中國科學院大學生命科學學院教授，博士生導師。主要從事生物醫學領域研究。
基本介紹
- 中文名：鐘良瑋
- 國籍：中國
- 民族：漢
- 職業：教授
- 畢業院校：瑞典卡羅林斯卡研究院
- 研究領域：生物醫學
個人簡介,學習經歷,工作經歷,學術成果,
個人簡介
中國科學院大學生命科學學院老師，教受生物化學、生物大分子、生物大分子討論課等課程。主要從事生物醫學領域研究。當前主要培養生物化學與分子生物學領域的博士研究生。
學習經歷
1975-09--1978-07 山西醫科大學 本科
1983-09--1984-07 北京大學 生物化學系 進修教師
1986-09--1989-07 山西醫科大學 碩士研究生
1996-06--2000-06 瑞典卡羅林斯卡研究院 博士研究生
工作經歷
1979-09—1994-01，山西醫科大學，助教、講師
1994-01—1996-05，瑞典卡羅林斯卡研究院，訪問學者
2000-06—2003-12，瑞典卡羅林斯卡研究院，博士後、Researcher
2003-12—， 中科院研究生院，副教授、教授
學術成果
Zhong, L., Arnér E. S. J., Ljung, J., Åslund, F. and Holmgren, A., Rat and calf thioredoxin reductase are homologous to glutathione reductase with a carboxyl-terminal elongation containing a conserved catalytically active penultimate selenocysteine residue, J. Biol. Chem. 273, 8571-8591 (1998)
Nordberg, J., Zhong, L., Holmgren, A. and Arnér, E. S. J., Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue, J. Biol. Chem. 273, 10835-10842 (1998)
Arnér, E. S. J., Zhong, L. and Holmgren, A., Preparation and assay of mammalian thioredoxin reductase, Methods Enzymol. 300, 226-239 (1999)
Zhong, L. and Holmgren, A., Essential role of selenium in catalytic activities of mammalian thioredoxin reductase revealed by characterization of recombinant enzymes with selenocysteine mutations. J. Biol. Chem. 275, 18121-18128 (2000)
Nordberg, J., Zhong, L., Holmgren, A. and Arnér, E. S. J., Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue, J. Biol. Chem. 273, 10835-10842 (1998)
Arnér, E. S. J., Zhong, L. and Holmgren, A., Preparation and assay of mammalian thioredoxin reductase, Methods Enzymol. 300, 226-239 (1999)
Zhong, L. and Holmgren, A., Essential role of selenium in catalytic activities of mammalian thioredoxin reductase revealed by characterization of recombinant enzymes with selenocysteine mutations. J. Biol. Chem. 275, 18121-18128 (2000)
Zhong, L., Arnér, E. S. J. and Holmgren, A., Structure and mechanism of mammalian thioredoxin reductase: the active site is a redox-active selenothiol/selenenylsulfide formed from the conserved cysteine-selenocysteine sequence. Proc. Natl. Acad. Sci. USA. 97, 5854-5859 (2000)
Zhong, L., Persson, K., Sandalova, T., Schneider, G. and Holmgren A., Purification, crystallization and preliminary crystallographic data for rat cytosolic selenocysteine-498 to cysteine mutant thioredoxin reductase. Acta Cryst. D56, 1191-1193 (2000)
Sandalova, T., Zhong, L., Lindqvist, Y., Holmgren, A. and Schneider, G. Three-dimensional structure of a mammalian thioredoxin reductase: Implications for mechanism and evolution of a selenocysteine-dependent enzyme. Proc. Natl. Acad. Sci. USA. 98, 9533-9538 (2001).
