《酶對核酸進行化學修飾的動力學機理》是趙新生為項目負責人，北京大學為依託單位的重點項目。
基本介紹
- 中文名：酶對核酸進行化學修飾的動力學機理
- 項目類別 ：重點項目
- 項目負責人：趙新生
- 依託單位 ：北京大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Single-Molecule Detection Reveals Knot Sliding in TrmD Denaturation | 期刊論文 | Wang, Peng，Yang, Lijiang，Liu, Pengcheng，Gao, Yi Qin(*)，Zhao, Xin Sheng(*) |
2 | Dual reorientation relaxation routes of water molecules in oxyanion's hydration shell: A molecular geometry perspective | 期刊論文 | Xie, Wen Jun(#)，Yang, Yi Isaac，Gao, Yi Qin(*) |
3 | 基於鹵鍵的謝爾賓斯基三角分形自組裝的模擬研究(英文) | 期刊論文 | 張珍，謝文俊，楊奕，孫耿，高毅勤(*) |
4 | Ion Pairing in Alkali Nitrate Electrolyte Solutions | 期刊論文 | Xie, Wen Jun(#)，Zhang, Zhen，Gao, Yi Qin(*) |
5 | Determination of equilibrium constant and relative brightness in fluorescence correlation spectroscopy by considering third-order correlations | 期刊論文 | Zhenqin Wu(#)，Huimin Bi，Sichen Pan，Lingyi Meng，Xin Sheng Zhao(*) |
6 | Scanning Single-Molecule Fluorescence Correlation Spectroscopy Enables Kinetics Study of DNA Hairpin Folding with a Time Window from Microseconds to Seconds | 期刊論文 | Bi, Huimin(#)，Yin, Yandong，Pan, Bailong，Li, Geng，Zhao, Xin Sheng(*) |
7 | Emergence of Solvent-Separated Na+-Cl- Ion Pair in Salt Water: Photoelectron Spectroscopy and Theoretical Calculations | 期刊論文 | Hou, Gao-Lei(#)，Liu, Cheng-Wen，Li, Ren-Zhong，Xu, Hong-Guang，Gao, Yi Qin(*)，Zheng, Wei-Jun(*) |
8 | Accurate placement of substrate RNA by Gar1 in H/ACA RNA-guided pseudouridylation | 期刊論文 | Wang, Peng(#)，Yang, Lijiang，Gao, Yi Qin(*)，Zhao, Xin Sheng(*) |
9 | DNA Methylation Landscape Reflects the Spatial Organization of Chromatin in Different Cells | 期刊論文 | Ling Zhang(#)，Wen Jun Xie，Sirui Liu，Luming Meng，Chan Gu，Yi Qin Gao(*) |
10 | Direct Observation of the Uptake of Outer Membrane Proteins by the Periplasmic Chaperone Skp | 期刊論文 | Lyu, Zhi-Xin(#)，Shao, Qiang，Gao, Yi Qin(*)，Zhao, Xin Sheng(*) |
11 | Structural Modeling of Chromatin Integrates Genome Features and Reveals Chromosome Folding Principle | 期刊論文 | Wen Jun Xie(#)，Luming Meng，Sirui Liu，Ling Zhang，Xiaoxia Cai，Yi Qin Gao(*) |
12 | Determination of Equilibrium Constant and Relative Brightness in FRET-FCS by Including the Third-Order Correlations. | 期刊論文 | Lingyi Meng(#)，Shanshan He，Xin Sheng Zhao(*) |
13 | Structural Flexibility and Conformation Features of Cyclic Dinucleotides in Aqueous Solutions | 期刊論文 | Che, Xing(#)，Zhang, Jun，Zhu, Yanyu，Yang, Lijiang，Quan, Hui，Gao, Yi Qin(*) |
14 | Adsorption of water molecules on sodium chloride trimer | 期刊論文 | Liu, Cheng-Wen，Hou, Gao-Lei，Zheng, Wei-Jun，Gao, Yi Qin(*) |
