郭占雲

郭占雲，1972年生，河北灤南人，中國科學院上海生物化學研究所博士，現任同濟大學生命科學與技術學院教授，博士生導師。

基本介紹

• 中文名：郭占雲
• 畢業院校：中國科學院上海生物化學研究所
• 學位/學歷：博士
• 專業方向：蛋白質與多肽
• 職務：同濟大學博士生導師

研究領域

生物化學

蛋白質與多肽

人物經歷

1996年畢業於吉林大學分子生物學系，獲學士學位。因成績優異，同年免試進入中國科學院上海生物化學研究所攻讀博士學 位，在馮佑民研究員實驗室從事胰島素超家族多肽研究。2001年8月獲生物化學與分子生物學博士學位。2001-2003年在生物化學與細胞生物學研究所 工作，歷任助理研究員和副研究員。2003年9月到美國Dartmouth College醫學院生物化學系從事博士後研究，主要研究膽固醇酯合酶1（ACAT1）結構與功能。2006年底回國到同濟大學蛋白質研究所工作。已在J Biol Chem, Biochemistry, Protein Science等國內外知名科技期刊上以第一作者（含共同第一作者）或通訊作者（含共同通訊作者）發表SCI論文近30篇。目前作為項目（課題）負責人正 主持國家自然科學基金面上項目2項；國家“重大新藥創製”科技重大專項（候選藥物）1項；國家重點基礎研究發展計畫（973）課題一項。

發表論文

1. Luo X, Bathgate RA, Zhang WJ, Liu YL, Shao XX, Wade JD*, Guo ZY*. (2010) Design and recombinant expression of insulin-like peptide 5 (INSL5) precursors and the preparation of mature human INSL5. Amino Acids, in minor revision. (* corresponding authors)

2. Wu XC, Zhou M, Peng C, Shao XX, Guo ZY*, Chi CW* (2010) Novel conopeptides in a form of disulfide-crosslinked dimer. Peptides, accepted. (* corresponding authors)

3. Wang ZQ, Zhou ZM, Guo ZY*, Chi CW* (2010) Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase. Acta Biochim Biophys Sin,accepted (* corresponding authors)

4. Zhang WJ, Luo X, Guo ZY* (2010) In Vitro Degradation of Insulin-like Peptide 3 by Insulin-Degrading Enzyme. Protein J, published online (* corresponding author)

5. Luo X, Bathgate RA, Liu YL, Shao XX, Wade JD*, Guo ZY*. (2009) Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion tomature human INSL3. FEBS J,276: 5203-5211. (*corresponding authors)

6. Yuan DD, Liu L, Shao XX, Peng C, Chi CW*, Guo ZY*(2009) New conotoxins define the novel I3-superfamily. Peptides,30: 861-865. (*corresponding authors)

7. Liu YL, Jiang S, Ke ZM, Wu HS, Chi CW, Guo ZY*(2009) Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production. Carbohyd Res,344: 815-819. (*corresponding author)

8. Yuan DD, Liu L, Shao XX, Peng C, Chi CW*, Guo ZY*(2008) Isolation and cloning of a conotoxin with a novel cysteine pattern from Conus caracteristicus. Peptides,29: 1521-1525. (*corresponding authors)

9. Qian J,Guo ZY*, Chi CW*. (2008) Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity. Acta Biochim Biophys Sin,40: 174-181. (*corresponding authors)

10. Zhang ZJ, Wu L, Qiao ZS, Qiao MQ, Feng YM*, Guo ZY*(2008) Contribution of the conservedA16Leu to insulin foldability. Protein J,27: 192-196. (*corresponding authors)

11. Guo ZY*, Qiao ZS*, Feng YM (2008) The in vitro oxidative folding of the insulin superfamily.AntioxidRedox Signal,10: 127-139. (*co-first authors)

12. Guo ZY, Chang CCY, Chang TY (2007) Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1.Biochemistry,46: 10063-10071.