Zhong, L. and Holmgren, A. Mammalian thioredoxin reductases as hydroperoxide reductases. Methods Enzymol. 347. 236-243(2002)
Fang, J., Zhong, L., Zhao, R. and Holmgren, A., Ebselen: a thioredoxin reductase-dependent catalyst for α-tocopherol quinone reduction. Toxico. Appl. Pharm. 207, 103-9 (2005)
Zheng, Y., Zhong, L. * and Shen, X., Effect of selenium supplement on calcium signaling in cultured human umbilical vein endothelia cells. J. Cell Physiol. 1, 97-106(2005)
Wang, Y., Zhang, H., Holmgren, A., Tian W., and Zhong, L., Characterization of green tea polyphenols with mammalian thioredoxin reductase-inhibitory activity and growth-inhibitory activity toward cervical adenocarcinoma HeLa cells. Oncol Rep. 20(6):1479-87（2008）
Du, Y., Wu, Y., Cao X., Zhang H., Tian W., Holmgren, A. and Zhong L., Inhibition of Mammalian Thioredoxin Reductase by Black Tea and Its Constituents. Biochime. 91(3):434-44 (2009)
吳雲飛，鐘良瑋, 蛋白質同型半胱氨酸化：動脈粥樣硬化病理機制的研究進展。中國科學院研究生院學報，26(2):145-151（2009）
Cao, D., Zhang, Y., Zhang, H., Zhong, L. and Qian, X., Systematic Characterization of the Covalent Interactions between (-)-Epigallocatechin Gallate and Peptides at Physiological Conditions by Mass Spectrometry. Rapid Commun Mass Spectrum. 23(8):1147-57 (2009)
Lu, J., Zhong, L., Lonn, M., Burk, R., Hill, K. and Holmgren, A., Penultimete selenocysteine residue replaced by cysteine in thioredoxin reductase from selenium deficient rat liver. FASEB J. 23(8):2394-402 (2009)
Zhang, H. and Zhong L. Opposing regulation of histamine-induced calcium signaling by sodium selenite and ebselen via alterations of thiol redox status. Eur J Pharmacol. 626(2-3):276-82. (2010)
翟光磊，鐘良瑋，N-末端親和標籤可增進人硫氧還蛋白-1對氧化劑的敏感性.中國生物化學與分子生物學報.26(3):234-253 (2010)
Wu, Y., Yang, L. and Zhong, L., Decreased serum levels of thioredoxin in patients with coronary artery disease plus hyperhomocysteinemia is strongly associated with the disease severity. Atherosclerosis. 212(1):351-5 (2010)
Zhang H, Cao D, Cui W, Ji M, Qian X, Zhong L. Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallocatechin-3-gallate. Free Radic Biol Med. 49(12):2010-8 (2010)
Huang J. and Zhong L. Thioredoxin Reductase. In Selenoproteins and Mimics (Junqiu Liu, Guimin Luo, Eds) Zhejiang University Press, co-published with Springer . pp 41-64 (2011)
Wang P, Wu Y, Li X, Ma X, Zhong L. Thioredoxin and Thioredoxin Reductase Control Tissue Factor Activity by Thiol-Redox-Dependent Mechanism. J Biol Chem.288(5):3346-58 (2013)
Huang J, Xu J, Tian L, Zhong L. A thioredoxin reductase and/or thioredoxin system-based mechanism for antioxidant effects of ambroxol. Biochimie. 97: 92-103 (2014).
Liu H., Liu J., Huang J., Zhong L., Xu J. Unique association of adiponectin isoforms with serum cytokines and redox molecules in patients with chronic obstructive pulmonary disease. Chinese Medical Journal 126 (17): 3383-84 (2013)
Qina H., Zhang X., Ye F., Zhong L. High-fat diet-induced changes in liver thioredoxin and thioredoxin reductase as a novel feature of insulin resistance. FEBS Open Bio. 4: 928-936 (2014)
Zhong, L., Persson, K., Sandalova, T., Schneider, G. and Holmgren A., Purification, crystallization and preliminary crystallographic data for rat cytosolic selenocysteine-498 to cysteine mutant thioredoxin reductase. Acta Cryst. D56, 1191-1193 (2000)
Sandalova, T., Zhong, L., Lindqvist, Y., Holmgren, A. and Schneider, G. Three-dimensional structure of a mammalian thioredoxin reductase: Implications for mechanism and evolution of a selenocysteine-dependent enzyme. Proc. Natl. Acad. Sci. USA. 98, 9533-9538 (2001).
