《電場調製下的DNA複合物結構性能研究》是梁德海為項目負責人，北京大學為依託單位的面上項目。
基本介紹
- 中文名：電場調製下的DNA複合物結構性能研究
- 項目類別 ：面上項目
- 項目負責人：梁德海
- 依託單位 ：北京大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | 基於多肽複合物的基因載體研究 | 會議論文 | - |
2 | DNA/RNA與聚陽離子的複合動力學研究 | 期刊論文 | 周繼寒|牛林|梁德海| |
3 | Relationship between peptide structure and antimicrobial activity as studied by de novo designed peptides | 期刊論文 | Yuqiong Xia|Dong Li|Quan Du|Dehai Liang| |
4 | Complete dissociation and reassembly behavior as studied by using poly(ethylene glycol)-block-poly(glutamate sodium) and kanamycin A | 期刊論文 | Pan, Wei|Zhou, Jihan|Li, Zhibo|Liang, Dehai| |
5 | Capsules with a hierarchical shell structure assembled by aminoglycosides and DNA via the kinetic path | 期刊論文 | April A. Brisky|Nan Wang|Lin Zhu|Xiangyun Qiu| |
6 | Peptides containing blocks of different charge densities facilitate cell uptake of oligonucleotides | 期刊論文 | Su, Cuicui|Wen, Hao|Du, Quan|Liang, Dehai| |
7 | Complexation behavior of oppositely charged polyelectrolytes: Effect of charge distribution | 期刊論文 | Su, Cuicui|Niu, Lin|Liang, Dehai|Li, Baohui| |
8 | Phase separation of siRNA-polycation complex and its effect on transfection efficiency | 期刊論文 | Tao Shi|Yuqiong Xia|Ying Luo|Dehai Liang| |
9 | Structure and stability of the complex formed by oligonucleotides | 期刊論文 | Jingjing Yan|Jie Liu|Ying Luo|Dehai Liang| |
10 | Long-term kinetics of DNA interacting with polycations | 期刊論文 | Zhou, Jihan|Lv, Hua|Cheng, Jianjun|Liang, Dehai| |
11 | Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering | 期刊論文 | Burger, Christian|Rong LiXi|Hsiao, Benjamin|Liang DeHai| |
12 | Mechanism of DNA assembly as revealed by energy barriers | 期刊論文 | Zhou, Jihan|Mao, Chengde|Liang, Haojun|Liang, Dehai| |
13 | Complexation of DNA with poly-(L-lysine) and its copolymers in dimethylformamide | 期刊論文 | Jianbo Sun|Ning Xu|Zi-Chen Li|Dehai Liang| |
14 | The growth of filaments under macromolecular confinement using scaling theory | 期刊論文 | Lu, Xi|Li, Desheng|Zhao, Jiang|Liang, Dehai| |
15 | Axial Growth and Fusion of Liposome Regulated by Macromolecular Crowding and Confinement | 期刊論文 | Yang, Jingfa|Sun, Jianbo|Zhao, Jiang|Liang, Dehai| |
16 | Capsules with a hierarchical shell structure assembled by aminoglycosides and DNA via the kinetic path | 期刊論文 | Wang, Nan|Zhu, Lin|Qiu, Xiangyun|Liang, Dehai| |
17 | Effect of peptide charge distribution on the structure and kinetics of DNA complex | 期刊論文 | Deng, Yan|Qiu, Dong|Li, Baohui|Liang, Dehai| |
18 | Complexation between DNA and peptides with precisely controlled charge density and distribution | 期刊論文 | Wen, Hao|Su, Cui-cui|Niu, Lin|Liang, De-hai| |
19 | Dynamic assembly of DNA and polylysine mediated by electric energy | 期刊論文 | Qu, Wei|Zhou, Jihan|Zhao, Meiping|Liang, Dehai| |
20 | Local de-condensation of double-stranded DNA in oppositely charged polyelectrolyte as induced by spermidine | 期刊論文 | Zhou, Jihan|Yin, Yudan|Wen, Hao|Liang, Dehai| |
項目摘要
結構可控的DNA/RNA 複合物是開發安全、高效的非病毒類基因傳遞載體的首要條件。DNA/RNA在生理條件下攜帶有大量負電荷，可歸屬為聚電解質。計算機模擬結果表明，在電場存在下，聚電解質的構象會發生由線團到棒狀的轉變，而且抗衡離子的分布也會發生變化。這都會影響聚電解質的複合能力及所形成的複合物結構。本項目基於這一原理，提出利用毛細管電泳、微流體晶片電泳和板電泳來製備受電場調製的DNA/RNA 複合物；利用散射、TEM等物理手段來確定複合物結構和形態；並進一步建立複合物結構與傳遞效率之間的關係。本項目從聚電解質的基礎理論出發，引入電場這一能量因素，研究以DNA/RNA為代表的生物分子在非平衡態的複合規律，對製備高效傳遞載體有重要意義，對於透徹理解生命過程也有重要價值。
