y, Sun C, Lu R, Song J, Zhang Q, Lei L, Feng X, Du C, Yu H, Yang Y, Han W. Genomic characterization of lytic Staphylococcus aureus phage GH15: providing new clues to intron shift in phages.J Gen Virol. 2013 Apr;94(Pt 4):906-15.

[16]Gu J, Liu X, Lu R, Li Y, Song J, Lei L, Sun C, Feng X, Du C, Yu H, Yang Y, Han W. Complete genome sequence of Staphylococcus aureus bacteriophage GH15.J Virol. 2012 Aug;86(16):8914-5.

[17]Gu J, Liu X, Li Y, Han W, Lei L, Yang Y, Zhao H, Gao Y, Song J, Lu R, Sun C, Feng X. A method for generation phage cocktail with great therapeutic potential.PLoS One. 2012;7(3):e31698.

[18]Gu J, Lu R, Liu X, Han W, Lei L, Gao Y, Zhao H, Li Y, Diao Y. LysGH15B, the SH3b domain of staphylococcal phage endolysin LysGH15, retains high affinity to staphylococci.Curr Microbiol. 2011 Dec;63(6):538-42.

[19]Gu J, Xu W, Lei L, Huang J, Feng X, Sun C, Du C, Zuo J, Li Y, Du T, Li L, Han W. LysGH15, a novel bacteriophage lysin, protects a murine bacteremia modelefficiently against lethal methicillin-resistant Staphylococcus aureus infection.J Clin Microbiol. 2011 Jan;49(1):111-7.（F1000論文）

近幾年以第一或通訊作者在PloS Pathogens、Applied and Environmental Microbiology、Journal of Virology和Journal of Clinical Microbiology等國際知名雜誌發表SCI論文。

顧敬敏

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科研項目

主要論文

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