Zhong, L. and Holmgren, A. Mammalian thioredoxin reductases as hydroperoxide reductases. Methods Enzymol. 347. 236-243(2002)
Fang, J., Zhong, L., Zhao, R. and Holmgren, A., Ebselen: a thioredoxin reductase-dependent catalyst for α-tocopherol quinone reduction. Toxico. Appl. Pharm. 207, 103-9 (2005)
Zheng, Y., Zhong, L. * and Shen, X., Effect of selenium supplement on calcium signaling in cultured human umbilical vein endothelia cells. J. Cell Physiol. 1, 97-106(2005)
Wang, Y., Zhang, H., Holmgren, A., Tian W., and Zhong, L., Characterization of green tea polyphenols with mammalian thioredoxin reductase-inhibitory activity and growth-inhibitory activity toward cervical adenocarcinoma HeLa cells. Oncol Rep. 20(6):1479-87（2008）
Du, Y., Wu, Y., Cao X., Zhang H., Tian W., Holmgren, A. and Zhong L., Inhibition of Mammalian Thioredoxin Reductase by Black Tea and Its Constituents. Biochime. 91(3):434-44 (2009)
吳雲飛，鐘良瑋, 蛋白質同型半胱氨酸化：動脈粥樣硬化病理機制的研究進展。中國科學院研究生院學報，26(2):145-151（2009）
Cao, D., Zhang, Y., Zhang, H., Zhong, L. and Qian, X., Systematic Characterization of the Covalent Interactions between (-)-Epigallocatechin Gallate and Peptides at Physiological Conditions by Mass Spectrometry. Rapid Commun Mass Spectrum. 23(8):1147-57 (2009)
Lu, J., Zhong, L., Lonn, M., Burk, R., Hill, K. and Holmgren, A., Penultimete selenocysteine residue replaced by cysteine in thioredoxin reductase from selenium deficient rat liver. FASEB J. 23(8):2394-402 (2009)
Zhang, H. and Zhong L. Opposing regulation of histamine-induced calcium signaling by sodium selenite and ebselen via alterations of thiol redox status. Eur J Pharmacol. 626(2-3):276-82. (2010)
翟光磊，鐘良瑋，N-末端親和標籤可增進人硫氧還蛋白-1對氧化劑的敏感性.中國生物化學與分子生物學報.26(3):234-253 (2010)
Wu, Y., Yang, L. and Zhong, L., Decreased serum levels of thioredoxin in patients with coronary artery disease plus hyperhomocysteinemia is strongly associated with the disease severity. Atherosclerosis. 212(1):351-5 (2010)
Zhang H, Cao D, Cui W, Ji M, Qian X, Zhong L. Molecular bases of thioredoxin and thioredoxin reductase-mediated prooxidant actions of (-)-epigallocatechin-3-gallate. Free Radic Biol Med. 49(12):2010-8 (2010)
Huang J. and Zhong L. Thioredoxin Reductase. In Selenoproteins and Mimics (Junqiu Liu, Guimin Luo, Eds) Zhejiang University Press, co-published with Springer . pp 41-64 (2011)
Wang P, Wu Y, Li X, Ma X, Zhong L. Thioredoxin and Thioredoxin Reductase Control Tissue Factor Activity by Thiol-Redox-Dependent Mechanism. J Biol Chem.288(5):3346-58 (2013)
Huang J, Xu J, Tian L, Zhong L. A thioredoxin reductase and/or thioredoxin system-based mechanism for antioxidant effects of ambroxol. Biochimie. 97: 92-103 (2014).
Liu H., Liu J., Huang J., Zhong L., Xu J. Unique association of adiponectin isoforms with serum cytokines and redox molecules in patients with chronic obstructive pulmonary disease. Chinese Medical Journal 126 (17): 3383-84 (2013)
Qina H., Zhang X., Ye F., Zhong L. High-fat diet-induced changes in liver thioredoxin and thioredoxin reductase as a novel feature of insulin resistance. FEBS Open Bio. 4: 928-936 (2014)