15 | Understanding the Microsolvation of Salts in Molecular Clusters | 期刊論文 | Liu, Cheng-Wen(#)，Gao, Yi Qin(*) |
16 | Kinetic insights into the temperature dependence of DNA strand cleavage and religation by topoisomerase III from the hyperthermophile Sulfolobus solfataricus | 期刊論文 | Zhang, Junhua(#)，Pan, Bailong，Li, Zhimeng，Zhao, Xin Sheng(*)，Huang, Li(*) |
17 | Simulation Studies of Protein and Small Molecule Interactions and Reaction | 期刊論文 | Lijiang Yang(#)，Jun Zhang，Xing Che，Yi Qin Gao(*) |
18 | From Thermodynamics to Kinetics: Enhanced Sampling of Rare Events | 期刊論文 | Yang, Lijiang(#)，Liu, Cheng-Wen，Shao, Qiang，Zhang, Jun，Gao, Yi Qin(*) |
19 | How Quickly Can a beta-Hairpin Fold from Its Transition State? | 期刊論文 | Markiewicz, Beatrice N.，Yang, Lijiang，Culik, Robert M.，Gao, Yi Qin(*)，Gai, Feng(*) |
20 | Molecular Dynamic Simulations of the Effects of Trimethylamine-N-oxide/Urea Mixture on the Hydration of Single-Walled Carbon Nanotube Interiors | 期刊論文 | Yang Li-Jiang(#)，Gao Yi-Qin(*) |
21 | Kinetic and thermodynamic characterization of the reaction pathway of box H/ACA RNA-guided pseudouridine formation | 期刊論文 | Yang, Xinxing，Duan, Jingqi，Li, Shuang，Wang, Peng，Ma, Shoucai，Ye, Keqiong(*)，Zhao, Xin Sheng(*) |
22 | DNA Structural Correlation in Short and Long Ranges | 期刊論文 | Gu, Chan(#)，Zhang, Jun，Yang, Y. Isaac，Chen, Xi，Ge, Hao，Sun, Yujie，Su, Xiaodong，Yang, Lijiang，Xie, Sunney，Gao, Yi Qin(*) |
23 | Stable Salt-Water Cluster Structures Reflect the Delicate Competition between Ion-Water and Water-Water Interactions | 期刊論文 | Liu, Cheng-Wen(#)，Wang, Feng，Yang, Lijiang，Li, Xin-Zheng，Zheng, Wei-Jun(*)，Gao, Yi Qin(*) |
24 | Amplitude of Relaxations in Fluorescence Correlation Spectroscopy for Fluorophores That Diffuse Together | 期刊論文 | Yin, Yandong(#)，Yuan, Rongfeng，Zhao, Xin Sheng(*) |
25 | Dynamics of spontaneous flipping of a mismatched base in DNA duplex | 期刊論文 | Yin, Yandong，Yang, Lijiang，Zheng, Guanqun，Gu, Chan，Yi, Chengqi，He, Chuan，Gao, Yi Qin(*)，Zhao, Xin Sheng(*) |
26 | Periplasmic quality control in biogenesis of outer membrane proteins | 期刊論文 | Lyu, Zhi Xin(#)，Zhao, Xin Sheng(*) |
項目摘要
生命個體的發育主要取決於遺傳基因DNA序列，但其複雜性和多樣性不能僅由生物學中心法則解釋。在生命個體的發育過程中生物大分子的化學修飾對決定細胞命運起著關鍵的調控作用。具有遺傳性的生物大分子化學修飾的研究是當前生命科學研究的一個前沿課題。本申請的研究內容是酶對核酸（DNA和RNA）進行化學修飾和去修飾的動力學機理。由於以前研究手段的制約，關於核酸化學修飾動力學機理的研究尚處在初期。我們擬將實驗（單分子探測）和理論（分子動力學模擬）結合在一起，採用先進的研究手段深入探究酶對核酸進行化學修飾的動力學過程，以便清楚地了解酶如何和以多快的速率發現被修飾和去修飾的位點，以及酶在發現位點後對其進行化學修飾的分子機制和反應動力學。這些研究將揭示相關生物分子反應的化學本質，有助於更深刻地認識相應的生物過程。