13. Wang ZQ, Han YH, Shao XX, Chi CW*, Guo ZY* (2007) Molecular cloning, expression, andcharacterization of protein disulfide isomerase from Conus marmoreus. FEBS J,274: 4778-4787.(*corresponding authors)

14. Guo ZY, Lin S, Henien JA, Chang CY, Chang TY (2005) The active site His-460 of human acyl-coenzyme A: cholesterol acyltransferase 1 resides in a hitherto undisclosed transmembrane domain.J Biol Chem, 280: 37814-37826.

15. Guo ZY,Chang CY, Lu X, Chen J, Li BL, Chang TY (2005) The disulfide linkage and the free sulfhydryl accessibility of acyl-coenzyme A:cholesterol acyltransferase 1 as studied by using mPEG5000-maleimide. Biochemistry,44: 6537-6546.

16. Guo ZY,Zhang Z, Jia XY, Tang YH, Feng YM (2005) Mutational analysis of the absolutely conserved B8Gly: consequence on foldability and activity of insulin. Acta Biochim Biophys Sin,37: 673-679.

17. Guo ZY,Jia XY, Feng YM (2004) Replacement of the interchain disulfide-forming amino acidsA7and B7 by glutamate impairs the structure and activity of insulin. Biol Chem,385:1171-1175.

18. Guo ZY,Wang S, Tang YH, Feng YM (2004) Mutagenesis of the three conserved valine residues:consequence on the foldability of insulin. Biochim Biophys Acta,1699: 103-109.

19. Jia XY*, Guo ZY*, Wang Y, Xu Y, Duan SS, Feng YM (2003) Peptide models of four possibleinsulin folding intermediates with two disulfides. Protein Sci,12: 2412-2419. (*co- first authors)

20. Wang S*, Guo ZY*, Shen L, Feng YM (2003) Refolding of the amphioxus insulin-like peptide:implications of a bifurcating evolution of the different folding behaviors of insulin and insulin-like

growth factor 1. Biochemistry,42: 9687-9693. (*co-first authors)

21. Guo ZY, Tang YH, Wang S, Feng YM (2003) Contribution of the absolutely conserved B8Glytothe foldability of insulin. Biol Chem, 384: 805-809.

22. Yan H*, Guo ZY*, Gong YW, Xi D, Feng YM (2003) A peptide model of insulin foldingintermediate with one disulfide. Protein Sci,12: 768-775. (*co-first authors)

23. Guo ZY, Shen L, Gu W, Wu AZ, Ma JG., Feng YM (2002) In vitro evolution of amphioxus insulin-like peptide to mammalian insulin. Biochemistry,41: 10603-10607.

24. Guo ZY, Shen L, Feng YM (2002) The different energetic state of the intra A-chain/ domaindisulfide of insulin and insulin-like growth factor 1 is mainly controlled by their B-chain/domain.Biochemistry,41: 10585-10592.

25. Guo ZY, Shen L, Feng YM (2002) The different folding behavior of insulin and insulin-like growthfactor 1 is mainly controlled by their B-chain/domain. Biochemistry,41: 1556-1567.

26. Guo ZY, Feng YM (2002) The thermodynamic stability of insulin disulfides is not affected by theC-domain of insulin-like growth factor 1. Sci China (Series C),45: 245-250.

27. Guo ZY, Feng YM (2001) Effects of cysteine to serine substitutions in the two inter-chain disulfidebonds of insulin. Biol Chem,382: 443-448

28. Guo ZY, Tang YH, Zhang Z, Feng YM (2001) Mutational analysis of the three conserved valineresidues of insulin and a proposal of “isosteric residue”. IUBMB Life,52: 309-314.

29. Guo ZY, Qiao ZS, Feng YM (2001) Unfolding of recombinant single-chain insulin in denaturantscontaining thiol reagents. Acta Biochim Biophys Sin,33: 65-70.

30. Shen L*, Guo ZY*, Chen Y, Liu LY, Feng YM (2001) Expression, purification, characterizationofampioxus insulin-like peptide and preparation of polyclonal antibody to it.

ActaBiochimBiophysSin,33: 629-633. (*co-first authors)